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Protein

Josephin-like protein

Gene

At2g29640

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

May act as a deubiquitinating enzyme.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181NucleophilePROSITE-ProRule annotation
Active sitei129 – 1291Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Nucleotide-binding

Protein family/group databases

MEROPSiC86.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-like protein (EC:3.4.19.12)
Gene namesi
Ordered Locus Names:At2g29640
ORF Names:T27A16.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29640.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Josephin-like proteinPRO_0000053846Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29640.1.

Structurei

3D structure databases

ProteinModelPortaliO82391.
SMRiO82391. Positions 8-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 192188JosephinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG321724.
HOGENOMiHOG000113135.
InParanoidiO82391.
KOiK15235.
OMAiRFNANTR.
PhylomeDBiO82391.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSESKIYH ERQRLQFCLL HCLNNLFQDK DAFTKESLNS IAEKLETNDP
60 70 80 90 100
NKETWTPLSF VLKPHHNTIT GNYDVNVMIT ALEGKGKSVV WHDKRIGASS
110 120 130 140 150
IDLDDADTLM GIVLNVPVKR YGGLWRSRHW VVVRKINGVW YNLDSDLVVP
160 170 180 190 200
QLFRDDDEVR GFLDQNLSLD VIGLIWNLPY NANQLPLASN YRVVITHWSD
210 220 230 240 250
LNGMFSHVTN YPLDFDVYPQ VSSKVSAIYK ATGTKRFNAN TRPVTPGKQS
260 270 280 290 300
SVKGPYYKNP GCTTSCGLRL PRKTECTAAR LIKDLSCKFV MGLRLVVMRK
310 320 330 340 350
KKKKRSPPLK KASSSGISQP SVISVVNDNN HRSAAIEDCI QFINSSSSFT
360
RSNSTCGSKS
Length:360
Mass (Da):40,489
Last modified:November 1, 1998 - v1
Checksum:i01701E35AB2C981D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35231.1.
CP002685 Genomic DNA. Translation: AEC08284.1.
PIRiG84698.
RefSeqiNP_180525.1. NM_128518.1.
UniGeneiAt.52967.

Genome annotation databases

EnsemblPlantsiAT2G29640.1; AT2G29640.1; AT2G29640.
GeneIDi817514.
KEGGiath:AT2G29640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35231.1.
CP002685 Genomic DNA. Translation: AEC08284.1.
PIRiG84698.
RefSeqiNP_180525.1. NM_128518.1.
UniGeneiAt.52967.

3D structure databases

ProteinModelPortaliO82391.
SMRiO82391. Positions 8-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G29640.1.

Protein family/group databases

MEROPSiC86.A02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29640.1; AT2G29640.1; AT2G29640.
GeneIDi817514.
KEGGiath:AT2G29640.

Organism-specific databases

TAIRiAT2G29640.

Phylogenomic databases

eggNOGiNOG321724.
HOGENOMiHOG000113135.
InParanoidiO82391.
KOiK15235.
OMAiRFNANTR.
PhylomeDBiO82391.

Miscellaneous databases

PROiO82391.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
    Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
    Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiJOSL_ARATH
AccessioniPrimary (citable) accession number: O82391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.