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Protein

NADP-dependent malic enzyme 1

Gene

NADP-ME1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.1 Publication
Oxaloacetate = pyruvate + CO2.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Kineticsi

kcat is 38.7 sec(-1) with NADP as substrate (at pH 7.5).

  1. KM=205 µM for NADP (at pH 7.5)1 Publication
  2. KM=2.96 mM for malate (at pH 7.5)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei129Proton donorBy similarity1
    Binding sitei182NADPBy similarity1
    Active sitei200Proton acceptorBy similarity1
    Metal bindingi272Divalent metal cationBy similarity1
    Metal bindingi273Divalent metal cationBy similarity1
    Metal bindingi296Divalent metal cationBy similarity1
    Binding sitei296NADPBy similarity1
    Sitei296Important for activityBy similarity1
    Binding sitei437NADPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi325 – 341NADPBy similarityAdd BLAST17

    GO - Molecular functioni

    GO - Biological processi

    • malate metabolic process Source: TAIR
    • protein homooligomerization Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G19900-MONOMER.
    SABIO-RKO82191.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-dependent malic enzyme 1 (EC:1.1.1.40)
    Short name:
    AtNADP-ME1
    Short name:
    NADP-malic enzyme 1
    Gene namesi
    Name:NADP-ME1
    Ordered Locus Names:At2g19900
    ORF Names:F6F22.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G19900.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004201491 – 581NADP-dependent malic enzyme 1Add BLAST581

    Proteomic databases

    PaxDbiO82191.
    PRIDEiO82191.

    PTM databases

    iPTMnetiO82191.

    Expressioni

    Tissue specificityi

    Specifically expressed in roots (only in steles of secondary roots).1 Publication

    Developmental stagei

    During embryogenesis, present only in the embryo from the torpedo stage onward. During germination, first restricted to the radicle to later become more pronounced in the root tip. Expressed in hypocotyl and cotyledons 5 days after imbibition.1 Publication

    Gene expression databases

    GenevisibleiO82191. AT.

    Interactioni

    Subunit structurei

    Homohexamers and homooctamers.1 Publication

    Protein-protein interaction databases

    BioGridi1864. 2 interactors.
    IntActiO82191. 1 interactor.
    STRINGi3702.AT2G19900.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO82191.
    SMRiO82191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malic enzymes family.Curated

    Phylogenomic databases

    eggNOGiKOG1257. Eukaryota.
    COG0281. LUCA.
    HOGENOMiHOG000042486.
    InParanoidiO82191.
    KOiK00029.
    OMAiPEDLFKW.
    OrthoDBiEOG093606RR.
    PhylomeDBiO82191.

    Family and domain databases

    Gene3Di3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000106. ME. 1 hit.
    PRINTSiPR00072. MALOXRDTASE.
    SMARTiSM01274. malic. 1 hit.
    SM00919. Malic_M. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O82191-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKVTNSDLK SSVDGGVVDV YGEDSATIEH NITPWSLSVS SGYSLLRDPR
    60 70 80 90 100
    YNKGLAFTEK ERDTHYLRGL LPPVVLDQKL QEKRLLNNIR QYQFPLQKYM
    110 120 130 140 150
    ALTELQERNE RLFYKLLIDN VEELLPIVYT PTVGEACQKF GSIFRRPQGL
    160 170 180 190 200
    FISLKDKGKI LDVLKNWPER NIQVIVVTDG ERILGLGDLG CQGMGIPVGK
    210 220 230 240 250
    LALYSALGGV RPSACLPVTI DVGTNNEKLL NDEFYIGLRQ KRATGQEYSE
    260 270 280 290 300
    LLNEFMSAVK QNYGEKVLIQ FEDFANHNAF ELLAKYSDTH LVFNDDIQGT
    310 320 330 340 350
    ASVVLAGLVS AQKLTNSPLA EHTFLFLGAG EAGTGIAELI ALYMSKQMNA
    360 370 380 390 400
    SVEESRKKIW LVDSKGLIVN SRKDSLQDFK KPWAHEHEPV KDLLGAIKAI
    410 420 430 440 450
    KPTVLIGSSG VGRSFTKEVI EAMSSINERP LIMALSNPTT QSECTAEEAY
    460 470 480 490 500
    TWSKGRAIFA SGSPFDPVEY EGKVFVSTQA NNAYIFPGFG LGLVISGAIR
    510 520 530 540 550
    VHDDMLLAAA EALAGQVSKE NYEKGMIYPS FSSIRKISAQ IAANVATKAY
    560 570 580
    ELGLAGRLPR PKDIVKCAES SMYSPTYRLY R
    Length:581
    Mass (Da):64,279
    Last modified:November 1, 1998 - v1
    Checksum:i0F7EB58E2C6968C2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005169 Genomic DNA. Translation: AAC62126.1.
    CP002685 Genomic DNA. Translation: AEC06941.1.
    AY062734 mRNA. Translation: AAL32812.1.
    BT003371 mRNA. Translation: AAO30034.1.
    PIRiE84582.
    RefSeqiNP_179580.1. NM_127548.4.
    UniGeneiAt.27251.

    Genome annotation databases

    EnsemblPlantsiAT2G19900.1; AT2G19900.1; AT2G19900.
    GeneIDi816509.
    GrameneiAT2G19900.1; AT2G19900.1; AT2G19900.
    KEGGiath:AT2G19900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC005169 Genomic DNA. Translation: AAC62126.1.
    CP002685 Genomic DNA. Translation: AEC06941.1.
    AY062734 mRNA. Translation: AAL32812.1.
    BT003371 mRNA. Translation: AAO30034.1.
    PIRiE84582.
    RefSeqiNP_179580.1. NM_127548.4.
    UniGeneiAt.27251.

    3D structure databases

    ProteinModelPortaliO82191.
    SMRiO82191.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi1864. 2 interactors.
    IntActiO82191. 1 interactor.
    STRINGi3702.AT2G19900.1.

    PTM databases

    iPTMnetiO82191.

    Proteomic databases

    PaxDbiO82191.
    PRIDEiO82191.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G19900.1; AT2G19900.1; AT2G19900.
    GeneIDi816509.
    GrameneiAT2G19900.1; AT2G19900.1; AT2G19900.
    KEGGiath:AT2G19900.

    Organism-specific databases

    TAIRiAT2G19900.

    Phylogenomic databases

    eggNOGiKOG1257. Eukaryota.
    COG0281. LUCA.
    HOGENOMiHOG000042486.
    InParanoidiO82191.
    KOiK00029.
    OMAiPEDLFKW.
    OrthoDBiEOG093606RR.
    PhylomeDBiO82191.

    Enzyme and pathway databases

    BioCyciARA:AT2G19900-MONOMER.
    SABIO-RKO82191.

    Miscellaneous databases

    PROiO82191.

    Gene expression databases

    GenevisibleiO82191. AT.

    Family and domain databases

    Gene3Di3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000106. ME. 1 hit.
    PRINTSiPR00072. MALOXRDTASE.
    SMARTiSM01274. malic. 1 hit.
    SM00919. Malic_M. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMAOP1_ARATH
    AccessioniPrimary (citable) accession number: O82191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: November 1, 1998
    Last modified: November 30, 2016
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.