ID   P2SAF_ARATH             Reviewed;         403 AA.
AC   O82660; Q56Z72;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=Photosystem II stability/assembly factor HCF136, chloroplastic;
DE   Flags: Precursor;
GN   Name=HCF136; OrderedLocusNames=At5g23120; ORFNames=MYJ24.11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=cv. Wassilewskija; TISSUE=Leaf;
RX   PubMed=9736608; DOI=10.1093/emboj/17.18.5286;
RA   Meurer J., Pluecken H., Kowallik K.V., Westhoff P.;
RT   "A nuclear-encoded protein of prokaryotic origin is essential for the
RT   stability of photosystem II in Arabidopsis thaliana.";
RL   EMBO J. 17:5286-5297(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA   Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT   features of the regions of 1,044,062 bp covered by thirteen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:291-300(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-403.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   PROTEIN SEQUENCE OF 79-93, AND SUBCELLULAR LOCATION.
RX   PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA   Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA   Kieselbach T.;
RT   "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL   J. Biol. Chem. 277:8354-8365(2002).
RN   [7]
RP   PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=11826309; DOI=10.1105/tpc.010304;
RA   Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA   Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA   van Wijk K.J.;
RT   "Central functions of the lumenal and peripheral thylakoid proteome of
RT   Arabidopsis determined by experimentation and genome-wide prediction.";
RL   Plant Cell 14:211-236(2002).
RN   [8]
RP   IN VITRO IMPORT INTO PEA CHLOROPLASTS, AND MUTAGENESIS OF 55-ARG-ARG-56.
RX   PubMed=10664464; DOI=10.1016/s0014-5793(00)01129-7;
RA   Hynds P.J., Pluecken H., Westhoff P., Robinson C.;
RT   "Different lumen-targeting pathways for nuclear-encoded versus
RT   cyanobacterial/plastid-encoded Hcf136 proteins.";
RL   FEBS Lett. 467:97-100(2000).
RN   [9]
RP   FUNCTION IN EARLY ASSEMBLY OF PHOTOSYSTEM II.
RX   PubMed=12459468; DOI=10.1016/s0014-5793(02)03634-7;
RA   Pluecken H., Mueller B., Grohmann D., Westhoff P., Eichacker L.A.;
RT   "The HCF136 protein is essential for assembly of the photosystem II
RT   reaction center in Arabidopsis thaliana.";
RL   FEBS Lett. 532:85-90(2002).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS].
RX   PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA   Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA   van Wijk K.J.;
RT   "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT   proteome.";
RL   PLoS ONE 3:E1994-E1994(2008).
RN   [11]
RP   INTERACTION WITH PAM68.
RX   PubMed=20923938; DOI=10.1105/tpc.110.077453;
RA   Armbruster U., Zuhlke J., Rengstl B., Kreller R., Makarenko E., Ruhle T.,
RA   Schunemann D., Jahns P., Weisshaar B., Nickelsen J., Leister D.;
RT   "The Arabidopsis thylakoid protein PAM68 is required for efficient D1
RT   biogenesis and photosystem II assembly.";
RL   Plant Cell 22:3439-3460(2010).
CC   -!- FUNCTION: Essential for photosystem II (PSII) biogenesis; required for
CC       assembly of an early intermediate in PSII assembly that includes D2
CC       (psbD) and cytochrome b559. Has been suggested (PubMed:11826309) to be
CC       required for chlorophyll a binding. {ECO:0000269|PubMed:11826309,
CC       ECO:0000269|PubMed:12459468, ECO:0000269|PubMed:9736608}.
CC   -!- SUBUNIT: Interacts with PAM68. {ECO:0000269|PubMed:20923938}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC       {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:18431481,
CC       ECO:0000269|PubMed:9736608}. Note=Restricted to the stromal lamellae
CC       (PubMed:9736608). Translocation into the thylakoid lumen occurs via the
CC       Tat pathway (Probable) (PubMed:10664464). {ECO:0000269|PubMed:10664464,
CC       ECO:0000269|PubMed:9736608, ECO:0000305|PubMed:11719511,
CC       ECO:0000305|PubMed:11826309}.
CC   -!- TISSUE SPECIFICITY: Expression in green tissue, not roots.
CC   -!- DEVELOPMENTAL STAGE: Accumulates also in dark-grown seedlings.
CC       {ECO:0000269|PubMed:9736608}.
CC   -!- DOMAIN: A 7-bladed beta-propeller torus, about 54 by 55 Angstroms, with
CC       a depth of about 25 Angstroms and a central pore.
CC       {ECO:0000250|UniProtKB:M1VJU3}.
