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O82302 (P2C29_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C 29

Short name=AtPP2C29
EC=3.1.3.16
Alternative name(s):
Protein POLTERGEIST-LIKE 1
Protein phosphatase 2C PLL1
Short name=PP2C PLL1
Gene names
Name:PLL1
Ordered Locus Names:At2g35350
ORF Names:T32F12.27
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length783 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. Ref.6 Ref.7

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in roots, leaves, stems, inflorescences, flowers and developing vascular tissue. Ref.6

Domain

The conserved PP2C phosphatase domain (257-736) is interrupted by an insertion of approximately 200 amino acids.

Disruption phenotype

Loss-of-function mutant pll1-1 (T-DNA insertion) shows suppression of clavata mutant phenotypes. Redundant with POL. Pol and pll1 double mutant inis seedling lethal. Ref.7

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence AAC36186.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAF01477.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAF01477.1 differs from that shown. Reason: Intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 783783Protein phosphatase 2C 29
PRO_0000301259

Regions

Domain398 – 746349PP2C-like
Compositional bias212 – 2165Poly-Lys
Compositional bias362 – 3676Poly-Glu

Sites

Metal binding2951Manganese 1 By similarity
Metal binding2951Manganese 2 By similarity
Metal binding2961Manganese 1; via carbonyl oxygen By similarity
Metal binding7011Manganese 2 By similarity
Metal binding7611Manganese 2 By similarity

Amino acid modifications

Modified residue1991Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
O82302 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: 431BC94F054E5FD5

FASTA78386,523
        10         20         30         40         50         60 
MGSGFSSLLP CFNQGHRNRR RHSSAANPSH SDLIDSFREP LDETLGHSYC YVPSSSNRFI 

        70         80         90        100        110        120 
SPFPSDRFVS PTASFRLSPP HEPGRIRGSG SSEQLHTGFR AISGASVSAN TSNSKTVLQL 

       130        140        150        160        170        180 
EDIYDDATES SFGGGVRRSV VNANGFEGTS SFSALPLQPG PDRSGLFMSG PIERGATSGP 

       190        200        210        220        230        240 
LDPPAGEISR SNSAGVHFSA PLGGVYSKKR RKKKKKSLSW HPIFGGEKKQ RPWVLPVSNF 

       250        260        270        280        290        300 
VVGAKKENIV RPDVEAMAAS SGENDLQWAL GKAGEDRVQL AVFEKQGWLF AGIYDGFNGP 

       310        320        330        340        350        360 
DAPEFLMANL YRAVHSELQG LFWELEEEDD NPTDISTREL EQQGEFEDHV NEMASSSCPA 

       370        380        390        400        410        420 
TEKEEEEMGK RLTSSLEVVE VKERKRLWEL LAEAQAEDAL DLSGSDRFAF SVDDAIGAGN 

       430        440        450        460        470        480 
AVSVGSKRWL LLSKLKQGLS KQGISGRKLF PWKSGVEENE TEEVDNVGVE EGVDKRRKRR 

       490        500        510        520        530        540 
KAGTVDHELV LKAMSNGLEA TEQAFLEMTD KVLETNPELA LMGSCLLVAL MRDDDVYIMN 

       550        560        570        580        590        600 
IGDSRALVAQ YQVEETGESV ETAERVEERR NDLDRDDGNK EPLVVDSSDS TVNNEAPLPQ 

       610        620        630        640        650        660 
TKLVALQLTT DHSTSIEDEV TRIKNEHPDD NHCIVNDRVK GRLKVTRAFG AGFLKQPKLN 

       670        680        690        700        710        720 
DALLEMFRNE YIGTDPYISC TPSLRHYRLT ENDQFMVLSS DGLYQYLSNV EVVSLAMEKF 

       730        740        750        760        770        780 
PDGDPAQHVI QELLVRAAKK AGMDFHELLD IPQGDRRKYH DDCTVLVIAL GGSRIWKSSG 


KYL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-783.
Strain: cv. Columbia.
[4]"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. La-0.
[5]"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
Song S.-K., Clark S.E.
Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[7]"POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
Song S.-K., Lee M.M., Clark S.E.
Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09098.1.
AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
PIRE84767.
RefSeqNP_181078.2. NM_129087.2.
UniGeneAt.37679.
At.67838.

3D structure databases

ProteinModelPortalO82302.
SMRO82302. Positions 503-774.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G35350.1-P.

Proteomic databases

PaxDbO82302.
PRIDEO82302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G35350.1; AT2G35350.1; AT2G35350.
GeneID818102.
KEGGath:AT2G35350.

Organism-specific databases

TAIRAT2G35350.

Phylogenomic databases

eggNOGNOG239687.
HOGENOMHOG000239375.
InParanoidO82302.
OMAIGDTPYI.
PhylomeDBO82302.
ProtClustDBCLSN2681196.

Enzyme and pathway databases

BioCycARA:AT2G35350-MONOMER.

Gene expression databases

GenevestigatorO82302.

Family and domain databases

Gene3D3.60.40.10. 3 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 2 hits.
[Graphical view]
SMARTSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 3 hits.
ProtoNetSearch...

Entry information

Entry nameP2C29_ARATH
AccessionPrimary (citable) accession number: O82302
Secondary accession number(s): Q0WN23
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: March 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names