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O82391

- JOSL_ARATH

UniProt

O82391 - JOSL_ARATH

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Protein

Josephin-like protein

Gene
At2g29640, T27A16.26
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

May act as a deubiquitinating enzyme By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181Nucleophile By similarity
Active sitei129 – 1291Proton acceptor By similarity

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC86.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-like protein (EC:3.4.19.12)
Gene namesi
Ordered Locus Names:At2g29640
ORF Names:T27A16.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G29640.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Josephin-like proteinPRO_0000053846Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriO82391.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29640.1-P.

Structurei

3D structure databases

ProteinModelPortaliO82391.
SMRiO82391. Positions 8-155.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 192188JosephinAdd
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.

Phylogenomic databases

eggNOGiNOG321724.
HOGENOMiHOG000113135.
InParanoidiO82391.
KOiK15235.
OMAiRECWIDY.
PhylomeDBiO82391.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82391-1 [UniParc]FASTAAdd to Basket

« Hide

MADSESKIYH ERQRLQFCLL HCLNNLFQDK DAFTKESLNS IAEKLETNDP    50
NKETWTPLSF VLKPHHNTIT GNYDVNVMIT ALEGKGKSVV WHDKRIGASS 100
IDLDDADTLM GIVLNVPVKR YGGLWRSRHW VVVRKINGVW YNLDSDLVVP 150
QLFRDDDEVR GFLDQNLSLD VIGLIWNLPY NANQLPLASN YRVVITHWSD 200
LNGMFSHVTN YPLDFDVYPQ VSSKVSAIYK ATGTKRFNAN TRPVTPGKQS 250
SVKGPYYKNP GCTTSCGLRL PRKTECTAAR LIKDLSCKFV MGLRLVVMRK 300
KKKKRSPPLK KASSSGISQP SVISVVNDNN HRSAAIEDCI QFINSSSSFT 350
RSNSTCGSKS 360
Length:360
Mass (Da):40,489
Last modified:November 1, 1998 - v1
Checksum:i01701E35AB2C981D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005496 Genomic DNA. Translation: AAC35231.1.
CP002685 Genomic DNA. Translation: AEC08284.1.
PIRiG84698.
RefSeqiNP_180525.1. NM_128518.1.
UniGeneiAt.52967.

Genome annotation databases

EnsemblPlantsiAT2G29640.1; AT2G29640.1; AT2G29640.
GeneIDi817514.
KEGGiath:AT2G29640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005496 Genomic DNA. Translation: AAC35231.1 .
CP002685 Genomic DNA. Translation: AEC08284.1 .
PIRi G84698.
RefSeqi NP_180525.1. NM_128518.1.
UniGenei At.52967.

3D structure databases

ProteinModelPortali O82391.
SMRi O82391. Positions 8-155.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G29640.1-P.

Protein family/group databases

MEROPSi C86.A02.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G29640.1 ; AT2G29640.1 ; AT2G29640 .
GeneIDi 817514.
KEGGi ath:AT2G29640.

Organism-specific databases

TAIRi AT2G29640.

Phylogenomic databases

eggNOGi NOG321724.
HOGENOMi HOG000113135.
InParanoidi O82391.
KOi K15235.
OMAi RECWIDY.
PhylomeDBi O82391.

Miscellaneous databases

PROi O82391.

Gene expression databases

Genevestigatori O82391.

Family and domain databases

InterProi IPR006155. Josephin.
[Graphical view ]
Pfami PF02099. Josephin. 1 hit.
[Graphical view ]
PROSITEi PS50957. JOSEPHIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
    Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
    Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiJOSL_ARATH
AccessioniPrimary (citable) accession number: O82391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3