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Protein

Calnexin homolog

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Calcium; via carbonyl oxygenBy similarity
Metal bindingi75 – 751Calcium; via carbonyl oxygenBy similarity
Binding sitei122 – 1221CarbohydrateBy similarity
Binding sitei124 – 1241CarbohydrateBy similarity
Binding sitei144 – 1441CarbohydrateBy similarity
Binding sitei175 – 1751CarbohydrateBy similarity
Metal bindingi390 – 3901CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calnexin homolog
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 480454LumenalSequence analysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence analysisAdd
BLAST
Topological domaini502 – 55150CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 551525Calnexin homologPRO_0000004205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi118 ↔ 153By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence analysis
Disulfide bondi315 ↔ 321By similarity
Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO82709.

Expressioni

Tissue specificityi

In vegetative and flowering tissues.

Structurei

3D structure databases

ProteinModelPortaliO82709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati235 – 246121-1Add
BLAST
Repeati252 – 263121-2Add
BLAST
Repeati271 – 282121-3Add
BLAST
Repeati289 – 299111-4Add
BLAST
Repeati303 – 313112-1Add
BLAST
Repeati322 – 332112-2Add
BLAST
Repeati336 – 346112-3Add
BLAST
Repeati350 – 360112-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni233 – 364132P domain (Extended arm)By similarityAdd
BLAST
Regioni235 – 299654 X approximate repeatsAdd
BLAST
Regioni303 – 360584 X approximate repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDRKEIPLA MGLLAVLLFF VASSSSFHLV RASDEVDDAI FYESFDEDFD
60 70 80 90 100
NRWIVSGKEE YNGVWKHSKS EGHDDFGLLV SEPARKYAIV KELDAPVSLK
110 120 130 140 150
DGTVVLQFET RLQNGLECGG AYIKYLQTQE SGWKPKGFDN ESGYSIMFGP
160 170 180 190 200
DRCGATNKVH FIFRHKNPKT GKHVEHHLKF PPSVPSDKLS HVYTAVLKDD
210 220 230 240 250
NEVSILIDGE EKKKANFLSS EDFEPALIPS KTIPDPDDKK PEDWDERAKI
260 270 280 290 300
PDPEAVKPED WDEDAPREII DEEAEKPEPW LDHEPEVDDP EAKPEDWDDE
310 320 330 340 350
EDGEWEAPKI ENPKCEAAPG CGEWKRPTKS NPAYKGKWSA PYIDNPNYKG
360 370 380 390 400
IWKPQEIPNP EYFELEKPDF EPIAAIGIEI WTMQDGILFD NVLIAKDDKI
410 420 430 440 450
AESYRETTWK PKFNIEKEKQ KHEEEAAAAA AARSESEGIA GIQKKAFDLL
460 470 480 490 500
YKIADIAFLS GQKEKIIEII EKGEKQPNLT IGIIVSVVIV FVSIFFRLIF
510 520 530 540 550
GGKKPANVEA NVEKKKTNTE TTSKQDGGEK EDNKEKEETA NPPRRRPKRD

N
Length:551
Mass (Da):62,532
Last modified:November 1, 1998 - v1
Checksum:i25A5F5F13B989365
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17329 mRNA. Translation: CAA76741.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17329 mRNA. Translation: CAA76741.1.

3D structure databases

ProteinModelPortaliO82709.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO82709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Calnexin from Pisum sativum: cloning of the cDNA and characterization of the encoded protein."
    Ehtesham N.Z., Phan T.N., Gaikwad A., Sopory S.K., Tuteja N.
    DNA Cell Biol. 18:853-862(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.

Entry informationi

Entry nameiCALX_PEA
AccessioniPrimary (citable) accession number: O82709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.