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O82381 (U71C1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glycosyltransferase 71C1

EC=2.4.1.-
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C1
EC=2.4.1.91
Flavonol 7-O-glucosyltransferase UGT71C1
Gene names
Name:UGT71C1
Ordered Locus Names:At2g29750
ORF Names:T27A16.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide. Ref.6 Ref.7 Ref.8

Catalytic activity

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.15 mM for curcumin Ref.8

KM=0.5 mM for trans-resveratrol

KM=1.12 mM for vanillin

KM=2.7 mM for etoposide

Vmax=1.2 µmol/min/mg enzyme towards vanillin

Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol

Vmax=0.022 µmol/min/mg enzyme towards curcumin

Vmax=0.013 µmol/min/mg enzyme towards etoposide

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481UDP-glycosyltransferase 71C1
PRO_0000409053

Regions

Region352 – 3543UDP-glucose binding By similarity
Region369 – 3779UDP-glucose binding By similarity
Region391 – 3944UDP-glucose binding By similarity

Sites

Binding site2931UDP-glucose By similarity

Sequences

Sequence LengthMass (Da)Tools
O82381 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 21283D9B69C4C97C

FASTA48153,875
        10         20         30         40         50         60 
MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF IPQADTIAFL 

        70         80         90        100        110        120 
RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK KMVPIIREAL STLLSSRDES 

       130        140        150        160        170        180 
GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI FLTCSAGFLG MMKYLPERHR EIKSEFNRSF 

       190        200        210        220        230        240 
NEELNLIPGY VNSVPTKVLP SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY 

       250        260        270        280        290        300 
FDRCPDNYPT IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT 

       310        320        330        340        350        360 
QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG WAPQVEILAH 

       370        380        390        400        410        420 
KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT MVKELGLALE MRLDYVSEDG 

       430        440        450        460        470        480 
DIVKADEIAG TVRSLMDGVD VPKSKVKEIA EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS 


K 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF Clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
Li Y., Baldauf S., Lim E.K., Bowles D.J.
J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Substrate specificities of family 1 UGTs gained by domain swapping."
Hansen E.H., Osmani S.A., Kristensen C., Moeller B.L., Hansen J.
Phytochemistry 70:473-482(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005496 Genomic DNA. Translation: AAC35226.1.
CP002685 Genomic DNA. Translation: AEC08300.1.
BT023426 mRNA. Translation: AAY56417.1.
BT026458 mRNA. Translation: ABH04565.1.
PIRB84700.
RefSeqNP_180536.1. NM_128529.2.
UniGeneAt.13110.

3D structure databases

ProteinModelPortalO82381.
SMRO82381. Positions 9-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G29750.1-P.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbO82381.
PRIDEO82381.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G29750.1; AT2G29750.1; AT2G29750.
GeneID817525.
KEGGath:AT2G29750.

Organism-specific databases

TAIRAT2G29750.

Phylogenomic databases

eggNOGNOG326467.
HOGENOMHOG000237568.
InParanoidO82381.
OMAPRIHTIT.
PhylomeDBO82381.

Enzyme and pathway databases

SABIO-RKO82381.

Gene expression databases

GenevestigatorO82381.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameU71C1_ARATH
AccessionPrimary (citable) accession number: O82381
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names