O82391 (JOSL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Josephin-like protein
EC=3.4.19.12

Gene names

Ordered Locus Names:	At2g29640
ORF Names:	T27A16.26

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May act as a deubiquitinating enzyme By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 Josephin domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	Nucleotide-binding
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW omega peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Josephin-like protein		PRO_0000053846
Regions
	Domain	5 – 192	188	Josephin
Sites
	Active site	18	1	Nucleophile By similarity
	Active site	129	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82391 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 01701E35AB2C981D	FASTA	360	40,489
	10 20 30 40 50 60 MADSESKIYH ERQRLQFCLL HCLNNLFQDK DAFTKESLNS IAEKLETNDP NKETWTPLSF 70 80 90 100 110 120 VLKPHHNTIT GNYDVNVMIT ALEGKGKSVV WHDKRIGASS IDLDDADTLM GIVLNVPVKR 130 140 150 160 170 180 YGGLWRSRHW VVVRKINGVW YNLDSDLVVP QLFRDDDEVR GFLDQNLSLD VIGLIWNLPY 190 200 210 220 230 240 NANQLPLASN YRVVITHWSD LNGMFSHVTN YPLDFDVYPQ VSSKVSAIYK ATGTKRFNAN 250 260 270 280 290 300 TRPVTPGKQS SVKGPYYKNP GCTTSCGLRL PRKTECTAAR LIKDLSCKFV MGLRLVVMRK 310 320 330 340 350 360 KKKKRSPPLK KASSSGISQP SVISVVNDNN HRSAAIEDCI QFINSSSSFT RSNSTCGSKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Structural modeling of ataxin-3 reveals distant homology to adaptins." Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T. Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005496 Genomic DNA. Translation: AAC35231.1. CP002685 Genomic DNA. Translation: AEC08284.1.
IPI	IPI00532380.
PIR	G84698.
RefSeq	NP_180525.1. NM_128518.1.
UniGene	At.52967.
3D structure databases
ProteinModelPortal	O82391.
SMR	O82391. Positions 8-155.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G29640.1-P.
Protein family/group databases
MEROPS	C86.A02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29640.1; AT2G29640.1; AT2G29640.
GeneID	817514.
KEGG	ath:AT2G29640.
Organism-specific databases
TAIR	At2g29640.
Phylogenomic databases
eggNOG	NOG321724.
HOGENOM	HOG000113135.
InParanoid	O82391.
KO	K15235.
OMA	RFNANTR.
PhylomeDB	O82391.
ProtClustDB	CLSN2913264.
Gene expression databases
Genevestigator	O82391.
Family and domain databases
InterPro	IPR006155. Josephin. [Graphical view]
Pfam	PF02099. Josephin. 1 hit. [Graphical view]
PROSITE	PS50957. JOSEPHIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JOSL_ARATH

Accession

Primary (citable) accession number: O82391

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program