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Protein

UDP-glycosyltransferase 71C1

Gene

UGT71C1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide.3 Publications

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

Kineticsi

  1. KM=0.15 mM for curcumin1 Publication
  2. KM=0.5 mM for trans-resveratrol1 Publication
  3. KM=1.12 mM for vanillin1 Publication
  4. KM=2.7 mM for etoposide1 Publication

Vmax=1.2 µmol/min/mg enzyme towards vanillin1 Publication

Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol1 Publication

Vmax=0.022 µmol/min/mg enzyme towards curcumin1 Publication

Vmax=0.013 µmol/min/mg enzyme towards etoposide1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei293 – 2931UDP-glucoseBy similarity

GO - Molecular functioni

  1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
  2. quercetin 3'-O-glucosyltransferase activity Source: TAIR
  3. quercetin 3-O-glucosyltransferase activity Source: GO_Central
  4. quercetin 7-O-glucosyltransferase activity Source: TAIR
  5. UDP-glucosyltransferase activity Source: TAIR

GO - Biological processi

  1. flavonoid biosynthetic process Source: GO_Central
  2. flavonoid glucuronidation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.128. 399.
SABIO-RKO82381.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 71C1 (EC:2.4.1.-)
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C1 (EC:2.4.1.91)
Flavonol 7-O-glucosyltransferase UGT71C1
Gene namesi
Name:UGT71C1
Ordered Locus Names:At2g29750
ORF Names:T27A16.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29750.

Subcellular locationi

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481UDP-glycosyltransferase 71C1PRO_0000409053Add
BLAST

Proteomic databases

PaxDbiO82381.
PRIDEiO82381.

Expressioni

Gene expression databases

ExpressionAtlasiO82381. baseline and differential.
GenevestigatoriO82381.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29750.1-P.

Structurei

3D structure databases

SMRiO82381. Positions 9-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni352 – 3543UDP-glucose bindingBy similarity
Regioni369 – 3779UDP-glucose bindingBy similarity
Regioni391 – 3944UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiNOG326467.
HOGENOMiHOG000237568.
InParanoidiO82381.
OMAiKEYASPY.
PhylomeDBiO82381.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF
60 70 80 90 100
IPQADTIAFL RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK
110 120 130 140 150
KMVPIIREAL STLLSSRDES GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI
160 170 180 190 200
FLTCSAGFLG MMKYLPERHR EIKSEFNRSF NEELNLIPGY VNSVPTKVLP
210 220 230 240 250
SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY FDRCPDNYPT
260 270 280 290 300
IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT
310 320 330 340 350
QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG
360 370 380 390 400
WAPQVEILAH KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT
410 420 430 440 450
MVKELGLALE MRLDYVSEDG DIVKADEIAG TVRSLMDGVD VPKSKVKEIA
460 470 480
EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS K
Length:481
Mass (Da):53,875
Last modified:November 1, 1998 - v1
Checksum:i21283D9B69C4C97C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35226.1.
CP002685 Genomic DNA. Translation: AEC08300.1.
BT023426 mRNA. Translation: AAY56417.1.
BT026458 mRNA. Translation: ABH04565.1.
PIRiB84700.
RefSeqiNP_180536.1. NM_128529.2.
UniGeneiAt.13110.

Genome annotation databases

EnsemblPlantsiAT2G29750.1; AT2G29750.1; AT2G29750.
GeneIDi817525.
KEGGiath:AT2G29750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA. Translation: AAC35226.1.
CP002685 Genomic DNA. Translation: AEC08300.1.
BT023426 mRNA. Translation: AAY56417.1.
BT026458 mRNA. Translation: ABH04565.1.
PIRiB84700.
RefSeqiNP_180536.1. NM_128529.2.
UniGeneiAt.13110.

3D structure databases

SMRiO82381. Positions 9-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G29750.1-P.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiO82381.
PRIDEiO82381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29750.1; AT2G29750.1; AT2G29750.
GeneIDi817525.
KEGGiath:AT2G29750.

Organism-specific databases

TAIRiAT2G29750.

Phylogenomic databases

eggNOGiNOG326467.
HOGENOMiHOG000237568.
InParanoidiO82381.
OMAiKEYASPY.
PhylomeDBiO82381.

Enzyme and pathway databases

BRENDAi2.4.1.128. 399.
SABIO-RKO82381.

Miscellaneous databases

PROiO82381.

Gene expression databases

ExpressionAtlasiO82381. baseline and differential.
GenevestigatoriO82381.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF Clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
    Li Y., Baldauf S., Lim E.K., Bowles D.J.
    J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
    Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
    J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
    Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
    Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Substrate specificities of family 1 UGTs gained by domain swapping."
    Hansen E.H., Osmani S.A., Kristensen C., Moeller B.L., Hansen J.
    Phytochemistry 70:473-482(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiU71C1_ARATH
AccessioniPrimary (citable) accession number: O82381
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: November 1, 1998
Last modified: April 29, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.