O82656 (PTP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-tyrosine-phosphatase PTP1
EC=3.1.3.48

Alternative name(s):
Protein tyrosine phosphatase 1
Short name=AtPTP1

Gene names

Name:	PTP1
Ordered Locus Names:	At1g71860
ORF Names:	F14O23.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein-tyrosine-phosphatase that dephosphorylates and probably inhibits MPK6 in non-oxidative stress conditions. In association with MKP1, represses salicylic acid (SA) and camalexin biosynthesis, thus modulating defense response. May also repress MPK3. Dephosphorylates and inactivates MPK4 in vitro. Ref.1 Ref.6 Ref.7 Ref.8
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Enzyme regulation	Inhibited by hydrogen peroxide. Ref.7
Subunit structure	Interacts with MPK6. Ref.8
Subcellular location	Cytoplasm › cytosol. Nucleus Ref.8.
Tissue specificity	Expressed in roots, stems and flowers, and at low levels in leaves. Ref.1
Induction	By salt treatment. Down-regulated by cold. Ref.1 Ref.7
Disruption phenotype	No visible phenotype under normal growth conditions. Ref.8
Sequence similarities	Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein kinase cascade Traceable author statement Ref.1. Source: TAIR negative regulation of defense response Inferred from genetic interaction Ref.8. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.8. Source: UniProtKB nucleus Inferred from direct assay Ref.8. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 14506206PubMed 17317660PubMed 17644812. Source: TAIR
Molecular_function	MAP kinase tyrosine phosphatase activity Inferred from direct assay Ref.6Ref.8. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O82656-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Protein-tyrosine-phosphatase PTP1		PRO_0000417329
Regions
	Domain	58 – 326	269	Tyrosine-protein phosphatase
	Region	265 – 271	7	Substrate binding By similarity
Sites
	Active site	265	1	Phosphocysteine intermediate
	Binding site	234	1	Substrate Probable
	Binding site	311	1	Substrate By similarity
Experimental info
	Mutagenesis	234	1	D → A: Loss of activity. Ref.1
	Mutagenesis	265	1	C → S: Loss of activity. Ref.1
	Sequence conflict	147	1	D → A in AAC68859. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 7885C03A664CA02D	FASTA	340	37,799
	10 20 30 40 50 60 MATGKTSSAA NLFTGSTRFD LSSADSPPSK LSLSSDQLNH CHQALGVFRG KIQNPDSIAH 70 80 90 100 110 120 EFTGLQANRM WPSELLLNST VAMNSVNVEK NRYSDVVPFD KNRIVLNPCK DSSAKGYVNA 130 140 150 160 170 180 SLIKTSESES ISQFIATQGP LPHTMEDFWE MVIQQHCPII VMLTRLVDNN RTVKCGDYFQ 190 200 210 220 230 240 DEDGPREFGN ISLTTKWIKT TDTSLMLRNL EVNYKETEDQ PMSVLHIQYP EWPDHGVPKD 250 260 270 280 290 300 TVAVREILKR LYQVPPSLGP IIVHCSAGIG RTGTYCAIHN TIQRILAGDM SALDLAKTVA 310 320 330 340 LFRKQRIGMV QTMDQYFFCY NAIVDELEDL TAGTNAGTSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a tyrosine-specific protein phosphatase encoded by a stress-responsive gene in Arabidopsis." Xu Q., Fu H.H., Gupta R., Luan S. Plant Cell 10:849-857(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, MUTAGENESIS OF ASP-234 AND CYS-265.
[2]	"Higher plant tyrosine-specific protein phosphatases (PTPs) contain novel amino-terminal domains: expression during embryogenesis." Fordham-Skelton A.P., Skipsey M., Eveans I.M., Edwards R., Gatehouse J.A. Plant Mol. Biol. 39:593-605(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"ATMPK4, an Arabidopsis homolog of mitogen-activated protein kinase, is activated in vitro by AtMEK1 through threonine phosphorylation." Huang Y., Li H., Gupta R., Morris P.C., Luan S., Kieber J.J. Plant Physiol. 122:1301-1310(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Redox control of protein tyrosine phosphatases and mitogen-activated protein kinases in plants." Gupta R., Luan S. Plant Physiol. 132:1149-1152(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION.
[8]	"MAP kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and SNC1-mediated responses in Arabidopsis." Bartels S., Anderson J.C., Gonzalez Besteiro M.A., Carreri A., Hirt H., Buchala A., Metraux J.P., Peck S.C., Ulm R. Plant Cell 21:2884-2897(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPK6, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF055635 mRNA. Translation: AAC68859.1. AJ006309 Genomic DNA. Translation: CAA06978.1. AC012654 Genomic DNA. Translation: AAF43239.1. CP002684 Genomic DNA. Translation: AEE35242.1. CP002684 Genomic DNA. Translation: AEE35243.1. AY070403 mRNA. Translation: AAL49899.1. AY117240 mRNA. Translation: AAM51315.1.
IPI	IPI00542999.
PIR	C96741. T51846.
RefSeq	NP_001031266.1. NM_001036189.1. NP_177331.1. NM_105844.3.
UniGene	At.407.
3D structure databases
HSSP	HSSP built from PDB template 1GWZ based on UniProtKB P29350.
ProteinModelPortal	O82656.
SMR	O82656. Positions 61-330.
ModBase	Search...
Proteomic databases
PRIDE	O82656.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G71860.1; AT1G71860.1; AT1G71860. AT1G71860.3; AT1G71860.3; AT1G71860.
GeneID	843516.
KEGG	ath:AT1G71860.
Organism-specific databases
TAIR	At1g71860.
Phylogenomic databases
HOGENOM	HOG000243992.
InParanoid	O82656.
OMA	QRIGMVQ.
PhylomeDB	O82656.
ProtClustDB	CLSN2679290.
Gene expression databases
ArrayExpress	O82656.
Genevestigator	O82656.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTP1_ARATH

Accession

Primary (citable) accession number: O82656
Secondary accession number(s): O65190

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program