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Protein

Glycine cleavage system H protein 2, mitochondrial

Gene

GDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine decarboxylase (GDC) or glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).By similarity

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

GO - Molecular functioni

  • ATP binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciARA:AT2G35120-MONOMER.
MetaCyc:MONOMER-907.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein 2, mitochondrial
Gene namesi
Name:GDH2
Ordered Locus Names:At2g35120
ORF Names:T4C15.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G35120.

Subcellular locationi

GO - Cellular componenti

  • glycine cleavage complex Source: InterPro
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2323MitochondrionSequence analysisAdd
BLAST
Chaini24 – 156133Glycine cleavage system H protein 2, mitochondrialPRO_0000422286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881N6-lipoyllysinePROSITE-ProRule annotationBy similarity
Modified residuei131 – 1311PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO82179.
PRIDEiO82179.

Expressioni

Gene expression databases

GenevisibleiO82179. AT.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

STRINGi3702.AT2G35120.1.

Structurei

3D structure databases

ProteinModelPortaliO82179.
SMRiO82179. Positions 26-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 12983Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GcvH family.Curated
Contains 1 lipoyl-binding domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
HOGENOMiHOG000239392.
KOiK02437.
OMAiHEWVELV.
PhylomeDBiO82179.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O82179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACRLFWASR VASHLRISVA QRGFSSVVLK DLKYADSHEW VKIDGNKATF
60 70 80 90 100
GITDHAQDHL GDVVYVELPD VGHSVSQGKS FGAVESVKAT SDINSPVSGK
110 120 130 140 150
VVEVNEELTE SPGLVNSSPY EQGWIIKVEL SDAGEAEKLM DSDKYSKFCE

EEDAKH
Length:156
Mass (Da):17,100
Last modified:November 1, 1998 - v1
Checksum:iA8098D7174695749
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004667 Genomic DNA. Translation: AAC61829.1.
CP002685 Genomic DNA. Translation: AEC09067.1.
AY056106 mRNA. Translation: AAL06993.1.
AY060493 mRNA. Translation: AAL31106.1.
AK227751 mRNA. Translation: BAE99735.1.
PIRiH84764.
RefSeqiNP_181057.1. NM_129065.3.
UniGeneiAt.12721.

Genome annotation databases

EnsemblPlantsiAT2G35120.1; AT2G35120.1; AT2G35120.
GeneIDi818078.
GrameneiAT2G35120.1; AT2G35120.1; AT2G35120.
KEGGiath:AT2G35120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004667 Genomic DNA. Translation: AAC61829.1.
CP002685 Genomic DNA. Translation: AEC09067.1.
AY056106 mRNA. Translation: AAL06993.1.
AY060493 mRNA. Translation: AAL31106.1.
AK227751 mRNA. Translation: BAE99735.1.
PIRiH84764.
RefSeqiNP_181057.1. NM_129065.3.
UniGeneiAt.12721.

3D structure databases

ProteinModelPortaliO82179.
SMRiO82179. Positions 26-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G35120.1.

Proteomic databases

PaxDbiO82179.
PRIDEiO82179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G35120.1; AT2G35120.1; AT2G35120.
GeneIDi818078.
GrameneiAT2G35120.1; AT2G35120.1; AT2G35120.
KEGGiath:AT2G35120.

Organism-specific databases

TAIRiAT2G35120.

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
HOGENOMiHOG000239392.
KOiK02437.
OMAiHEWVELV.
PhylomeDBiO82179.

Enzyme and pathway databases

BioCyciARA:AT2G35120-MONOMER.
MetaCyc:MONOMER-907.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-6783984. Glycine degradation.

Miscellaneous databases

PROiO82179.

Gene expression databases

GenevisibleiO82179. AT.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The glycine decarboxylase system: a fascinating complex."
    Douce R., Bourguignon J., Neuburger M., Rebeille F.
    Trends Plant Sci. 6:167-176(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Genetic manipulation of glycine decarboxylation."
    Bauwe H., Kolukisaoglu U.
    J. Exp. Bot. 54:1523-1535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, NOMENCLATURE.
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGCSH2_ARATH
AccessioniPrimary (citable) accession number: O82179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.