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O82381

- U71C1_ARATH

UniProt

O82381 - U71C1_ARATH

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Protein

UDP-glycosyltransferase 71C1

Gene

UGT71C1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide.3 Publications

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

Kineticsi

  1. KM=0.15 mM for curcumin1 Publication
  2. KM=0.5 mM for trans-resveratrol1 Publication
  3. KM=1.12 mM for vanillin1 Publication
  4. KM=2.7 mM for etoposide1 Publication

Vmax=1.2 µmol/min/mg enzyme towards vanillin1 Publication

Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol1 Publication

Vmax=0.022 µmol/min/mg enzyme towards curcumin1 Publication

Vmax=0.013 µmol/min/mg enzyme towards etoposide1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei293 – 2931UDP-glucoseBy similarity

GO - Molecular functioni

  1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
  2. quercetin 3'-O-glucosyltransferase activity Source: TAIR
  3. quercetin 7-O-glucosyltransferase activity Source: TAIR
  4. UDP-glucosyltransferase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

SABIO-RKO82381.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 71C1 (EC:2.4.1.-)
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C1 (EC:2.4.1.91)
Flavonol 7-O-glucosyltransferase UGT71C1
Gene namesi
Name:UGT71C1
Ordered Locus Names:At2g29750
ORF Names:T27A16.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G29750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481UDP-glycosyltransferase 71C1PRO_0000409053Add
BLAST

Proteomic databases

PaxDbiO82381.
PRIDEiO82381.

Expressioni

Gene expression databases

GenevestigatoriO82381.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29750.1-P.

Structurei

3D structure databases

ProteinModelPortaliO82381.
SMRiO82381. Positions 9-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni352 – 3543UDP-glucose bindingBy similarity
Regioni369 – 3779UDP-glucose bindingBy similarity
Regioni391 – 3944UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiNOG326467.
HOGENOMiHOG000237568.
InParanoidiO82381.
OMAiPRIHTIT.
PhylomeDBiO82381.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82381-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF
60 70 80 90 100
IPQADTIAFL RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK
110 120 130 140 150
KMVPIIREAL STLLSSRDES GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI
160 170 180 190 200
FLTCSAGFLG MMKYLPERHR EIKSEFNRSF NEELNLIPGY VNSVPTKVLP
210 220 230 240 250
SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY FDRCPDNYPT
260 270 280 290 300
IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT
310 320 330 340 350
QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG
360 370 380 390 400
WAPQVEILAH KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT
410 420 430 440 450
MVKELGLALE MRLDYVSEDG DIVKADEIAG TVRSLMDGVD VPKSKVKEIA
460 470 480
EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS K
Length:481
Mass (Da):53,875
Last modified:November 1, 1998 - v1
Checksum:i21283D9B69C4C97C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35226.1.
CP002685 Genomic DNA. Translation: AEC08300.1.
BT023426 mRNA. Translation: AAY56417.1.
BT026458 mRNA. Translation: ABH04565.1.
PIRiB84700.
RefSeqiNP_180536.1. NM_128529.2.
UniGeneiAt.13110.

Genome annotation databases

EnsemblPlantsiAT2G29750.1; AT2G29750.1; AT2G29750.
GeneIDi817525.
KEGGiath:AT2G29750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35226.1 .
CP002685 Genomic DNA. Translation: AEC08300.1 .
BT023426 mRNA. Translation: AAY56417.1 .
BT026458 mRNA. Translation: ABH04565.1 .
PIRi B84700.
RefSeqi NP_180536.1. NM_128529.2.
UniGenei At.13110.

3D structure databases

ProteinModelPortali O82381.
SMRi O82381. Positions 9-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G29750.1-P.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbi O82381.
PRIDEi O82381.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G29750.1 ; AT2G29750.1 ; AT2G29750 .
GeneIDi 817525.
KEGGi ath:AT2G29750.

Organism-specific databases

TAIRi AT2G29750.

Phylogenomic databases

eggNOGi NOG326467.
HOGENOMi HOG000237568.
InParanoidi O82381.
OMAi PRIHTIT.
PhylomeDBi O82381.

Enzyme and pathway databases

SABIO-RK O82381.

Gene expression databases

Genevestigatori O82381.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF Clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
    Li Y., Baldauf S., Lim E.K., Bowles D.J.
    J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
    Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
    J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
    Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
    Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Substrate specificities of family 1 UGTs gained by domain swapping."
    Hansen E.H., Osmani S.A., Kristensen C., Moeller B.L., Hansen J.
    Phytochemistry 70:473-482(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiU71C1_ARATH
AccessioniPrimary (citable) accession number: O82381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: November 1, 1998
Last modified: October 1, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3