O82383 (U71D1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 71D1
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71D1
EC=2.4.1.91

Gene names

Name:	UGT71D1
Ordered Locus Names:	At2g29730
ORF Names:	T27A16.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase activity in vitro. Ref.6
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	UDP-glycosyltransferase 71D1		PRO_0000409058
Regions
	Region	339 – 341	3	UDP-glucose binding By similarity
	Region	356 – 364	9	UDP-glucose binding By similarity
	Region	378 – 381	4	UDP-glucose binding By similarity
Sites
	Binding site	284	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82383 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 57AEA8A8058CA8E7	FASTA	467	53,000
	10 20 30 40 50 60 MRNVELIFIP TPTVGHLVPF LEFARRLIEQ DDRIRITILL MKLQGQSHLD TYVKSIASSQ 70 80 90 100 110 120 PFVRFIDVPE LEEKPTLGST QSVEAYVYDV IERNIPLVRN IVMDILTSLA LDGVKVKGLV 130 140 150 160 170 180 VDFFCLPMID VAKDISLPFY VFLTTNSGFL AMMQYLADRH SRDTSVFVRN SEEMLSIPGF 190 200 210 220 230 240 VNPVPANVLP SALFVEDGYD AYVKLAILFT KANGILVNSS FDIEPYSVNH FLQEQNYPSV 250 260 270 280 290 300 YAVGPIFDLK AQPHPEQDLT RRDELMKWLD DQPEASVVFL CFGSMARLRG SLVKEIAHGL 310 320 330 340 350 360 ELCQYRFLWS LRKEEVTKDD LPEGFLDRVD GRGMICGWSP QVEILAHKAV GGFVSHCGWN 370 380 390 400 410 420 SIVESLWFGV PIVTWPMYAE QQLNAFLMVK ELKLAVELKL DYRVHSDEIV NANEIETAIR 430 440 450 460 YVMDTDNNVV RKRVMDISQM IQRATKNGGS SFAAIEKFIY DVIGIKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005496 Genomic DNA. Translation: AAC35239.1. CP002685 Genomic DNA. Translation: AEC08298.1. AY099557 mRNA. Translation: AAM20409.1. BT006599 mRNA. Translation: AAP31943.1. AY086939 mRNA. Translation: AAM64503.1.
IPI	IPI00535402.
PIR	H84699.
RefSeq	NP_180534.1. NM_128527.3.
UniGene	At.27562.
3D structure databases
ProteinModelPortal	O82383.
SMR	O82383. Positions 2-464.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G29730.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	O82383.
PRIDE	O82383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29730.1; AT2G29730.1; AT2G29730.
GeneID	817523.
KEGG	ath:AT2G29730.
Organism-specific databases
TAIR	At2g29730.
Phylogenomic databases
eggNOG	NOG277278.
HOGENOM	HOG000237568.
InParanoid	O82383.
OMA	VELIFIP.
PhylomeDB	O82383.
ProtClustDB	PLN02207.
Gene expression databases
Genevestigator	O82383.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U71D1_ARATH

Accession

Primary (citable) accession number: O82383

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program