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Protein

NADP-dependent malic enzyme 1

Gene

NADP-ME1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.1 Publication
Oxaloacetate = pyruvate + CO2.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Kineticsi

kcat is 38.7 sec(-1) with NADP as substrate (at pH 7.5).
  1. KM=205 µM for NADP (at pH 7.5)1 Publication
  2. KM=2.96 mM for malate (at pH 7.5)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei129Proton donorBy similarity1
    Binding sitei182NADPBy similarity1
    Active sitei200Proton acceptorBy similarity1
    Metal bindingi272Divalent metal cationBy similarity1
    Metal bindingi273Divalent metal cationBy similarity1
    Metal bindingi296Divalent metal cationBy similarity1
    Binding sitei296NADPBy similarity1
    Sitei296Important for activityBy similarity1
    Binding sitei437NADPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi325 – 341NADPBy similarityAdd BLAST17

    GO - Molecular functioni

    GO - Biological processi

    • malate metabolic process Source: TAIR
    • protein homooligomerization Source: TAIR
    • pyruvate metabolic process Source: GO_Central

    Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G19900-MONOMER
    ReactomeiR-ATH-70268 Pyruvate metabolism
    R-ATH-71403 Citric acid cycle (TCA cycle)
    SABIO-RKO82191

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-dependent malic enzyme 1 (EC:1.1.1.40)
    Short name:
    AtNADP-ME1
    Short name:
    NADP-malic enzyme 1
    Gene namesi
    Name:NADP-ME1
    Ordered Locus Names:At2g19900
    ORF Names:F6F22.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G19900
    TAIRilocus:2052045 AT2G19900

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004201491 – 581NADP-dependent malic enzyme 1Add BLAST581

    Proteomic databases

    PaxDbiO82191
    PRIDEiO82191

    PTM databases

    iPTMnetiO82191

    Expressioni

    Tissue specificityi

    Specifically expressed in roots (only in steles of secondary roots).1 Publication

    Developmental stagei

    During embryogenesis, present only in the embryo from the torpedo stage onward. During germination, first restricted to the radicle to later become more pronounced in the root tip. Expressed in hypocotyl and cotyledons 5 days after imbibition.1 Publication

    Gene expression databases

    ExpressionAtlasiO82191 baseline and differential
    GenevisibleiO82191 AT

    Interactioni

    Subunit structurei

    Homohexamers and homooctamers.1 Publication

    Protein-protein interaction databases

    BioGridi1864, 2 interactors
    IntActiO82191, 1 interactor
    STRINGi3702.AT2G19900.1

    Structurei

    3D structure databases

    ProteinModelPortaliO82191
    SMRiO82191
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malic enzymes family.Curated

    Phylogenomic databases

    eggNOGiKOG1257 Eukaryota
    COG0281 LUCA
    HOGENOMiHOG000042486
    InParanoidiO82191
    KOiK00029
    OMAiVKCAESS
    OrthoDBiEOG093606RR
    PhylomeDBiO82191

    Family and domain databases

    Gene3Di3.40.50.10380, 1 hit
    InterProiView protein in InterPro
    IPR015884 Malic_enzyme_CS
    IPR012301 Malic_N_dom
    IPR037062 Malic_N_dom_sf
    IPR012302 Malic_NAD-bd
    IPR001891 Malic_OxRdtase
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF00390 malic, 1 hit
    PF03949 Malic_M, 1 hit
    PIRSFiPIRSF000106 ME, 1 hit
    PRINTSiPR00072 MALOXRDTASE
    SMARTiView protein in SMART
    SM01274 malic, 1 hit
    SM00919 Malic_M, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00331 MALIC_ENZYMES, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O82191-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKVTNSDLK SSVDGGVVDV YGEDSATIEH NITPWSLSVS SGYSLLRDPR
    60 70 80 90 100
    YNKGLAFTEK ERDTHYLRGL LPPVVLDQKL QEKRLLNNIR QYQFPLQKYM
    110 120 130 140 150
    ALTELQERNE RLFYKLLIDN VEELLPIVYT PTVGEACQKF GSIFRRPQGL
    160 170 180 190 200
    FISLKDKGKI LDVLKNWPER NIQVIVVTDG ERILGLGDLG CQGMGIPVGK
    210 220 230 240 250
    LALYSALGGV RPSACLPVTI DVGTNNEKLL NDEFYIGLRQ KRATGQEYSE
    260 270 280 290 300
    LLNEFMSAVK QNYGEKVLIQ FEDFANHNAF ELLAKYSDTH LVFNDDIQGT
    310 320 330 340 350
    ASVVLAGLVS AQKLTNSPLA EHTFLFLGAG EAGTGIAELI ALYMSKQMNA
    360 370 380 390 400
    SVEESRKKIW LVDSKGLIVN SRKDSLQDFK KPWAHEHEPV KDLLGAIKAI
    410 420 430 440 450
    KPTVLIGSSG VGRSFTKEVI EAMSSINERP LIMALSNPTT QSECTAEEAY
    460 470 480 490 500
    TWSKGRAIFA SGSPFDPVEY EGKVFVSTQA NNAYIFPGFG LGLVISGAIR
    510 520 530 540 550
    VHDDMLLAAA EALAGQVSKE NYEKGMIYPS FSSIRKISAQ IAANVATKAY
    560 570 580
    ELGLAGRLPR PKDIVKCAES SMYSPTYRLY R
    Length:581
    Mass (Da):64,279
    Last modified:November 1, 1998 - v1
    Checksum:i0F7EB58E2C6968C2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005169 Genomic DNA Translation: AAC62126.1
    CP002685 Genomic DNA Translation: AEC06941.1
    AY062734 mRNA Translation: AAL32812.1
    BT003371 mRNA Translation: AAO30034.1
    PIRiE84582
    RefSeqiNP_179580.1, NM_127548.4
    UniGeneiAt.27251

    Genome annotation databases

    EnsemblPlantsiAT2G19900.1; AT2G19900.1; AT2G19900
    GeneIDi816509
    GrameneiAT2G19900.1; AT2G19900.1; AT2G19900
    KEGGiath:AT2G19900

    Similar proteinsi

    Entry informationi

    Entry nameiMAOP1_ARATH
    AccessioniPrimary (citable) accession number: O82191
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: November 1, 1998
    Last modified: May 23, 2018
    This is version 127 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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