Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-tyrosine-phosphatase PTP1

Gene

PTP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein-tyrosine-phosphatase that dephosphorylates and probably inhibits MPK6 in non-oxidative stress conditions. In association with MKP1, represses salicylic acid (SA) and camalexin biosynthesis, thus modulating defense response. May also repress MPK3. Dephosphorylates and inactivates MPK4 in vitro.4 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by hydrogen peroxide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei234SubstrateCurated1
Active sitei265Phosphocysteine intermediate1
Binding sitei311SubstrateBy similarity1

GO - Molecular functioni

  • MAP kinase tyrosine phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: TAIR

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • intracellular signal transduction Source: TAIR
  • negative regulation of defense response Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processPlant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine-phosphatase PTP1 (EC:3.1.3.48)
Alternative name(s):
Protein tyrosine phosphatase 1
Short name:
AtPTP1
Gene namesi
Name:PTP1
Ordered Locus Names:At1g71860
ORF Names:F14O23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G71860
TAIRilocus:2012991 AT1G71860

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234D → A: Loss of activity. 1 Publication1
Mutagenesisi265C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173291 – 340Protein-tyrosine-phosphatase PTP1Add BLAST340

Proteomic databases

PaxDbiO82656
PRIDEiO82656

PTM databases

iPTMnetiO82656

Expressioni

Tissue specificityi

Expressed in roots, stems and flowers, and at low levels in leaves.1 Publication

Inductioni

By salt treatment. Down-regulated by cold.1 Publication

Gene expression databases

ExpressionAtlasiO82656 baseline and differential
GenevisibleiO82656 AT

Interactioni

Subunit structurei

Interacts with MPK6.1 Publication

Protein-protein interaction databases

BioGridi28736, 4 interactors
IntActiO82656, 1 interactor
MINTiO82656
STRINGi3702.AT1G71860.1

Structurei

3D structure databases

ProteinModelPortaliO82656
SMRiO82656
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 326Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 271Substrate bindingBy similarity7

Phylogenomic databases

eggNOGiKOG0789 Eukaryota
COG5599 LUCA
HOGENOMiHOG000243992
InParanoidiO82656
OMAiTFRSQRI
OrthoDBiEOG09360FV0
PhylomeDBiO82656

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82656-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGKTSSAA NLFTGSTRFD LSSADSPPSK LSLSSDQLNH CHQALGVFRG
60 70 80 90 100
KIQNPDSIAH EFTGLQANRM WPSELLLNST VAMNSVNVEK NRYSDVVPFD
110 120 130 140 150
KNRIVLNPCK DSSAKGYVNA SLIKTSESES ISQFIATQGP LPHTMEDFWE
160 170 180 190 200
MVIQQHCPII VMLTRLVDNN RTVKCGDYFQ DEDGPREFGN ISLTTKWIKT
210 220 230 240 250
TDTSLMLRNL EVNYKETEDQ PMSVLHIQYP EWPDHGVPKD TVAVREILKR
260 270 280 290 300
LYQVPPSLGP IIVHCSAGIG RTGTYCAIHN TIQRILAGDM SALDLAKTVA
310 320 330 340
LFRKQRIGMV QTMDQYFFCY NAIVDELEDL TAGTNAGTSS
Length:340
Mass (Da):37,799
Last modified:November 1, 1998 - v1
Checksum:i7885C03A664CA02D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147D → A in AAC68859 (PubMed:9596642).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055635 mRNA Translation: AAC68859.1
AJ006309 Genomic DNA Translation: CAA06978.1
AC012654 Genomic DNA Translation: AAF43239.1
CP002684 Genomic DNA Translation: AEE35242.1
CP002684 Genomic DNA Translation: AEE35243.1
AY070403 mRNA Translation: AAL49899.1
AY117240 mRNA Translation: AAM51315.1
PIRiC96741
T51846
RefSeqiNP_001031266.1, NM_001036189.1 [O82656-1]
NP_177331.1, NM_105844.4 [O82656-1]
UniGeneiAt.407

Genome annotation databases

EnsemblPlantsiAT1G71860.1; AT1G71860.1; AT1G71860 [O82656-1]
AT1G71860.3; AT1G71860.3; AT1G71860 [O82656-1]
GeneIDi843516
GrameneiAT1G71860.1; AT1G71860.1; AT1G71860 [O82656-1]
AT1G71860.3; AT1G71860.3; AT1G71860 [O82656-1]
KEGGiath:AT1G71860

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTP1_ARATH
AccessioniPrimary (citable) accession number: O82656
Secondary accession number(s): O65190
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health