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O82486

- MTA70_ARATH

UniProt

O82486 - MTA70_ARATH

Protein

N6-adenosine-methyltransferase MT-A70-like

Gene

MTA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (16 Aug 2005)
      Previous versions | rss
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    Functioni

    Probable N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

    GO - Molecular functioni

    1. mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: UniProtKB
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA methylation Source: TAIR
    2. RNA methylation Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT4G10760-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N6-adenosine-methyltransferase MT-A70-like (EC:2.1.1.62)
    Alternative name(s):
    Protein EMBRYO DEFECTIVE 1706
    Gene namesi
    Name:MTA
    Synonyms:EMB1706
    Ordered Locus Names:At4g10760
    ORF Names:T12H20.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G10760.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. nuclear speck Source: TAIR
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryonic lethality: arrest at the globular stage of embryo development. Arrested seeds are deficient in mRNAs containing N6-methyladenosine (m6A).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 685685N6-adenosine-methyltransferase MT-A70-likePRO_0000207632Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei664 – 6641Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO82486.
    PRIDEiO82486.

    Expressioni

    Gene expression databases

    GenevestigatoriO82486.

    Interactioni

    Subunit structurei

    Interacts with FIP37.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FIP37Q9ZSZ85EBI-1797380,EBI-1641243

    Protein-protein interaction databases

    IntActiO82486. 1 interaction.
    STRINGi3702.AT4G10760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82486.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi259 – 29032Met-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MT-A70-like family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4725.
    HOGENOMiHOG000077476.
    InParanoidiO82486.
    OMAiEDCRRQT.
    PhylomeDBiO82486.

    Family and domain databases

    InterProiIPR007757. MT-A70-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF05063. MT-A70. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51143. MT_A70. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O82486-1 [UniParc]FASTAAdd to Basket

    « Hide

    METESDDATI TVVKDMRVRL ENRIRTQHDA HLDLLSSLQS IVPDIVPSLD    50
    LSLKLISSFT NRPFVATPPL PEPKVEKKHH PIVKLGTQLQ QLHGHDSKSM 100
    LVDSNQRDAE ADGSSGSPMA LVRAMVAECL LQRVPFSPTD SSTVLRKLEN 150
    DQNARPAEKA ALRDLGGECG PILAVETALK SMAEENGSVE LEEFEVSGKP 200
    RIMVLAIDRT RLLKELPESF QGNNESNRVV ETPNSIENAT VSGGGFGVSG 250
    SGNFPRPEMW GGDPNMGFRP MMNAPRGMQM MGMHHPMGIM GRPPPFPLPL 300
    PLPVPSNQKL RSEEEDLKDV EALLSKKSFK EKQQSRTGEE LLDLIHRPTA 350
    KEAATAAKFK SKGGSQVKYY CRYLTKEDCR LQSGSHIACN KRHFRRLIAS 400
    HTDVSLGDCS FLDTCRHMKT CKYVHYELDM ADAMMAGPDK ALKPLRADYC 450
    SEAELGEAQW INCDIRSFRM DILGTFGVVM ADPPWDIHME LPYGTMADDE 500
    MRTLNVPSLQ TDGLIFLWVT GRAMELGREC LELWGYKRVE EIIWVKTNQL 550
    QRIIRTGRTG HWLNHSKEHC LVGIKGNPEV NRNIDTDVIV AEVRETSRKP 600
    DEMYAMLERI MPRARKLELF ARMHNAHAGW LSLGNQLNGV RLINEGLRAR 650
    FKASYPEIDV QPPSPPRASA METDNEPMAI DSITA 685
    Length:685
    Mass (Da):76,645
    Last modified:August 16, 2005 - v2
    Checksum:i1DBB54FBE7C5034D
    GO

    Sequence cautioni

    The sequence AK227385 differs from that shown. Reason: Frameshift at position 206.
    The sequence AAC35526.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF080119 Genomic DNA. Translation: AAC35526.1. Sequence problems.
    AL161518 Genomic DNA. Translation: CAB81177.1.
    CP002687 Genomic DNA. Translation: AEE82925.1.
    AK227385 mRNA. No translation available.
    PIRiE85112.
    T01901.
    RefSeqiNP_192814.1. NM_117144.3.
    UniGeneiAt.20163.
    At.63691.

    Genome annotation databases

    EnsemblPlantsiAT4G10760.1; AT4G10760.1; AT4G10760.
    GeneIDi826670.
    KEGGiath:AT4G10760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF080119 Genomic DNA. Translation: AAC35526.1 . Sequence problems.
    AL161518 Genomic DNA. Translation: CAB81177.1 .
    CP002687 Genomic DNA. Translation: AEE82925.1 .
    AK227385 mRNA. No translation available.
    PIRi E85112.
    T01901.
    RefSeqi NP_192814.1. NM_117144.3.
    UniGenei At.20163.
    At.63691.

    3D structure databases

    ProteinModelPortali O82486.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O82486. 1 interaction.
    STRINGi 3702.AT4G10760.1-P.

    Proteomic databases

    PaxDbi O82486.
    PRIDEi O82486.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G10760.1 ; AT4G10760.1 ; AT4G10760 .
    GeneIDi 826670.
    KEGGi ath:AT4G10760.

    Organism-specific databases

    GeneFarmi 4529. 449.
    TAIRi AT4G10760.

    Phylogenomic databases

    eggNOGi COG4725.
    HOGENOMi HOG000077476.
    InParanoidi O82486.
    OMAi EDCRRQT.
    PhylomeDBi O82486.

    Enzyme and pathway databases

    BioCyci ARA:AT4G10760-MONOMER.

    Miscellaneous databases

    PROi O82486.

    Gene expression databases

    Genevestigatori O82486.

    Family and domain databases

    InterProi IPR007757. MT-A70-like.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF05063. MT-A70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51143. MT_A70. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor."
      Zhong S., Li H., Bodi Z., Button J., Vespa L., Herzog M., Fray R.G.
      Plant Cell 20:1278-1288(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FIP37, FUNCTION, DISRUPTION PHENOTYPE.
    5. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiMTA70_ARATH
    AccessioniPrimary (citable) accession number: O82486
    Secondary accession number(s): Q9M0N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: August 16, 2005
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3