Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

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O82244 (ALG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Alpha-1,3-mannosyltransferase ALG3
Asparagine-linked glycosylation protein 3
Short name=AtALG3
Not56-like protein

Gene names

Name:	ALG3
Ordered Locus Names:	At2g47760
ORF Names:	F17A22.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man₅GlcNAc₂. Ref.4 Ref.5
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. Ref.4
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4 Ref.5.
Disruption phenotype	No obvious phenotype under normal and high temperature or salt/osmotic stress conditions. Ref.5
Miscellaneous	In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.
Sequence similarities	Belongs to the glycosyltransferase 58 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from mutant phenotype Ref.4. Source: TAIR
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.4. Source: TAIR endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,3-mannosyltransferase activity Inferred from direct assay Ref.4. Source: TAIR dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O82244-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O82244-2) The sequence of this isoform differs from the canonical sequence as follows: 136-136: Missing.

Isoform 3 (identifier: O82244-3) The sequence of this isoform differs from the canonical sequence as follows: 381-438: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 438

438

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

PRO_0000412588

Regions

Transmembrane

32 – 52

Helical; Potential

Transmembrane

78 – 100

Helical; Potential

Transmembrane

112 – 132

Helical; Potential

Transmembrane

155 – 177

Helical; Potential

Transmembrane

196 – 216

Helical; Potential

Transmembrane

218 – 238

Helical; Potential

Transmembrane

271 – 291

Helical; Potential

Transmembrane

330 – 350

Helical; Potential

Transmembrane

374 – 394

Helical; Potential

Transmembrane

401 – 421

Helical; Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

103

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

136

Missing in isoform 2.

VSP_041719

Alternative sequence

381 – 438

Missing in isoform 3.

VSP_041720

Experimental info

Sequence conflict

228

V → A in AAL16193. Ref.3

Sequence conflict

228

V → A in AAP37662. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FBF2921BC5D9AC75 Show »	FASTA	438	49,243
10 20 30 40 50 60 MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI IAYVPYTKID 70 80 90 100 110 120 WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA VQNLTGGEVY PAQILFGVLY 130 140 150 160 170 180 IVNLGIVLII YVKTDVVPWW ALSLLCLSKR IHSIFVLRLF NDCFAMTLLH ASMALFLYRK 190 200 210 220 230 240 WHLGMLVFSG AVSVKMNVLL YAPTLLLLLL KAMNIIGVVS ALAGAALVQI LVGLPFLITY 250 260 270 280 290 300 PVSYIANAFD LGRVFIHFWS VNFKFVPERV FVSKEFAVCL LIAHLFLLVA FANYKWCKHE 310 320 330 340 350 360 GGIIGFMRSR HFFLTLPSSL SFSDVSASRI ITKEHVVTAM FVGNFIGIVF ARSLHYQFYS 370 380 390 400 410 420 WYFYSLPYLL WRTPFPTWLR LIMFLGIELC WNVYPSTPSS SGLLLCLHLI ILVGLWLAPS 430 VDPYQLKEHP KSQIHKKA « Hide
Isoform 2 [UniParc]. Checksum: 0454331960F1FDC4 Show »	FASTA	437	49,143
10 20 30 40 50 60 MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI IAYVPYTKID 70 80 90 100 110 120 WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA VQNLTGGEVY PAQILFGVLY 130 140 150 160 170 180 IVNLGIVLII YVKTDVPWWA LSLLCLSKRI HSIFVLRLFN DCFAMTLLHA SMALFLYRKW 190 200 210 220 230 240 HLGMLVFSGA VSVKMNVLLY APTLLLLLLK AMNIIGVVSA LAGAALVQIL VGLPFLITYP 250 260 270 280 290 300 VSYIANAFDL GRVFIHFWSV NFKFVPERVF VSKEFAVCLL IAHLFLLVAF ANYKWCKHEG 310 320 330 340 350 360 GIIGFMRSRH FFLTLPSSLS FSDVSASRII TKEHVVTAMF VGNFIGIVFA RSLHYQFYSW 370 380 390 400 410 420 YFYSLPYLLW RTPFPTWLRL IMFLGIELCW NVYPSTPSSS GLLLCLHLII LVGLWLAPSV 430 DPYQLKEHPK SQIHKKA « Hide
Isoform 3 [UniParc]. Checksum: 78C5FC7C72CA0AF6 Show »	FASTA	380	42,748
10 20 30 40 50 60 MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI IAYVPYTKID 70 80 90 100 110 120 WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA VQNLTGGEVY PAQILFGVLY 130 140 150 160 170 180 IVNLGIVLII YVKTDVVPWW ALSLLCLSKR IHSIFVLRLF NDCFAMTLLH ASMALFLYRK 190 200 210 220 230 240 WHLGMLVFSG AVSVKMNVLL YAPTLLLLLL KAMNIIGVVS ALAGAALVQI LVGLPFLITY 250 260 270 280 290 300 PVSYIANAFD LGRVFIHFWS VNFKFVPERV FVSKEFAVCL LIAHLFLLVA FANYKWCKHE 310 320 330 340 350 360 GGIIGFMRSR HFFLTLPSSL SFSDVSASRI ITKEHVVTAM FVGNFIGIVF ARSLHYQFYS 370 380 WYFYSLPYLL WRTPFPTWLR « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Identification of the gene encoding the alpha1,3-mannosyltransferase (ALG3) in Arabidopsis and characterization of downstream n-glycan processing." Henquet M., Lehle L., Schreuder M., Rouwendal G., Molthoff J., Helsper J., van der Krol S., Bosch D. Plant Cell 20:1652-1664(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[5]	"Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans." Kajiura H., Seki T., Fujiyama K. Glycobiology 20:736-751(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005309 Genomic DNA. Translation: AAC63631.1. CP002685 Genomic DNA. Translation: AEC10883.1. CP002685 Genomic DNA. Translation: AEC10884.1. CP002685 Genomic DNA. Translation: AEC10885.1. AF428424 mRNA. Translation: AAL16193.1. BT008303 mRNA. Translation: AAP37662.1.
IPI	IPI00531689. IPI00656934. IPI00657330.
PIR	B84919.
RefSeq	NP_001031556.1. NM_001036479.1. NP_001031557.1. NM_001036480.1. NP_182297.1. NM_130343.3.
UniGene	At.12941.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT58. Glycosyltransferase Family 58.
Proteomic databases
PaxDb	O82244.
PRIDE	O82244.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G47760.1; AT2G47760.1; AT2G47760.
GeneID	819388.
KEGG	ath:AT2G47760.
Organism-specific databases
TAIR	At2g47760.
Phylogenomic databases
eggNOG	NOG241920.
HOGENOM	HOG000237555.
InParanoid	O82244.
KO	K03845.
OMA	LAVSVKM.
PhylomeDB	O82244.
ProtClustDB	CLSN2683724.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	O82244.
Genevestigator	O82244.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. PTHR12646. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_ARATH

Accession

Primary (citable) accession number: O82244
Secondary accession number(s): F4IM37, F4IM39, Q944H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents