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Protein

Eukaryotic translation initiation factor isoform 4G-2

Gene

EIF(ISO)4G2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the accumulation of some potyvirus during viral infection.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-ATH-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-ATH-166208 mTORC1-mediated signalling
R-ATH-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-ATH-72702 Ribosomal scanning and start codon recognition
R-ATH-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-ATH-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor isoform 4G-2
Short name:
eIF(iso)4G-2
Gene namesi
Name:EIF(ISO)4G2
Ordered Locus Names:At2g24050
ORF Names:T29E15.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G24050
TAIRilocus:2061370 AT2G24050

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Displays no resistance to potyvirus. Possesses reduced amounts of chlorophyll a and b. I4g1 and i4g2 double mutants show reduced germination rates, slow growth rates, moderate chlorosis, late flowering, impaired fertility and reduced long term seed viability. They also exhibit altered responses to dehydration, salinity, and heat stress. The i4g1 and i4g2 double mutant has reduced amounts of chlorophyll a and b.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004205431 – 747Eukaryotic translation initiation factor isoform 4G-2Add BLAST747

Proteomic databases

PaxDbiO82233

PTM databases

iPTMnetiO82233

Expressioni

Gene expression databases

ExpressionAtlasiO82233 baseline and differential
GenevisibleiO82233 AT

Interactioni

Subunit structurei

EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.1 Publication

Protein-protein interaction databases

BioGridi2291, 3 interactors
IntActiO82233, 3 interactors
STRINGi3702.AT2G24050.1

Structurei

3D structure databases

ProteinModelPortaliO82233
SMRiO82233
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini176 – 400MIF4GPROSITE-ProRule annotationAdd BLAST225
Domaini582 – 704MIPROSITE-ProRule annotationAdd BLAST123

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 19Poly-Gly5
Compositional biasi29 – 33Poly-Ser5
Compositional biasi436 – 440Poly-Asn5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IPI8 Eukaryota
ENOG4111HUF LUCA
HOGENOMiHOG000239254
InParanoidiO82233
KOiK03260
OMAiMEKDHQP
OrthoDBiEOG0936087I
PhylomeDBiO82233

Family and domain databases

Gene3Di1.25.40.180, 2 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
SMARTiView protein in SMART
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit

Sequencei

Sequence statusi: Complete.

O82233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQQGEPSVL SLRPGGGGGK SRLFVPRFSS SSSFDLTNGG SEETPFPVKR
60 70 80 90 100
ENSGERVRFS REEILQHRES VQVSDEILRR CKEIAVELFG EEQSWGNHAA
110 120 130 140 150
ESKITNHTQH RHTETDNRDW HSRSQIPTSG KEWLRDDPRE AKSTWQGSGP
160 170 180 190 200
TPVLIKAEVP WSAKRGALSD KDRVVKSVKG ILNKLTPEKY ELLKGQLIDA
210 220 230 240 250
GITSADILKE VIQLIFENAI LQPTFCEMYA LLCFDINGQL PSFPSEEPGG
260 270 280 290 300
KEITFKRVLL NNCQEAFEGA GKLKEEIRQM TNPDQEMERM DKEKMAKLRT
310 320 330 340 350
LGNIRLIGEL LKQKMVPEKI VHHIVQELLG DDTKACPAEG DVEALCQFFI
360 370 380 390 400
TIGKQLDDSP RSRGINDTYF GRLKELARHP QLELRLRFMV QNVVDLRANK
410 420 430 440 450
WVPRREEVKA KKINEIHSEA ERNLGMRPGA MASMRNNNNN RAAVSGAADG
460 470 480 490 500
MGLGNILGRP GTGGMMPGMP GTRVMPMDED GWEMARTRSM PRGNRQTVQQ
510 520 530 540 550
PRFQPPPAIN KSLSVNSRLL PQGSGGLLNG GGRPSPLLQG NGSSSAPQAS
560 570 580 590 600
KPIPTVEKPQ PRSQPQPQPQ AAPLANSLNA GELERKTKSL LEEYFSIRLV
610 620 630 640 650
DEALQCVEEL KSPSYHPELV KETISLGLEK NPPLVEPIAK LLKHLISKNV
660 670 680 690 700
LTSKDLGAGC LLYGSMLDDI GIDLPKAPNS FGEFLGELVS AKVLDFELVR
710 720 730 740
DVLKKMEDEW FRKTVLNAVI KSVRECPSGQ SVLDSQAVEV EACQSLL
Length:747
Mass (Da):82,890
Last modified:November 1, 1998 - v1
Checksum:iC7AFC0731A8341FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti576N → D in AAL32572 (PubMed:14593172).Curated1
Sequence conflicti576N → D in AAN72098 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005170 Genomic DNA Translation: AAC63673.1
CP002685 Genomic DNA Translation: AEC07523.1
AY062494 mRNA Translation: AAL32572.1
BT002087 mRNA Translation: AAN72098.1
PIRiA84632
RefSeqiNP_179983.1, NM_127967.4
UniGeneiAt.12924

Genome annotation databases

EnsemblPlantsiAT2G24050.1; AT2G24050.1; AT2G24050
GeneIDi816939
GrameneiAT2G24050.1; AT2G24050.1; AT2G24050
KEGGiath:AT2G24050

Similar proteinsi

Entry informationi

Entry nameiIF4G2_ARATH
AccessioniPrimary (citable) accession number: O82233
Secondary accession number(s): Q8W4L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health