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Protein

Protein phosphatase 2C 29

Gene

PLL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi295 – 2951Manganese 1By similarity
Metal bindingi295 – 2951Manganese 2By similarity
Metal bindingi296 – 2961Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi701 – 7011Manganese 2By similarity
Metal bindingi761 – 7611Manganese 2By similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: TAIR
  • phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  • maintenance of meristem identity Source: TAIR
  • meristem structural organization Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G35350-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 29 (EC:3.1.3.16)
Short name:
AtPP2C29
Alternative name(s):
Protein POLTERGEIST-LIKE 1
Protein phosphatase 2C PLL1
Short name:
PP2C PLL1
Gene namesi
Name:PLL1
Ordered Locus Names:At2g35350
ORF Names:T32F12.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G35350.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss-of-function mutant pll1-1 (T-DNA insertion) shows suppression of clavata mutant phenotypes. Redundant with POL. Pol and pll1 double mutant inis seedling lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Protein phosphatase 2C 29PRO_0000301259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82302.
PRIDEiO82302.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, inflorescences, flowers and developing vascular tissue.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G35350.1.

Structurei

3D structure databases

ProteinModelPortaliO82302.
SMRiO82302. Positions 605-768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini260 – 770511PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi212 – 2165Poly-Lys
Compositional biasi362 – 3676Poly-Glu

Domaini

The conserved PP2C phosphatase domain (257-736) is interrupted by an insertion of approximately 200 amino acids.

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG239687.
HOGENOMiHOG000239375.
InParanoidiO82302.
OMAiPTHSDRF.
PhylomeDBiO82302.

Family and domain databases

Gene3Di3.60.40.10. 3 hits.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 2 hits.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82302-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGFSSLLP CFNQGHRNRR RHSSAANPSH SDLIDSFREP LDETLGHSYC
60 70 80 90 100
YVPSSSNRFI SPFPSDRFVS PTASFRLSPP HEPGRIRGSG SSEQLHTGFR
110 120 130 140 150
AISGASVSAN TSNSKTVLQL EDIYDDATES SFGGGVRRSV VNANGFEGTS
160 170 180 190 200
SFSALPLQPG PDRSGLFMSG PIERGATSGP LDPPAGEISR SNSAGVHFSA
210 220 230 240 250
PLGGVYSKKR RKKKKKSLSW HPIFGGEKKQ RPWVLPVSNF VVGAKKENIV
260 270 280 290 300
RPDVEAMAAS SGENDLQWAL GKAGEDRVQL AVFEKQGWLF AGIYDGFNGP
310 320 330 340 350
DAPEFLMANL YRAVHSELQG LFWELEEEDD NPTDISTREL EQQGEFEDHV
360 370 380 390 400
NEMASSSCPA TEKEEEEMGK RLTSSLEVVE VKERKRLWEL LAEAQAEDAL
410 420 430 440 450
DLSGSDRFAF SVDDAIGAGN AVSVGSKRWL LLSKLKQGLS KQGISGRKLF
460 470 480 490 500
PWKSGVEENE TEEVDNVGVE EGVDKRRKRR KAGTVDHELV LKAMSNGLEA
510 520 530 540 550
TEQAFLEMTD KVLETNPELA LMGSCLLVAL MRDDDVYIMN IGDSRALVAQ
560 570 580 590 600
YQVEETGESV ETAERVEERR NDLDRDDGNK EPLVVDSSDS TVNNEAPLPQ
610 620 630 640 650
TKLVALQLTT DHSTSIEDEV TRIKNEHPDD NHCIVNDRVK GRLKVTRAFG
660 670 680 690 700
AGFLKQPKLN DALLEMFRNE YIGTDPYISC TPSLRHYRLT ENDQFMVLSS
710 720 730 740 750
DGLYQYLSNV EVVSLAMEKF PDGDPAQHVI QELLVRAAKK AGMDFHELLD
760 770 780
IPQGDRRKYH DDCTVLVIAL GGSRIWKSSG KYL
Length:783
Mass (Da):86,523
Last modified:September 11, 2007 - v2
Checksum:i431BC94F054E5FD5
GO

Sequence cautioni

The sequence AAC36186.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAF01477.1 differs from that shown.Intron retention.Curated
The sequence BAF01477.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09098.1.
AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
PIRiE84767.
RefSeqiNP_181078.2. NM_129087.2.
UniGeneiAt.37679.
At.67838.

Genome annotation databases

EnsemblPlantsiAT2G35350.1; AT2G35350.1; AT2G35350.
GeneIDi818102.
KEGGiath:AT2G35350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09098.1.
AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
PIRiE84767.
RefSeqiNP_181078.2. NM_129087.2.
UniGeneiAt.37679.
At.67838.

3D structure databases

ProteinModelPortaliO82302.
SMRiO82302. Positions 605-768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G35350.1.

Proteomic databases

PaxDbiO82302.
PRIDEiO82302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G35350.1; AT2G35350.1; AT2G35350.
GeneIDi818102.
KEGGiath:AT2G35350.

Organism-specific databases

TAIRiAT2G35350.

Phylogenomic databases

eggNOGiNOG239687.
HOGENOMiHOG000239375.
InParanoidiO82302.
OMAiPTHSDRF.
PhylomeDBiO82302.

Enzyme and pathway databases

BioCyciARA:AT2G35350-MONOMER.

Miscellaneous databases

PROiO82302.

Family and domain databases

Gene3Di3.60.40.10. 3 hits.
InterProiIPR015655. PP2C.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 2 hits.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-783.
    Strain: cv. Columbia.
  4. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  5. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
    Song S.-K., Clark S.E.
    Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  7. "POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
    Song S.-K., Lee M.M., Clark S.E.
    Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiP2C29_ARATH
AccessioniPrimary (citable) accession number: O82302
Secondary accession number(s): Q0WN23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 24, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.