O82451 (GSTF2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione S-transferase GSTF2
EC=2.5.1.18

Alternative name(s):
GST-II

Gene names

Name:	GSTF2
Synonyms:	RGST II
Ordered Locus Names:	Os01g0764000, LOC_Os01g55830
ORF Names:	P0403C05.5

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Tissue specificity	Constitutively expressed in roots. Expressed in anthers, callus, panicles, sheaths and stems (at protein level). Ref.4 Ref.6
Induction	By the herbicide safener fenclorim. By drought stress. Ref.6 Ref.7
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 215	214	Probable glutathione S-transferase GSTF2		PRO_0000185845
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	88 – 215	128	GST C-terminal
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
Experimental info
	Sequence conflict	190	1	H → Q in CAA05355. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O82451 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F956905A623D07D0	FASTA	215	23,977
	10 20 30 40 50 60 MAPMKLYGST LSWNVTRCVA VLEEAGAEYE IVPLDFSKGE HKAPDHLARN PFGQVPALQD 70 80 90 100 110 120 GDLFLWESRA ICKYVCRKNK PELLKDGDLK ESAMVDVWLE VESNQYTPAL NPILFQCLIR 130 140 150 160 170 180 PMMFGAPPDE KVVEENLEKL KKVLEVYEAR LTKCKYLAGD YISVADLSHV AGTVCLGATP 190 200 210 HASVLDAYPH VKAWWTDLMA RPSSQKVASL MKPPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a full-length cDNA encoding the second glutathione S-transferase from rice (Oryza sativa)." Wu J., Cramer C., Hatzios K.K. Plant Gene Register PGR98-136 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lemont. Tissue: Root.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, TISSUE SPECIFICITY. Strain: cv. Nipponbare. Tissue: Anther, Callus, Panicle, Root, Sheath and Stem.
[5]	"Nucleotide sequence of a cDNA encoding the second glutathione S-transferase from rice (Oryza sativa)." Wu J., Cramer C., Hatzios K.K. Plant Gene Register PGR98-119 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-195. Strain: cv. Lemont.
[6]	"Characterization of two cDNAs encoding glutathione S-transferases in rice and induction of their transcripts by the herbicide safener fenclorim." Wu J., Cramer C., Hatzios K.K. Physiol. Plantarum 105:102-108(1999) Cited for: CHARACTERIZATION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Lemont.
[7]	"Organisation and structural evolution of the rice glutathione S-transferase gene family." Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C. Mol. Genet. Genomics 271:511-521(2004) [PubMed: 15069639] [Abstract] Cited for: NOMENCLATURE, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF062403 mRNA. Translation: AAC64007.1. AP003239 Genomic DNA. Translation: BAB63585.1. AK058894 mRNA. No translation available. AK059818 mRNA. No translation available. AK099142 mRNA. No translation available. AJ002381 mRNA. Translation: CAA05355.1.
PIR	T03989.
RefSeq	NP_001044339.1. NM_001050874.1.
UniGene	Os.2241.
3D structure databases
ProteinModelPortal	O82451.
SMR	O82451. Positions 2-215.
ModBase	Search...
Protein-protein interaction databases
STRING	O82451.
2D gel databases
ANU-2DPAGE	O82451.
Proteomic databases
PRIDE	O82451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g55830.1; LOC_Os01g55830.1; LOC_Os01g55830.
GeneID	4326887.
KEGG	osa:4326887.
Organism-specific databases
Gramene	O82451.
Phylogenomic databases
OMA	LEVESNQ.
PhylomeDB	O82451.
ProtClustDB	CLSN2691874.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF2_ORYSJ

Accession

Primary (citable) accession number: O82451
Secondary accession number(s): O65858, Q7F5M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program