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Protein

Dehydration-responsive element-binding protein 2A

Gene

DREB2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi78 – 13558AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: GO_Central
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • heat acclimation Source: TAIR
  • positive regulation of transcription, DNA-templated Source: GO_Central
  • response to heat Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to UV-B Source: TAIR
  • response to water deprivation Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydration-responsive element-binding protein 2A
Short name:
Protein DREB2A
Gene namesi
Name:DREB2A
Synonyms:ERF045
Ordered Locus Names:At5g05410
ORF Names:K18I23.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G05410.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi91 – 911V → A: Affects the binding to the CRT/DRE cis-element. 1 Publication
Mutagenesisi96 – 961E → D: Affects the binding to the CRT/DRE cis-element. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Dehydration-responsive element-binding protein 2APRO_0000112534Add
BLAST

Post-translational modificationi

Ubiquitinated by DRIP1 and DRIP2. Ubiquitination probably leads to its subsequent degradation, thus negatively regulating response to drought.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiO82132.
PRIDEiO82132.

Expressioni

Tissue specificityi

Expressed preferentially in roots and stems, and at a lower level in leaves.2 Publications

Inductioni

By high-salt and drought stresses.3 Publications

Gene expression databases

ExpressionAtlasiO82132. baseline and differential.
GenevisibleiO82132. AT.

Interactioni

Subunit structurei

Interacts with MED25.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DRIP1Q9M9Y44EBI-1786840,EBI-1786858
RCD1Q8RY593EBI-1786840,EBI-2118043

Protein-protein interaction databases

BioGridi15703. 9 interactions.
DIPiDIP-40571N.
IntActiO82132. 30 interactions.
STRINGi3702.AT5G05410.1.

Structurei

3D structure databases

ProteinModelPortaliO82132.
SMRiO82132. Positions 79-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi19 – 5537Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi230 – 2345Poly-Gln

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IXVH. Eukaryota.
ENOG410YKCQ. LUCA.
HOGENOMiHOG000015215.
InParanoidiO82132.
OMAiEVCTVET.
PhylomeDBiO82132.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O82132-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVYDQSGDR NRTQIDTSRK RKSRSRGDGT TVAERLKRWK EYNETVEEVS
60 70 80 90 100
TKKRKVPAKG SKKGCMKGKG GPENSRCSFR GVRQRIWGKW VAEIREPNRG
110 120 130 140 150
SRLWLGTFPT AQEAASAYDE AAKAMYGPLA RLNFPRSDAS EVTSTSSQSE
160 170 180 190 200
VCTVETPGCV HVKTEDPDCE SKPFSGGVEP MYCLENGAEE MKRGVKADKH
210 220 230 240 250
WLSEFEHNYW SDILKEKEKQ KEQGIVETCQ QQQQDSLSVA DYGWPNDVDQ
260 270 280 290 300
SHLDSSDMFD VDELLRDLNG DDVFAGLNQD RYPGNSVANG SYRPESQQSG
310 320 330
FDPLQSLNYG IPPFQLEGKD GNGFFDDLSY LDLEN
Length:335
Mass (Da):37,703
Last modified:November 1, 1998 - v1
Checksum:i9742CE579CC7314C
GO

Sequence cautioni

The sequence AAU93685.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621V → M in AAU93685 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007790 mRNA. Translation: BAA33794.1.
AB016570 Genomic DNA. Translation: BAA36705.1.
AB010692 Genomic DNA. Translation: BAB09984.1.
CP002688 Genomic DNA. Translation: AED90870.1.
AY063972 mRNA. Translation: AAL36328.1.
AY691903 Genomic DNA. Translation: AAU93685.1. Sequence problems.
PIRiT51833.
RefSeqiNP_196160.1. NM_120623.2. [O82132-1]
UniGeneiAt.25247.

Genome annotation databases

EnsemblPlantsiAT5G05410.1; AT5G05410.1; AT5G05410. [O82132-1]
GeneIDi830424.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007790 mRNA. Translation: BAA33794.1.
AB016570 Genomic DNA. Translation: BAA36705.1.
AB010692 Genomic DNA. Translation: BAB09984.1.
CP002688 Genomic DNA. Translation: AED90870.1.
AY063972 mRNA. Translation: AAL36328.1.
AY691903 Genomic DNA. Translation: AAU93685.1. Sequence problems.
PIRiT51833.
RefSeqiNP_196160.1. NM_120623.2. [O82132-1]
UniGeneiAt.25247.

3D structure databases

ProteinModelPortaliO82132.
SMRiO82132. Positions 79-135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15703. 9 interactions.
DIPiDIP-40571N.
IntActiO82132. 30 interactions.
STRINGi3702.AT5G05410.1.

Proteomic databases

PaxDbiO82132.
PRIDEiO82132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G05410.1; AT5G05410.1; AT5G05410. [O82132-1]
GeneIDi830424.

Organism-specific databases

TAIRiAT5G05410.

Phylogenomic databases

eggNOGiENOG410IXVH. Eukaryota.
ENOG410YKCQ. LUCA.
HOGENOMiHOG000015215.
InParanoidiO82132.
OMAiEVCTVET.
PhylomeDBiO82132.

Miscellaneous databases

PROiO82132.

Gene expression databases

ExpressionAtlasiO82132. baseline and differential.
GenevisibleiO82132. AT.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis."
    Liu Q., Kasuga M., Sakuma Y., Abe H., Miura S., Yamaguchi-Shinozaki K., Shinozaki K.
    Plant Cell 10:1391-1406(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression."
    Nakashima K., Shinwari Z.K., Sakuma Y., Seki M., Miura S., Shinozaki K., Yamaguchi-Shinozaki K.
    Plant Mol. Biol. 42:657-665(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. Zhou R.Y., Sun Z.X.
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-167.
  7. "DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression."
    Sakuma Y., Liu Q., Dubouzet J.G., Abe H., Shinozaki K., Yamaguchi-Shinozaki K.
    Biochem. Biophys. Res. Commun. 290:998-1009(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, FUNCTION, INDUCTION, MUTAGENESIS OF VAL-91 AND GLU-96.
  8. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Arabidopsis DREB2A-interacting proteins function as RING E3 ligases and negatively regulate plant drought stress-responsive gene expression."
    Qin F., Sakuma Y., Tran L.-S.H., Maruyama K., Kidokoro S., Fujita Y., Fujita M., Umezawa T., Sawano Y., Miyazono K., Tanokura M., Shinozaki K., Yamaguchi-Shinozaki K.
    Plant Cell 20:1693-1707(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DRIP1 AND DRIP2, SUBCELLULAR LOCATION, UBIQUITINATION.
  10. "The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development."
    Elfving N., Davoine C., Benlloch R., Blomberg J., Braennstroem K., Mueller D., Nilsson A., Ulfstedt M., Ronne H., Wingsle G., Nilsson O., Bjoerklund S.
    Proc. Natl. Acad. Sci. U.S.A. 108:8245-8250(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED25.

Entry informationi

Entry nameiDRE2A_ARATH
AccessioniPrimary (citable) accession number: O82132
Secondary accession number(s): Q5Y4C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.