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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

ALG3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man5GlcNAc2.2 Publications

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein glycosylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G47760-MONOMER.
BRENDAi2.4.1.258. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Alpha-1,3-mannosyltransferase ALG3
Asparagine-linked glycosylation protein 3
Short name:
AtALG3
Not56-like protein
Gene namesi
Name:ALG3
Ordered Locus Names:At2g47760
ORF Names:F17A22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47760.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Transmembranei78 – 100HelicalSequence analysisAdd BLAST23
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Transmembranei155 – 177HelicalSequence analysisAdd BLAST23
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Transmembranei271 – 291HelicalSequence analysisAdd BLAST21
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Transmembranei401 – 421HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype under normal and high temperature or salt/osmotic stress conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004125881 – 438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO82244.

Expressioni

Gene expression databases

ExpressionAtlasiO82244. baseline and differential.
GenevisibleiO82244. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47760.1.

Structurei

3D structure databases

ProteinModelPortaliO82244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2762. Eukaryota.
ENOG410XSN8. LUCA.
HOGENOMiHOG000237555.
InParanoidiO82244.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG09360EUK.
PhylomeDBiO82244.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82244-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI
60 70 80 90 100
IAYVPYTKID WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA
110 120 130 140 150
VQNLTGGEVY PAQILFGVLY IVNLGIVLII YVKTDVVPWW ALSLLCLSKR
160 170 180 190 200
IHSIFVLRLF NDCFAMTLLH ASMALFLYRK WHLGMLVFSG AVSVKMNVLL
210 220 230 240 250
YAPTLLLLLL KAMNIIGVVS ALAGAALVQI LVGLPFLITY PVSYIANAFD
260 270 280 290 300
LGRVFIHFWS VNFKFVPERV FVSKEFAVCL LIAHLFLLVA FANYKWCKHE
310 320 330 340 350
GGIIGFMRSR HFFLTLPSSL SFSDVSASRI ITKEHVVTAM FVGNFIGIVF
360 370 380 390 400
ARSLHYQFYS WYFYSLPYLL WRTPFPTWLR LIMFLGIELC WNVYPSTPSS
410 420 430
SGLLLCLHLI ILVGLWLAPS VDPYQLKEHP KSQIHKKA
Length:438
Mass (Da):49,243
Last modified:November 1, 1998 - v1
Checksum:iFBF2921BC5D9AC75
GO
Isoform 2 (identifier: O82244-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-136: Missing.

Show »
Length:437
Mass (Da):49,143
Checksum:i0454331960F1FDC4
GO
Isoform 3 (identifier: O82244-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     381-438: Missing.

Show »
Length:380
Mass (Da):42,748
Checksum:i78C5FC7C72CA0AF6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228V → A in AAL16193 (PubMed:14593172).Curated1
Sequence conflicti228V → A in AAP37662 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041719136Missing in isoform 2. Curated1
Alternative sequenceiVSP_041720381 – 438Missing in isoform 3. CuratedAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005309 Genomic DNA. Translation: AAC63631.1.
CP002685 Genomic DNA. Translation: AEC10883.1.
CP002685 Genomic DNA. Translation: AEC10884.1.
CP002685 Genomic DNA. Translation: AEC10885.1.
AF428424 mRNA. Translation: AAL16193.1.
BT008303 mRNA. Translation: AAP37662.1.
PIRiB84919.
RefSeqiNP_001031556.1. NM_001036479.1. [O82244-2]
NP_001031557.1. NM_001036480.1. [O82244-3]
NP_182297.1. NM_130343.4. [O82244-1]
UniGeneiAt.12941.

Genome annotation databases

EnsemblPlantsiAT2G47760.1; AT2G47760.1; AT2G47760. [O82244-1]
GeneIDi819388.
GrameneiAT2G47760.1; AT2G47760.1; AT2G47760.
KEGGiath:AT2G47760.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005309 Genomic DNA. Translation: AAC63631.1.
CP002685 Genomic DNA. Translation: AEC10883.1.
CP002685 Genomic DNA. Translation: AEC10884.1.
CP002685 Genomic DNA. Translation: AEC10885.1.
AF428424 mRNA. Translation: AAL16193.1.
BT008303 mRNA. Translation: AAP37662.1.
PIRiB84919.
RefSeqiNP_001031556.1. NM_001036479.1. [O82244-2]
NP_001031557.1. NM_001036480.1. [O82244-3]
NP_182297.1. NM_130343.4. [O82244-1]
UniGeneiAt.12941.

3D structure databases

ProteinModelPortaliO82244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G47760.1.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Proteomic databases

PaxDbiO82244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47760.1; AT2G47760.1; AT2G47760. [O82244-1]
GeneIDi819388.
GrameneiAT2G47760.1; AT2G47760.1; AT2G47760.
KEGGiath:AT2G47760.

Organism-specific databases

TAIRiAT2G47760.

Phylogenomic databases

eggNOGiKOG2762. Eukaryota.
ENOG410XSN8. LUCA.
HOGENOMiHOG000237555.
InParanoidiO82244.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG09360EUK.
PhylomeDBiO82244.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:AT2G47760-MONOMER.
BRENDAi2.4.1.258. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiO82244.

Gene expression databases

ExpressionAtlasiO82244. baseline and differential.
GenevisibleiO82244. AT.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG3_ARATH
AccessioniPrimary (citable) accession number: O82244
Secondary accession number(s): F4IM37, F4IM39, Q944H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.