CC   -!- DISRUPTION PHENOTYPE: Seedling lethal when homozygous, has pale
CC       cotyledons but never develops true leaves. On sucrose-supplemented
CC       medium PSII is completely inactive; D1, D2 CP43 and CP47 are present in
CC       very low amounts. {ECO:0000269|PubMed:9736608}.
CC   -!- SIMILARITY: Belongs to the Ycf48 family. {ECO:0000305}.
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DR   EMBL; Y15628; CAA75723.1; -; mRNA.
DR   EMBL; AB006708; BAB09829.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED93124.1; -; Genomic_DNA.
DR   EMBL; AY045691; AAK74049.1; -; mRNA.
DR   EMBL; BT004549; AAO42795.1; -; mRNA.
DR   EMBL; AK221097; BAD94976.1; -; mRNA.
DR   PIR; T51828; T51828.
DR   RefSeq; NP_197703.1; NM_122218.3.
DR   AlphaFoldDB; O82660; -.
DR   SMR; O82660; -.
DR   BioGRID; 17651; 4.
DR   IntAct; O82660; 4.
DR   STRING; 3702.O82660; -.
DR   PaxDb; 3702-AT5G23120-1; -.
DR   ProteomicsDB; 248814; -.
DR   EnsemblPlants; AT5G23120.1; AT5G23120.1; AT5G23120.
DR   GeneID; 832376; -.
DR   Gramene; AT5G23120.1; AT5G23120.1; AT5G23120.
DR   KEGG; ath:AT5G23120; -.
DR   Araport; AT5G23120; -.
DR   TAIR; AT5G23120; HCF136.
DR   eggNOG; KOG3511; Eukaryota.
DR   HOGENOM; CLU_057027_0_0_1; -.
DR   InParanoid; O82660; -.
DR   PhylomeDB; O82660; -.
DR   PRO; PR:O82660; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; O82660; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR   GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR   GO; GO:0009533; C:chloroplast stromal thylakoid; IDA:TAIR.
DR   GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR   GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR   GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; HDA:TAIR.
DR   GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR   GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:0009657; P:plastid organization; IMP:TAIR.
DR   GO; GO:0065003; P:protein-containing complex assembly; IMP:TAIR.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR028203; PSII_CF48-like_dom.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR016705; Ycf48/Hcf136.
DR   PANTHER; PTHR47199; PHOTOSYSTEM II STABILITY/ASSEMBLY FACTOR HCF136, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR47199:SF2; PHOTOSYSTEM II STABILITY_ASSEMBLY FACTOR HCF136, CHLOROPLASTIC; 1.
DR   Pfam; PF14870; PSII_BNR; 1.
DR   PIRSF; PIRSF017875; PSII_HCF136; 1.
DR   SUPFAM; SSF110296; Oligoxyloglucan reducing end-specific cellobiohydrolase; 1.
DR   World-2DPAGE; 0003:O82660; -.
DR   Genevisible; O82660; AT.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Photosynthesis; Photosystem II;
KW   Plastid; Reference proteome; Thylakoid; Transit peptide.
FT   TRANSIT         1..53
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   TRANSIT         54..78
FT                   /note="Thylakoid"
FT                   /evidence="ECO:0000269|PubMed:11719511,
FT                   ECO:0000269|PubMed:11826309"
FT   CHAIN           79..403
FT                   /note="Photosystem II stability/assembly factor HCF136,
FT                   chloroplastic"
FT                   /id="PRO_0000005341"
FT   REGION          31..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         55..56
FT                   /note="RR->KK: Protein is imported into chloroplasts but is
FT                   unable to translocate into the thylakoid lumen."
FT                   /evidence="ECO:0000269|PubMed:10664464"
SQ   SEQUENCE   403 AA;  44104 MW;  11079552F817FF9D CRC64;
     MASLQLCDGY LLFKPSVSPR FLSQRISHRL IPKASSSPPP SPSPSSSSSS LSFSRRELLY
     QSAAVSLSLS SIVGPARADE QLSEWERVFL PIDPGVVLLD IAFVPDEPSR GFLLGTRQTL
     LETKDGGSTW NPRSIPSAEE EDFNYRFNSI SFKGKEGWII GKPAILLYTA DAGENWDRIP
     LSSQLPGDMV FIKATEDKSA EMVTDEGAIY VTSNRGYNWK AAIQETVSAT LNRTVSSGIS
     GASYYTGTFS AVNRSPDGRY VAVSSRGNFF LTWEPGQPYW QPHNRAVARR IQNMGWRADG
     GLWLLVRGGG LYLSKGTGIT EEFEEVPVQS RGFGILDVGY RSEEEAWAAG GSGILLRTRN
     GGKSWNRDKA ADNIAANLYA VKFVDDKKGF VLGNDGVLLR YVG
//