Reviewed, UniProtKB/Swiss-Prot O82530 (PSA4_PETHY)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-4
    EC=3.4.25.1
Alternative name(s):
    20S proteasome alpha subunit C
    20S proteasome subunit alpha-3

Gene names

Name:	PAC1
Synonyms:	PRS1

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Proteasome subunit alpha type-4		PRO_0000124113

Sequences

	Sequence		Length	Mass (Da)
	O82530-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 662E72C4945F11A5	FASTA	249	27,232
	10 20 30 40 50 60 MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGN AGSAIGISSK DGVVLVGEKK VTSKLLQTST 70 80 90 100 110 120 SSEKMYKIDD HVACAVAGIM SDANILINTA RVQAQRYTFS YQEPMPVEQL VQSLCDTKQG 130 140 150 160 170 180 YTQYGGLPPF GVSFLFAGWD KNFGFQLFMS DPSGNYAGWK AAAIGANNQA AQSMLKQDYK 190 200 210 220 230 240 DDITREEAVQ LALKVLSKTM DSTSLTSEKL ELAEVFLSNG KVKYQACSPE KLNSMLVKSG LTQPSAEES

References

[1]	Lee H.S., Moon J.H., Kim S.G. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Petal.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF088914 mRNA. Translation: AAC35982.1.
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P23638.
ModBase	Search...
Protein family/group databases
MEROPS	T01.973.
Enzyme and pathway databases
BRENDA	3.4.25.1. 2263.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA4_PETHY

Accession

Primary (citable) accession number: O82530

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1998
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)