Reviewed,
UniProtKB/Swiss-Prot O82549 (PLDA1_BRAOC)
Last modified
October 13, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phospholipase D alpha 1 Short name=PLD 1 EC=3.1.4.4 Alternative name(s): Choline phosphatase 1 Phosphatidylcholine-hydrolyzing phospholipase D 1 | ||
| Gene names |
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| Organism | Brassica oleracea var. capitata (Cabbage) | ||
| Taxonomic identifier | 3716 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica |
Protein attributes
| Sequence length | 810 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence. |
| Catalytic activity | A phosphatidylcholine + H2O = choline + a phosphatidate. |
| Cofactor | Calcium. Calcium requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent). |
| Subcellular location | Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. |
| Domain | C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands. |
| Miscellaneous | The propeptide appears to play a key role in the proper folding and activation of the enzyme. |
| Sequence similarities | Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid degradation |
| Cellular component | Cytoplasm Membrane |
| Domain | Repeat |
| Ligand | Calcium |
| Molecular function | Hydrolase |
| Gene Ontology (GO) | |
| Biological process | lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion bindingInferred from electronic annotation. Source: UniProtKB-KW phospholipase D activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Propeptide | 1 – 36 | 36 | By similarity | PRO_0000024649 | |||||
| Chain | 37 – 810 | 774 | Phospholipase D alpha 1 | PRO_0000024650 | |||||
Regions | |||||||||
| Domain | 1 – 110 | 110 | C2 | ||||||
| Domain | 327 – 366 | 40 | PLD phosphodiesterase 1 | ||||||
| Domain | 656 – 683 | 28 | PLD phosphodiesterase 2 | ||||||
Sites | |||||||||
| Active site | 332 | 1 | Potential | ||||||
| Active site | 334 | 1 | Potential | ||||||
| Active site | 339 | 1 | Potential | ||||||
| Active site | 661 | 1 | Potential | ||||||
| Active site | 663 | 1 | Potential | ||||||
| Active site | 668 | 1 | Potential | ||||||
Sequences
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References
| [1] | "Identification of two isoenzymes of phospholipase D from cabbage (Brassica oleracea var. capitata)." Pannenberg I., Mansfeld J., Ulbrich-Hofmann R. Plant Gene Register PGR98-188 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| AF090445 mRNA. Translation: AAC78487.1. AF113918 Genomic DNA. Translation: AAD17208.1. | |
3D structure databases | |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 3.1.4.4. 289288. |
Family and domain databases | |
| InterPro | IPR000008. C2_Ca-dep. IPR015679. Phospholipase_D. IPR011402. PLD_pln. IPR001736. PLipase_D/transphosphatidylase. [Graphical view] |
| PANTHER | PTHR18896. Phospholipase_D. 1 hit. |
| Pfam | PF00168. C2. 1 hit. PF00614. PLDc. 2 hits. [Graphical view] |
| PIRSF | PIRSF036470. PLD_plant. 1 hit. |
| SMART | SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view] |
| PROSITE | PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PLDA1_BRAOC | ||||||||
| Accession | Primary (citable) accession number: O82549 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


