Reviewed, UniProtKB/Swiss-Prot O82660 (P2SAF_ARATH)

Names and origin

Protein names

Recommended name:
    Photosystem II stability/assembly factor HCF136, chloroplastic

Gene names

Name:	HCF136
Ordered Locus Names:	At5g23120
ORF Names:	MYJ24.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for photosystem II (PSII) biogenesis; required for assembly of an early intermediate in PSII assembly that includes D2 (psbD) and cytochrome b559. Has been suggested (Ref.5) to be required for chlorophyll a binding. Ref.1 Ref.7
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side. Note: Restricted to the stromal lamelae. Translocation into the thylakoid lumen occurs via the Tat pathway. Ref.1
Tissue specificity	Expression in green tissue, not roots.
Developmental stage	Accumulates also in dark-grown seedlings.
Sequence similarities	Belongs to the ycf48 family. Contains 4 BNR repeats.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Domain	Repeat Transit peptide
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW plastid organization Ref.1 Inferred from mutant phenotype. Source: TAIR protein complex assembly Ref.7 Inferred from mutant phenotype. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast stromal thylakoid Ref.1 Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR photosystem II Inferred from electronic annotation. Source: UniProtKB-KW thylakoid lumen Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.7 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Chloroplast Potential
	Transit peptide	54 – 78	25	Thylakoid
	Chain	79 – 403	325	Photosystem II stability/assembly factor HCF136, chloroplastic		PRO_0000005341
Regions
	Repeat	121 – 132	12	BNR 1
	Repeat	167 – 178	12	BNR 2
	Repeat	210 – 221	12	BNR 3
	Repeat	356 – 367	12	BNR 4
Experimental info
	Mutagenesis	55 – 56	2	RR → KK: Protein is imported into chloroplasts but is unable to translocate into the thylakoid lumen. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O82660-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 11079552F817FF9D	FASTA	403	44,104
	10 20 30 40 50 60 MASLQLCDGY LLFKPSVSPR FLSQRISHRL IPKASSSPPP SPSPSSSSSS LSFSRRELLY 70 80 90 100 110 120 QSAAVSLSLS SIVGPARADE QLSEWERVFL PIDPGVVLLD IAFVPDEPSR GFLLGTRQTL 130 140 150 160 170 180 LETKDGGSTW NPRSIPSAEE EDFNYRFNSI SFKGKEGWII GKPAILLYTA DAGENWDRIP 190 200 210 220 230 240 LSSQLPGDMV FIKATEDKSA EMVTDEGAIY VTSNRGYNWK AAIQETVSAT LNRTVSSGIS 250 260 270 280 290 300 GASYYTGTFS AVNRSPDGRY VAVSSRGNFF LTWEPGQPYW QPHNRAVARR IQNMGWRADG 310 320 330 340 350 360 GLWLLVRGGG LYLSKGTGIT EEFEEVPVQS RGFGILDVGY RSEEEAWAAG GSGILLRTRN 370 380 390 400 GGKSWNRDKA ADNIAANLYA VKFVDDKKGF VLGNDGVLLR YVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana." Meurer J., Pluecken H., Kowallik K.V., Westhoff P. EMBO J. 17:5286-5297(1998) [PubMed: 9736608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: cv. Wassilewskija. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-403. Strain: cv. Columbia.
[5]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Different lumen-targeting pathways for nuclear-encoded versus cyanobacterial/plastid-encoded Hcf136 proteins." Hynds P.J., Pluecken H., Westhoff P., Robinson C. FEBS Lett. 467:97-100(2000) [PubMed: 10664464] [Abstract] Cited for: IN VITRO IMPORT INTO PEA CHLOROPLASTS, MUTAGENESIS OF 55-ARG-ARG-56.
[7]	"The HCF136 protein is essential for assembly of the photosystem II reaction center in Arabidopsis thaliana." Pluecken H., Mueller B., Grohmann D., Westhoff P., Eichacker L.A. FEBS Lett. 532:85-90(2002) [PubMed: 12459468] [Abstract] Cited for: FUNCTION IN EARLY ASSEMBLY OF PHOTOSYSTEM II.
[8]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y15628 mRNA. Translation: CAA75723.1. AB006708 Genomic DNA. Translation: BAB09829.1. AY045691 mRNA. Translation: AAK74049.1. BT004549 mRNA. Translation: AAO42795.1. AK221097 mRNA. Translation: BAD94976.1.
IPI	IPI00539527.
PIR	T51828.
RefSeq	NP_197703.1.
UniGene	At.5360
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O82660.
ProMEX	O82660.
Genome annotation databases
GeneID	832376.
GenomeReviews	Gene locus AT5G23120 in contig BA000015_GR.
KEGG	ath:AT5G23120.
NMPDR	fig|3702.1.peg.24483.
Organism-specific databases
TAIR	At5g23120.
Phylogenomic databases
OMA	O82660. GFLVGSN.
Gene expression databases
ArrayExpress	O82660.
GermOnline	AT5G23120. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016705. PSII_stabil/assmbl_fac_HCF136. IPR006311. Tat. [Graphical view]
PIRSF	PIRSF017875. PSII_HCF136. 1 hit.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
ProtoNet	Search...

Entry information

Entry name

P2SAF_ARATH

Accession

Primary (citable) accession number: O82660
Secondary accession number(s): Q56Z72

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)