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Protein

Aquaporin TIP4-1

Gene

TIP4-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. Transports urea in yeast cells in a pH-independent manner.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin TIP4-1
Alternative name(s):
Epsilon-tonoplast intrinsic protein
Short name:
Epsilon-TIP
Tonoplast intrinsic protein 4-1
Short name:
AtTIP4;1
Cleaved into the following chain:
Gene namesi
Name:TIP4-1
Ordered Locus Names:At2g25810
ORF Names:F17H15.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G25810.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini42 – 49VacuolarSequence analysis8
Transmembranei50 – 70Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini71 – 105CytoplasmicSequence analysisAdd BLAST35
Transmembranei106 – 126Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini127 – 137VacuolarSequence analysisAdd BLAST11
Transmembranei138 – 158Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini159 – 166CytoplasmicSequence analysis8
Transmembranei167 – 187Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini188 – 212VacuolarSequence analysisAdd BLAST25
Transmembranei213 – 233Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini234 – 249CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

  • central vacuole Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000640161 – 249Aquaporin TIP4-1Add BLAST249
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004257642 – 249Aquaporin TIP4-1, N-terminally processedAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6,N6-dimethyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiO82316.

Expressioni

Tissue specificityi

Expressed in roots.1 Publication

Developmental stagei

Starts to be expressed in seedlings from 2 days ays after germination.1 Publication

Gene expression databases

GenevisibleiO82316. AT.

Interactioni

Protein-protein interaction databases

BioGridi2475. 16 interactors.
IntActiO82316. 3 interactors.
STRINGi3702.AT2G25810.1.

Structurei

3D structure databases

ProteinModelPortaliO82316.
SMRiO82316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi79 – 81NPA 13
Motifi193 – 195NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiO82316.
KOiK09873.
OMAiGMGTPVH.
OrthoDBiEOG09360J95.
PhylomeDBiO82316.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIELGHHS EAAKPDCIKA LIVEFITTFL FVFAGVGSAM ATDSLVGNTL
60 70 80 90 100
VGLFAVAVAH AFVVAVMISA GHISGGHLNP AVTLGLLLGG HISVFRAFLY
110 120 130 140 150
WIDQLLASSA ACFLLSYLTG GMGTPVHTLA SGVSYTQGII WEIILTFSLL
160 170 180 190 200
FTVYATIVDP KKGSLDGFGP LLTGFVVGAN ILAGGAFSGA SMNPARSFGP
210 220 230 240
ALVSGNWTDH WVYWVGPLIG GGLAGFIYEN VLIDRPHVPV ADDEQPLLN
Length:249
Mass (Da):26,073
Last modified:November 1, 1998 - v1
Checksum:i1A52B34B5CEF4693
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005395 Genomic DNA. Translation: AAC42249.1.
CP002685 Genomic DNA. Translation: AEC07755.1.
BT012635 mRNA. Translation: AAT06454.1.
AK226190 mRNA. Translation: BAE98355.1.
PIRiA84653.
RefSeqiNP_180152.1. NM_128141.6.
UniGeneiAt.43822.

Genome annotation databases

EnsemblPlantsiAT2G25810.1; AT2G25810.1; AT2G25810.
GeneIDi817123.
GrameneiAT2G25810.1; AT2G25810.1; AT2G25810.
KEGGiath:AT2G25810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005395 Genomic DNA. Translation: AAC42249.1.
CP002685 Genomic DNA. Translation: AEC07755.1.
BT012635 mRNA. Translation: AAT06454.1.
AK226190 mRNA. Translation: BAE98355.1.
PIRiA84653.
RefSeqiNP_180152.1. NM_128141.6.
UniGeneiAt.43822.

3D structure databases

ProteinModelPortaliO82316.
SMRiO82316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2475. 16 interactors.
IntActiO82316. 3 interactors.
STRINGi3702.AT2G25810.1.

Proteomic databases

PaxDbiO82316.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G25810.1; AT2G25810.1; AT2G25810.
GeneIDi817123.
GrameneiAT2G25810.1; AT2G25810.1; AT2G25810.
KEGGiath:AT2G25810.

Organism-specific databases

TAIRiAT2G25810.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiO82316.
KOiK09873.
OMAiGMGTPVH.
OrthoDBiEOG09360J95.
PhylomeDBiO82316.

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiO82316.

Gene expression databases

GenevisibleiO82316. AT.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIP41_ARATH
AccessioniPrimary (citable) accession number: O82316
Secondary accession number(s): Q53XD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.