Reviewed, UniProtKB/Swiss-Prot O82399 (MDHG2_ARATH)

Names and origin

Protein names

Recommended name:
    Probable malate dehydrogenase, glyoxysomal
    EC=1.1.1.37

Gene names

Ordered Locus Names:	At2g22780
ORF Names:	T30L20.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Glyoxysome Potential.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of fatty acid beta-oxidation Inferred from genetic interaction. Source: TAIR regulation of photorespiration Inferred from mutant phenotype. Source: TAIR tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Glyoxysome Potential
	Chain	35 – 354	320	Probable malate dehydrogenase, glyoxysomal		PRO_0000018635
Regions
	Nucleotide binding	49 – 55	7	NAD By similarity
	Nucleotide binding	158 – 160	3	NAD By similarity
Sites
	Active site	218	1	Proton acceptor By similarity
	Binding site	75	1	NAD By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	135	1	NAD By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	269	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82399-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 715CDC22B4448629	FASTA	354	37,466
	10 20 30 40 50 60 MDPNQRIARI SAHLNPPNLH NQIADGSGLN RVACRAKGGS PGFKVAILGA AGGIGQPLAM 70 80 90 100 110 120 LMKMNPLVSV LHLYDVANAP GVTADISHMD TSAVVRGFLG QPQLEEALTG MDLVIIPAGV 130 140 150 160 170 180 PRKPGMTRDD LFNINAGIVR TLSEAIAKCC PKAIVNIISN PVNSTVPIAA EVFKKAGTFD 190 200 210 220 230 240 PKKLMGVTML DVVRANTFVA EVMSLDPREV EVPVVGGHAG VTILPLLSQV KPPCSFTQKE 250 260 270 280 290 300 IEYLTDRIQN GGTEVVEAKA GAGSATLSMA YAAVEFADAC LRGLRGDANI VECAYVASHV 310 320 330 340 350 TELPFFASKV RLGRCGIDEV YGLGPLNEYE RMGLEKAKKE LSVSIHKGVT FAKK

References

[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC005617 Genomic DNA. Translation: AAC63589.1. AF428346 mRNA. Translation: AAL16276.1. BT003009 mRNA. Translation: AAO23574.1.
IPI	IPI00523052.
PIR	G84616.
RefSeq	NP_179863.1.
UniGene	At.13158
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
SMR	O82399. Positions 42-353.
ModBase	Search...
Protein-protein interaction databases
IntAct	O82399. 1 interaction.
STRING	O82399.
Proteomic databases
PRIDE	O82399.
ProMEX	O82399.
Genome annotation databases
GeneID	816808.
GenomeReviews	Gene locus AT2G22780 in contig CT485783_GR.
KEGG	ath:AT2G22780.
Organism-specific databases
TAIR	At2g22780.
Phylogenomic databases
OMA	KVRLGRC.
Enzyme and pathway databases
BRENDA	1.1.1.37. 302.
Gene expression databases
ArrayExpress	O82399.
Genevestigator	O82399.
GermOnline	AT2G22780. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHG2_ARATH

Accession

Primary (citable) accession number: O82399

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)