Protein phosphatase 2C 29 - Arabidopsis thaliana (Mouse-ear cress)

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O82302 (P2C29_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 29
Short name=AtPP2C29
EC=3.1.3.16

Alternative name(s):
Protein POLTERGEIST-LIKE 1
Protein phosphatase 2C PLL1
Short name=PP2C PLL1

Gene names

Name:	PLL1
Ordered Locus Names:	At2g35350
ORF Names:	T32F12.27

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of pedicel length and of CLAVATA pathways controlling stem cell identity at shoot and flower meristems. Ref.6 Ref.7
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in roots, leaves, stems, inflorescences, flowers and developing vascular tissue. Ref.6
Domain	The conserved PP2C phosphatase domain (257-736) is interrupted by an insertion of approximately 200 amino acids.
Post-translational modification	Phosphorylation level varies significantly during early response to general elicitors.
Disruption phenotype	Loss-of-function mutant pll1-1 (T-DNA insertion) shows suppression of clavata mutant phenotypes. Redundant with POL. Double mutant in POL and PLL1 is seedling lethal. Ref.7
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAC36186.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF01477.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAF01477.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Developmental protein Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maintenance of meristem identity Inferred from genetic interaction Ref.7. Source: TAIR meristem structural organization Inferred from genetic interaction Ref.6. Source: TAIR metabolic process Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from genetic interaction Ref.7. Source: TAIR unidimensional cell growth Inferred from genetic interaction Ref.6. Source: TAIR
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.4 Ref.8 PubMed 20348433. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from direct assay PubMed 20348433. Source: TAIR phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 783

783

Protein phosphatase 2C 29

PRO_0000301259

Regions

Domain

398 – 746

349

PP2C-like

Compositional bias

212 – 216

Poly-Lys

Compositional bias

362 – 367

Poly-Glu

Sites

Metal binding

295

Manganese 1 By similarity

Metal binding

295

Manganese 2 By similarity

Metal binding

296

Manganese 1; via carbonyl oxygen By similarity

Metal binding

701

Manganese 2 By similarity

Metal binding

761

Manganese 2 By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.5

Modified residue

169

Phosphoserine Ref.5 Ref.8

Modified residue

177

Phosphothreonine Ref.4

Modified residue

178

Phosphoserine Ref.4 Ref.8 Ref.9

Modified residue

189

Phosphoserine Ref.4 Ref.5

Modified residue

199

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

O82302 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: 431BC94F054E5FD5
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FASTA

783

86,523

        10         20         30         40         50         60 
MGSGFSSLLP CFNQGHRNRR RHSSAANPSH SDLIDSFREP LDETLGHSYC YVPSSSNRFI 

        70         80         90        100        110        120 
SPFPSDRFVS PTASFRLSPP HEPGRIRGSG SSEQLHTGFR AISGASVSAN TSNSKTVLQL 

       130        140        150        160        170        180 
EDIYDDATES SFGGGVRRSV VNANGFEGTS SFSALPLQPG PDRSGLFMSG PIERGATSGP 

       190        200        210        220        230        240 
LDPPAGEISR SNSAGVHFSA PLGGVYSKKR RKKKKKSLSW HPIFGGEKKQ RPWVLPVSNF 

       250        260        270        280        290        300 
VVGAKKENIV RPDVEAMAAS SGENDLQWAL GKAGEDRVQL AVFEKQGWLF AGIYDGFNGP 

       310        320        330        340        350        360 
DAPEFLMANL YRAVHSELQG LFWELEEEDD NPTDISTREL EQQGEFEDHV NEMASSSCPA 

       370        380        390        400        410        420 
TEKEEEEMGK RLTSSLEVVE VKERKRLWEL LAEAQAEDAL DLSGSDRFAF SVDDAIGAGN 

       430        440        450        460        470        480 
AVSVGSKRWL LLSKLKQGLS KQGISGRKLF PWKSGVEENE TEEVDNVGVE EGVDKRRKRR 

       490        500        510        520        530        540 
KAGTVDHELV LKAMSNGLEA TEQAFLEMTD KVLETNPELA LMGSCLLVAL MRDDDVYIMN 

       550        560        570        580        590        600 
IGDSRALVAQ YQVEETGESV ETAERVEERR NDLDRDDGNK EPLVVDSSDS TVNNEAPLPQ 

       610        620        630        640        650        660 
TKLVALQLTT DHSTSIEDEV TRIKNEHPDD NHCIVNDRVK GRLKVTRAFG AGFLKQPKLN 

       670        680        690        700        710        720 
DALLEMFRNE YIGTDPYISC TPSLRHYRLT ENDQFMVLSS DGLYQYLSNV EVVSLAMEKF 

       730        740        750        760        770        780 
PDGDPAQHVI QELLVRAAKK AGMDFHELLD IPQGDRRKYH DDCTVLVIAL GGSRIWKSSG 


KYL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-783. Strain: cv. Columbia.
[4]	"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-177; SER-178 AND SER-189, MASS SPECTROMETRY. Strain: cv. La-0.
[5]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-169; SER-189 AND SER-199, MASS SPECTROMETRY.
[6]	"POL and related phosphatases are dosage-sensitive regulators of meristem and organ development in Arabidopsis." Song S.-K., Clark S.E. Dev. Biol. 285:272-284(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[7]	"POL and PLL1 phosphatases are CLAVATA1 signaling intermediates required for Arabidopsis shoot and floral stem cells." Song S.-K., Lee M.M., Clark S.E. Development 133:4691-4698(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169 AND SER-178, MASS SPECTROMETRY. Strain: cv. Columbia.
[9]	"Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses." Nuehse T.S., Bottrill A.R., Jones A.M.E., Peck S.C. Plant J. 51:931-940(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178, MASS SPECTROMETRY.
[10]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005314 Genomic DNA. Translation: AAC36186.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC09098.1. AK229632 mRNA. Translation: BAF01477.1. Sequence problems.
IPI	IPI00547149.
PIR	E84767.
RefSeq	NP_181078.2. NM_129087.2.
UniGene	At.37679. At.67838.
3D structure databases
ProteinModelPortal	O82302.
SMR	O82302. Positions 605-768.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G35350.1-P.
Proteomic databases
PaxDb	O82302.
PRIDE	O82302.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G35350.1; AT2G35350.1; AT2G35350.
GeneID	818102.
KEGG	ath:AT2G35350.
Organism-specific databases
TAIR	At2g35350.
Phylogenomic databases
eggNOG	NOG239687.
HOGENOM	HOG000239375.
InParanoid	O82302.
OMA	FNGPEAP.
PhylomeDB	O82302.
ProtClustDB	CLSN2681196.
Gene expression databases
Genevestigator	O82302.
Family and domain databases
Gene3D	3.60.40.10. 3 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C29_ARATH

Accession

Primary (citable) accession number: O82302
Secondary accession number(s): Q0WN23

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents