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Protein

UDP-glycosyltransferase 71C1

Gene

UGT71C1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Glucosylates other secondary metabolites in vitro like trans-resveratrol, curcumin, vanillin and etoposide.3 Publications

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.

Kineticsi

  1. KM=0.15 mM for curcumin1 Publication
  2. KM=0.5 mM for trans-resveratrol1 Publication
  3. KM=1.12 mM for vanillin1 Publication
  4. KM=2.7 mM for etoposide1 Publication
  1. Vmax=1.2 µmol/min/mg enzyme towards vanillin1 Publication
  2. Vmax=0.063 µmol/min/mg enzyme towards trans-resveratrol1 Publication
  3. Vmax=0.022 µmol/min/mg enzyme towards curcumin1 Publication
  4. Vmax=0.013 µmol/min/mg enzyme towards etoposide1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei293UDP-glucoseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.128 399
SABIO-RKiO82381

Protein family/group databases

CAZyiGT1 Glycosyltransferase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 71C1 (EC:2.4.1.-)
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C1 (EC:2.4.1.91)
Flavonol 7-O-glucosyltransferase UGT71C1
Gene namesi
Name:UGT71C1
Ordered Locus Names:At2g29750
ORF Names:T27A16.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G29750
TAIRilocus:2060654 AT2G29750

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004090531 – 481UDP-glycosyltransferase 71C1Add BLAST481

Proteomic databases

PaxDbiO82381
PRIDEiO82381

Expressioni

Gene expression databases

ExpressionAtlasiO82381 differential
GenevisibleiO82381 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G29750.1

Structurei

3D structure databases

ProteinModelPortaliO82381
SMRiO82381
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni352 – 354UDP-glucose bindingBy similarity3
Regioni369 – 377UDP-glucose bindingBy similarity9
Regioni391 – 394UDP-glucose bindingBy similarity4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192 Eukaryota
COG1819 LUCA
HOGENOMiHOG000237568
InParanoidiO82381
OMAiITILYWG
OrthoDBiEOG093609N4
PhylomeDBiO82381

Family and domain databases

InterProiView protein in InterPro
IPR002213 UDP_glucos_trans
IPR035595 UDP_glycos_trans_CS
PfamiView protein in Pfam
PF00201 UDPGT, 1 hit
PROSITEiView protein in PROSITE
PS00375 UDPGT, 1 hit

Sequencei

Sequence statusi: Complete.

O82381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQEDAELV IIPFPFSGHI LATIELAKRL ISQDNPRIHT ITILYWGLPF
60 70 80 90 100
IPQADTIAFL RSLVKNEPRI RLVTLPEVQD PPPMELFVEF AESYILEYVK
110 120 130 140 150
KMVPIIREAL STLLSSRDES GSVRVAGLVL DFFCVPMIDV GNEFNLPSYI
160 170 180 190 200
FLTCSAGFLG MMKYLPERHR EIKSEFNRSF NEELNLIPGY VNSVPTKVLP
210 220 230 240 250
SGLFMKETYE PWVELAERFP EAKGILVNSY TALEPNGFKY FDRCPDNYPT
260 270 280 290 300
IYPIGPILCS NDRPNLDSSE RDRIITWLDD QPESSVVFLC FGSLKNLSAT
310 320 330 340 350
QINEIAQALE IVDCKFIWSF RTNPKEYASP YEALPHGFMD RVMDQGIVCG
360 370 380 390 400
WAPQVEILAH KAVGGFVSHC GWNSILESLG FGVPIATWPM YAEQQLNAFT
410 420 430 440 450
MVKELGLALE MRLDYVSEDG DIVKADEIAG TVRSLMDGVD VPKSKVKEIA
460 470 480
EAGKEAVDGG SSFLAVKRFI GDLIDGVSIS K
Length:481
Mass (Da):53,875
Last modified:November 1, 1998 - v1
Checksum:i21283D9B69C4C97C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005496 Genomic DNA Translation: AAC35226.1
CP002685 Genomic DNA Translation: AEC08300.1
BT023426 mRNA Translation: AAY56417.1
BT026458 mRNA Translation: ABH04565.1
PIRiB84700
RefSeqiNP_180536.1, NM_128529.3
UniGeneiAt.13110

Genome annotation databases

EnsemblPlantsiAT2G29750.1; AT2G29750.1; AT2G29750
GeneIDi817525
GrameneiAT2G29750.1; AT2G29750.1; AT2G29750
KEGGiath:AT2G29750

Similar proteinsi

Entry informationi

Entry nameiU71C1_ARATH
AccessioniPrimary (citable) accession number: O82381
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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