Reviewed, UniProtKB/Swiss-Prot O82043 (ILV5_PEA)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase, chloroplastic
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid reductoisomerase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:

PGAAIR

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Cofactor	Magnesium By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast By similarity
	Chain	59 – 581	523	Ketol-acid reductoisomerase, chloroplastic		PRO_0000015630
Sites
	Active site	210	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O82043-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: D6F2B8DF4B2AC697	FASTA	581	62,852
	10 20 30 40 50 60 MAAVTSSCST AISASSKTLA KPVAASFAPT NLSFSKLSPQ SIRARRSITV GSALGATKVS 70 80 90 100 110 120 APPATHPVSL DFETSVFKKE RVNLAGHEEY IVRGGRDLFH LLPDAFKGIK QIGVIGWGSQ 130 140 150 160 170 180 GPAQAQNLRD SLVEAKSDIV VKVGLRKGSS SFNEAREAGF SEEKGTLGDI WETISGSDLV 190 200 210 220 230 240 LLLISDSAQA DNYEKIFSHL KPNSILGLSH GFLLGHLQSI GLDFPKNFSV IAVCPKGMGP 250 260 270 280 290 300 SVRRLYVQGK EINGAGINSS FGVHQDVDGR ATNVALGWSV ALGSPFTFAT TLEQEYKSDI 310 320 330 340 350 360 FGERGILLGA VHGIVESLFR RYTENGMSED LAYKNTVESI TGVISKTIST QGMLAVYNAL 370 380 390 400 410 420 SEDGKKEFEK AYSASFYPCM EILYECYEDV ASGSEIRSVV LAGRRFYEKE GLPAFPMGKI 430 440 450 460 470 480 DQTRMWKVGE RVRSTRPAGD LGPLYPFTAG VFVAMMMAQI EVLRKKGHSY SEIINESVIE 490 500 510 520 530 540 SVDSLNPFMH ARGVSFMVDN CSTTARLGSR KWAPRFDYIL TQQALVAVDS GAPINQDLIS 550 560 570 580 NFVSDPVHGA IQVCAELRPT LDISVPAAAD FVRPELRQCS N

References

[1]	Gu X., Xu Y., Wu J., Hou X., Chen Z., Zhu Y. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. G2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17796 mRNA. Translation: CAA76854.1.
PIR	T06825.
3D structure databases
HSSP	HSSP built from PDB template 1QMG based on UniProtKB Q01292.
SMR	O82043. Positions 69-578.
ModBase	Search...
Family and domain databases
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DHase_multihelical. IPR013116. IlvN. IPR016206. KetolA_reductoisomerase_pln. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000118. Ilv5_plant. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILV5_PEA

Accession

Primary (citable) accession number: O82043

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)