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O82302

- P2C29_ARATH

UniProt

O82302 - P2C29_ARATH

Protein

Protein phosphatase 2C 29

Gene

PLL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems.2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi295 – 2951Manganese 1By similarity
    Metal bindingi295 – 2951Manganese 2By similarity
    Metal bindingi296 – 2961Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi701 – 7011Manganese 2By similarity
    Metal bindingi761 – 7611Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phospholipid binding Source: TAIR
    3. phosphoprotein phosphatase activity Source: UniProtKB-KW

    GO - Biological processi

    1. maintenance of meristem identity Source: TAIR
    2. meristem structural organization Source: TAIR
    3. regulation of transcription, DNA-templated Source: TAIR
    4. unidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G35350-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 29 (EC:3.1.3.16)
    Short name:
    AtPP2C29
    Alternative name(s):
    Protein POLTERGEIST-LIKE 1
    Protein phosphatase 2C PLL1
    Short name:
    PP2C PLL1
    Gene namesi
    Name:PLL1
    Ordered Locus Names:At2g35350
    ORF Names:T32F12.27
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G35350.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Loss-of-function mutant pll1-1 (T-DNA insertion) shows suppression of clavata mutant phenotypes. Redundant with POL. Pol and pll1 double mutant inis seedling lethal.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 783783Protein phosphatase 2C 29PRO_0000301259Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO82302.
    PRIDEiO82302.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, inflorescences, flowers and developing vascular tissue.1 Publication

    Gene expression databases

    GenevestigatoriO82302.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G35350.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO82302.
    SMRiO82302. Positions 605-768.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini398 – 746349PP2C-likeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi212 – 2165Poly-Lys
    Compositional biasi362 – 3676Poly-Glu

    Domaini

    The conserved PP2C phosphatase domain (257-736) is interrupted by an insertion of approximately 200 amino acids.

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiNOG239687.
    HOGENOMiHOG000239375.
    InParanoidiO82302.
    OMAiTRIKNEH.
    PhylomeDBiO82302.

    Family and domain databases

    Gene3Di3.60.40.10. 3 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 2 hits.
    [Graphical view]
    SMARTiSM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    O82302-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSGFSSLLP CFNQGHRNRR RHSSAANPSH SDLIDSFREP LDETLGHSYC    50
    YVPSSSNRFI SPFPSDRFVS PTASFRLSPP HEPGRIRGSG SSEQLHTGFR 100
    AISGASVSAN TSNSKTVLQL EDIYDDATES SFGGGVRRSV VNANGFEGTS 150
    SFSALPLQPG PDRSGLFMSG PIERGATSGP LDPPAGEISR SNSAGVHFSA 200
    PLGGVYSKKR RKKKKKSLSW HPIFGGEKKQ RPWVLPVSNF VVGAKKENIV 250
    RPDVEAMAAS SGENDLQWAL GKAGEDRVQL AVFEKQGWLF AGIYDGFNGP 300
    DAPEFLMANL YRAVHSELQG LFWELEEEDD NPTDISTREL EQQGEFEDHV 350
    NEMASSSCPA TEKEEEEMGK RLTSSLEVVE VKERKRLWEL LAEAQAEDAL 400
    DLSGSDRFAF SVDDAIGAGN AVSVGSKRWL LLSKLKQGLS KQGISGRKLF 450
    PWKSGVEENE TEEVDNVGVE EGVDKRRKRR KAGTVDHELV LKAMSNGLEA 500
    TEQAFLEMTD KVLETNPELA LMGSCLLVAL MRDDDVYIMN IGDSRALVAQ 550
    YQVEETGESV ETAERVEERR NDLDRDDGNK EPLVVDSSDS TVNNEAPLPQ 600
    TKLVALQLTT DHSTSIEDEV TRIKNEHPDD NHCIVNDRVK GRLKVTRAFG 650
    AGFLKQPKLN DALLEMFRNE YIGTDPYISC TPSLRHYRLT ENDQFMVLSS 700
    DGLYQYLSNV EVVSLAMEKF PDGDPAQHVI QELLVRAAKK AGMDFHELLD 750
    IPQGDRRKYH DDCTVLVIAL GGSRIWKSSG KYL 783
    Length:783
    Mass (Da):86,523
    Last modified:September 11, 2007 - v2
    Checksum:i431BC94F054E5FD5
    GO

    Sequence cautioni

    The sequence BAF01477.1 differs from that shown. Reason: Intron retention.
    The sequence BAF01477.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAC36186.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC09098.1.
    AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
    PIRiE84767.
    RefSeqiNP_181078.2. NM_129087.2.
    UniGeneiAt.37679.
    At.67838.

    Genome annotation databases

    EnsemblPlantsiAT2G35350.1; AT2G35350.1; AT2G35350.
    GeneIDi818102.
    KEGGiath:AT2G35350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005314 Genomic DNA. Translation: AAC36186.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC09098.1 .
    AK229632 mRNA. Translation: BAF01477.1 . Sequence problems.
    PIRi E84767.
    RefSeqi NP_181078.2. NM_129087.2.
    UniGenei At.37679.
    At.67838.

    3D structure databases

    ProteinModelPortali O82302.
    SMRi O82302. Positions 605-768.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G35350.1-P.

    Proteomic databases

    PaxDbi O82302.
    PRIDEi O82302.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G35350.1 ; AT2G35350.1 ; AT2G35350 .
    GeneIDi 818102.
    KEGGi ath:AT2G35350.

    Organism-specific databases

    TAIRi AT2G35350.

    Phylogenomic databases

    eggNOGi NOG239687.
    HOGENOMi HOG000239375.
    InParanoidi O82302.
    OMAi TRIKNEH.
    PhylomeDBi O82302.

    Enzyme and pathway databases

    BioCyci ARA:AT2G35350-MONOMER.

    Gene expression databases

    Genevestigatori O82302.

    Family and domain databases

    Gene3Di 3.60.40.10. 3 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 2 hits.
    [Graphical view ]
    SMARTi SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-783.
      Strain: cv. Columbia.
    4. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    5. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis."
      Song S.-K., Clark S.E.
      Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    7. "POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells."
      Song S.-K., Lee M.M., Clark S.E.
      Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    8. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiP2C29_ARATH
    AccessioniPrimary (citable) accession number: O82302
    Secondary accession number(s): Q0WN23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3