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Protein

N6-adenosine-methyltransferase MT-A70-like

Gene

MTA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

GO - Molecular functioni

GO - Biological processi

  • mRNA methylation Source: TAIR
  • RNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT4G10760-MONOMER.
ReactomeiREACT_335264. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
N6-adenosine-methyltransferase MT-A70-like (EC:2.1.1.62)
Alternative name(s):
Protein EMBRYO DEFECTIVE 1706
Gene namesi
Name:MTA
Synonyms:EMB1706
Ordered Locus Names:At4g10760
ORF Names:T12H20.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G10760.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • nuclear speck Source: TAIR
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality: arrest at the globular stage of embryo development. Arrested seeds are deficient in mRNAs containing N6-methyladenosine (m6A).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 685685N6-adenosine-methyltransferase MT-A70-likePRO_0000207632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei664 – 6641Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82486.
PRIDEiO82486.

Interactioni

Subunit structurei

Interacts with FIP37.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FIP37Q9ZSZ85EBI-1797380,EBI-1641243

Protein-protein interaction databases

IntActiO82486. 1 interaction.
STRINGi3702.AT4G10760.1.

Structurei

3D structure databases

ProteinModelPortaliO82486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi259 – 29032Met-richAdd
BLAST

Sequence similaritiesi

Belongs to the MT-A70-like family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4725.
HOGENOMiHOG000077476.
InParanoidiO82486.
KOiK05925.
OMAiRAMVAVC.
PhylomeDBiO82486.

Family and domain databases

InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METESDDATI TVVKDMRVRL ENRIRTQHDA HLDLLSSLQS IVPDIVPSLD
60 70 80 90 100
LSLKLISSFT NRPFVATPPL PEPKVEKKHH PIVKLGTQLQ QLHGHDSKSM
110 120 130 140 150
LVDSNQRDAE ADGSSGSPMA LVRAMVAECL LQRVPFSPTD SSTVLRKLEN
160 170 180 190 200
DQNARPAEKA ALRDLGGECG PILAVETALK SMAEENGSVE LEEFEVSGKP
210 220 230 240 250
RIMVLAIDRT RLLKELPESF QGNNESNRVV ETPNSIENAT VSGGGFGVSG
260 270 280 290 300
SGNFPRPEMW GGDPNMGFRP MMNAPRGMQM MGMHHPMGIM GRPPPFPLPL
310 320 330 340 350
PLPVPSNQKL RSEEEDLKDV EALLSKKSFK EKQQSRTGEE LLDLIHRPTA
360 370 380 390 400
KEAATAAKFK SKGGSQVKYY CRYLTKEDCR LQSGSHIACN KRHFRRLIAS
410 420 430 440 450
HTDVSLGDCS FLDTCRHMKT CKYVHYELDM ADAMMAGPDK ALKPLRADYC
460 470 480 490 500
SEAELGEAQW INCDIRSFRM DILGTFGVVM ADPPWDIHME LPYGTMADDE
510 520 530 540 550
MRTLNVPSLQ TDGLIFLWVT GRAMELGREC LELWGYKRVE EIIWVKTNQL
560 570 580 590 600
QRIIRTGRTG HWLNHSKEHC LVGIKGNPEV NRNIDTDVIV AEVRETSRKP
610 620 630 640 650
DEMYAMLERI MPRARKLELF ARMHNAHAGW LSLGNQLNGV RLINEGLRAR
660 670 680
FKASYPEIDV QPPSPPRASA METDNEPMAI DSITA
Length:685
Mass (Da):76,645
Last modified:August 16, 2005 - v2
Checksum:i1DBB54FBE7C5034D
GO

Sequence cautioni

The sequence AAC35526.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AK227385 differs from that shown. Reason: Frameshift at position 206. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080119 Genomic DNA. Translation: AAC35526.1. Sequence problems.
AL161518 Genomic DNA. Translation: CAB81177.1.
CP002687 Genomic DNA. Translation: AEE82925.1.
AK227385 mRNA. No translation available.
PIRiE85112.
T01901.
RefSeqiNP_192814.1. NM_117144.3.
UniGeneiAt.20163.
At.63691.

Genome annotation databases

EnsemblPlantsiAT4G10760.1; AT4G10760.1; AT4G10760.
GeneIDi826670.
KEGGiath:AT4G10760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080119 Genomic DNA. Translation: AAC35526.1. Sequence problems.
AL161518 Genomic DNA. Translation: CAB81177.1.
CP002687 Genomic DNA. Translation: AEE82925.1.
AK227385 mRNA. No translation available.
PIRiE85112.
T01901.
RefSeqiNP_192814.1. NM_117144.3.
UniGeneiAt.20163.
At.63691.

3D structure databases

ProteinModelPortaliO82486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO82486. 1 interaction.
STRINGi3702.AT4G10760.1.

Proteomic databases

PaxDbiO82486.
PRIDEiO82486.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G10760.1; AT4G10760.1; AT4G10760.
GeneIDi826670.
KEGGiath:AT4G10760.

Organism-specific databases

GeneFarmi4529. 449.
TAIRiAT4G10760.

Phylogenomic databases

eggNOGiCOG4725.
HOGENOMiHOG000077476.
InParanoidiO82486.
KOiK05925.
OMAiRAMVAVC.
PhylomeDBiO82486.

Enzyme and pathway databases

BioCyciARA:AT4G10760-MONOMER.
ReactomeiREACT_335264. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

PROiO82486.

Family and domain databases

InterProiIPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor."
    Zhong S., Li H., Bodi Z., Button J., Vespa L., Herzog M., Fray R.G.
    Plant Cell 20:1278-1288(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FIP37, FUNCTION, DISRUPTION PHENOTYPE.
  5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiMTA70_ARATH
AccessioniPrimary (citable) accession number: O82486
Secondary accession number(s): Q9M0N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: August 16, 2005
Last modified: July 22, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.