ID   ILV5_PEA                Reviewed;         581 AA.
AC   O82043;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Ketol-acid reductoisomerase, chloroplastic;
DE            EC=1.1.1.86;
DE   AltName: Full=Acetohydroxy-acid reductoisomerase;
DE   AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase;
DE   Flags: Precursor;
GN   Name=PGAAIR;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. G2;
RA   Gu X., Xu Y., Wu J., Hou X., Chen Z., Zhu Y.;
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+)
CC       = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CC   -!- CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate +
CC       NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 2/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 2/4.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the ketol-acid reductoisomerase family.
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DR   EMBL; Y17796; CAA76854.1; -; mRNA.
DR   PIR; T06825; T06825.
DR   HSSP; Q01292; 1QMG.
DR   SMR; O82043; 69-578.
DR   BRENDA; 1.1.1.86; 287.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004455; F:ketol-acid reductoisomerase activity; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009082; P:branched chain family amino acid biosynthet...; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013023; AcH_isomrdctse.
DR   InterPro; IPR000506; AcH_isomrdctse_C.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR013116; IlvN.
DR   InterPro; IPR016206; KetolA_reductoisomerase_pln.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR21371; AcH_isomrdctse; 1.
DR   Pfam; PF01450; IlvC; 1.
DR   Pfam; PF07991; IlvN; 1.
DR   PIRSF; PIRSF000118; Ilv5_plant; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Chloroplast; Magnesium; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     58       Chloroplast (By similarity).
FT   CHAIN        59    581       Ketol-acid reductoisomerase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000015630.
FT   ACT_SITE    210    210       Potential.
SQ   SEQUENCE   581 AA;  62852 MW;  D6F2B8DF4B2AC697 CRC64;
     MAAVTSSCST AISASSKTLA KPVAASFAPT NLSFSKLSPQ SIRARRSITV GSALGATKVS
     APPATHPVSL DFETSVFKKE RVNLAGHEEY IVRGGRDLFH LLPDAFKGIK QIGVIGWGSQ
     GPAQAQNLRD SLVEAKSDIV VKVGLRKGSS SFNEAREAGF SEEKGTLGDI WETISGSDLV
     LLLISDSAQA DNYEKIFSHL KPNSILGLSH GFLLGHLQSI GLDFPKNFSV IAVCPKGMGP
     SVRRLYVQGK EINGAGINSS FGVHQDVDGR ATNVALGWSV ALGSPFTFAT TLEQEYKSDI
     FGERGILLGA VHGIVESLFR RYTENGMSED LAYKNTVESI TGVISKTIST QGMLAVYNAL
     SEDGKKEFEK AYSASFYPCM EILYECYEDV ASGSEIRSVV LAGRRFYEKE GLPAFPMGKI
     DQTRMWKVGE RVRSTRPAGD LGPLYPFTAG VFVAMMMAQI EVLRKKGHSY SEIINESVIE
     SVDSLNPFMH ARGVSFMVDN CSTTARLGSR KWAPRFDYIL TQQALVAVDS GAPINQDLIS
     NFVSDPVHGA IQVCAELRPT LDISVPAAAD FVRPELRQCS N
//