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Protein

Protease Do-like 2, chloroplastic

Gene

DEGP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease that performs the primary cleavage of the photodamaged D1 protein in plant photosystem II.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei159 – 1591Charge relay systemBy similarity
Active sitei190 – 1901Charge relay systemBy similarity
Active sitei268 – 2681Charge relay systemBy similarity

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • chloroplast organization Source: TAIR
  • photosystem II repair Source: TAIR
  • protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.279.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 2, chloroplastic (EC:3.4.21.-)
Gene namesi
Name:DEGP2
Ordered Locus Names:At2g47940
ORF Names:F17A22.33, T9J23.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47940.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast stromal thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei? – 69Thylakoid
Transit peptidei1 – ?ChloroplastSequence analysis
Chaini70 – 607538Protease Do-like 2, chloroplasticPRO_0000045830Add
BLAST

Proteomic databases

PaxDbiO82261.
PRIDEiO82261.

PTM databases

iPTMnetiO82261.

Expressioni

Inductioni

By high salt, desiccation and light stresses (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiO82261. baseline and differential.
GenevisibleiO82261. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47940.1.

Structurei

Secondary structure

1
607
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi111 – 1155Combined sources
Beta strandi118 – 1258Combined sources
Beta strandi130 – 1323Combined sources
Beta strandi140 – 15011Combined sources
Beta strandi153 – 1564Combined sources
Helixi158 – 1614Combined sources
Beta strandi164 – 1707Combined sources
Beta strandi178 – 1869Combined sources
Turni187 – 1904Combined sources
Beta strandi191 – 1966Combined sources
Beta strandi199 – 2024Combined sources
Beta strandi219 – 2246Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi233 – 24614Combined sources
Beta strandi253 – 2619Combined sources
Turni265 – 2695Combined sources
Beta strandi270 – 2734Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi279 – 2835Combined sources
Beta strandi296 – 3005Combined sources
Helixi301 – 31212Combined sources
Turni313 – 3153Combined sources
Beta strandi325 – 3295Combined sources
Helixi333 – 3397Combined sources
Beta strandi342 – 3454Combined sources
Beta strandi347 – 3526Combined sources
Helixi357 – 3604Combined sources
Beta strandi367 – 3715Combined sources
Beta strandi377 – 3837Combined sources
Beta strandi389 – 3913Combined sources
Helixi393 – 3975Combined sources
Beta strandi404 – 4118Combined sources
Beta strandi414 – 4218Combined sources
Beta strandi446 – 4505Combined sources
Helixi453 – 4564Combined sources
Beta strandi461 – 4644Combined sources
Helixi466 – 4749Combined sources
Beta strandi485 – 4906Combined sources
Helixi494 – 4963Combined sources
Beta strandi504 – 5107Combined sources
Helixi518 – 5269Combined sources
Beta strandi530 – 5378Combined sources
Beta strandi542 – 5465Combined sources
Helixi547 – 55812Combined sources
Turni559 – 5624Combined sources
Helixi569 – 5724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FLNX-ray2.80A/B/C71-607[»]
ProteinModelPortaliO82261.
SMRiO82261. Positions 110-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini308 – 40396PDZAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 317200Serine proteaseAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000239474.
InParanoidiO82261.
OMAiIGYEDMS.
OrthoDBiEOG0936059N.
PhylomeDBiO82261.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR015724. Pept_DepP2.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PANTHERiPTHR22939:SF83. PTHR22939:SF83. 1 hit.
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O82261-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASVANCCF SVLNASVKIQ SSSISSPWCF VSASSLTPRA SSNIKRKSSR
60 70 80 90 100
SDSPSPILNP EKNYPGRVRD ESSNPPQKMA FKAFGSPKKE KKESLSDFSR
110 120 130 140 150
DQQTDPAKIH DASFLNAVVK VYCTHTAPDY SLPWQKQRQF TSTGSAFMIG
160 170 180 190 200
DGKLLTNAHC VEHDTQVKVK RRGDDRKYVA KVLVRGVDCD IALLSVESED
210 220 230 240 250
FWKGAEPLRL GHLPRLQDSV TVVGYPLGGD TISVTKGVVS RIEVTSYAHG
260 270 280 290 300
SSDLLGIQID AAINPGNSGG PAFNDQGECI GVAFQVYRSE ETENIGYVIP
310 320 330 340 350
TTVVSHFLTD YERNGKYTGY PCLGVLLQKL ENPALRECLK VPTNEGVLVR
360 370 380 390 400
RVEPTSDASK VLKEGDVIVS FDDLHVGCEG TVPFRSSERI AFRYLISQKF
410 420 430 440 450
AGDIAEIGII RAGEHKKVQV VLRPRVHLVP YHIDGGQPSY IIVAGLVFTP
460 470 480 490 500
LSEPLIEEEC EDTIGLKLLT KARYSVARFR GEQIVILSQV LANEVNIGYE
510 520 530 540 550
DMNNQQVLKF NGIPIRNIHH LAHLIDMCKD KYLVFEFEDN YVAVLEREAS
560 570 580 590 600
NSASLCILKD YGIPSERSAD LLEPYVDPID DTQALDQGIG DSPVSNLEIG

