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O82656 (PTP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-tyrosine-phosphatase PTP1

EC=3.1.3.48
Alternative name(s):
Protein tyrosine phosphatase 1
Short name=AtPTP1
Gene names
Name:PTP1
Ordered Locus Names:At1g71860
ORF Names:F14O23.24
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein-tyrosine-phosphatase that dephosphorylates and probably inhibits MPK6 in non-oxidative stress conditions. In association with MKP1, represses salicylic acid (SA) and camalexin biosynthesis, thus modulating defense response. May also repress MPK3. Dephosphorylates and inactivates MPK4 in vitro. Ref.1 Ref.6 Ref.7 Ref.8

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulation

Inhibited by hydrogen peroxide. Ref.7

Subunit structure

Interacts with MPK6. Ref.8

Subcellular location

Cytoplasmcytosol. Nucleus Ref.8.

Tissue specificity

Expressed in roots, stems and flowers, and at low levels in leaves. Ref.1

Induction

By salt treatment. Down-regulated by cold. Ref.1 Ref.7

Disruption phenotype

No visible phenotype under normal growth conditions. Ref.8

Sequence similarities

Contains 1 tyrosine-protein phosphatase domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82656-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Protein-tyrosine-phosphatase PTP1
PRO_0000417329

Regions

Domain58 – 326269Tyrosine-protein phosphatase
Region265 – 2717Substrate binding By similarity

Sites

Active site2651Phosphocysteine intermediate
Binding site2341Substrate Probable
Binding site3111Substrate By similarity

Experimental info

Mutagenesis2341D → A: Loss of activity. Ref.1
Mutagenesis2651C → S: Loss of activity. Ref.1
Sequence conflict1471D → A in AAC68859. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7885C03A664CA02D

FASTA34037,799
        10         20         30         40         50         60 
MATGKTSSAA NLFTGSTRFD LSSADSPPSK LSLSSDQLNH CHQALGVFRG KIQNPDSIAH 

        70         80         90        100        110        120 
EFTGLQANRM WPSELLLNST VAMNSVNVEK NRYSDVVPFD KNRIVLNPCK DSSAKGYVNA 

       130        140        150        160        170        180 
SLIKTSESES ISQFIATQGP LPHTMEDFWE MVIQQHCPII VMLTRLVDNN RTVKCGDYFQ 

       190        200        210        220        230        240 
DEDGPREFGN ISLTTKWIKT TDTSLMLRNL EVNYKETEDQ PMSVLHIQYP EWPDHGVPKD 

       250        260        270        280        290        300 
TVAVREILKR LYQVPPSLGP IIVHCSAGIG RTGTYCAIHN TIQRILAGDM SALDLAKTVA 

       310        320        330        340 
LFRKQRIGMV QTMDQYFFCY NAIVDELEDL TAGTNAGTSS 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of a tyrosine-specific protein phosphatase encoded by a stress-responsive gene in Arabidopsis."
Xu Q., Fu H.H., Gupta R., Luan S.
Plant Cell 10:849-857(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF ASP-234 AND CYS-265.
[2]"Higher plant tyrosine-specific protein phosphatases (PTPs) contain novel amino-terminal domains: expression during embryogenesis."
Fordham-Skelton A.P., Skipsey M., Eveans I.M., Edwards R., Gatehouse J.A.
Plant Mol. Biol. 39:593-605(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"ATMPK4, an Arabidopsis homolog of mitogen-activated protein kinase, is activated in vitro by AtMEK1 through threonine phosphorylation."
Huang Y., Li H., Gupta R., Morris P.C., Luan S., Kieber J.J.
Plant Physiol. 122:1301-1310(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Redox control of protein tyrosine phosphatases and mitogen-activated protein kinases in plants."
Gupta R., Luan S.
Plant Physiol. 132:1149-1152(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
[8]"MAP kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and SNC1-mediated responses in Arabidopsis."
Bartels S., Anderson J.C., Gonzalez Besteiro M.A., Carreri A., Hirt H., Buchala A., Metraux J.P., Peck S.C., Ulm R.
Plant Cell 21:2884-2897(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MPK6, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF055635 mRNA. Translation: AAC68859.1.
AJ006309 Genomic DNA. Translation: CAA06978.1.
AC012654 Genomic DNA. Translation: AAF43239.1.
CP002684 Genomic DNA. Translation: AEE35242.1.
CP002684 Genomic DNA. Translation: AEE35243.1.
AY070403 mRNA. Translation: AAL49899.1.
AY117240 mRNA. Translation: AAM51315.1.
PIRC96741.
T51846.
RefSeqNP_001031266.1. NM_001036189.1. [O82656-1]
NP_177331.1. NM_105844.3. [O82656-1]
UniGeneAt.407.

3D structure databases

ProteinModelPortalO82656.
SMRO82656. Positions 42-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO82656. 1 interaction.
MINTMINT-8390809.

Proteomic databases

PRIDEO82656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G71860.1; AT1G71860.1; AT1G71860. [O82656-1]
AT1G71860.3; AT1G71860.3; AT1G71860. [O82656-1]
GeneID843516.
KEGGath:AT1G71860.

Organism-specific databases

TAIRAT1G71860.

Phylogenomic databases

HOGENOMHOG000243992.
InParanoidO82656.
OMAIGMVQTM.
PhylomeDBO82656.

Enzyme and pathway databases

BioCycARA:AT1G71860-MONOMER.
ARA:GQT-335-MONOMER.

Gene expression databases

ArrayExpressO82656.
GenevestigatorO82656.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO82656.

Entry information

Entry namePTP1_ARATH
AccessionPrimary (citable) accession number: O82656
Secondary accession number(s): O65190
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names