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O82144 (CHSY_HYDMC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase
Gene names
Name:CHS
OrganismHydrangea macrophylla (Bigleaf hydrangea)
Taxonomic identifier23110 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsCornalesHydrangeaceaeHydrangea

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Chalcone synthase
PRO_0000215981

Sites

Active site1641 By similarity

Sequences

Sequence LengthMass (Da)Tools
O82144 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 187FC9CB9F33F568

FASTA38942,635
        10         20         30         40         50         60 
MVTVEEVRKA QRAEGPATIL AIGTATPPNY VDQSTYPDFY FRVTNSEHKK ELKAKFQRMC 

        70         80         90        100        110        120 
DNSQIKKRYM HLTEEILKEN PNICAYMAPS LDARQDMVVV EIPKLGKEAA TRAIKEWGQP 

       130        140        150        160        170        180 
KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN 

       190        200        210        220        230        240 
NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIIGSDPMP EVEKPLFEIV 

       250        260        270        280        290        300 
SAAQTILPDS DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLVEAFRPL DISDWNSIFW 

       310        320        330        340        350        360 
IAHPGGPAIL DQVEKKLALK PEKLRATRNV LSDYGNMSSA CVLFIMDEMR KNSAEEGLMT 

       370        380 
TGEGLEWGVL FGFGPGLTVE TVVLHGVST 

« Hide

References

[1]"cDNA cloning of polyketide synthases from Hydrangea macrophylla var. thunbergii."
Akiyama T., Shibuya M., Ebizuka Y.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Var. Thunbergii.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB011467 mRNA. Translation: BAA32732.1.

3D structure databases

ProteinModelPortalO82144.
SMRO82144. Positions 3-388.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHSY_HYDMC
AccessionPrimary (citable) accession number: O82144
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways