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Protein

Eukaryotic translation initiation factor isoform 4G-2

Gene

EIF(ISO)4G2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the accumulation of some potyvirus during viral infection.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor isoform 4G-2
Short name:
eIF(iso)4G-2
Gene namesi
Name:EIF(ISO)4G2
Ordered Locus Names:At2g24050
ORF Names:T29E15.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G24050.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Displays no resistance to potyvirus. Possesses reduced amounts of chlorophyll a and b. I4g1 and i4g2 double mutants show reduced germination rates, slow growth rates, moderate chlorosis, late flowering, impaired fertility and reduced long term seed viability. They also exhibit altered responses to dehydration, salinity, and heat stress. The i4g1 and i4g2 double mutant has reduced amounts of chlorophyll a and b.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004205431 – 747Eukaryotic translation initiation factor isoform 4G-2Add BLAST747

Proteomic databases

PaxDbiO82233.

PTM databases

iPTMnetiO82233.

Expressioni

Gene expression databases

GenevisibleiO82233. AT.

Interactioni

Subunit structurei

EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.1 Publication

Protein-protein interaction databases

BioGridi2291. 3 interactors.
IntActiO82233. 3 interactors.
STRINGi3702.AT2G24050.1.

Structurei

3D structure databases

ProteinModelPortaliO82233.
SMRiO82233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini176 – 400MIF4GPROSITE-ProRule annotationAdd BLAST225
Domaini582 – 704MIPROSITE-ProRule annotationAdd BLAST123

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 19Poly-Gly5
Compositional biasi29 – 33Poly-Ser5
Compositional biasi436 – 440Poly-Asn5

Sequence similaritiesi

Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated

Phylogenomic databases

eggNOGiENOG410IPI8. Eukaryota.
ENOG4111HUF. LUCA.
HOGENOMiHOG000239254.
InParanoidiO82233.
KOiK03260.
OMAiMEDEWFR.
OrthoDBiEOG0936087I.
PhylomeDBiO82233.

Family and domain databases

Gene3Di1.25.40.180. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQQGEPSVL SLRPGGGGGK SRLFVPRFSS SSSFDLTNGG SEETPFPVKR
60 70 80 90 100
ENSGERVRFS REEILQHRES VQVSDEILRR CKEIAVELFG EEQSWGNHAA
110 120 130 140 150
ESKITNHTQH RHTETDNRDW HSRSQIPTSG KEWLRDDPRE AKSTWQGSGP
160 170 180 190 200
TPVLIKAEVP WSAKRGALSD KDRVVKSVKG ILNKLTPEKY ELLKGQLIDA
210 220 230 240 250
GITSADILKE VIQLIFENAI LQPTFCEMYA LLCFDINGQL PSFPSEEPGG
260 270 280 290 300
KEITFKRVLL NNCQEAFEGA GKLKEEIRQM TNPDQEMERM DKEKMAKLRT
310 320 330 340 350
LGNIRLIGEL LKQKMVPEKI VHHIVQELLG DDTKACPAEG DVEALCQFFI
360 370 380 390 400
TIGKQLDDSP RSRGINDTYF GRLKELARHP QLELRLRFMV QNVVDLRANK
410 420 430 440 450
WVPRREEVKA KKINEIHSEA ERNLGMRPGA MASMRNNNNN RAAVSGAADG
460 470 480 490 500
MGLGNILGRP GTGGMMPGMP GTRVMPMDED GWEMARTRSM PRGNRQTVQQ
510 520 530 540 550
PRFQPPPAIN KSLSVNSRLL PQGSGGLLNG GGRPSPLLQG NGSSSAPQAS
560 570 580 590 600
KPIPTVEKPQ PRSQPQPQPQ AAPLANSLNA GELERKTKSL LEEYFSIRLV
610 620 630 640 650
DEALQCVEEL KSPSYHPELV KETISLGLEK NPPLVEPIAK LLKHLISKNV
660 670 680 690 700
LTSKDLGAGC LLYGSMLDDI GIDLPKAPNS FGEFLGELVS AKVLDFELVR
710 720 730 740
DVLKKMEDEW FRKTVLNAVI KSVRECPSGQ SVLDSQAVEV EACQSLL
Length:747
Mass (Da):82,890
Last modified:November 1, 1998 - v1
Checksum:iC7AFC0731A8341FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti576N → D in AAL32572 (PubMed:14593172).Curated1
Sequence conflicti576N → D in AAN72098 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005170 Genomic DNA. Translation: AAC63673.1.
CP002685 Genomic DNA. Translation: AEC07523.1.
AY062494 mRNA. Translation: AAL32572.1.
BT002087 mRNA. Translation: AAN72098.1.
PIRiA84632.
RefSeqiNP_179983.1. NM_127967.4.
UniGeneiAt.12924.

Genome annotation databases

EnsemblPlantsiAT2G24050.1; AT2G24050.1; AT2G24050.
GeneIDi816939.
GrameneiAT2G24050.1; AT2G24050.1; AT2G24050.
KEGGiath:AT2G24050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005170 Genomic DNA. Translation: AAC63673.1.
CP002685 Genomic DNA. Translation: AEC07523.1.
AY062494 mRNA. Translation: AAL32572.1.
BT002087 mRNA. Translation: AAN72098.1.
PIRiA84632.
RefSeqiNP_179983.1. NM_127967.4.
UniGeneiAt.12924.

3D structure databases

ProteinModelPortaliO82233.
SMRiO82233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2291. 3 interactors.
IntActiO82233. 3 interactors.
STRINGi3702.AT2G24050.1.

PTM databases

iPTMnetiO82233.

Proteomic databases

PaxDbiO82233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G24050.1; AT2G24050.1; AT2G24050.
GeneIDi816939.
GrameneiAT2G24050.1; AT2G24050.1; AT2G24050.
KEGGiath:AT2G24050.

Organism-specific databases

TAIRiAT2G24050.

Phylogenomic databases

eggNOGiENOG410IPI8. Eukaryota.
ENOG4111HUF. LUCA.
HOGENOMiHOG000239254.
InParanoidiO82233.
KOiK03260.
OMAiMEDEWFR.
OrthoDBiEOG0936087I.
PhylomeDBiO82233.

Miscellaneous databases

PROiO82233.

Gene expression databases

GenevisibleiO82233. AT.

Family and domain databases

Gene3Di1.25.40.180. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4G2_ARATH
AccessioniPrimary (citable) accession number: O82233
Secondary accession number(s): Q8W4L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.