Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase, cytoplasmic

Gene

At5g26710

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93ATPBy similarity1
Binding sitei227ATPBy similarity1
Binding sitei411GlutamateBy similarity1
Binding sitei414ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi448 – 452ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G26710-MONOMER.
BRENDAi6.1.1.17. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, cytoplasmicCurated (EC:6.1.1.17Curated)
Alternative name(s):
GluRSAt1 Publication
Glutamyl-tRNA synthetaseCurated
Short name:
GluRSCurated
Gene namesi
Ordered Locus Names:At5g26710Imported
ORF Names:F21E10.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26710.
TAIRilocus:2146769. AT5G26710.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004335431 – 719Glutamate--tRNA ligase, cytoplasmicAdd BLAST719

Proteomic databases

PaxDbiO82462.

Expressioni

Gene expression databases

GenevisibleiO82462. AT.

Interactioni

Subunit structurei

Interacts with GLN2, COL4 AND RPP13L4/ZAR1.1 Publication

Protein-protein interaction databases

DIPiDIP-48341N.
STRINGi3702.AT5G26710.1.

Structurei

3D structure databases

ProteinModelPortaliO82462.
SMRiO82462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 219Glutamate bindingBy similarity3
Regioni393 – 397Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi220 – 230"HIGH" regionCuratedAdd BLAST11
Motifi448 – 452"KMSKS" regionCurated5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1147. Eukaryota.
COG0008. LUCA.
HOGENOMiHOG000259234.
InParanoidiO82462.
KOiK01885.
OMAiDTKMYKV.
OrthoDBiEOG093602VY.
PhylomeDBiO82462.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_02076. Glu_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF14497. GST_C_3. 1 hit.
PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

O82462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGMKLSFPP ESPPLSVIVA LSLSASPVTI DSSAAATTVP SFVFSDGRKL
60 70 80 90 100
NGATVLLRYV GRSAKKLPDF YGNNAFDSSQ IDEWVDYASV FSSGSEFENA
110 120 130 140 150
CGRVDKYLES STFLVGHSLS IADVAIWSAL AGTGQRWESL RKSKKYQSLV
160 170 180 190 200
RWFNSILDEY SEVLNKVLAT YVKKGSGKPV AAPKSKDSQQ AVKGDGQDKG
210 220 230 240 250
KPEVDLPEAE IGKVKLRFAP EPSGYLHIGH AKAALLNKYF AERYQGEVIV
260 270 280 290 300
RFDDTNPAKE SNEFVDNLVK DIGTLGIKYE KVTYTSDYFP ELMDMAEKLM
310 320 330 340 350
REGKAYVDDT PREQMQKERM DGIDSKCRNH SVEENLKLWK EMIAGSERGL
360 370 380 390 400
QCCVRGKFNM QDPNKAMRDP VYYRCNPMSH HRIGDKYKIY PTYDFACPFV
410 420 430 440 450
DSLEGITHAL RSSEYHDRNA QYFKVLEDMG LRQVQLYEFS RLNLVFTLLS
460 470 480 490 500
KRKLLWFVQT GLVDGWDDPR FPTVQGIVRR GLKIEALIQF ILEQGASKNL
510 520 530 540 550
NLMEWDKLWS INKRIIDPVC PRHTAVVAER RVLFTLTDGP DEPFVRMIPK
560 570 580 590 600
HKKFEGAGEK ATTFTKSIWL EEADASAISV GEEVTLMDWG NAIVKEITKD
610 620 630 640 650
EEGRVTALSG VLNLQGSVKT TKLKLTWLPD TNELVNLTLT EFDYLITKKK
660 670 680 690 700
LEDDDEVADF VNPNTKKETL ALGDSNMRNL KCGDVIQLER KGYFRCDVPF
710
VKSSKPIVLF SIPDGRAAK
Length:719
Mass (Da):81,065
Last modified:November 1, 1998 - v1
Checksum:i0778C243219DA24C
GO

Sequence cautioni

The sequence AAC13597 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067773 mRNA. Translation: AAC36469.1.
AF058914 Genomic DNA. Translation: AAC13597.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93573.1.
AY099592 mRNA. Translation: AAM20443.1.
BT000248 mRNA. Translation: AAN15567.1.
AK226448 mRNA. Translation: BAE98590.1.
PIRiT01200.
T52043.
RefSeqiNP_850874.1. NM_180543.3.
UniGeneiAt.49067.
At.71213.

Genome annotation databases

EnsemblPlantsiAT5G26710.1; AT5G26710.1; AT5G26710.
GeneIDi832718.
GrameneiAT5G26710.1; AT5G26710.1; AT5G26710.
KEGGiath:AT5G26710.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYEC_ARATH
AccessioniPrimary (citable) accession number: O82462
Secondary accession number(s): O65253
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: November 1, 1998
Last modified: June 7, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families