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Protein

Glutamine synthetase cytosolic isozyme 2

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 2 (EC:6.3.1.2)
Alternative name(s):
GS1-2
Glutamate--ammonia ligase
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase cytosolic isozyme 2PRO_0000153197Add
BLAST

Proteomic databases

PRIDEiO82560.
ProMEXiO82560.

Expressioni

Tissue specificityi

This is a nodule isozyme.

Gene expression databases

GenevestigatoriO82560.

Interactioni

Subunit structurei

Homooctamer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-10192111,EBI-10192111

Structurei

3D structure databases

ProteinModelPortaliO82560.
SMRiO82560. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

InParanoidiO82560.
KOiK01915.
OMAiGGITHIM.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLINL NLSDITDKVI AEYIWVGGSG MDMRSKARTL SGPVKDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGQDSEV ILYPQAIFKD PFRRGSNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRNNA AKIFGHPDVA AEEPWYGLEQ EYTLLQKDVQ WPLGWPLGGF
160 170 180 190 200
PGPQGPYYCG TGANKAFGRD IVDSHYKACI YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSIGISA ADELWVARYI LERITEIAGV VLSFDPKPIQ GDWNGAGAHT
260 270 280 290 300
NYSTKSMRND GGYEVIKKAI AKLEKRHKEH IAAYGEGNER RLTGRHETAD
310 320 330 340 350
MNTFVWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIAET

TILWKP
Length:356
Mass (Da):39,208
Last modified:November 1, 1998 - v1
Checksum:i4B9C63B7718656B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091456 Genomic DNA. Translation: AAC97935.1.
RefSeqiXP_003544980.1. XM_003544932.2.
UniGeneiGma.29858.

Genome annotation databases

EnsemblPlantsiGLYMA14G39420.1; GLYMA14G39420.1; GLYMA14G39420.
GeneIDi547798.
KEGGigmx:547798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091456 Genomic DNA. Translation: AAC97935.1.
RefSeqiXP_003544980.1. XM_003544932.2.
UniGeneiGma.29858.

3D structure databases

ProteinModelPortaliO82560.
SMRiO82560. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO82560.
ProMEXiO82560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA14G39420.1; GLYMA14G39420.1; GLYMA14G39420.
GeneIDi547798.
KEGGigmx:547798.

Phylogenomic databases

InParanoidiO82560.
KOiK01915.
OMAiGGITHIM.

Gene expression databases

GenevestigatoriO82560.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Soybean gene coding for a nodule specific glutamine synthetase."
    Morey K.J., Sengupta-Gopalan C.
    Plant Gene Register PGR98-194
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Resnik.
    Tissue: Root nodule.

Entry informationi

Entry nameiGLNA2_SOYBN
AccessioniPrimary (citable) accession number: O82560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1998
Last modified: May 27, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.