Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase, cytoplasmic

Gene

At5g26710

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93ATPBy similarity1
Binding sitei227ATPBy similarity1
Binding sitei411GlutamateBy similarity1
Binding sitei414ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi448 – 452ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.17 399

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, cytoplasmicCurated (EC:6.1.1.17Curated)
Alternative name(s):
GluRSAt1 Publication
Glutamyl-tRNA synthetaseCurated
Short name:
GluRSCurated
Gene namesi
Ordered Locus Names:At5g26710Imported
ORF Names:F21E10.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26710
TAIRilocus:2146769 AT5G26710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004335431 – 719Glutamate--tRNA ligase, cytoplasmicAdd BLAST719

Proteomic databases

PaxDbiO82462

Expressioni

Gene expression databases

ExpressionAtlasiO82462 baseline and differential
GenevisibleiO82462 AT

Interactioni

Subunit structurei

Interacts with GLN2, COL4 AND RPP13L4/ZAR1.1 Publication

Protein-protein interaction databases

DIPiDIP-48341N
IntActiO82462, 3 interactors
STRINGi3702.AT5G26710.1

Structurei

3D structure databases

ProteinModelPortaliO82462
SMRiO82462
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 219Glutamate bindingBy similarity3
Regioni393 – 397Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi220 – 230"HIGH" regionCuratedAdd BLAST11
Motifi448 – 452"KMSKS" regionCurated5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1147 Eukaryota
COG0008 LUCA
HOGENOMiHOG000259234
InParanoidiO82462
KOiK01885
OMAiFARMNFI
OrthoDBiEOG093602VY
PhylomeDBiO82462

Family and domain databases

Gene3Di2.40.240.10, 3 hits
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282 Glutathione-S-Trfase_C_sf
IPR004046 GST_C
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

O82462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGMKLSFPP ESPPLSVIVA LSLSASPVTI DSSAAATTVP SFVFSDGRKL
60 70 80 90 100
NGATVLLRYV GRSAKKLPDF YGNNAFDSSQ IDEWVDYASV FSSGSEFENA
110 120 130 140 150
CGRVDKYLES STFLVGHSLS IADVAIWSAL AGTGQRWESL RKSKKYQSLV
160 170 180 190 200
RWFNSILDEY SEVLNKVLAT YVKKGSGKPV AAPKSKDSQQ AVKGDGQDKG
210 220 230 240 250
KPEVDLPEAE IGKVKLRFAP EPSGYLHIGH AKAALLNKYF AERYQGEVIV
260 270 280 290 300
RFDDTNPAKE SNEFVDNLVK DIGTLGIKYE KVTYTSDYFP ELMDMAEKLM
310 320 330 340 350
REGKAYVDDT PREQMQKERM DGIDSKCRNH SVEENLKLWK EMIAGSERGL
360 370 380 390 400
QCCVRGKFNM QDPNKAMRDP VYYRCNPMSH HRIGDKYKIY PTYDFACPFV
410 420 430 440 450
DSLEGITHAL RSSEYHDRNA QYFKVLEDMG LRQVQLYEFS RLNLVFTLLS
460 470 480 490 500
KRKLLWFVQT GLVDGWDDPR FPTVQGIVRR GLKIEALIQF ILEQGASKNL
510 520 530 540 550
NLMEWDKLWS INKRIIDPVC PRHTAVVAER RVLFTLTDGP DEPFVRMIPK
560 570 580 590 600
HKKFEGAGEK ATTFTKSIWL EEADASAISV GEEVTLMDWG NAIVKEITKD
610 620 630 640 650
EEGRVTALSG VLNLQGSVKT TKLKLTWLPD TNELVNLTLT EFDYLITKKK
660 670 680 690 700
LEDDDEVADF VNPNTKKETL ALGDSNMRNL KCGDVIQLER KGYFRCDVPF
710
VKSSKPIVLF SIPDGRAAK
Length:719
Mass (Da):81,065
Last modified:November 1, 1998 - v1
Checksum:i0778C243219DA24C
GO

Sequence cautioni

The sequence AAC13597 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067773 mRNA Translation: AAC36469.1
AF058914 Genomic DNA Translation: AAC13597.1 Sequence problems.
CP002688 Genomic DNA Translation: AED93573.1
AY099592 mRNA Translation: AAM20443.1
BT000248 mRNA Translation: AAN15567.1
AK226448 mRNA Translation: BAE98590.1
PIRiT01200
T52043
RefSeqiNP_850874.1, NM_180543.3
UniGeneiAt.49067
At.71213

Genome annotation databases

EnsemblPlantsiAT5G26710.1; AT5G26710.1; AT5G26710
GeneIDi832718
GrameneiAT5G26710.1; AT5G26710.1; AT5G26710
KEGGiath:AT5G26710

Similar proteinsi

Entry informationi

Entry nameiSYEC_ARATH
AccessioniPrimary (citable) accession number: O82462
Secondary accession number(s): O65253
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health