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O82302

- P2C29_ARATH

UniProt

O82302 - P2C29_ARATH

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Protein

Protein phosphatase 2C 29

Gene

PLL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi295 – 2951Manganese 1By similarity
Metal bindingi295 – 2951Manganese 2By similarity
Metal bindingi296 – 2961Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi701 – 7011Manganese 2By similarity
Metal bindingi761 – 7611Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phospholipid binding Source: TAIR
  3. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. maintenance of meristem identity Source: TAIR
  2. meristem structural organization Source: TAIR
  3. regulation of transcription, DNA-templated Source: TAIR
  4. unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G35350-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 29 (EC:3.1.3.16)
Short name:
AtPP2C29
Alternative name(s):
Protein POLTERGEIST-LIKE 1
Protein phosphatase 2C PLL1
Short name:
PP2C PLL1
Gene namesi
Name:PLL1
Ordered Locus Names:At2g35350
ORF Names:T32F12.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G35350.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss-of-function mutant pll1-1 (T-DNA insertion) shows suppression of clavata mutant phenotypes. Redundant with POL. Pol and pll1 double mutant inis seedling lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Protein phosphatase 2C 29PRO_0000301259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82302.
PRIDEiO82302.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, inflorescences, flowers and developing vascular tissue.1 Publication

Gene expression databases

GenevestigatoriO82302.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G35350.1-P.

Structurei

3D structure databases

ProteinModelPortaliO82302.
SMRiO82302. Positions 605-768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini398 – 746349PP2C-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi212 – 2165Poly-Lys
Compositional biasi362 – 3676Poly-Glu

Domaini

The conserved PP2C phosphatase domain (257-736) is interrupted by an insertion of approximately 200 amino acids.

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiNOG239687.
HOGENOMiHOG000239375.
InParanoidiO82302.
OMAiTRIKNEH.
PhylomeDBiO82302.

Family and domain databases

Gene3Di3.60.40.10. 3 hits.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 2 hits.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.

Sequencei

Sequence statusi: Complete.

O82302-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSGFSSLLP CFNQGHRNRR RHSSAANPSH SDLIDSFREP LDETLGHSYC
60 70 80 90 100
YVPSSSNRFI SPFPSDRFVS PTASFRLSPP HEPGRIRGSG SSEQLHTGFR
110 120 130 140 150
AISGASVSAN TSNSKTVLQL EDIYDDATES SFGGGVRRSV VNANGFEGTS
160 170 180 190 200
SFSALPLQPG PDRSGLFMSG PIERGATSGP LDPPAGEISR SNSAGVHFSA
210 220 230 240 250
PLGGVYSKKR RKKKKKSLSW HPIFGGEKKQ RPWVLPVSNF VVGAKKENIV
260 270 280 290 300
RPDVEAMAAS SGENDLQWAL GKAGEDRVQL AVFEKQGWLF AGIYDGFNGP
310 320 330 340 350
DAPEFLMANL YRAVHSELQG LFWELEEEDD NPTDISTREL EQQGEFEDHV
360 370 380 390 400
NEMASSSCPA TEKEEEEMGK RLTSSLEVVE VKERKRLWEL LAEAQAEDAL
410 420 430 440 450
DLSGSDRFAF SVDDAIGAGN AVSVGSKRWL LLSKLKQGLS KQGISGRKLF
460 470 480 490 500
PWKSGVEENE TEEVDNVGVE EGVDKRRKRR KAGTVDHELV LKAMSNGLEA
510 520 530 540 550
TEQAFLEMTD KVLETNPELA LMGSCLLVAL MRDDDVYIMN IGDSRALVAQ
560 570 580 590 600
YQVEETGESV ETAERVEERR NDLDRDDGNK EPLVVDSSDS TVNNEAPLPQ
610 620 630 640 650
TKLVALQLTT DHSTSIEDEV TRIKNEHPDD NHCIVNDRVK GRLKVTRAFG
660 670 680 690 700
AGFLKQPKLN DALLEMFRNE YIGTDPYISC TPSLRHYRLT ENDQFMVLSS
710 720 730 740 750
DGLYQYLSNV EVVSLAMEKF PDGDPAQHVI QELLVRAAKK AGMDFHELLD
760 770 780
IPQGDRRKYH DDCTVLVIAL GGSRIWKSSG KYL
Length:783
Mass (Da):86,523
Last modified:September 11, 2007 - v2
Checksum:i431BC94F054E5FD5
GO

Sequence cautioni

The sequence BAF01477.1 differs from that shown. Reason: Intron retention.
The sequence BAF01477.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAC36186.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09098.1.
AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
PIRiE84767.
RefSeqiNP_181078.2. NM_129087.2.
UniGeneiAt.37679.
At.67838.

Genome annotation databases

EnsemblPlantsiAT2G35350.1; AT2G35350.1; AT2G35350.
GeneIDi818102.
KEGGiath:AT2G35350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC005314 Genomic DNA. Translation: AAC36186.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC09098.1 .
AK229632 mRNA. Translation: BAF01477.1 . Sequence problems.
PIRi E84767.
RefSeqi NP_181078.2. NM_129087.2.
UniGenei At.37679.
At.67838.

3D structure databases

ProteinModelPortali O82302.
SMRi O82302. Positions 605-768.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G35350.1-P.

Proteomic databases

PaxDbi O82302.
PRIDEi O82302.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G35350.1 ; AT2G35350.1 ; AT2G35350 .
GeneIDi 818102.
KEGGi ath:AT2G35350.

Organism-specific databases

TAIRi AT2G35350.

Phylogenomic databases

eggNOGi NOG239687.
HOGENOMi HOG000239375.
InParanoidi O82302.
OMAi TRIKNEH.
PhylomeDBi O82302.

Enzyme and pathway databases

BioCyci ARA:AT2G35350-MONOMER.

Gene expression databases

Genevestigatori O82302.

Family and domain databases

Gene3Di 3.60.40.10. 3 hits.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 2 hits.
[Graphical view ]
SMARTi SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-783.
    Strain: cv. Columbia.
  4. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  5. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
    Song S.-K., Clark S.E.
    Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  7. "POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
    Song S.-K., Lee M.M., Clark S.E.
    Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiP2C29_ARATH
AccessioniPrimary (citable) accession number: O82302
Secondary accession number(s): Q0WN23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3