ID   GSTF2_ORYSJ             Reviewed;         215 AA.
AC   O82451; O65858; Q7F5M8;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   27-MAR-2024, entry version 132.
DE   RecName: Full=Probable glutathione S-transferase GSTF2;
DE            EC=2.5.1.18;
DE   AltName: Full=GST-II;
GN   Name=GSTF2; Synonyms=RGST II;
GN   OrderedLocusNames=Os01g0764000, LOC_Os01g55830; ORFNames=P0403C05.5;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Lemont; TISSUE=Root;
RA   Wu J., Cramer C., Hatzios K.K.;
RT   "Isolation of a full-length cDNA encoding the second glutathione S-
RT   transferase from rice (Oryza sativa).";
RL   (er) Plant Gene Register PGR98-136(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   PROTEIN SEQUENCE OF 2-11, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare;
RC   TISSUE=Anther, Callus, Panicle, Root, Sheath, and Stem;
RX   PubMed=14681440; DOI=10.1093/nar/gkh020;
RA   Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.;
RT   "Rice proteome database based on two-dimensional polyacrylamide gel
RT   electrophoresis: its status in 2003.";
RL   Nucleic Acids Res. 32:D388-D392(2004).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 50-195.
RC   STRAIN=cv. Lemont;
RA   Wu J., Cramer C., Hatzios K.K.;
RT   "Nucleotide sequence of a cDNA encoding the second glutathione S-
RT   transferase from rice (Oryza sativa).";
RL   (er) Plant Gene Register PGR98-119(1998).
RN   [8]
RP   CHARACTERIZATION, TISSUE SPECIFICITY, AND INDUCTION.
RC   STRAIN=cv. Lemont;
RX   DOI=10.1034/j.1399-3054.1999.105116.x;
RA   Wu J., Cramer C., Hatzios K.K.;
RT   "Characterization of two cDNAs encoding glutathione S-transferases in rice
RT   and induction of their transcripts by the herbicide safener fenclorim.";
RL   Physiol. Plantarum 105:102-108(1999).
RN   [9]
RP   NOMENCLATURE, AND INDUCTION.
RX   PubMed=15069639; DOI=10.1007/s00438-004-1006-8;
RA   Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.;
RT   "Organisation and structural evolution of the rice glutathione S-
RT   transferase gene family.";
RL   Mol. Genet. Genomics 271:511-521(2004).
CC   -!- FUNCTION: Conjugation of reduced glutathione to a wide number of
CC       exogenous and endogenous hydrophobic electrophiles.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC   -!- TISSUE SPECIFICITY: Constitutively expressed in roots. Expressed in
CC       anthers, callus, panicles, sheaths and stems (at protein level).
CC       {ECO:0000269|PubMed:14681440, ECO:0000269|Ref.8}.
CC   -!- INDUCTION: By the herbicide safener fenclorim. By drought stress.
CC       {ECO:0000269|PubMed:15069639, ECO:0000269|Ref.8}.
CC   -!- SIMILARITY: Belongs to the GST superfamily. Phi family. {ECO:0000305}.
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DR   EMBL; AF062403; AAC64007.1; -; mRNA.
DR   EMBL; AP003239; BAB63585.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS74483.1; -; Genomic_DNA.
DR   EMBL; AK058894; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AK059818; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AK099142; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AJ002381; CAA05355.1; -; mRNA.
DR   PIR; T03989; T03989.
DR   RefSeq; XP_015614423.1; XM_015758937.1.
DR   AlphaFoldDB; O82451; -.
DR   SMR; O82451; -.
DR   BioGRID; 795924; 1.
DR   STRING; 39947.O82451; -.
DR   PaxDb; 39947-O82451; -.
DR   EnsemblPlants; Os01t0764000-01; Os01t0764000-01; Os01g0764000.
DR   EnsemblPlants; Os01t0764000-03; Os01t0764000-03; Os01g0764000.
DR   GeneID; 4326887; -.
DR   Gramene; Os01t0764000-01; Os01t0764000-01; Os01g0764000.
DR   Gramene; Os01t0764000-03; Os01t0764000-03; Os01g0764000.
DR   KEGG; osa:4326887; -.
DR   eggNOG; KOG0867; Eukaryota.
DR   HOGENOM; CLU_011226_5_1_1; -.
DR   InParanoid; O82451; -.
DR   OMA; PRIIEWV; -.
DR   OrthoDB; 444791at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   ExpressionAtlas; O82451; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0043295; F:glutathione binding; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   CDD; cd03187; GST_C_Phi; 1.
DR   CDD; cd03053; GST_N_Phi; 1.
DR   Gene3D; 1.20.1050.10; -; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR004046; GST_C.
DR   InterPro; IPR034347; GST_Phi_C.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR43900:SF39; GLUTATHIONE S-TRANSFERASE GSTF2-RELATED; 1.
DR   PANTHER; PTHR43900; GLUTATHIONE S-TRANSFERASE RHO; 1.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SFLD; SFLDG01154; Main.5:_Phi-like; 1.
DR   SUPFAM; SSF47616; GST C-terminal domain-like; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
DR   Genevisible; O82451; OS.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:14681440"
FT   CHAIN           2..215
FT                   /note="Probable glutathione S-transferase GSTF2"
FT                   /id="PRO_0000185845"
FT   DOMAIN          2..83
FT                   /note="GST N-terminal"
FT   DOMAIN          88..215
FT                   /note="GST C-terminal"
FT   BINDING         12
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         41..42
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         54..55
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   BINDING         67..68
FT                   /ligand="glutathione"
FT                   /ligand_id="ChEBI:CHEBI:57925"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        190
FT                   /note="H -> Q (in Ref. 7; CAA05355)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   215 AA;  23977 MW;  F956905A623D07D0 CRC64;
     MAPMKLYGST LSWNVTRCVA VLEEAGAEYE IVPLDFSKGE HKAPDHLARN PFGQVPALQD
     GDLFLWESRA ICKYVCRKNK PELLKDGDLK ESAMVDVWLE VESNQYTPAL NPILFQCLIR
     PMMFGAPPDE KVVEENLEKL KKVLEVYEAR LTKCKYLAGD YISVADLSHV AGTVCLGATP
     HASVLDAYPH VKAWWTDLMA RPSSQKVASL MKPPA
//