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O82244 (ALG3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

EC=2.4.1.258
Alternative name(s):
Alpha-1,3-mannosyltransferase ALG3
Asparagine-linked glycosylation protein 3
Short name=AtALG3
Not56-like protein
Gene names
Name:ALG3
Ordered Locus Names:At2g47760
ORF Names:F17A22.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for N-linked oligosaccharide assembly. Adds the sixth mannose residue in an alpha-1,3 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man5GlcNAc2. Ref.4 Ref.5

Catalytic activity

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. Ref.4

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4 Ref.5.

Disruption phenotype

No obvious phenotype under normal and high temperature or salt/osmotic stress conditions. Ref.5

Miscellaneous

In the absence of ALG3 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.

Sequence similarities

Belongs to the glycosyltransferase 58 family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O82244-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O82244-2)

The sequence of this isoform differs from the canonical sequence as follows:
     136-136: Missing.
Isoform 3 (identifier: O82244-3)

The sequence of this isoform differs from the canonical sequence as follows:
     381-438: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
PRO_0000412588

Regions

Transmembrane32 – 5221Helical; Potential
Transmembrane78 – 10023Helical; Potential
Transmembrane112 – 13221Helical; Potential
Transmembrane155 – 17723Helical; Potential
Transmembrane196 – 21621Helical; Potential
Transmembrane218 – 23821Helical; Potential
Transmembrane271 – 29121Helical; Potential
Transmembrane330 – 35021Helical; Potential
Transmembrane374 – 39421Helical; Potential
Transmembrane401 – 42121Helical; Potential

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1361Missing in isoform 2.
VSP_041719
Alternative sequence381 – 43858Missing in isoform 3.
VSP_041720

Experimental info

Sequence conflict2281V → A in AAL16193. Ref.3
Sequence conflict2281V → A in AAP37662. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: FBF2921BC5D9AC75

FASTA43849,243
        10         20         30         40         50         60 
MAGASSPASL RASRSRRLGK ETNRSDLFKK PAVPFAFALI LADAILVALI IAYVPYTKID 

        70         80         90        100        110        120 
WDAYMSQVSG FLGGERDYGN LKGDTGPLVY PAGFLYVYSA VQNLTGGEVY PAQILFGVLY 

       130        140        150        160        170        180 
IVNLGIVLII YVKTDVVPWW ALSLLCLSKR IHSIFVLRLF NDCFAMTLLH ASMALFLYRK 

       190        200        210        220        230        240 
WHLGMLVFSG AVSVKMNVLL YAPTLLLLLL KAMNIIGVVS ALAGAALVQI LVGLPFLITY 

       250        260        270        280        290        300 
PVSYIANAFD LGRVFIHFWS VNFKFVPERV FVSKEFAVCL LIAHLFLLVA FANYKWCKHE 

       310        320        330        340        350        360 
GGIIGFMRSR HFFLTLPSSL SFSDVSASRI ITKEHVVTAM FVGNFIGIVF ARSLHYQFYS 

       370        380        390        400        410        420 
WYFYSLPYLL WRTPFPTWLR LIMFLGIELC WNVYPSTPSS SGLLLCLHLI ILVGLWLAPS 

       430 
VDPYQLKEHP KSQIHKKA 

« Hide

Isoform 2 [UniParc].

Checksum: 0454331960F1FDC4
Show »

FASTA43749,143
Isoform 3 [UniParc].

Checksum: 78C5FC7C72CA0AF6
Show »

FASTA38042,748

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Identification of the gene encoding the alpha1,3-mannosyltransferase (ALG3) in Arabidopsis and characterization of downstream n-glycan processing."
Henquet M., Lehle L., Schreuder M., Rouwendal G., Molthoff J., Helsper J., van der Krol S., Bosch D.
Plant Cell 20:1652-1664(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[5]"Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans."
Kajiura H., Seki T., Fujiyama K.
Glycobiology 20:736-751(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC005309 Genomic DNA. Translation: AAC63631.1.
CP002685 Genomic DNA. Translation: AEC10883.1.
CP002685 Genomic DNA. Translation: AEC10884.1.
CP002685 Genomic DNA. Translation: AEC10885.1.
AF428424 mRNA. Translation: AAL16193.1.
BT008303 mRNA. Translation: AAP37662.1.
IPIIPI00531689.
IPI00656934.
IPI00657330.
PIRB84919.
RefSeqNP_001031556.1. NM_001036479.1.
NP_001031557.1. NM_001036480.1.
NP_182297.1. NM_130343.3.
UniGeneAt.12941.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT58. Glycosyltransferase Family 58.

Proteomic databases

PaxDbO82244.
PRIDEO82244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G47760.1; AT2G47760.1; AT2G47760.
GeneID819388.
KEGGath:AT2G47760.

Organism-specific databases

TAIRAt2g47760.

Phylogenomic databases

eggNOGNOG241920.
HOGENOMHOG000237555.
InParanoidO82244.
KOK03845.
OMALAVSVKM.
PhylomeDBO82244.
ProtClustDBCLSN2683724.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressO82244.
GenevestigatorO82244.

Family and domain databases

InterProIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERPTHR12646. PTHR12646. 1 hit.
PfamPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALG3_ARATH
AccessionPrimary (citable) accession number: O82244
Secondary accession number(s): F4IM37, F4IM39, Q944H6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: November 1, 1998
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families