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Protein

Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial

Gene

At2g20420

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g22800), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (AXX17_At2g15740), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (At2g20420), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, Succinate--CoA ligase [ADP-forming] subunit alpha-2, mitochondrial (At5g23250), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g08090), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (MKD15.11), Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrial (At5g08300)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74ATPUniRule annotation1
Binding sitei141ATPUniRule annotation1
Metal bindingi233MagnesiumUniRule annotation1
Metal bindingi247MagnesiumUniRule annotation1
Binding sitei298Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 83ATPUniRule annotation3

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • succinate-CoA ligase (ADP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G20420-MONOMER
ReactomeiR-ATH-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase beta chainUniRule annotation
Short name:
SCS-betaUniRule annotation
Gene namesi
Ordered Locus Names:At2g20420
ORF Names:F11A3.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G20420
TAIRilocus:2039037 AT2G20420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26Mitochondrion1 PublicationAdd BLAST26
ChainiPRO_000003336227 – 421Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialAdd BLAST395

Proteomic databases

PaxDbiO82662
PRIDEiO82662

Expressioni

Gene expression databases

ExpressionAtlasiO82662 baseline and differential
GenevisibleiO82662 AT

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT2G20420.1

Structurei

3D structure databases

ProteinModelPortaliO82662
SMRiO82662
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 278ATP-graspUniRule annotationAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 357Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799 Eukaryota
COG0045 LUCA
HOGENOMiHOG000007059
InParanoidiO82662
KOiK01900
OMAiLCMDAKF
OrthoDBiEOG09360AMS
PhylomeDBiO82662

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.40.50.261, 1 hit
HAMAPiMF_00558 Succ_CoA_beta, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013650 ATP-grasp_succ-CoA_synth-type
IPR013815 ATP_grasp_subdomain_1
IPR005811 CoA_ligase
IPR017866 Succ-CoA_synthase_bsu_CS
IPR005809 Succ_CoA_synthase_bsu
IPR016102 Succinyl-CoA_synth-like
PANTHERiPTHR11815 PTHR11815, 1 hit
PfamiView protein in Pfam
PF08442 ATP-grasp_2, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001554 SucCS_beta, 1 hit
SUPFAMiSSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01016 sucCoAbeta, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS01217 SUCCINYL_COA_LIG_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLVNKLVS RSLSISGKWQ NQQLRRLNIH EYQGAELMGK YGVNVPKGVA
60 70 80 90 100
ASSLEEVKKA IQDVFPNESE LVVKSQILAG GRGLGTFKSG LKGGVHIVKR
110 120 130 140 150
DEAEEIAGKM LGQVLVTKQT GPQGKVVSKV YLCEKLSLVN EMYFSIILDR
160 170 180 190 200
KSAGPLIIAC KKGGTSIEDL AEKFPDMIIK VPIDVFAGIT DEDAAKVVDG
210 220 230 240 250
LAPKAADRKD SIEQVKKLYE LFRKTDCTML EINPLAETST NQLVAADAKL
260 270 280 290 300
NFDDNAAFRQ KEVFAMRDPT QEDPREVAAA KVDLNYIGLD GEIGCMVNGA
310 320 330 340 350
GLAMATMDII KLHGGTPANF LDVGGNASEH QVVEAFKILT SDDKVKAILV
360 370 380 390 400
NIFGGIMKCD VIASGIVNAA KEVALKVPVV VRLEGTNVEQ GKRILKESGM
410 420
KLITADDLDD AAEKAVKALA H
Length:421
Mass (Da):45,346
Last modified:November 1, 1998 - v1
Checksum:i44F7491FA9029EC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001808 mRNA Translation: CAA05024.1
AC006569 Genomic DNA Translation: AAM15283.1
CP002685 Genomic DNA Translation: AEC07005.1
AY099707 mRNA Translation: AAM20558.1
AY128889 mRNA Translation: AAM91289.1
PIRiT51809
RefSeqiNP_179632.1, NM_127601.4
UniGeneiAt.21151

Genome annotation databases

EnsemblPlantsiAT2G20420.1; AT2G20420.1; AT2G20420
GeneIDi816561
GrameneiAT2G20420.1; AT2G20420.1; AT2G20420
KEGGiath:AT2G20420

Similar proteinsi

Entry informationi

Entry nameiSUCB_ARATH
AccessioniPrimary (citable) accession number: O82662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health