O82515 (MTDH_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannitol dehydrogenase
EC=1.1.1.255

Alternative name(s):
NAD-dependent mannitol dehydrogenase

Gene names

Name:

CAD1

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidizes mannitol to mannose. Provides the initial step by which translocated mannitol is committed to central metabolism and, by regulating mannitol pool size, is important in regulating salt tolerance at the cellular level By similarity.
Catalytic activity	D-mannitol + NAD+ = D-mannose + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular_function	mannitol dehydrogenase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Probable mannitol dehydrogenase		PRO_0000160812
Sites
	Metal binding	50	1	Zinc 1; catalytic By similarity
	Metal binding	72	1	Zinc 1; catalytic By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	106	1	Zinc 2 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	117	1	Zinc 2 By similarity
	Metal binding	165	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82515 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D84E383EE0F195A1	FASTA	359	39,027
	10 20 30 40 50 60 MAKSPETELP LKAFGWAARD TSGTLSPFHF SRRENGDDDV SVKILYCGVC HSDLHTLKND 70 80 90 100 110 120 WGFTTYPVVP GHEIVGVVTK VGINVKKFRV GDNVGVGVIV ESCQTCENCN QDLEQYCPKP 130 140 150 160 170 180 VFTYNSPYKG TRTYGGYSDF VVVHQRYVVQ FPDNLPLDAG APLLCAGITV YSPMKYYGMT 190 200 210 220 230 240 EPGKHLGVAG LGGLGHVAIK FGKAFGLKVT VISTSPNKET EAIDKLGADS FLVSKDPEKM 250 260 270 280 290 300 KAAMGTMDYI IDTISAAHSL MPLLGLLKLN GKLVTVGLPS KPLELSVFPL VAGRKLIGGS 310 320 330 340 350 NIGGMKETQE MLDFCGKHNI TADIELIKMH EINTAMERLH KADVKYRFVI DVANSFSSL

References

[1]

"Molecular characterization and expression of a wound-inducible cDNA encoding a novel cinnamyl-alcohol dehydrogenase enzyme in lucerne (Medicago sativa L.)." Brill E.M., Abrahams S., Hayes C.M., Jenkins C.L., Watson J.M. Plant Mol. Biol. 41:279-291(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Siriver. Tissue: Stem.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF083333 mRNA. Translation: AAC35846.1.
3D structure databases
ProteinModelPortal	O82515.
SMR	O82515. Positions 4-358.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.180.10. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTDH_MEDSA

Accession

Primary (citable) accession number: O82515

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program