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O82200

- MIOX2_ARATH

UniProt

O82200 - MIOX2_ARATH

Protein

Inositol oxygenase 2

Gene

MIOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

    Catalytic activityi

    Myo-inositol + O2 = D-glucuronate + H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571SubstrateBy similarity
    Metal bindingi128 – 1281Iron 1By similarity
    Metal bindingi153 – 1531Iron 1By similarity
    Metal bindingi154 – 1541Iron 1By similarity
    Metal bindingi154 – 1541Iron 2By similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Metal bindingi226 – 2261Iron 2By similarity
    Metal bindingi252 – 2521Iron 2By similarity
    Metal bindingi285 – 2851Iron 1By similarity

    GO - Molecular functioni

    1. inositol oxygenase activity Source: TAIR
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. inositol catabolic process Source: InterPro
    2. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
    3. syncytium formation Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00111; UER00527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol oxygenase 2 (EC:1.13.99.1)
    Alternative name(s):
    Myo-inositol oxygenase 2
    Short name:
    AtMIOX2
    Short name:
    MI oxygenase 2
    Gene namesi
    Name:MIOX2
    Ordered Locus Names:At2g19800
    ORF Names:F6F22.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G19800.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Inositol oxygenase 2PRO_0000079155Add
    BLAST

    Proteomic databases

    PaxDbiO82200.
    PRIDEiO82200.

    Expressioni

    Tissue specificityi

    Expressed mainly in roots, stems, flowers and siliques. Low expression in leaves.1 Publication

    Gene expression databases

    ArrayExpressiO82200.
    GenevestigatoriO82200.

    Structurei

    3D structure databases

    ProteinModelPortaliO82200.
    SMRiO82200. Positions 56-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni115 – 1173Substrate bindingBy similarity
    Regioni174 – 1752Substrate bindingBy similarity
    Regioni252 – 2532Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the myo-inositol oxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG135479.
    HOGENOMiHOG000163182.
    InParanoidiO82200.
    KOiK00469.
    OMAiNGESERQ.
    PhylomeDBiO82200.

    Family and domain databases

    InterProiIPR007828. Inositol_oxygenase.
    [Graphical view]
    PANTHERiPTHR12588. PTHR12588. 1 hit.
    PfamiPF05153. DUF706. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O82200-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTILVEHFVP DSRVDEKKVI EERDNELVLD GGFVVPKSKE TDAFDAPDMN    50
    FLGHSFRDYE NGESERQQGV EEFYRMQHIH QTYDFVKKMR KEYGKLNKME 100
    MSIWECCELL NNVVDESDPD LDEPQIQHLL QTAEAIRRDY PDEDWLHLTA 150
    LIHDLGKVLL LPEFGGLPQW AVVGDTFPVG CTFDSANIHH KYFKGNHDIN 200
    NPKYNTKNGV YTEGCGLDNV LMSWGHDDYM YLVAKKNGTT LPHAGLFIIR 250
    YHSFYPLHKA GAYTHLMNDE DRDDLKWLHV FNKYDLYSKS KVLVDVEQVK 300
    PYYISLINKY FPAKLKW 317
    Length:317
    Mass (Da):37,048
    Last modified:June 1, 2002 - v2
    Checksum:i857EF7C180F00662
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti62 – 621G → D in AAM63498. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005169 Genomic DNA. Translation: AAC62136.2.
    CP002685 Genomic DNA. Translation: AEC06927.1.
    AY086497 mRNA. Translation: AAM63498.1.
    AF370587 mRNA. Translation: AAK43906.1.
    PIRiC84581.
    RefSeqiNP_565459.1. NM_127538.3.
    UniGeneiAt.12894.

    Genome annotation databases

    EnsemblPlantsiAT2G19800.1; AT2G19800.1; AT2G19800.
    GeneIDi816499.
    KEGGiath:AT2G19800.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005169 Genomic DNA. Translation: AAC62136.2 .
    CP002685 Genomic DNA. Translation: AEC06927.1 .
    AY086497 mRNA. Translation: AAM63498.1 .
    AF370587 mRNA. Translation: AAK43906.1 .
    PIRi C84581.
    RefSeqi NP_565459.1. NM_127538.3.
    UniGenei At.12894.

    3D structure databases

    ProteinModelPortali O82200.
    SMRi O82200. Positions 56-317.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi O82200.
    PRIDEi O82200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G19800.1 ; AT2G19800.1 ; AT2G19800 .
    GeneIDi 816499.
    KEGGi ath:AT2G19800.

    Organism-specific databases

    TAIRi AT2G19800.

    Phylogenomic databases

    eggNOGi NOG135479.
    HOGENOMi HOG000163182.
    InParanoidi O82200.
    KOi K00469.
    OMAi NGESERQ.
    PhylomeDBi O82200.

    Enzyme and pathway databases

    UniPathwayi UPA00111 ; UER00527 .

    Gene expression databases

    ArrayExpressi O82200.
    Genevestigatori O82200.

    Family and domain databases

    InterProi IPR007828. Inositol_oxygenase.
    [Graphical view ]
    PANTHERi PTHR12588. PTHR12588. 1 hit.
    Pfami PF05153. DUF706. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
      Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
      Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 76-317.
      Strain: cv. Columbia.
    6. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
      Lorence A., Chevone B.I., Mendes P., Nessler C.L.
      Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMIOX2_ARATH
    AccessioniPrimary (citable) accession number: O82200
    Secondary accession number(s): Q8LCN3, Q94JX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3