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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial

Gene

KPHMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (KPHMT1), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At2g43720), 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial (KPHMT2), 3-methyl-2-oxobutanoate hydroxymethyltransferase (AXX17_At3g55820)
  2. Putative 2-dehydropantoate 2-reductase (KPR)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83MagnesiumBy similarity1
Metal bindingi122MagnesiumBy similarity1
Binding sitei122Alpha-ketoisovalerateBy similarity1
Binding sitei152Alpha-ketoisovalerateBy similarity1
Metal bindingi154MagnesiumBy similarity1
Active sitei222Proton acceptorBy similarity1

GO - Molecular functioni

  • 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • methyltransferase activity Source: UniProtKB-KW

GO - Biological processi

  • pantothenate biosynthetic process Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G46110-MONOMER
MetaCyc:AT2G46110-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial (EC:2.1.2.11)
Alternative name(s):
Ketopantoate hydroxymethyltransferase 1
Gene namesi
Name:KPHMT1
Synonyms:PANB1
Ordered Locus Names:At2g46110
ORF Names:T3F17.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G46110
TAIRilocus:2062969 AT2G46110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48MitochondrionSequence analysisAdd BLAST48
ChainiPRO_000042956649 – 3473-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrialAdd BLAST299

Proteomic databases

PaxDbiO82357
PRIDEiO82357

Expressioni

Gene expression databases

ExpressionAtlasiO82357 baseline and differential
GenevisibleiO82357 AT

Interactioni

Protein-protein interaction databases

BioGridi4554, 1 interactor
STRINGi3702.AT2G46110.1

Structurei

3D structure databases

ProteinModelPortaliO82357
SMRiO82357
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 84Alpha-ketoisovalerate bindingBy similarity2

Sequence similaritiesi

Belongs to the PanB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2949 Eukaryota
COG0413 LUCA
HOGENOMiHOG000078427
InParanoidiO82357
KOiK00606
OMAiKYEERAG
OrthoDBiEOG09360FPX
PhylomeDBiO82357

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLTRNCS RFSKAISVRF MSNLPENTVY GGPKPQNPNQ RVTLTHLRQK
60 70 80 90 100
HRRGEPITVV TAYDYPSAVH LDTAGIDVCL VGDSASMVVH GHDTTLPISL
110 120 130 140 150
DEMLVHCRAV ARGAKRPLLV GDLPFGTYES SSSQAVDTAV RVLKEGGMDA
160 170 180 190 200
IKLEGGSASR ITAAKAIVEA GIAVIGHVGL TPQAISVLGG FRPQGRNIAS
210 220 230 240 250
AVKVVETAMA LQEAGCFSVV LECVPPPVAA AATSALKIPT IGIGAGPFCS
260 270 280 290 300
GQVLVYHDLL GMMQHPHHAK VTPKFCKQYA NVGEVINKAL MEYKEEVSKK
310 320 330 340
VFPGPSHSPY KITASELDGF LTELQKLGFD KAASAAALAA ENMEPSK
Length:347
Mass (Da):36,693
Last modified:November 1, 1998 - v1
Checksum:i1A37916DA6B97795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005397 Genomic DNA Translation: AAC62893.1
CP002685 Genomic DNA Translation: AEC10645.1
BT004820 mRNA Translation: AAO44086.1
AK227849 mRNA Translation: BAE99826.1
PIRiH84898
RefSeqiNP_182135.1, NM_130174.5
UniGeneiAt.48599
At.66444

Genome annotation databases

EnsemblPlantsiAT2G46110.1; AT2G46110.1; AT2G46110
GeneIDi819219
GrameneiAT2G46110.1; AT2G46110.1; AT2G46110
KEGGiath:AT2G46110

Similar proteinsi

Entry informationi

Entry nameiPANB1_ARATH
AccessioniPrimary (citable) accession number: O82357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health