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Protein

ADP-ribosylation factor GTPase-activating protein AGD10

Gene

AGD10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Activates ARF1 and ARF2. Required for female gametophyte development. Involved in root hair and pollen tube growth.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • pollen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein AGD10
Short name:
ARF GAP AGD10
Alternative name(s):
Protein ARF-GAP DOMAIN 10
Short name:
AtAGD10
Protein MATERNAL EFFECT EMBRYO ARREST 28
Protein ROOT AND POLLEN ARFGAP
Gene namesi
Name:AGD10
Synonyms:MEE28, RPA
Ordered Locus Names:At2g35210
ORF Names:T4C15.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G35210.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

Plants are arrested during endosperm development and have altered root hair development and pollen tube elongation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 395394ADP-ribosylation factor GTPase-activating protein AGD10PRO_0000352501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei307 – 3071PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO82171.
PRIDEiO82171.

PTM databases

iPTMnetiO82171.

Expressioni

Tissue specificityi

Expressed specifically in roots, pollen grains and pollen tubes.1 Publication

Gene expression databases

GenevisibleiO82171. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G35210.1.

Structurei

3D structure databases

ProteinModelPortaliO82171.
SMRiO82171. Positions 11-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 128119Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili361 – 38929Sequence analysisAdd
BLAST

Domaini

The C-terminal domain (317-395) is responsible for the Golgi localization.

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 4824C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000162970.
InParanoidiO82171.
KOiK12493.
OMAiREDYMSP.
PhylomeDBiO82171.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O82171-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASENLNDKI SVFKKLKAKS DNKICFDCNA KNPTWASVTY GIFLCIDCSA
60 70 80 90 100
VHRSLGVHIS FVRSTNLDSW SSEQLKMMIY GGNNRAQVFF KQYGWSDGGK
110 120 130 140 150
TEAKYTSRAA DLYKQILAKE VAKSKAEEEL DLPPSPPDST QVPNGLSSIK
160 170 180 190 200
TSEALKESNT LKQQEKPDVV PVSPRISRSV KKPLGAKKTG KTGGLGARKL
210 220 230 240 250
TTKSSGTLYD QKPEESVIIQ ATSPVSAKSA RSSFSSRFDY ADNVQNREDY
260 270 280 290 300
MSPQVVSHVA PPKSSGFFEE ELEMNGGRFQ KKPITSSSKL QIQETDEARK
310 320 330 340 350
KFTNAKSISS AQYFGNDNNS ADLEAKSSLK KFSGSSAISS ADLFGDGDGD
360 370 380 390
FPLDLTAGDL LNRLSLQAQQ DISSLKNMAE ETKKKLGSVA SSLWV
Length:395
Mass (Da):43,097
Last modified:November 1, 1998 - v1
Checksum:iE387B8F8062D57B3
GO
Isoform 2 (identifier: O82171-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-371: GSSAISSADL...NRLSLQAQQD → QSLVLICLET...LSRHNKTYHH
     372-395: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:371
Mass (Da):41,057
Checksum:i93DD722323F8DB2C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei334 – 37138GSSAI…QAQQD → QSLVLICLETVMEISLLISL RVIFSTAYLSRHNKTYHH in isoform 2. CuratedVSP_035551Add
BLAST
Alternative sequencei372 – 39524Missing in isoform 2. CuratedVSP_035552Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004667 Genomic DNA. Translation: AAC61816.1.
CP002685 Genomic DNA. Translation: AEC09080.1.
CP002685 Genomic DNA. Translation: AEC09081.1.
AY085599 mRNA. Translation: AAM62820.1.
AK229368 mRNA. Translation: BAF01231.1.
PIRiH84765.
RefSeqiNP_565801.1. NM_129074.2. [O82171-1]
NP_973603.1. NM_201874.2. [O82171-2]
UniGeneiAt.37696.

Genome annotation databases

EnsemblPlantsiAT2G35210.1; AT2G35210.1; AT2G35210. [O82171-1]
GeneIDi818088.
KEGGiath:AT2G35210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004667 Genomic DNA. Translation: AAC61816.1.
CP002685 Genomic DNA. Translation: AEC09080.1.
CP002685 Genomic DNA. Translation: AEC09081.1.
AY085599 mRNA. Translation: AAM62820.1.
AK229368 mRNA. Translation: BAF01231.1.
PIRiH84765.
RefSeqiNP_565801.1. NM_129074.2. [O82171-1]
NP_973603.1. NM_201874.2. [O82171-2]
UniGeneiAt.37696.

3D structure databases

ProteinModelPortaliO82171.
SMRiO82171. Positions 11-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G35210.1.

PTM databases

iPTMnetiO82171.

Proteomic databases

PaxDbiO82171.
PRIDEiO82171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G35210.1; AT2G35210.1; AT2G35210. [O82171-1]
GeneIDi818088.
KEGGiath:AT2G35210.

Organism-specific databases

TAIRiAT2G35210.

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000162970.
InParanoidiO82171.
KOiK12493.
OMAiREDYMSP.
PhylomeDBiO82171.

Enzyme and pathway databases

ReactomeiR-ATH-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiO82171.

Gene expression databases

GenevisibleiO82171. AT.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Analysis of the small GTPase gene superfamily of Arabidopsis."
    Vernoud V., Horton A.C., Yang Z., Nielsen E.
    Plant Physiol. 131:1191-1208(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
    Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
    Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "RPA, a class II ARFGAP protein, activates ARF1 and U5 and plays a role in root hair development in Arabidopsis."
    Song X.-F., Yang C.-Y., Liu J., Yang W.-C.
    Plant Physiol. 141:966-976(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiAGD10_ARATH
AccessioniPrimary (citable) accession number: O82171
Secondary accession number(s): Q3EBM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.