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O82451 (GSTF2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutathione S-transferase GSTF2

EC=2.5.1.18
Alternative name(s):
GST-II
Gene names
Name:GSTF2
Synonyms:RGST II
Ordered Locus Names:Os01g0764000, LOC_Os01g55830
ORF Names:P0403C05.5
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Tissue specificity

Constitutively expressed in roots. Expressed in anthers, callus, panicles, sheaths and stems (at protein level). Ref.4 Ref.6

Induction

By the herbicide safener fenclorim. By drought stress. Ref.6 Ref.7

Sequence similarities

Belongs to the GST superfamily. Phi family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Molecular functionTransferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 215214Probable glutathione S-transferase GSTF2
PRO_0000185845

Regions

Domain2 – 8382GST N-terminal
Domain88 – 215128GST C-terminal
Region41 – 422Glutathione binding By similarity
Region54 – 552Glutathione binding By similarity
Region67 – 682Glutathione binding By similarity

Sites

Binding site121Glutathione By similarity

Experimental info

Sequence conflict1901H → Q in CAA05355. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O82451 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F956905A623D07D0

FASTA21523,977
        10         20         30         40         50         60 
MAPMKLYGST LSWNVTRCVA VLEEAGAEYE IVPLDFSKGE HKAPDHLARN PFGQVPALQD 

        70         80         90        100        110        120 
GDLFLWESRA ICKYVCRKNK PELLKDGDLK ESAMVDVWLE VESNQYTPAL NPILFQCLIR 

       130        140        150        160        170        180 
PMMFGAPPDE KVVEENLEKL KKVLEVYEAR LTKCKYLAGD YISVADLSHV AGTVCLGATP 

       190        200        210 
HASVLDAYPH VKAWWTDLMA RPSSQKVASL MKPPA 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a full-length cDNA encoding the second glutathione S-transferase from rice (Oryza sativa)."
Wu J., Cramer C., Hatzios K.K.
Plant Gene Register PGR98-136
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Lemont.
Tissue: Root.
[2]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[4]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, TISSUE SPECIFICITY.
Strain: cv. Nipponbare.
Tissue: Anther, Callus, Panicle, Root, Sheath and Stem.
[5]"Nucleotide sequence of a cDNA encoding the second glutathione S-transferase from rice (Oryza sativa)."
Wu J., Cramer C., Hatzios K.K.
Plant Gene Register PGR98-119
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 50-195.
Strain: cv. Lemont.
[6]"Characterization of two cDNAs encoding glutathione S-transferases in rice and induction of their transcripts by the herbicide safener fenclorim."
Wu J., Cramer C., Hatzios K.K.
Physiol. Plantarum 105:102-108(1999)
Cited for: CHARACTERIZATION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Lemont.
[7]"Organisation and structural evolution of the rice glutathione S-transferase gene family."
Soranzo N., Sari Gorla M., Mizzi L., De Toma G., Frova C.
Mol. Genet. Genomics 271:511-521(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF062403 mRNA. Translation: AAC64007.1.
AP003239 Genomic DNA. Translation: BAB63585.1.
AK058894 mRNA. No translation available.
AK059818 mRNA. No translation available.
AK099142 mRNA. No translation available.
AJ002381 mRNA. Translation: CAA05355.1.
PIRT03989.
RefSeqNP_001044339.1. NM_001050874.1.
UniGeneOs.2241.

3D structure databases

ProteinModelPortalO82451.
SMRO82451. Positions 2-215.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbO82451.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS01T0764000-01; OS01T0764000-01; OS01G0764000.
OS01T0764000-03; OS01T0764000-03; OS01G0764000.
GeneID4326887.
KEGGosa:4326887.

Organism-specific databases

GrameneO82451.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125746.
OMAWNVTRCV.
ProtClustDBCLSN2691874.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTF2_ORYSJ
AccessionPrimary (citable) accession number: O82451
Secondary accession number(s): O65858, Q7F5M8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations