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Protein

Protease Do-like 2, chloroplastic

Gene

DEGP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei159Charge relay systemBy similarity1
Active sitei190Charge relay systemBy similarity1
Active sitei268Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • photosystem II repair Source: TAIR
  • protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.279.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 2, chloroplastic (EC:3.4.21.-)
Gene namesi
Name:DEGP2
Ordered Locus Names:At2g47940
ORF Names:F17A22.33, T9J23.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47940.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast stromal thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 69Thylakoid
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000004583070 – 607Protease Do-like 2, chloroplasticAdd BLAST538

Proteomic databases

PaxDbiO82261.
PRIDEiO82261.

PTM databases

iPTMnetiO82261.

Expressioni

Inductioni

By high salt, desiccation and light stresses (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiO82261. baseline and differential.
GenevisibleiO82261. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47940.1.

Structurei

Secondary structure

1607
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi111 – 115Combined sources5
Beta strandi118 – 125Combined sources8
Beta strandi130 – 132Combined sources3
Beta strandi140 – 150Combined sources11
Beta strandi153 – 156Combined sources4
Helixi158 – 161Combined sources4
Beta strandi164 – 170Combined sources7
Beta strandi178 – 186Combined sources9
Turni187 – 190Combined sources4
Beta strandi191 – 196Combined sources6
Beta strandi199 – 202Combined sources4
Beta strandi219 – 224Combined sources6
Beta strandi227 – 229Combined sources3
Beta strandi233 – 246Combined sources14
Beta strandi253 – 261Combined sources9
Turni265 – 269Combined sources5
Beta strandi270 – 273Combined sources4
Beta strandi275 – 277Combined sources3
Beta strandi279 – 283Combined sources5
Beta strandi296 – 300Combined sources5
Helixi301 – 312Combined sources12
Turni313 – 315Combined sources3
Beta strandi325 – 329Combined sources5
Helixi333 – 339Combined sources7
Beta strandi342 – 345Combined sources4
Beta strandi347 – 352Combined sources6
Helixi357 – 360Combined sources4
Beta strandi367 – 371Combined sources5
Beta strandi377 – 383Combined sources7
Beta strandi389 – 391Combined sources3
Helixi393 – 397Combined sources5
Beta strandi404 – 411Combined sources8
Beta strandi414 – 421Combined sources8
Beta strandi446 – 450Combined sources5
Helixi453 – 456Combined sources4
Beta strandi461 – 464Combined sources4
Helixi466 – 474Combined sources9
Beta strandi485 – 490Combined sources6
Helixi494 – 496Combined sources3
Beta strandi504 – 510Combined sources7
Helixi518 – 526Combined sources9
Beta strandi530 – 537Combined sources8
Beta strandi542 – 546Combined sources5
Helixi547 – 558Combined sources12
Turni559 – 562Combined sources4
Helixi569 – 572Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FLNX-ray2.80A/B/C71-607[»]
ProteinModelPortaliO82261.
SMRiO82261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 403PDZAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 317Serine proteaseAdd BLAST200

