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O82663

- SDHA1_ARATH

UniProt

O82663 - SDHA1_ARATH

Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial

Gene

SDH1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei264 – 2641FADBy similarity
    Binding sitei285 – 2851SubstrateBy similarity
    Binding sitei297 – 2971SubstrateBy similarity
    Active sitei329 – 3291Proton acceptorBy similarity
    Binding sitei396 – 3961SubstrateBy similarity
    Binding sitei430 – 4301FADBy similarity
    Binding sitei441 – 4411SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi56 – 616FADBy similarity
    Nucleotide bindingi79 – 9416FADBy similarityAdd
    BLAST
    Nucleotide bindingi446 – 4472FADBy similarity

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. cobalt ion binding Source: TAIR
    3. flavin adenine dinucleotide binding Source: InterPro
    4. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    5. succinate dehydrogenase activity Source: TAIR

    GO - Biological processi

    1. mitochondrial electron transport, succinate to ubiquinone Source: TAIR
    2. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT5G66760-MONOMER.
    MetaCyc:AT5G66760-MONOMER.
    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Flavoprotein subunit 1 of complex II
    Short name:
    FP
    Gene namesi
    Name:SDH1-1
    Ordered Locus Names:At5g66760
    ORF Names:MSN2.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G66760.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. mitochondrial respiratory chain complex II Source: TAIR
    3. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
    BLAST
    Chaini33 – 634602Succinate dehydrogenase [ubiquinone] flavoprotein subunit 1, mitochondrialPRO_0000158659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei87 – 871Tele-8alpha-FAD histidineBy similarity

    Proteomic databases

    PaxDbiO82663.
    PRIDEiO82663.

    Expressioni

    Tissue specificityi

    Ubiquitous. Preferentially expressed in flowers and inflorescences.1 Publication

    Gene expression databases

    GenevestigatoriO82663.

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

    Protein-protein interaction databases

    IntActiO82663. 2 interactions.
    MINTiMINT-8063231.
    STRINGi3702.AT5G66760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82663.
    SMRiO82663. Positions 41-634.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1053.
    HOGENOMiHOG000160475.
    InParanoidiO82663.
    KOiK00234.
    OMAiRDGPILQ.
    PhylomeDBiO82663.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O82663-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWRCVSRGFR APASKTSSLF DGVSGSRFSR FFSTGSTDTR SSYTIVDHTY    50
    DAVVVGAGGA GLRAAIGLSE HGFNTACITK LFPTRSHTVA AQGGINAALG 100
    NMSEDDWRWH MYDTVKGSDW LGDQDAIQYM CREAPKAVIE LENYGLPFSR 150
    TEEGKIYQRA FGGQSLDFGK GGQAYRCACA ADRTGHALLH TLYGQAMKHN 200
    TQFFVEYFAL DLLMASDGSC QGVIALNMED GTLHRFRSSQ TILATGGYGR 250
    AYFSATSAHT CTGDGNAMVA RAGLPLQDLE FVQFHPTGIY GAGCLITEGS 300
    RGEGGILRNS EGERFMERYA PTAKDLASRD VVSRSMTMEI REGRGVGPHK 350
    DHIYLHLNHL PPEVLKERLP GISETAAIFA GVDVTKEPIP VLPTVHYNMG 400
    GIPTNYHGEV VTIKGDDPDA VIPGLMAAGE AACASVHGAN RLGANSLLDI 450
    VVFGRACANR VAEISKPGEK QKPLEKDAGE KTIAWLDRLR NSNGSLPTST 500
    IRLNMQRIMQ NNAAVFRTQE TLEEGCQLID KAWESFGDVQ VKDRSMIWNS 550
    DLIETLELEN LLINASITMH SAEARKESRG AHAREDFTKR EDGEWMKHTL 600
    GYWEDEKVRL DYRPVHMDTL DDEIDTFPPK ARVY 634
    Length:634
    Mass (Da):69,656
    Last modified:November 1, 1998 - v1
    Checksum:iAECE471C7AD43B84
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001809 mRNA. Translation: CAA05025.1.
    AB018119 Genomic DNA. Translation: BAA97282.1.
    CP002688 Genomic DNA. Translation: AED98260.1.
    AF367341 mRNA. Translation: AAK32928.1.
    AY045674 mRNA. Translation: AAK74032.1.
    AF436833 mRNA. Translation: AAL32015.1.
    AY124812 mRNA. Translation: AAM70521.1.
    PIRiT51815.
    RefSeqiNP_201477.1. NM_126074.2.
    UniGeneiAt.22655.
    At.67108.

    Genome annotation databases

    EnsemblPlantsiAT5G66760.1; AT5G66760.1; AT5G66760.
    GeneIDi836809.
    KEGGiath:AT5G66760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001809 mRNA. Translation: CAA05025.1 .
    AB018119 Genomic DNA. Translation: BAA97282.1 .
    CP002688 Genomic DNA. Translation: AED98260.1 .
    AF367341 mRNA. Translation: AAK32928.1 .
    AY045674 mRNA. Translation: AAK74032.1 .
    AF436833 mRNA. Translation: AAL32015.1 .
    AY124812 mRNA. Translation: AAM70521.1 .
    PIRi T51815.
    RefSeqi NP_201477.1. NM_126074.2.
    UniGenei At.22655.
    At.67108.

    3D structure databases

    ProteinModelPortali O82663.
    SMRi O82663. Positions 41-634.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O82663. 2 interactions.
    MINTi MINT-8063231.
    STRINGi 3702.AT5G66760.1-P.

    Proteomic databases

    PaxDbi O82663.
    PRIDEi O82663.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G66760.1 ; AT5G66760.1 ; AT5G66760 .
    GeneIDi 836809.
    KEGGi ath:AT5G66760.

    Organism-specific databases

    GeneFarmi 2175. 174.
    TAIRi AT5G66760.

    Phylogenomic databases

    eggNOGi COG1053.
    HOGENOMi HOG000160475.
    InParanoidi O82663.
    KOi K00234.
    OMAi RDGPILQ.
    PhylomeDBi O82663.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .
    BioCyci ARA:AT5G66760-MONOMER.
    MetaCyc:AT5G66760-MONOMER.

    Miscellaneous databases

    PROi O82663.

    Gene expression databases

    Genevestigatori O82663.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of succinyl-CoA-ligase from Arabidopsis thaliana."
      Machuy N., Klein M., Mueller-Roeber B.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Proteomic approach to identify novel mitochondrial proteins in Arabidopsis."
      Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.
      Plant Physiol. 127:1694-1710(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 160-170.
      Tissue: Leaf and Stem.
    6. "The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana."
      Figueroa P., Leon G., Elorza A., Holuigue L., Araya A., Jordana X.
      Plant Mol. Biol. 50:725-734(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, TISSUE SPECIFICITY.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiSDHA1_ARATH
    AccessioniPrimary (citable) accession number: O82663
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3