FDGLVWA
Length:607
Mass (Da):66,802
Last modified:June 1, 2002 - v2
Checksum:i2E1A319A4D48A9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245171 mRNA. Translation: AAK14061.1.
AC005309 Genomic DNA. Translation: AAC63648.2.
AC006072 Genomic DNA. Translation: AAM15122.1.
CP002685 Genomic DNA. Translation: AEC10914.1.
AF326865 mRNA. Translation: AAG41447.1.
AF349516 mRNA. Translation: AAK15563.1.
AY075700 mRNA. Translation: AAL77706.1.
AY102139 mRNA. Translation: AAM26706.1.
PIRiD84921.
RefSeqiNP_566115.1. NM_130361.4. [O82261-1]
UniGeneiAt.12952.

Genome annotation databases

EnsemblPlantsiAT2G47940.1; AT2G47940.1; AT2G47940. [O82261-1]
GeneIDi819406.
KEGGiath:AT2G47940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245171 mRNA. Translation: AAK14061.1.
AC005309 Genomic DNA. Translation: AAC63648.2.
AC006072 Genomic DNA. Translation: AAM15122.1.
CP002685 Genomic DNA. Translation: AEC10914.1.
AF326865 mRNA. Translation: AAG41447.1.
AF349516 mRNA. Translation: AAK15563.1.
AY075700 mRNA. Translation: AAL77706.1.
AY102139 mRNA. Translation: AAM26706.1.
PIRiD84921.
RefSeqiNP_566115.1. NM_130361.4. [O82261-1]
UniGeneiAt.12952.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FLNX-ray2.80A/B/C71-607[»]
ProteinModelPortaliO82261.
SMRiO82261. Positions 110-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G47940.1.

Protein family/group databases

MEROPSiS01.279.

PTM databases

iPTMnetiO82261.

Proteomic databases

PaxDbiO82261.
PRIDEiO82261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47940.1; AT2G47940.1; AT2G47940. [O82261-1]
GeneIDi819406.
KEGGiath:AT2G47940.

Organism-specific databases

TAIRiAT2G47940.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000239474.
InParanoidiO82261.
OMAiIGYEDMS.
OrthoDBiEOG0936059N.
PhylomeDBiO82261.

Miscellaneous databases

PROiO82261.

Gene expression databases

ExpressionAtlasiO82261. baseline and differential.
GenevisibleiO82261. AT.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR015724. Pept_DepP2.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PANTHERiPTHR22939:SF83. PTHR22939:SF83. 1 hit.
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDEGP2_ARATH
AccessioniPrimary (citable) accession number: O82261
Secondary accession number(s): Q9FPE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.