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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

ALG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man5GlcNAc2.2 Publications

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • mannosylation Source: GOC
  • protein glycosylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G47760-MONOMER.
BRENDAi2.4.1.258. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Alpha-1,3-mannosyltransferase ALG3
Asparagine-linked glycosylation protein 3
Short name:
AtALG3
Not56-like protein
Gene namesi
Name:ALG3
Ordered Locus Names:At2g47760
ORF Names:F17A22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47760.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST
Transmembranei78 – 10023HelicalSequence analysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Transmembranei155 – 17723HelicalSequence analysisAdd
BLAST
Transmembranei196 – 21621HelicalSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Transmembranei271 – 29121HelicalSequence analysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Transmembranei374 – 39421HelicalSequence analysisAdd
BLAST
Transmembranei401 – 42121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype under normal and high temperature or salt/osmotic stress conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferasePRO_0000412588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO82244.
PRIDEiO82244.

Expressioni

Gene expression databases

ExpressionAtlasiO82244. baseline and differential.
GenevisibleiO82244. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47760.1.

Structurei

3D structure databases

ProteinModelPortaliO82244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2762. Eukaryota.
ENOG410XSN8. LUCA.
HOGENOMiHOG000237555.
InParanoidiO82244.
KOiK03845.
OMAiDWKAYMD.
PhylomeDBiO82244.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82244-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI
60 70 80 90 100
IAYVPYTKID WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA
110 120 130 140 150
VQNLTGGEVY PAQILFGVLY IVNLGIVLII YVKTDVVPWW ALSLLCLSKR
160 170 180 190 200
IHSIFVLRLF NDCFAMTLLH ASMALFLYRK WHLGMLVFSG AVSVKMNVLL
210 220 230 240 250
YAPTLLLLLL KAMNIIGVVS ALAGAALVQI LVGLPFLITY PVSYIANAFD
260 270 280 290 300
LGRVFIHFWS VNFKFVPERV FVSKEFAVCL LIAHLFLLVA FANYKWCKHE
310 320 330 340 350
GGIIGFMRSR HFFLTLPSSL SFSDVSASRI ITKEHVVTAM FVGNFIGIVF
360 370 380 390 400
ARSLHYQFYS WYFYSLPYLL WRTPFPTWLR LIMFLGIELC WNVYPSTPSS
410 420 430
SGLLLCLHLI ILVGLWLAPS VDPYQLKEHP KSQIHKKA
Length:438
Mass (Da):49,243
Last modified:November 1, 1998 - v1
Checksum:iFBF2921BC5D9AC75
GO
Isoform 2 (identifier: O82244-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-136: Missing.

Show »
Length:437
Mass (Da):49,143
Checksum:i0454331960F1FDC4
GO
Isoform 3 (identifier: O82244-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-438: Missing.

Show »
Length:380
Mass (Da):42,748
Checksum:i78C5FC7C72CA0AF6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti228 – 2281V → A in AAL16193 (PubMed:14593172).Curated
Sequence conflicti228 – 2281V → A in AAP37662 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei136 – 1361Missing in isoform 2. CuratedVSP_041719
Alternative sequencei381 – 43858Missing in isoform 3. CuratedVSP_041720Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005309 Genomic DNA. Translation: AAC63631.1.
CP002685 Genomic DNA. Translation: AEC10883.1.
CP002685 Genomic DNA. Translation: AEC10884.1.
CP002685 Genomic DNA. Translation: AEC10885.1.
AF428424 mRNA. Translation: AAL16193.1.
BT008303 mRNA. Translation: AAP37662.1.
PIRiB84919.
RefSeqiNP_001031556.1. NM_001036479.1. [O82244-2]
NP_001031557.1. NM_001036480.1. [O82244-3]
NP_182297.1. NM_130343.3. [O82244-1]
UniGeneiAt.12941.

Genome annotation databases

EnsemblPlantsiAT2G47760.1; AT2G47760.1; AT2G47760. [O82244-1]
GeneIDi819388.
KEGGiath:AT2G47760.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005309 Genomic DNA. Translation: AAC63631.1.
CP002685 Genomic DNA. Translation: AEC10883.1.
CP002685 Genomic DNA. Translation: AEC10884.1.
CP002685 Genomic DNA. Translation: AEC10885.1.
AF428424 mRNA. Translation: AAL16193.1.
BT008303 mRNA. Translation: AAP37662.1.
PIRiB84919.
RefSeqiNP_001031556.1. NM_001036479.1. [O82244-2]
NP_001031557.1. NM_001036480.1. [O82244-3]
NP_182297.1. NM_130343.3. [O82244-1]
UniGeneiAt.12941.

3D structure databases

ProteinModelPortaliO82244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G47760.1.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Proteomic databases

PaxDbiO82244.
PRIDEiO82244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47760.1; AT2G47760.1; AT2G47760. [O82244-1]
GeneIDi819388.
KEGGiath:AT2G47760.

Organism-specific databases

TAIRiAT2G47760.

Phylogenomic databases

eggNOGiKOG2762. Eukaryota.
ENOG410XSN8. LUCA.
HOGENOMiHOG000237555.
InParanoidiO82244.
KOiK03845.
OMAiDWKAYMD.
PhylomeDBiO82244.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT2G47760-MONOMER.
BRENDAi2.4.1.258. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiO82244.

Gene expression databases

ExpressionAtlasiO82244. baseline and differential.
GenevisibleiO82244. AT.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Identification of the gene encoding the alpha1,3-mannosyltransferase (ALG3) in Arabidopsis and characterization of downstream n-glycan processing."
    Henquet M., Lehle L., Schreuder M., Rouwendal G., Molthoff J., Helsper J., van der Krol S., Bosch D.
    Plant Cell 20:1652-1664(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  5. "Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans."
    Kajiura H., Seki T., Fujiyama K.
    Glycobiology 20:736-751(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiALG3_ARATH
AccessioniPrimary (citable) accession number: O82244
Secondary accession number(s): F4IM37, F4IM39, Q944H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.