O82549 (PLDA1_BRAOC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase D alpha 1
Short name=PLD 1
EC=3.1.4.4

Alternative name(s):
Choline phosphatase 1
Phosphatidylcholine-hydrolyzing phospholipase D 1

Gene names

Name:

PLD1

Organism

Brassica oleracea var. capitata (Cabbage)

Taxonomic identifier

3716 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action, vesicular trafficking, secretion, cytoskeletal arrangement, meiosis, tumor promotion, pathogenesis, membrane deterioration and senescence.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Cofactor	Calcium. Calcium requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2-dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Domain	C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
Miscellaneous	The propeptide appears to play a key role in the proper folding and activation of the enzyme.
Sequence similarities	Belongs to the phospholipase D family. C2-PLD subfamily. Contains 1 C2 domain. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	Calcium
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylcholine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 36	36	By similarity		PRO_0000024649
	Chain	37 – 810	774	Phospholipase D alpha 1		PRO_0000024650
Regions
	Domain	1 – 110	110	C2
	Domain	327 – 366	40	PLD phosphodiesterase 1
	Domain	656 – 683	28	PLD phosphodiesterase 2
Sites
	Active site	332	1	Potential
	Active site	334	1	Potential
	Active site	339	1	Potential
	Active site	661	1	Potential
	Active site	663	1	Potential
	Active site	668	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O82549 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CBC5B3D7E1F0DAAB	FASTA	810	91,838
	10 20 30 40 50 60 MAQHLLHGTL HATIYEVDDL HTGGLRSGFF GKILANVEET IGVGKGETQL YATIDLQRAR 70 80 90 100 110 120 VGRTRKIKDE AKNPKWYESF HIYCAHLASD IIFTVKDDNP IGATLIGRAY VPVDQVIHGE 130 140 150 160 170 180 EVDQWVEILD NDRNPIHGGS KIHVKLQYFG VEADRNWNQG IKSAKFPGVP YTFFSQRQGC 190 200 210 220 230 240 KVSLYQDAHI PDNFVPRIPL AGGKNYEPQR CWEDIFDAIS NAQHMIYITG WSVYTEIALV 250 260 270 280 290 300 RDSRRPKPGG DVTVGELLKK KASEGVRVLL LVWDDRTSVD VLKKDGLMAT HDEETENFFR 310 320 330 340 350 360 GSDVHCILCP RNPDDGGSIV QNLQVSAMFT HHQKIVVVDS EMPSRGGSQM RRIVSFVGGI 370 380 390 400 410 420 DLCDGRYDTP FHSLFRTLDT VHHDDFHQPN FTGAAITKGG PREPWHDIHS RLEGPIAWDV 430 440 450 460 470 480 LYNFEQRWSK QGGKDILVKL RELSDIIITP SPVMFQEDHD VWNVQLFRSI DGGAAAGFPE 490 500 510 520 530 540 SPEAAAEAGL VSGKDNIIDR SIQDAYIHAI RRAKDFIYIE NQYFLGSSFA WAADGITPED 550 560 570 580 590 600 INALHLIPKE LSLKIVSKIE KGEKFRVYVV VPMWPEGLPE SASVQAILDW QRRTMQMMYK 610 620 630 640 650 660 DIVQALRAQG LEEDPRNYLT FFCLGNREVK KEGEYEPAER PDADSSYMKA QEARRFMIYV 670 680 690 700 710 720 HTKMMIVDDE YIIIGSANIN QRSMDGARDS EIAMGGYQPH HLSHRQPARG QIHGFRMSLW 730 740 750 760 770 780 YEHLGMLDET FLDPSSVECI EKVNRISDKY WDLYSSESLE HDLPGHLLRY PVDVDGEGDV 790 800 810 TEFPGFEFFP DTKARILGTK SDYLPPILTT

References

[1]	"Identification of two isoenzymes of phospholipase D from cabbage (Brassica oleracea var. capitata)." Pannenberg I., Mansfeld J., Ulbrich-Hofmann R. Plant Gene Register PGR98-188 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF090445 mRNA. Translation: AAC78487.1. AF113918 Genomic DNA. Translation: AAD17208.1.
3D structure databases
ProteinModelPortal	O82549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR015679. PLipase_D. IPR001736. PLipase_D/transphosphatidylase. IPR024632. PLipase_D_C. IPR011402. PLipase_D_pln. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00168. C2. 1 hit. PF12357. PLD_C. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF036470. PLD_plant. 1 hit.
SMART	SM00239. C2. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. False negative. PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDA1_BRAOC

Accession

Primary (citable) accession number: O82549

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program