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O82391

- JOSL_ARATH

UniProt

O82391 - JOSL_ARATH

Protein

Josephin-like protein

Gene

At2g29640

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    May act as a deubiquitinating enzyme.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei18 – 181NucleophilePROSITE-ProRule annotation
    Active sitei129 – 1291Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. omega peptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Nucleotide-binding

    Protein family/group databases

    MEROPSiC86.A02.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Josephin-like protein (EC:3.4.19.12)
    Gene namesi
    Ordered Locus Names:At2g29640
    ORF Names:T27A16.26
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G29640.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Josephin-like proteinPRO_0000053846Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriO82391.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G29640.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82391.
    SMRiO82391. Positions 8-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 192188JosephinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Josephin domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG321724.
    HOGENOMiHOG000113135.
    InParanoidiO82391.
    KOiK15235.
    OMAiRECWIDY.
    PhylomeDBiO82391.

    Family and domain databases

    InterProiIPR006155. Josephin.
    [Graphical view]
    PfamiPF02099. Josephin. 1 hit.
    [Graphical view]
    PROSITEiPS50957. JOSEPHIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O82391-1 [UniParc]FASTAAdd to Basket

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    MADSESKIYH ERQRLQFCLL HCLNNLFQDK DAFTKESLNS IAEKLETNDP    50
    NKETWTPLSF VLKPHHNTIT GNYDVNVMIT ALEGKGKSVV WHDKRIGASS 100
    IDLDDADTLM GIVLNVPVKR YGGLWRSRHW VVVRKINGVW YNLDSDLVVP 150
    QLFRDDDEVR GFLDQNLSLD VIGLIWNLPY NANQLPLASN YRVVITHWSD 200
    LNGMFSHVTN YPLDFDVYPQ VSSKVSAIYK ATGTKRFNAN TRPVTPGKQS 250
    SVKGPYYKNP GCTTSCGLRL PRKTECTAAR LIKDLSCKFV MGLRLVVMRK 300
    KKKKRSPPLK KASSSGISQP SVISVVNDNN HRSAAIEDCI QFINSSSSFT 350
    RSNSTCGSKS 360
    Length:360
    Mass (Da):40,489
    Last modified:November 1, 1998 - v1
    Checksum:i01701E35AB2C981D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005496 Genomic DNA. Translation: AAC35231.1.
    CP002685 Genomic DNA. Translation: AEC08284.1.
    PIRiG84698.
    RefSeqiNP_180525.1. NM_128518.1.
    UniGeneiAt.52967.

    Genome annotation databases

    EnsemblPlantsiAT2G29640.1; AT2G29640.1; AT2G29640.
    GeneIDi817514.
    KEGGiath:AT2G29640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005496 Genomic DNA. Translation: AAC35231.1 .
    CP002685 Genomic DNA. Translation: AEC08284.1 .
    PIRi G84698.
    RefSeqi NP_180525.1. NM_128518.1.
    UniGenei At.52967.

    3D structure databases

    ProteinModelPortali O82391.
    SMRi O82391. Positions 8-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G29640.1-P.

    Protein family/group databases

    MEROPSi C86.A02.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G29640.1 ; AT2G29640.1 ; AT2G29640 .
    GeneIDi 817514.
    KEGGi ath:AT2G29640.

    Organism-specific databases

    TAIRi AT2G29640.

    Phylogenomic databases

    eggNOGi NOG321724.
    HOGENOMi HOG000113135.
    InParanoidi O82391.
    KOi K15235.
    OMAi RECWIDY.
    PhylomeDBi O82391.

    Miscellaneous databases

    PROi O82391.

    Gene expression databases

    Genevestigatori O82391.

    Family and domain databases

    InterProi IPR006155. Josephin.
    [Graphical view ]
    Pfami PF02099. Josephin. 1 hit.
    [Graphical view ]
    PROSITEi PS50957. JOSEPHIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
      Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
      Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiJOSL_ARATH
    AccessioniPrimary (citable) accession number: O82391
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3