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O82381

- U71C1_ARATH

UniProt

O82381 - U71C1_ARATH

Protein

UDP-glycosyltransferase 71C1

Gene

UGT71C1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide.3 Publications

    Catalytic activityi

    UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
    UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

    Kineticsi

    1. KM=0.15 mM for curcumin1 Publication
    2. KM=0.5 mM for trans-resveratrol1 Publication
    3. KM=1.12 mM for vanillin1 Publication
    4. KM=2.7 mM for etoposide1 Publication

    Vmax=1.2 µmol/min/mg enzyme towards vanillin1 Publication

    Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol1 Publication

    Vmax=0.022 µmol/min/mg enzyme towards curcumin1 Publication

    Vmax=0.013 µmol/min/mg enzyme towards etoposide1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei293 – 2931UDP-glucoseBy similarity

    GO - Molecular functioni

    1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
    2. quercetin 3'-O-glucosyltransferase activity Source: TAIR
    3. quercetin 7-O-glucosyltransferase activity Source: TAIR
    4. UDP-glucosyltransferase activity Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    SABIO-RKO82381.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glycosyltransferase 71C1 (EC:2.4.1.-)
    Alternative name(s):
    Flavonol 3-O-glucosyltransferase UGT71C1 (EC:2.4.1.91)
    Flavonol 7-O-glucosyltransferase UGT71C1
    Gene namesi
    Name:UGT71C1
    Ordered Locus Names:At2g29750
    ORF Names:T27A16.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G29750.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 481481UDP-glycosyltransferase 71C1PRO_0000409053Add
    BLAST

    Proteomic databases

    PaxDbiO82381.
    PRIDEiO82381.

    Expressioni

    Gene expression databases

    GenevestigatoriO82381.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G29750.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82381.
    SMRiO82381. Positions 9-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni352 – 3543UDP-glucose bindingBy similarity
    Regioni369 – 3779UDP-glucose bindingBy similarity
    Regioni391 – 3944UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiNOG326467.
    HOGENOMiHOG000237568.
    InParanoidiO82381.
    OMAiPRIHTIT.
    PhylomeDBiO82381.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O82381-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF    50
    IPQADTIAFL RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK 100
    KMVPIIREAL STLLSSRDES GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI 150
    FLTCSAGFLG MMKYLPERHR EIKSEFNRSF NEELNLIPGY VNSVPTKVLP 200
    SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY FDRCPDNYPT 250
    IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT 300
    QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG 350
    WAPQVEILAH KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT 400
    MVKELGLALE MRLDYVSEDG DIVKADEIAG TVRSLMDGVD VPKSKVKEIA 450
    EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS K 481
    Length:481
    Mass (Da):53,875
    Last modified:November 1, 1998 - v1
    Checksum:i21283D9B69C4C97C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005496 Genomic DNA. Translation: AAC35226.1.
    CP002685 Genomic DNA. Translation: AEC08300.1.
    BT023426 mRNA. Translation: AAY56417.1.
    BT026458 mRNA. Translation: ABH04565.1.
    PIRiB84700.
    RefSeqiNP_180536.1. NM_128529.2.
    UniGeneiAt.13110.

    Genome annotation databases

    EnsemblPlantsiAT2G29750.1; AT2G29750.1; AT2G29750.
    GeneIDi817525.
    KEGGiath:AT2G29750.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005496 Genomic DNA. Translation: AAC35226.1 .
    CP002685 Genomic DNA. Translation: AEC08300.1 .
    BT023426 mRNA. Translation: AAY56417.1 .
    BT026458 mRNA. Translation: ABH04565.1 .
    PIRi B84700.
    RefSeqi NP_180536.1. NM_128529.2.
    UniGenei At.13110.

    3D structure databases

    ProteinModelPortali O82381.
    SMRi O82381. Positions 9-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G29750.1-P.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    Proteomic databases

    PaxDbi O82381.
    PRIDEi O82381.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G29750.1 ; AT2G29750.1 ; AT2G29750 .
    GeneIDi 817525.
    KEGGi ath:AT2G29750.

    Organism-specific databases

    TAIRi AT2G29750.

    Phylogenomic databases

    eggNOGi NOG326467.
    HOGENOMi HOG000237568.
    InParanoidi O82381.
    OMAi PRIHTIT.
    PhylomeDBi O82381.

    Enzyme and pathway databases

    SABIO-RK O82381.

    Gene expression databases

    Genevestigatori O82381.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
      Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF Clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
      Li Y., Baldauf S., Lim E.K., Bowles D.J.
      J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
      Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
      J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
      Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
      Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Substrate specificities of family 1 UGTs gained by domain swapping."
      Hansen E.H., Osmani S.A., Kristensen C., Moeller B.L., Hansen J.
      Phytochemistry 70:473-482(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiU71C1_ARATH
    AccessioniPrimary (citable) accession number: O82381
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3