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Protein

Adenylate kinase 4

Gene

ADK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.By similarity

Catalytic activityi

ATP + AMP = 2 ADP.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641AMPBy similarity
Binding sitei69 – 691AMPBy similarity
Binding sitei125 – 1251AMPBy similarity
Binding sitei160 – 1601ATPBy similarity
Binding sitei193 – 1931AMPBy similarity
Binding sitei204 – 2041AMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 486ATPBy similarity
Nucleotide bindingi90 – 923AMPBy similarity
Nucleotide bindingi118 – 1214AMPBy similarity

GO - Molecular functioni

  • adenylate kinase activity Source: UniProtKB-EC
  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR

GO - Biological processi

  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G63400-MONOMER.
ARA:GQT-2846-MONOMER.
BRENDAi2.7.4.3. 399.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 4 (EC:2.7.4.3)
Short name:
AK 4
Alternative name(s):
ATP-AMP transphosphorylase 4
ATP:AMP phosphotransferase
Adenylate monophosphate kinase 4
Short name:
AMK4
Gene namesi
Name:ADK1
Ordered Locus Names:At5g63400
ORF Names:MLE2.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G63400.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • mitochondrion Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 246245Adenylate kinase 4PRO_0000158940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO82514.
PRIDEiO82514.

PTM databases

iPTMnetiO82514.

Expressioni

Gene expression databases

ExpressionAtlasiO82514. baseline and differential.
GenevisibleiO82514. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G63400.1.

Structurei

3D structure databases

ProteinModelPortaliO82514.
SMRiO82514. Positions 35-240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 9230NMPbindBy similarityAdd
BLAST
Regioni159 – 19638LIDBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
HOGENOMiHOG000238772.
OMAiTEEYNIP.
PhylomeDBiO82514.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O82514-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGGAAADL EDVQTVDLMS ELLRRLKCSQ KPDKRLIFIG PPGSGKGTQS
60 70 80 90 100
PVVKDEYCLC HLSTGDMLRA AVASKTPLGV KAKEAMEKGE LVSDDLVVGI
110 120 130 140 150
IDEAMNKPKC QKGFILDGFP RTVTQAEKLD EMLKRRGTEI DKVLNFAIDD
160 170 180 190 200
AILEERITGR WIHPSSGRSY HTKFAPPKTP GVDDITGEPL IQRKDDNADV
210 220 230 240
LKSRLAAFHS QTQPVIDYYA KKAVLTNIQA EKAPQEVTSE VKKALS
Length:246
Mass (Da):26,932
Last modified:November 2, 2001 - v2
Checksum:i659903FBD84B39C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1529LNFAIDDAI → STLLLMTQS in AAC78478 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082882 mRNA. Translation: AAC78478.1.
AB007649 Genomic DNA. Translation: BAB08805.1.
CP002688 Genomic DNA. Translation: AED97743.1.
AY039945 mRNA. Translation: AAK64049.1.
AY079340 mRNA. Translation: AAL85071.1.
AY085188 mRNA. Translation: AAM61739.1.
RefSeqiNP_201145.1. NM_125735.3. [O82514-1]
UniGeneiAt.23458.

Genome annotation databases

EnsemblPlantsiAT5G63400.1; AT5G63400.1; AT5G63400. [O82514-1]
GeneIDi836459.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082882 mRNA. Translation: AAC78478.1.
AB007649 Genomic DNA. Translation: BAB08805.1.
CP002688 Genomic DNA. Translation: AED97743.1.
AY039945 mRNA. Translation: AAK64049.1.
AY079340 mRNA. Translation: AAL85071.1.
AY085188 mRNA. Translation: AAM61739.1.
RefSeqiNP_201145.1. NM_125735.3. [O82514-1]
UniGeneiAt.23458.

3D structure databases

ProteinModelPortaliO82514.
SMRiO82514. Positions 35-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G63400.1.

PTM databases

iPTMnetiO82514.

Proteomic databases

PaxDbiO82514.
PRIDEiO82514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G63400.1; AT5G63400.1; AT5G63400. [O82514-1]
GeneIDi836459.

Organism-specific databases

TAIRiAT5G63400.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
HOGENOMiHOG000238772.
OMAiTEEYNIP.
PhylomeDBiO82514.

Enzyme and pathway databases

BioCyciARA:AT5G63400-MONOMER.
ARA:GQT-2846-MONOMER.
BRENDAi2.7.4.3. 399.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiO82514.

Gene expression databases

ExpressionAtlasiO82514. baseline and differential.
GenevisibleiO82514. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the cDNA and gene for the Arabidopsis thaliana adenylate kinase."
    Weers B., Thornburg R.
    Plant Gene Register PGR98-166
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKAD4_ARATH
AccessioniPrimary (citable) accession number: O82514
Secondary accession number(s): Q9FMW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 2, 2001
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.