Reviewed, UniProtKB/Swiss-Prot O82514 (KAD1_ARATH)

Names and origin

Protein names

Recommended name:
    Adenylate kinase 1
      Short name=AK 1
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 1

Gene names

Name:	ADK1
Ordered Locus Names:	At5g63400
ORF Names:	MLE2.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This small ubiquitous enzyme is essential for maintenance and cell growth.
Catalytic activity	ATP + AMP = 2 ADP.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrion Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O82514-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Adenylate kinase 1		PRO_0000158940
Regions
	Nucleotide binding	40 – 48	9	ATP By similarity
Experimental info
	Sequence conflict	144 – 152	9	LNFAIDDAI → STLLLMTQS in AAC78478. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 659903FBD84B39C7	FASTA	246	26,932
	10 20 30 40 50 60 MATGGAAADL EDVQTVDLMS ELLRRLKCSQ KPDKRLIFIG PPGSGKGTQS PVVKDEYCLC 70 80 90 100 110 120 HLSTGDMLRA AVASKTPLGV KAKEAMEKGE LVSDDLVVGI IDEAMNKPKC QKGFILDGFP 130 140 150 160 170 180 RTVTQAEKLD EMLKRRGTEI DKVLNFAIDD AILEERITGR WIHPSSGRSY HTKFAPPKTP 190 200 210 220 230 240 GVDDITGEPL IQRKDDNADV LKSRLAAFHS QTQPVIDYYA KKAVLTNIQA EKAPQEVTSE VKKALS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cDNA and gene for the Arabidopsis thaliana adenylate kinase." Weers B., Thornburg R. Plant Gene Register PGR98-166 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082882 mRNA. Translation: AAC78478.1. AB007649 Genomic DNA. Translation: BAB08805.1. AY039945 mRNA. Translation: AAK64049.1. AY079340 mRNA. Translation: AAL85071.1. AY085188 mRNA. Translation: AAM61739.1.
IPI	IPI00531316.
RefSeq	NP_201145.1.
UniGene	At.23458 Rra.685 Rsa.1099
3D structure databases
SMR	O82514. Positions 35-245.
ModBase	Search...
Protein-protein interaction databases
STRING	O82514.
Proteomic databases
PRIDE	O82514.
Genome annotation databases
GeneID	836459.
GenomeReviews	Gene locus AT5G63400 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.28387.
Organism-specific databases
GeneFarm	4387. 444.
TAIR	At5g63400.
Phylogenomic databases
eggNOG	KOG3078.
HOGENOM	HBG630208.
InParanoid	O82514.
OMA	IVIQLQL.
PhylomeDB	O82514.
Enzyme and pathway databases
BRENDA	2.7.4.3. 302.
Gene expression databases
ArrayExpress	O82514.
Genevestigator	O82514.
Family and domain databases
InterPro	IPR006259. Adenyl_kin_sub. IPR000850. Adenylate_kin. IPR007862. Adenylate_kinase_lid-dom. [Graphical view]
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. PF05191. ADK_lid. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
TIGRFAMs	TIGR01351. adk. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAD1_ARATH

Accession

Primary (citable) accession number: O82514
Secondary accession number(s): Q9FMW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 2, 2001
Last modified:	February 9, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)