Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chalcone synthase

Gene

CHS

Organism
Hydrangea macrophylla (Bigleaf hydrangea) (Viburnum macrophyllum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641PROSITE-ProRule annotation

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
Gene namesi
Name:CHS
OrganismiHydrangea macrophylla (Bigleaf hydrangea) (Viburnum macrophyllum)
Taxonomic identifieri23110 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsCornalesHydrangeaceaeHydrangea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Chalcone synthasePRO_0000215981Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO82144.
SMRiO82144. Positions 3-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O82144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVEEVRKA QRAEGPATIL AIGTATPPNY VDQSTYPDFY FRVTNSEHKK
60 70 80 90 100
ELKAKFQRMC DNSQIKKRYM HLTEEILKEN PNICAYMAPS LDARQDMVVV
110 120 130 140 150
EIPKLGKEAA TRAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL
160 170 180 190 200
RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG
210 220 230 240 250
PSDTHLDSLV GQALFGDGAA AVIIGSDPMP EVEKPLFEIV SAAQTILPDS
260 270 280 290 300
DGAIDGHLRE VGLTFHLLKD VPGLISKNIE KSLVEAFRPL DISDWNSIFW
310 320 330 340 350
IAHPGGPAIL DQVEKKLALK PEKLRATRNV LSDYGNMSSA CVLFIMDEMR
360 370 380
KNSAEEGLMT TGEGLEWGVL FGFGPGLTVE TVVLHGVST
Length:389
Mass (Da):42,635
Last modified:November 1, 1998 - v1
Checksum:i187FC9CB9F33F568
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011467 mRNA. Translation: BAA32732.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011467 mRNA. Translation: BAA32732.1.

3D structure databases

ProteinModelPortaliO82144.
SMRiO82144. Positions 3-388.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of polyketide synthases from Hydrangea macrophylla var. thunbergii."
    Akiyama T., Shibuya M., Ebizuka Y.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Var. Thunbergii.
    Tissue: Leaf.

Entry informationi

Entry nameiCHSY_HYDMC
AccessioniPrimary (citable) accession number: O82144
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: March 4, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.