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Protein

Inositol oxygenase 2

Gene

MIOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis.2 Publications

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathway: myo-inositol degradation into D-glucuronate

This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol.
Proteins known to be involved in this subpathway in this organism are:
  1. Inositol oxygenase 2 (MIOX2), Inositol oxygenase 4 (MIOX4), Inositol oxygenase 1 (MIOX1), Inositol oxygenase 5 (MIOX5)
This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571SubstrateBy similarity
Metal bindingi128 – 1281Iron 1By similarity
Metal bindingi153 – 1531Iron 1By similarity
Metal bindingi154 – 1541Iron 1By similarity
Metal bindingi154 – 1541Iron 2By similarity
Binding sitei157 – 1571SubstrateBy similarity
Metal bindingi226 – 2261Iron 2By similarity
Metal bindingi252 – 2521Iron 2By similarity
Metal bindingi285 – 2851Iron 1By similarity

GO - Molecular functioni

  • inositol oxygenase activity Source: TAIR
  • iron ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.99.1. 399.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase 2 (EC:1.13.99.1)
Alternative name(s):
Myo-inositol oxygenase 2
Short name:
AtMIOX2
Short name:
MI oxygenase 2
Gene namesi
Name:MIOX2
Ordered Locus Names:At2g19800
ORF Names:F6F22.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G19800.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Inositol oxygenase 2PRO_0000079155Add
BLAST

Proteomic databases

PaxDbiO82200.
PRIDEiO82200.

Expressioni

Tissue specificityi

Expressed mainly in roots, stems, flowers and siliques. Low expression in leaves.1 Publication

Gene expression databases

ExpressionAtlasiO82200. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G19800.1.

Structurei

3D structure databases

ProteinModelPortaliO82200.
SMRiO82200. Positions 56-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 1173Substrate bindingBy similarity
Regioni174 – 1752Substrate bindingBy similarity
Regioni252 – 2532Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
InParanoidiO82200.
KOiK00469.
OMAiDSANIHH.
PhylomeDBiO82200.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTILVEHFVP DSRVDEKKVI EERDNELVLD GGFVVPKSKE TDAFDAPDMN
60 70 80 90 100
FLGHSFRDYE NGESERQQGV EEFYRMQHIH QTYDFVKKMR KEYGKLNKME
110 120 130 140 150
MSIWECCELL NNVVDESDPD LDEPQIQHLL QTAEAIRRDY PDEDWLHLTA
160 170 180 190 200
LIHDLGKVLL LPEFGGLPQW AVVGDTFPVG CTFDSANIHH KYFKGNHDIN
210 220 230 240 250
NPKYNTKNGV YTEGCGLDNV LMSWGHDDYM YLVAKKNGTT LPHAGLFIIR
260 270 280 290 300
YHSFYPLHKA GAYTHLMNDE DRDDLKWLHV FNKYDLYSKS KVLVDVEQVK
310
PYYISLINKY FPAKLKW
Length:317
Mass (Da):37,048
Last modified:June 1, 2002 - v2
Checksum:i857EF7C180F00662
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621G → D in AAM63498 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62136.2.
CP002685 Genomic DNA. Translation: AEC06927.1.
AY086497 mRNA. Translation: AAM63498.1.
AF370587 mRNA. Translation: AAK43906.1.
PIRiC84581.
RefSeqiNP_565459.1. NM_127538.3.
UniGeneiAt.12894.

Genome annotation databases

EnsemblPlantsiAT2G19800.1; AT2G19800.1; AT2G19800.
GeneIDi816499.
KEGGiath:AT2G19800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005169 Genomic DNA. Translation: AAC62136.2.
CP002685 Genomic DNA. Translation: AEC06927.1.
AY086497 mRNA. Translation: AAM63498.1.
AF370587 mRNA. Translation: AAK43906.1.
PIRiC84581.
RefSeqiNP_565459.1. NM_127538.3.
UniGeneiAt.12894.

3D structure databases

ProteinModelPortaliO82200.
SMRiO82200. Positions 56-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G19800.1.

Proteomic databases

PaxDbiO82200.
PRIDEiO82200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G19800.1; AT2G19800.1; AT2G19800.
GeneIDi816499.
KEGGiath:AT2G19800.

Organism-specific databases

TAIRiAT2G19800.

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
InParanoidiO82200.
KOiK00469.
OMAiDSANIHH.
PhylomeDBiO82200.

Enzyme and pathway databases

UniPathwayiUPA00111; UER00527.
BRENDAi1.13.99.1. 399.

Miscellaneous databases

PROiO82200.

Gene expression databases

ExpressionAtlasiO82200. baseline and differential.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides."
    Kanter U., Usadel B., Guerineau F., Li Y., Pauly M., Tenhaken R.
    Planta 221:243-254(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 76-317.
    Strain: cv. Columbia.
  6. "Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis."
    Lorence A., Chevone B.I., Mendes P., Nessler C.L.
    Plant Physiol. 134:1200-1205(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMIOX2_ARATH
AccessioniPrimary (citable) accession number: O82200
Secondary accession number(s): Q8LCN3, Q94JX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.