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O82399

- MDHG2_ARATH

UniProt

O82399 - MDHG2_ARATH

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Protein

Probable malate dehydrogenase, glyoxysomal

Gene

At2g22780

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751NADBy similarity
Binding sitei122 – 1221SubstratePROSITE-ProRule annotation
Binding sitei128 – 1281SubstratePROSITE-ProRule annotation
Binding sitei135 – 1351NADBy similarity
Binding sitei160 – 1601SubstratePROSITE-ProRule annotation
Binding sitei194 – 1941SubstratePROSITE-ProRule annotation
Active sitei218 – 2181Proton acceptorBy similarity
Binding sitei269 – 2691NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 557NADBy similarity
Nucleotide bindingi158 – 1603NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. regulation of fatty acid beta-oxidation Source: TAIR
  4. regulation of photorespiration Source: TAIR
  5. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT2G22780-MONOMER.
MetaCyc:AT2G22780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
Gene namesi
Ordered Locus Names:At2g22780
ORF Names:T30L20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G22780.

Subcellular locationi

Glyoxysome Curated

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. glyoxysome Source: UniProtKB-KW
  3. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434GlyoxysomeSequence AnalysisAdd
BLAST
Chaini35 – 354320Probable malate dehydrogenase, glyoxysomalPRO_0000018635Add
BLAST

Proteomic databases

PaxDbiO82399.
PRIDEiO82399.

Expressioni

Gene expression databases

ExpressionAtlasiO82399. baseline and differential.
GenevestigatoriO82399.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi2161. 2 interactions.
IntActiO82399. 1 interaction.
STRINGi3702.AT2G22780.1-P.

Structurei

3D structure databases

ProteinModelPortaliO82399.
SMRiO82399. Positions 42-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiO82399.
KOiK00026.
OMAiDANIVEC.
PhylomeDBiO82399.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82399-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPNQRIARI SAHLNPPNLH NQIADGSGLN RVACRAKGGS PGFKVAILGA
60 70 80 90 100
AGGIGQPLAM LMKMNPLVSV LHLYDVANAP GVTADISHMD TSAVVRGFLG
110 120 130 140 150
QPQLEEALTG MDLVIIPAGV PRKPGMTRDD LFNINAGIVR TLSEAIAKCC
160 170 180 190 200
PKAIVNIISN PVNSTVPIAA EVFKKAGTFD PKKLMGVTML DVVRANTFVA
210 220 230 240 250
EVMSLDPREV EVPVVGGHAG VTILPLLSQV KPPCSFTQKE IEYLTDRIQN
260 270 280 290 300
GGTEVVEAKA GAGSATLSMA YAAVEFADAC LRGLRGDANI VECAYVASHV
310 320 330 340 350
TELPFFASKV RLGRCGIDEV YGLGPLNEYE RMGLEKAKKE LSVSIHKGVT

FAKK
Length:354
Mass (Da):37,466
Last modified:November 1, 1998 - v1
Checksum:i715CDC22B4448629
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005617 Genomic DNA. Translation: AAC63589.1.
CP002685 Genomic DNA. Translation: AEC07353.1.
AF428346 mRNA. Translation: AAL16276.1.
BT003009 mRNA. Translation: AAO23574.1.
PIRiG84616.
RefSeqiNP_179863.1. NM_127843.4.
UniGeneiAt.13158.

Genome annotation databases

EnsemblPlantsiAT2G22780.1; AT2G22780.1; AT2G22780.
GeneIDi816808.
KEGGiath:AT2G22780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005617 Genomic DNA. Translation: AAC63589.1 .
CP002685 Genomic DNA. Translation: AEC07353.1 .
AF428346 mRNA. Translation: AAL16276.1 .
BT003009 mRNA. Translation: AAO23574.1 .
PIRi G84616.
RefSeqi NP_179863.1. NM_127843.4.
UniGenei At.13158.

3D structure databases

ProteinModelPortali O82399.
SMRi O82399. Positions 42-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2161. 2 interactions.
IntActi O82399. 1 interaction.
STRINGi 3702.AT2G22780.1-P.

Proteomic databases

PaxDbi O82399.
PRIDEi O82399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G22780.1 ; AT2G22780.1 ; AT2G22780 .
GeneIDi 816808.
KEGGi ath:AT2G22780.

Organism-specific databases

TAIRi AT2G22780.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
InParanoidi O82399.
KOi K00026.
OMAi DANIVEC.
PhylomeDBi O82399.

Enzyme and pathway databases

BioCyci ARA:AT2G22780-MONOMER.
MetaCyc:AT2G22780-MONOMER.

Gene expression databases

ExpressionAtlasi O82399. baseline and differential.
Genevestigatori O82399.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiMDHG2_ARATH
AccessioniPrimary (citable) accession number: O82399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3