O82381 (U71C1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 71C1
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C1
EC=2.4.1.91
Flavonol 7-O-glucosyltransferase UGT71C1

Gene names

Name:	UGT71C1
Ordered Locus Names:	At2g29750
ORF Names:	T27A16.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide. Ref.6 Ref.7 Ref.8
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside. UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=0.15 mM for curcumin Ref.8 KM=0.5 mM for trans-resveratrol KM=1.12 mM for vanillin KM=2.7 mM for etoposide Vmax=1.2 µmol/min/mg enzyme towards vanillin Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol Vmax=0.022 µmol/min/mg enzyme towards curcumin Vmax=0.013 µmol/min/mg enzyme towards etoposide

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3'-O-glucosyltransferase activity Inferred from direct assay Ref.7. Source: TAIR quercetin 7-O-glucosyltransferase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	UDP-glycosyltransferase 71C1		PRO_0000409053
Regions
	Region	352 – 354	3	UDP-glucose binding By similarity
	Region	369 – 377	9	UDP-glucose binding By similarity
	Region	391 – 394	4	UDP-glucose binding By similarity
Sites
	Binding site	293	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	O82381 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 21283D9B69C4C97C	FASTA	481	53,875
	10 20 30 40 50 60 MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF IPQADTIAFL 70 80 90 100 110 120 RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK KMVPIIREAL STLLSSRDES 130 140 150 160 170 180 GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI FLTCSAGFLG MMKYLPERHR EIKSEFNRSF 190 200 210 220 230 240 NEELNLIPGY VNSVPTKVLP SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY 250 260 270 280 290 300 FDRCPDNYPT IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT 310 320 330 340 350 360 QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG WAPQVEILAH 370 380 390 400 410 420 KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT MVKELGLALE MRLDYVSEDG 430 440 450 460 470 480 DIVKADEIAG TVRSLMDGVD VPKSKVKEIA EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF Clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates." Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J. J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Substrate specificities of family 1 UGTs gained by domain swapping." Hansen E.H., Osmani S.A., Kristensen C., Moeller B.L., Hansen J. Phytochemistry 70:473-482(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005496 Genomic DNA. Translation: AAC35226.1. CP002685 Genomic DNA. Translation: AEC08300.1. BT023426 mRNA. Translation: AAY56417.1. BT026458 mRNA. Translation: ABH04565.1.
IPI	IPI00533972.
PIR	B84700.
RefSeq	NP_180536.1. NM_128529.2.
UniGene	At.13110.
3D structure databases
ProteinModelPortal	O82381.
SMR	O82381. Positions 9-466.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G29750.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	O82381.
PRIDE	O82381.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29750.1; AT2G29750.1; AT2G29750.
GeneID	817525.
KEGG	ath:AT2G29750.
Organism-specific databases
TAIR	At2g29750.
Phylogenomic databases
eggNOG	NOG326467.
HOGENOM	HOG000237568.
InParanoid	O82381.
OMA	PRIHTIT.
PhylomeDB	O82381.
ProtClustDB	PLN02167.
Gene expression databases
Genevestigator	O82381.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U71C1_ARATH

Accession

Primary (citable) accession number: O82381

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program