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Protein

Protein-tyrosine-phosphatase PTP1

Gene

PTP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein-tyrosine-phosphatase that dephosphorylates and probably inhibits MPK6 in non-oxidative stress conditions. In association with MKP1, represses salicylic acid (SA) and camalexin biosynthesis, thus modulating defense response. May also repress MPK3. Dephosphorylates and inactivates MPK4 in vitro.4 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by hydrogen peroxide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234SubstrateCurated1
Active sitei265Phosphocysteine intermediate1
Binding sitei311SubstrateBy similarity1

GO - Molecular functioni

  • MAP kinase tyrosine phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: TAIR

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • intracellular signal transduction Source: TAIR
  • negative regulation of defense response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine-phosphatase PTP1 (EC:3.1.3.48)
Alternative name(s):
Protein tyrosine phosphatase 1
Short name:
AtPTP1
Gene namesi
Name:PTP1
Ordered Locus Names:At1g71860
ORF Names:F14O23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G71860.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234D → A: Loss of activity. 1 Publication1
Mutagenesisi265C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173291 – 340Protein-tyrosine-phosphatase PTP1Add BLAST340

Proteomic databases

PaxDbiO82656.

PTM databases

iPTMnetiO82656.

Expressioni

Tissue specificityi

Expressed in roots, stems and flowers, and at low levels in leaves.1 Publication

Inductioni

By salt treatment. Down-regulated by cold.1 Publication

Gene expression databases

ExpressionAtlasiO82656. baseline and differential.
GenevisibleiO82656. AT.

Interactioni

Subunit structurei

Interacts with MPK6.1 Publication

Protein-protein interaction databases

BioGridi28736. 4 interactors.
IntActiO82656. 1 interactor.
MINTiMINT-8390809.
STRINGi3702.AT1G71860.1.

Structurei

3D structure databases

ProteinModelPortaliO82656.
SMRiO82656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 326Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 271Substrate bindingBy similarity7

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
HOGENOMiHOG000243992.
InParanoidiO82656.
OMAiWEMVIQQ.
OrthoDBiEOG09360FV0.
PhylomeDBiO82656.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82656-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGKTSSAA NLFTGSTRFD LSSADSPPSK LSLSSDQLNH CHQALGVFRG
60 70 80 90 100
KIQNPDSIAH EFTGLQANRM WPSELLLNST VAMNSVNVEK NRYSDVVPFD
110 120 130 140 150
KNRIVLNPCK DSSAKGYVNA SLIKTSESES ISQFIATQGP LPHTMEDFWE
160 170 180 190 200
MVIQQHCPII VMLTRLVDNN RTVKCGDYFQ DEDGPREFGN ISLTTKWIKT
210 220 230 240 250
TDTSLMLRNL EVNYKETEDQ PMSVLHIQYP EWPDHGVPKD TVAVREILKR
260 270 280 290 300
LYQVPPSLGP IIVHCSAGIG RTGTYCAIHN TIQRILAGDM SALDLAKTVA
310 320 330 340
LFRKQRIGMV QTMDQYFFCY NAIVDELEDL TAGTNAGTSS
Length:340
Mass (Da):37,799
Last modified:November 1, 1998 - v1
Checksum:i7885C03A664CA02D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147D → A in AAC68859 (PubMed:9596642).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055635 mRNA. Translation: AAC68859.1.
AJ006309 Genomic DNA. Translation: CAA06978.1.
AC012654 Genomic DNA. Translation: AAF43239.1.
CP002684 Genomic DNA. Translation: AEE35242.1.
CP002684 Genomic DNA. Translation: AEE35243.1.
AY070403 mRNA. Translation: AAL49899.1.
AY117240 mRNA. Translation: AAM51315.1.
PIRiC96741.
T51846.
RefSeqiNP_001031266.1. NM_001036189.1. [O82656-1]
NP_177331.1. NM_105844.4. [O82656-1]
UniGeneiAt.407.

Genome annotation databases

EnsemblPlantsiAT1G71860.1; AT1G71860.1; AT1G71860. [O82656-1]
AT1G71860.3; AT1G71860.3; AT1G71860. [O82656-1]
GeneIDi843516.
GrameneiAT1G71860.1; AT1G71860.1; AT1G71860.
AT1G71860.3; AT1G71860.3; AT1G71860.
KEGGiath:AT1G71860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055635 mRNA. Translation: AAC68859.1.
AJ006309 Genomic DNA. Translation: CAA06978.1.
AC012654 Genomic DNA. Translation: AAF43239.1.
CP002684 Genomic DNA. Translation: AEE35242.1.
CP002684 Genomic DNA. Translation: AEE35243.1.
AY070403 mRNA. Translation: AAL49899.1.
AY117240 mRNA. Translation: AAM51315.1.
PIRiC96741.
T51846.
RefSeqiNP_001031266.1. NM_001036189.1. [O82656-1]
NP_177331.1. NM_105844.4. [O82656-1]
UniGeneiAt.407.

3D structure databases

ProteinModelPortaliO82656.
SMRiO82656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28736. 4 interactors.
IntActiO82656. 1 interactor.
MINTiMINT-8390809.
STRINGi3702.AT1G71860.1.

PTM databases

iPTMnetiO82656.

Proteomic databases

PaxDbiO82656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G71860.1; AT1G71860.1; AT1G71860. [O82656-1]
AT1G71860.3; AT1G71860.3; AT1G71860. [O82656-1]
GeneIDi843516.
GrameneiAT1G71860.1; AT1G71860.1; AT1G71860.
AT1G71860.3; AT1G71860.3; AT1G71860.
KEGGiath:AT1G71860.

Organism-specific databases

TAIRiAT1G71860.

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
HOGENOMiHOG000243992.
InParanoidiO82656.
OMAiWEMVIQQ.
OrthoDBiEOG09360FV0.
PhylomeDBiO82656.

Miscellaneous databases

PROiO82656.

Gene expression databases

ExpressionAtlasiO82656. baseline and differential.
GenevisibleiO82656. AT.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTP1_ARATH
AccessioniPrimary (citable) accession number: O82656
Secondary accession number(s): O65190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.