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Protein

Phospholipase D alpha 1

Gene

PLD1

Organism
Brassica oleracea var. capitata (Cabbage)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence.

Miscellaneous

The propeptide appears to play a key role in the proper folding and activation of the enzyme.

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.

Cofactori

Ca2+Note: Ca2+ requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei334PROSITE-ProRule annotation1
Active sitei339PROSITE-ProRule annotation1
Active sitei661PROSITE-ProRule annotation1
Active sitei663PROSITE-ProRule annotation1
Active sitei668PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.4.4. 946.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D alpha 1 (EC:3.1.4.4)
Short name:
PLD 1
Alternative name(s):
Choline phosphatase 1
Phosphatidylcholine-hydrolyzing phospholipase D 1
Gene namesi
Name:PLD1
OrganismiBrassica oleracea var. capitata (Cabbage)
Taxonomic identifieri3716 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000246491 – 36By similarityAdd BLAST36
ChainiPRO_000002465037 – 810Phospholipase D alpha 1Add BLAST774

Proteomic databases

PRIDEiO82549.

Structurei

3D structure databases

ProteinModelPortaliO82549.
SMRiO82549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110C2Add BLAST110
Domaini327 – 366PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST40
Domaini656 – 683PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Domaini

C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR035892. C2_domain_sf.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR024632. PLipase_D_C.
IPR015679. PLipase_D_fam.
IPR011402. PLipase_D_pln.
PANTHERiPTHR18896. PTHR18896. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 1 hit.
PF12357. PLD_C. 1 hit.
PF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
PIRSFiPIRSF036470. PLD_plant. 1 hit.
SMARTiView protein in SMART
SM00239. C2. 1 hit.
SM00155. PLDc. 2 hits.
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiView protein in PROSITE
PS50035. PLD. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQHLLHGTL HATIYEVDDL HTGGLRSGFF GKILANVEET IGVGKGETQL
60 70 80 90 100
YATIDLQRAR VGRTRKIKDE AKNPKWYESF HIYCAHLASD IIFTVKDDNP
110 120 130 140 150
IGATLIGRAY VPVDQVIHGE EVDQWVEILD NDRNPIHGGS KIHVKLQYFG
160 170 180 190 200
VEADRNWNQG IKSAKFPGVP YTFFSQRQGC KVSLYQDAHI PDNFVPRIPL
210 220 230 240 250
AGGKNYEPQR CWEDIFDAIS NAQHMIYITG WSVYTEIALV RDSRRPKPGG
260 270 280 290 300
DVTVGELLKK KASEGVRVLL LVWDDRTSVD VLKKDGLMAT HDEETENFFR
310 320 330 340 350
GSDVHCILCP RNPDDGGSIV QNLQVSAMFT HHQKIVVVDS EMPSRGGSQM
360 370 380 390 400
RRIVSFVGGI DLCDGRYDTP FHSLFRTLDT VHHDDFHQPN FTGAAITKGG
410 420 430 440 450
PREPWHDIHS RLEGPIAWDV LYNFEQRWSK QGGKDILVKL RELSDIIITP
460 470 480 490 500
SPVMFQEDHD VWNVQLFRSI DGGAAAGFPE SPEAAAEAGL VSGKDNIIDR
510 520 530 540 550
SIQDAYIHAI RRAKDFIYIE NQYFLGSSFA WAADGITPED INALHLIPKE
560 570 580 590 600
LSLKIVSKIE KGEKFRVYVV VPMWPEGLPE SASVQAILDW QRRTMQMMYK
610 620 630 640 650
DIVQALRAQG LEEDPRNYLT FFCLGNREVK KEGEYEPAER PDADSSYMKA
660 670 680 690 700
QEARRFMIYV HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPH
710 720 730 740 750
HLSHRQPARG QIHGFRMSLW YEHLGMLDET FLDPSSVECI EKVNRISDKY
760 770 780 790 800
WDLYSSESLE HDLPGHLLRY PVDVDGEGDV TEFPGFEFFP DTKARILGTK
810
SDYLPPILTT
Length:810
Mass (Da):91,838
Last modified:November 1, 1998 - v1
Checksum:iCBC5B3D7E1F0DAAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090445 mRNA. Translation: AAC78487.1.
AF113918 Genomic DNA. Translation: AAD17208.1.
UniGeneiBol.1479.

Similar proteinsi

Entry informationi

Entry nameiPLDA1_BRAOC
AccessioniPrimary (citable) accession number: O82549
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families