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Protein

RNA-dependent RNA polymerase 2

Gene

RDR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-dependent direct polymerase involved in the production of small interfering RNAs (siRNAs). Required for the biogenesis of endogenous siRNAs of 24 nucleotide which derive from heterochromatin and DNA repeats such as transposons or endogenous gene tandem repeats, such as repeats present in FWA gene. Involved in transcriptional gene silencing (TGS). Component of the RNA-directed DNA methylation (RdDM) silencing pathway that utilizes siRNAs to guide DNA methyltransferases to asymmetric cytosines. Involved in control of flowering time through RdDM of FWA locus. Required for reception of long-distance mRNA silencing in the shoot. Required for the formation of telomeric siRNAs and the RNA-dependent DNA methylation of asymmetric cytosines in telomeric (5'-CCCTAAA-3') repeats.9 Publications

Miscellaneous

RDR2 is non-functional in the absence of associated Pol IV.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • defense response to fungus Source: TAIR
  • gene silencing Source: TAIR
  • long-distance posttranscriptional gene silencing Source: TAIR
  • production of siRNA involved in chromatin silencing by small RNA Source: CACAO
  • production of siRNA involved in RNA interference Source: TAIR

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, RNA-directed RNA polymerase, Transferase
Biological processRNA-mediated gene silencing

Enzyme and pathway databases

BRENDAi2.7.7.48. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-dependent RNA polymerase 2 (EC:2.7.7.48)
Short name:
AtRDRP2
Alternative name(s):
Protein SILENCING MOVEMENT DEFICIENT 1
RNA-directed RNA polymerase 2
Gene namesi
Name:RDR2
Synonyms:RDRP2, SMD1
Ordered Locus Names:At4g11130
ORF Names:F2P3.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G11130.
TAIRilocus:2136068. AT4G11130.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: TAIR
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: TAIR

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Late flowering and absence of siRNAs derived from FWA tandem repeat regions. Loss of de novo methylation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004046731 – 1133RNA-dependent RNA polymerase 2Add BLAST1133

Proteomic databases

PaxDbiO82504.

PTM databases

iPTMnetiO82504.

Expressioni

Gene expression databases

GenevisibleiO82504. AT.

Interactioni

Subunit structurei

Interacts with NRPD1 and SHH1. Associates with Pol IV complex.3 Publications

Protein-protein interaction databases

BioGridi12013. 4 interactors.
STRINGi3702.AT4G11130.1.

Structurei

3D structure databases

ProteinModelPortaliO82504.
SMRiO82504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RdRP family.Curated

Phylogenomic databases

eggNOGiKOG0988. Eukaryota.
ENOG410XQMQ. LUCA.
HOGENOMiHOG000239464.
InParanoidiO82504.
KOiK11699.
OMAiCYVNREI.
OrthoDBiEOG093600NW.
PhylomeDBiO82504.

Family and domain databases

InterProiView protein in InterPro
IPR007855. RNA-dep_RNA_pol_euk-typ.
PANTHERiPTHR23079. PTHR23079. 1 hit.
PfamiView protein in Pfam
PF05183. RdRP. 1 hit.

Sequencei

Sequence statusi: Complete.

O82504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSETTTNRS TVKISNVPQT IVADELLRFL ELHLGEDTVF ALEIPTTRDN
60 70 80 90 100
WKPRDFARVQ FTTLEVKSRA QLLSSQSKLL FKTHNLRLSE AYDDIIPRPV
110 120 130 140 150
DPRKRLDDIV LTVGFPESDE KRFCALEKWD GVRCWILTEK RRVEFWVWES
160 170 180 190 200
GDCYKIEVRF EDIIETLSCC VNGDASEIDA FLLKLKYGPK VFKRVTVHIA
210 220 230 240 250
TKFKSDRYRF CKEDFDFMWI RTTDFSGSKS IGTSTCFCLE VHNGSTMLDI
260 270 280 290 300
FSGLPYYRED TLSLTYVDGK TFASAAQIVP LLNAAILGLE FPYEILFQLN
310 320 330 340 350
ALVHAQKISL FAASDMELIK ILRGMSLETA LVILKKLHQQ SSICYDPVFF
360 370 380 390 400
VKTQMQSVVK KMKHSPASAY KRLTEQNIMS CQRAYVTPSK IYLLGPELET
410 420 430 440 450
ANYVVKNFAE HVSDFMRVTF VEEDWSKLPA NALSVNSKEG YFVKPSRTNI
460 470 480 490 500
YNRVLSILGE GITVGPKRFE FLAFSASQLR GNSVWMFASN EKVKAEDIRE
510 520 530 540 550
WMGCFRKIRS ISKCAARMGQ LFSASRQTLI VRAQDVEQIP DIEVTTDGAD
560 570 580 590 600
YCFSDGIGKI SLAFAKQVAQ KCGLSHVPSA FQIRYGGYKG VIAVDRSSFR
610 620 630 640 650
KLSLRDSMLK FDSNNRMLNV TRWTESMPCF LNREIICLLS TLGIEDAMFE
660 670 680 690 700
AMQAVHLSML GNMLEDRDAA LNVLQKLSGE NSKNLLVKML LQGYAPSSEP
710 720 730 740 750
YLSMMLRVHH ESQLSELKSR CRILVPKGRI LIGCMDEMGI LEYGQVYVRV
760 770 780 790 800
TLTKAELKSR DQSYFRKIDE ETSVVIGKVV VTKNPCLHPG DIRVLDAIYE
810 820 830 840 850
VHFEEKGYLD CIIFPQKGER PHPNECSGGD LDGDQFFVSW DEKIIPSEMD
860 870 880 890 900
PPMDYAGSRP RLMDHDVTLE EIHKFFVDYM ISDTLGVIST AHLVHADRDP
910 920 930 940 950
EKARSQKCLE LANLHSRAVD FAKTGAPAEM PYALKPREFP DFLERFEKPT
960 970 980 990 1000
YISESVFGKL YRAVKSSLAQ RKPEAESEDT VAYDVTLEEA GFESFIETAK
1010 1020 1030 1040 1050
AHRDMYGEKL TSLMIYYGAA NEEEILTGIL KTKEMYLARD NRRYGDMKDR
1060 1070 1080 1090 1100
ITLSVKDLHK EAMGWFEKSC EDEQQKKKLA SAWYYVTYNP NHRDEKLTFL
1110 1120 1130
SFPWIVGDVL LDIKAENAQR QSVEEKTSGL VSI
Length:1,133
Mass (Da):129,325
Last modified:November 1, 1998 - v1
Checksum:i32B72C4E429B20B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080120 Genomic DNA. Translation: AAC35535.1.
AL049876 Genomic DNA. Translation: CAB43048.1.
AL161531 Genomic DNA. Translation: CAB81214.1.
CP002687 Genomic DNA. Translation: AEE82976.1.
PIRiT01920.
RefSeqiNP_192851.1. NM_117183.3.
UniGeneiAt.43417.

Genome annotation databases

EnsemblPlantsiAT4G11130.1; AT4G11130.1; AT4G11130.
GeneIDi826714.
GrameneiAT4G11130.1; AT4G11130.1; AT4G11130.
KEGGiath:AT4G11130.

Similar proteinsi

Entry informationi

Entry nameiRDR2_ARATH
AccessioniPrimary (citable) accession number: O82504
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: November 1, 1998
Last modified: April 12, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families