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000239474.
InParanoidiO82261.
OMAiIGYEDMS.
OrthoDBiEOG0936059N.
PhylomeDBiO82261.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR015724. Pept_DepP2.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PANTHERiPTHR22939:SF83. PTHR22939:SF83. 1 hit.
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82261-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASVANCCF SVLNASVKIQ SSSISSPWCF VSASSLTPRA SSNIKRKSSR
60 70 80 90 100
SDSPSPILNP EKNYPGRVRD ESSNPPQKMA FKAFGSPKKE KKESLSDFSR
110 120 130 140 150
DQQTDPAKIH DASFLNAVVK VYCTHTAPDY SLPWQKQRQF TSTGSAFMIG
160 170 180 190 200
DGKLLTNAHC VEHDTQVKVK RRGDDRKYVA KVLVRGVDCD IALLSVESED
210 220 230 240 250
FWKGAEPLRL GHLPRLQDSV TVVGYPLGGD TISVTKGVVS RIEVTSYAHG
260 270 280 290 300
SSDLLGIQID AAINPGNSGG PAFNDQGECI GVAFQVYRSE ETENIGYVIP
310 320 330 340 350
TTVVSHFLTD YERNGKYTGY PCLGVLLQKL ENPALRECLK VPTNEGVLVR
360 370 380 390 400
RVEPTSDASK VLKEGDVIVS FDDLHVGCEG TVPFRSSERI AFRYLISQKF
410 420 430 440 450
AGDIAEIGII RAGEHKKVQV VLRPRVHLVP YHIDGGQPSY IIVAGLVFTP
460 470 480 490 500
LSEPLIEEEC EDTIGLKLLT KARYSVARFR GEQIVILSQV LANEVNIGYE
510 520 530 540 550
DMNNQQVLKF NGIPIRNIHH LAHLIDMCKD KYLVFEFEDN YVAVLEREAS
560 570 580 590 600
NSASLCILKD YGIPSERSAD LLEPYVDPID DTQALDQGIG DSPVSNLEIG

FDGLVWA
Length:607
Mass (Da):66,802
Last modified:June 1, 2002 - v2
Checksum:i2E1A319A4D48A9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245171 mRNA. Translation: AAK14061.1.
AC005309 Genomic DNA. Translation: AAC63648.2.
AC006072 Genomic DNA. Translation: AAM15122.1.
CP002685 Genomic DNA. Translation: AEC10914.1.
AF326865 mRNA. Translation: AAG41447.1.
AF349516 mRNA. Translation: AAK15563.1.
AY075700 mRNA. Translation: AAL77706.1.
AY102139 mRNA. Translation: AAM26706.1.
PIRiD84921.
RefSeqiNP_566115.1. NM_130361.5. [O82261-1]
UniGeneiAt.12952.

Genome annotation databases

EnsemblPlantsiAT2G47940.1; AT2G47940.1; AT2G47940. [O82261-1]
GeneIDi819406.
GrameneiAT2G47940.1; AT2G47940.1; AT2G47940.
KEGGiath:AT2G47940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245171 mRNA. Translation: AAK14061.1.
AC005309 Genomic DNA. Translation: AAC63648.2.
AC006072 Genomic DNA. Translation: AAM15122.1.
CP002685 Genomic DNA. Translation: AEC10914.1.
AF326865 mRNA. Translation: AAG41447.1.
AF349516 mRNA. Translation: AAK15563.1.
AY075700 mRNA. Translation: AAL77706.1.
AY102139 mRNA. Translation: AAM26706.1.
PIRiD84921.
RefSeqiNP_566115.1. NM_130361.5. [O82261-1]
UniGeneiAt.12952.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FLNX-ray2.80A/B/C71-607[»]
ProteinModelPortaliO82261.
SMRiO82261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G47940.1.

Protein family/group databases

MEROPSiS01.279.

PTM databases

iPTMnetiO82261.

Proteomic databases

PaxDbiO82261.
PRIDEiO82261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47940.1; AT2G47940.1; AT2G47940. [O82261-1]
GeneIDi819406.
GrameneiAT2G47940.1; AT2G47940.1; AT2G47940.
KEGGiath:AT2G47940.

Organism-specific databases

TAIRiAT2G47940.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000239474.
InParanoidiO82261.
OMAiIGYEDMS.
OrthoDBiEOG0936059N.
PhylomeDBiO82261.

Miscellaneous databases

PROiO82261.

Gene expression databases

ExpressionAtlasiO82261. baseline and differential.
GenevisibleiO82261. AT.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR015724. Pept_DepP2.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PANTHERiPTHR22939:SF83. PTHR22939:SF83. 1 hit.
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDEGP2_ARATH
AccessioniPrimary (citable) accession number: O82261
Secondary accession number(s): Q9FPE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.