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Protein

Choline/ethanolaminephosphotransferase 2

Gene

AAPT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.1 Publication

Catalytic activityi

CDP-ethanolamine + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylethanolamine.1 Publication
CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable ethanolamine kinase (EMB1187)
  2. Ethanolamine-phosphate cytidylyltransferase (PECT1)
  3. Choline/ethanolaminephosphotransferase 2 (AAPT2), Choline/ethanolaminephosphotransferase 1 (AAPT1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase 1 (CCT1), Choline-phosphate cytidylyltransferase 2 (CCT2)
  2. Choline/ethanolaminephosphotransferase 2 (AAPT2), Choline/ethanolaminephosphotransferase 1 (AAPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G25585-MONOMER.
ARA:GQT-983-MONOMER.
BRENDAi2.7.8.1. 399.
ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-1483213. Synthesis of PE.
UniPathwayiUPA00558; UER00743.
UPA00753; UER00740.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline/ethanolaminephosphotransferase 2 (EC:2.7.8.1, EC:2.7.8.2)
Alternative name(s):
Aminoalcohol phosphotransferase 2
Short name:
AtAAPT2
Gene namesi
Name:AAPT2
Ordered Locus Names:At3g25585
ORF Names:MWL2, T5M7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G25585.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Transmembranei286 – 306HelicalSequence analysisAdd BLAST21
Transmembranei321 – 338HelicalSequence analysisAdd BLAST18
Transmembranei350 – 370HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004233451 – 389Choline/ethanolaminephosphotransferase 2Add BLAST389

Proteomic databases

PaxDbiO82568.

Expressioni

Gene expression databases

ExpressionAtlasiO82568. baseline and differential.
GenevisibleiO82568. AT.

Interactioni

Protein-protein interaction databases

BioGridi10843. 3 interactors.
IntActiO82568. 3 interactors.
STRINGi3702.AT3G25585.1.

Structurei

3D structure databases

ProteinModelPortaliO82568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
HOGENOMiHOG000157893.
InParanoidiO82568.
KOiK00993.
OMAiTEVICEF.
OrthoDBiEOG09360CBY.
PhylomeDBiO82568.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82568-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGYIGAHGVA ALKKHKYSGV DHSYLAKYVL QPFWNRFVKI FPLWMPPNMI
60 70 80 90 100
TLMGFMFLLT SALLGYIYSP KLDSPPPRWV HFAHGLLLFL YQTFDAVDGK
110 120 130 140 150
QARRTNSSSP LGELFDHGCD ALGCALETMA YGSTAMCGRD TFWFWVISAV
160 170 180 190 200
PFFGATWEHY FTNTLTLPVV NGPTEGLALI YCGHFFTAIV GAEWWAQPFG
210 220 230 240 250
KSIPLFSWVP FLNEMQMSRI ILFSMIFFAV IPTLAINTSN VYKVVHSRNG
260 270 280 290 300
SMLLALAMLY PLVTLIAGVL IWDYLSPIDL IRNYPHLVVL GTGLAFGFLV
310 320 330 340 350
GRMILAHLCD EPKGLKTNMC MSLLYLPFAL ANALTARLND GVPLVDEFWV
360 370 380
LLGYCIFTLS LYAHFATSVI HEITTALGIY CFRITRKEA
Length:389
Mass (Da):43,639
Last modified:November 1, 1998 - v1
Checksum:i8289E7671D2BAC55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091844 mRNA. Translation: AAC61769.1.
AB025639 Genomic DNA. No translation available.
AP001313 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE77035.1.
CP002686 Genomic DNA. Translation: AEE77036.1.
CP002686 Genomic DNA. Translation: AEE77038.1.
AF360151 mRNA. Translation: AAK25861.1.
AY054269 mRNA. Translation: AAL06928.1.
AY142609 mRNA. Translation: AAN13178.1.
RefSeqiNP_001030767.1. NM_001035690.1. [O82568-1]
NP_189186.1. NM_113456.4. [O82568-1]
NP_850744.1. NM_180413.2. [O82568-1]
UniGeneiAt.25107.

Genome annotation databases

EnsemblPlantsiAT3G25585.1; AT3G25585.1; AT3G25585. [O82568-1]
AT3G25585.2; AT3G25585.2; AT3G25585. [O82568-1]
AT3G25585.4; AT3G25585.4; AT3G25585. [O82568-1]
GeneIDi825529.
GrameneiAT3G25585.1; AT3G25585.1; AT3G25585.
AT3G25585.2; AT3G25585.2; AT3G25585.
AT3G25585.4; AT3G25585.4; AT3G25585.
KEGGiath:AT3G25585.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091844 mRNA. Translation: AAC61769.1.
AB025639 Genomic DNA. No translation available.
AP001313 Genomic DNA. No translation available.
CP002686 Genomic DNA. Translation: AEE77035.1.
CP002686 Genomic DNA. Translation: AEE77036.1.
CP002686 Genomic DNA. Translation: AEE77038.1.
AF360151 mRNA. Translation: AAK25861.1.
AY054269 mRNA. Translation: AAL06928.1.
AY142609 mRNA. Translation: AAN13178.1.
RefSeqiNP_001030767.1. NM_001035690.1. [O82568-1]
NP_189186.1. NM_113456.4. [O82568-1]
NP_850744.1. NM_180413.2. [O82568-1]
UniGeneiAt.25107.

3D structure databases

ProteinModelPortaliO82568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10843. 3 interactors.
IntActiO82568. 3 interactors.
STRINGi3702.AT3G25585.1.

Proteomic databases

PaxDbiO82568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G25585.1; AT3G25585.1; AT3G25585. [O82568-1]
AT3G25585.2; AT3G25585.2; AT3G25585. [O82568-1]
AT3G25585.4; AT3G25585.4; AT3G25585. [O82568-1]
GeneIDi825529.
GrameneiAT3G25585.1; AT3G25585.1; AT3G25585.
AT3G25585.2; AT3G25585.2; AT3G25585.
AT3G25585.4; AT3G25585.4; AT3G25585.
KEGGiath:AT3G25585.

Organism-specific databases

TAIRiAT3G25585.

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
HOGENOMiHOG000157893.
InParanoidiO82568.
KOiK00993.
OMAiTEVICEF.
OrthoDBiEOG09360CBY.
PhylomeDBiO82568.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00743.
UPA00753; UER00740.
BioCyciARA:AT3G25585-MONOMER.
ARA:GQT-983-MONOMER.
BRENDAi2.7.8.1. 399.
ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-1483213. Synthesis of PE.

Miscellaneous databases

PROiO82568.

Gene expression databases

ExpressionAtlasiO82568. baseline and differential.
GenevisibleiO82568. AT.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAPT2_ARATH
AccessioniPrimary (citable) accession number: O82568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.