O82627 (SSG1_ANTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granule-bound starch synthase 1, chloroplastic/amyloplastic
EC=2.4.1.21

Alternative name(s):
Granule-bound starch synthase I
Short name=GBSS-I

Gene names

Name:	WAXY
Synonyms:	GBSS

Organism

Antirrhinum majus (Garden snapdragon)

Taxonomic identifier

4151 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Plantaginaceae › Antirrhineae › Antirrhinum

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).
Pathway	Glycan biosynthesis; starch biosynthesis.
Subcellular location	Plastid › chloroplast. Plastid › amyloplast. Note: Amyloplast or chloroplast, granule-bound.
Tissue specificity	In leaves, flowers and fruits. Observed in all floral whorls at early developmental stages, but restricted to carpel before anthesis.
Induction	Expressed with a circadian rhythm with peak expression at the end of the day.
Sequence similarities	Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

Ontologies

Keywords
Biological process	Starch biosynthesis
Cellular component	Amyloplast Chloroplast Plastid
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	starch biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	amyloplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	starch synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 78	78	Chloroplast By similarity
	Chain	79 – 608	530	Granule-bound starch synthase 1, chloroplastic/amyloplastic		PRO_0000011124
Sites
	Binding site	96	1	ADP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82627 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 6527D53D565B6E0C	FASTA	608	66,361
	10 20 30 40 50 60 MATVTASQLV SHVHGGATSS PDTKTNLAQV GLRNQQFTHN GLRSINMVDK LQMRNNAKQS 70 80 90 100 110 120 RSLVKKTDNG SPLGKIICGT GMNLVFVLAE VGPWSKTGGL GDVVGGLPPA MAGNGHRVMT 130 140 150 160 170 180 VSPRYDQYKD AWDTSVVVEI KVGDSIETVR FFHCYKRGVD RVFVDHPIFL EKVWGKTKSK 190 200 210 220 230 240 IYGPNAGTDY QDNQLRFSLL CQAALEAPRV LNLTSSKYFS GPYGEDVVFV ANDWHTALLP 250 260 270 280 290 300 CYLKSMYQSK GMYLHAKVAF CIHNIAYQGR FGSSDFCLLN LPDQFKSSFD FFDGYEKPVK 310 320 330 340 350 360 GRKINWMKAG ILESDRVVTV SPYYAMELVS GAEKGVELDN VIAKTSITGI VNGMDTQEWN 370 380 390 400 410 420 PATDKHIDTN YDITTVMDAK PLLKEALQAA VGLPVDKNIP VIGFIGRLEE QKGSDILVAA 430 440 450 460 470 480 ISKFVGLDVQ IIILGTGKKK FEQQIQELEV LYPDKARGVA KFNVPLAHMI TAGADFMLVP 490 500 510 520 530 540 SRFEPCGLIQ LHAMRYGTIP ICASTGGLVD TVTEGFTGFH MGAFNVECAT VDPADVQKIA 550 560 570 580 590 600 TTVERALAAY GSVAYKEMIQ NCMAQDLSWK GPAKNWEKML LSLGVSGSEP GVDGEEIAPL AKENVATP

References

[1]

"Expression of the granule-bound starch synthase I (Waxy) gene from snapdragon is developmentally and circadian clock regulated." Merida A., Rodriguez-Galan J.M., Vincent C., Romero J.M. Plant Physiol. 120:401-410(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006293 mRNA. Translation: CAA06958.1. AJ006294 Genomic DNA. Translation: CAA06959.1.
3D structure databases
ProteinModelPortal	O82627.
ModBase	Search...
Protein family/group databases
CAZy	GT5. Glycosyltransferase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00152.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. IPR011835. Glycogen/starch_synth. IPR013534. Starch_synth_cat_dom. [Graphical view]
Pfam	PF08323. Glyco_transf_5. 1 hit. PF00534. Glycos_transf_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02095. glgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SSG1_ANTMA

Accession

Primary (citable) accession number: O82627

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	January 9, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program