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O82660 (P2SAF_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photosystem II stability/assembly factor HCF136, chloroplastic
Gene names
Name:HCF136
Ordered Locus Names:At5g23120
ORF Names:MYJ24.11
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for photosystem II (PSII) biogenesis; required for assembly of an early intermediate in PSII assembly that includes D2 (psbD) and cytochrome b559. Has been suggested (Ref.6) to be required for chlorophyll a binding. Ref.1 Ref.8

Subunit structure

Interacts with PAM68. Ref.10

Subcellular location

Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side. Note: Restricted to the stromal lamelae. Translocation into the thylakoid lumen occurs via the Tat pathway. Ref.1

Tissue specificity

Expression in green tissue, not roots.

Developmental stage

Accumulates also in dark-grown seedlings.

Sequence similarities

Belongs to the ycf48 family.

Contains 4 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5353Chloroplast Potential
Transit peptide54 – 7825Thylakoid
Chain79 – 403325Photosystem II stability/assembly factor HCF136, chloroplastic
PRO_0000005341

Regions

Repeat121 – 13212BNR 1
Repeat167 – 17812BNR 2
Repeat210 – 22112BNR 3
Repeat356 – 36712BNR 4

Experimental info

Mutagenesis55 – 562RR → KK: Protein is imported into chloroplasts but is unable to translocate into the thylakoid lumen. Ref.7

Sequences

Sequence LengthMass (Da)Tools
O82660 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 11079552F817FF9D

FASTA40344,104
        10         20         30         40         50         60 
MASLQLCDGY LLFKPSVSPR FLSQRISHRL IPKASSSPPP SPSPSSSSSS LSFSRRELLY 

        70         80         90        100        110        120 
QSAAVSLSLS SIVGPARADE QLSEWERVFL PIDPGVVLLD IAFVPDEPSR GFLLGTRQTL 

       130        140        150        160        170        180 
LETKDGGSTW NPRSIPSAEE EDFNYRFNSI SFKGKEGWII GKPAILLYTA DAGENWDRIP 

       190        200        210        220        230        240 
LSSQLPGDMV FIKATEDKSA EMVTDEGAIY VTSNRGYNWK AAIQETVSAT LNRTVSSGIS 

       250        260        270        280        290        300 
GASYYTGTFS AVNRSPDGRY VAVSSRGNFF LTWEPGQPYW QPHNRAVARR IQNMGWRADG 

       310        320        330        340        350        360 
GLWLLVRGGG LYLSKGTGIT EEFEEVPVQS RGFGILDVGY RSEEEAWAAG GSGILLRTRN 

       370        380        390        400 
GGKSWNRDKA ADNIAANLYA VKFVDDKKGF VLGNDGVLLR YVG

« Hide

References

« Hide 'large scale' references
[1]"A nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana."
Meurer J., Pluecken H., Kowallik K.V., Westhoff P.
EMBO J. 17:5286-5297(1998) [PubMed: 9736608] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: cv. Wassilewskija.
Tissue: Leaf.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-403.
Strain: cv. Columbia.
[6]"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction."
Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J.
Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Different lumen-targeting pathways for nuclear-encoded versus cyanobacterial/plastid-encoded Hcf136 proteins."
Hynds P.J., Pluecken H., Westhoff P., Robinson C.
FEBS Lett. 467:97-100(2000) [PubMed: 10664464] [Abstract]
Cited for: IN VITRO IMPORT INTO PEA CHLOROPLASTS, MUTAGENESIS OF 55-ARG-ARG-56.
[8]"The HCF136 protein is essential for assembly of the photosystem II reaction center in Arabidopsis thaliana."
Pluecken H., Mueller B., Grohmann D., Westhoff P., Eichacker L.A.
FEBS Lett. 532:85-90(2002) [PubMed: 12459468] [Abstract]
Cited for: FUNCTION IN EARLY ASSEMBLY OF PHOTOSYSTEM II.
[9]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[10]"The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly."
Armbruster U., Zuhlke J., Rengstl B., Kreller R., Makarenko E., Ruhle T., Schunemann D., Jahns P., Weisshaar B., Nickelsen J., Leister D.
Plant Cell 22:3439-3460(2010) [PubMed: 20923938] [Abstract]
Cited for: INTERACTION WITH PAM68.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y15628 mRNA. Translation: CAA75723.1.
AB006708 Genomic DNA. Translation: BAB09829.1.
CP002688 Genomic DNA. Translation: AED93124.1.
AY045691 mRNA. Translation: AAK74049.1.
BT004549 mRNA. Translation: AAO42795.1.
AK221097 mRNA. Translation: BAD94976.1.
IPIIPI00539527.
PIRT51828.
RefSeqNP_197703.1. NM_122218.2.
UniGeneAt.5360.

3D structure databases

ProteinModelPortalO82660.
SMRO82660. Positions 82-403.
ModBaseSearch...

Protein-protein interaction databases

IntActO82660. 4 interactions.
STRINGO82660.

2D gel databases

World-2DPAGE0003:O82660.

Proteomic databases

PRIDEO82660.
ProMEXO82660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G23120.1; AT5G23120.1; AT5G23120.
GeneID832376.
GenomeReviewsGene locus AT5G23120 in contig BA000015_GR.
KEGGath:AT5G23120.
NMPDRfig|3702.1.peg.24483.

Organism-specific databases

TAIRAt5g23120.

Phylogenomic databases

eggNOGKOG3511.
GeneTreeEPGT00050000012421.
HOGENOMHBG285032.
InParanoidO82660.
OMAGFLVGSN.
PhylomeDBO82660.
ProtClustDBPLN00033.

Gene expression databases

ArrayExpressO82660.
GenevestigatorO82660.
GermOnlineAT5G23120. Arabidopsis thaliana.

Family and domain databases

InterProIPR002860. BNR_rpt.
IPR016705. PSII_HCF136_stabil/assmbl_fac.
[Graphical view]
PfamPF02012. BNR. 4 hits.
[Graphical view]
PIRSFPIRSF017875. PSII_HCF136. 1 hit.
ProtoNetSearch...

Entry information

Entry nameP2SAF_ARATH
AccessionPrimary (citable) accession number: O82660
Secondary accession number(s): Q56Z72
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents