Reviewed,
UniProtKB/Swiss-Prot O82399 (MDHG2_ARATH)
Last modified
November 25, 2008.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable malate dehydrogenase, glyoxysomal EC=1.1.1.37 | ||||
| Gene names |
| ||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 354 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD(+) = oxaloacetate + NADH. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | GlyoxysomePotential. |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 1 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Glyoxylate bypass Tricarboxylic acid cycle |
| Cellular component | Glyoxysome |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycleInferred from electronic annotation. Source: UniProtKB-KW malate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | glyoxysome Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 34 | 34 | Glyoxysome Potential | ||||||
| Chain | 35 – 354 | 320 | Probable malate dehydrogenase, glyoxysomal | PRO_0000018635 | |||||
Regions | |||||||||
| Nucleotide binding | 49 – 55 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 158 – 160 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 218 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 75 | 1 | NAD By similarity | ||||||
| Binding site | 122 | 1 | Substrate By similarity | ||||||
| Binding site | 128 | 1 | Substrate By similarity | ||||||
| Binding site | 135 | 1 | NAD By similarity | ||||||
| Binding site | 160 | 1 | Substrate By similarity | ||||||
| Binding site | 194 | 1 | Substrate By similarity | ||||||
| Binding site | 269 | 1 | NAD By similarity | ||||||
Sequences
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References
| [1] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| AC005617 Genomic DNA. Translation: AAC63589.1. AF428346 mRNA. Translation: AAL16276.1. BT003009 mRNA. Translation: AAO23574.1. | |
| PIR | G84616. |
| RefSeq | NP_179863.1. |
| UniGene | At.13158 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1MLD based on UniProtKB P00346. |
| SMR | O82399. Positions 42-353. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O82399. |
Proteomic databases | |
| ProMEX | O82399. |
Genome annotation databases | |
| GeneID | 816808. |
| GenomeReviews | Gene locus AT2G22780 in contig CT485783_GR. |
| KEGG | ath:AT2G22780. |
Organism-specific databases | |
| TAIR | At2g22780. |
Gene expression databases | |
| ArrayExpress | O82399. |
| GermOnline | AT2G22780. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010097. Malate_DHase_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR11540:SF1. MDH_euk_g_bac. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| TIGRFAMs | TIGR01772. MDH_euk_gproteo. 1 hit. |
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MDHG2_ARATH | ||||||||
| Accession | Primary (citable) accession number: O82399 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


