ID   TCMO_CICAR              Reviewed;         505 AA.
AC   O81928;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2001, sequence version 2.
DT   24-MAR-2009, entry version 53.
DE   RecName: Full=Trans-cinnamate 4-monooxygenase;
DE            EC=1.14.13.11;
DE   AltName: Full=Cinnamic acid 4-hydroxylase;
DE            Short=CA4H;
DE            Short=C4H;
DE   AltName: Full=P450C4H;
DE   AltName: Full=Cytochrome P450 73;
GN   Name=CYP73A19; Synonyms=CYP73;
OS   Cicer arietinum (Chickpea) (Garbanzo).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Cicereae;
OC   Cicer.
OX   NCBI_TaxID=3827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, AND DEVELOPMENTAL
RP   STAGE.
RC   STRAIN=cv. ILC 3279;
RX   PubMed=10773344; DOI=10.1016/S0168-9452(00)00214-4;
RA   Overkamp S., Hein F., Barz W.;
RT   "Cloning and characterization of eight cytochrome P450 cDNAs from
RT   chickpea (Cicer arietinum L.) cell suspension cultures.";
RL   Plant Sci. 155:101-108(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. ILC 3279;
RA   Overkamp S., Barz W.;
RT   "Isolation of a full length cDNA encoding trans-cinnamate 4-
RT   hydroxylase from chickpea (Cicer arietinum L.).";
RL   (er) Plant Gene Register PGR99-086.
CC   -!- FUNCTION: Controls carbon flux to pigments essential for
CC       pollination or UV protection, to numerous pytoalexins synthesized
CC       by plants when challenged by pathogens, and to lignins.
CC   -!- CATALYTIC ACTIVITY: Trans-cinnamate + NADPH + O(2) = 4-
CC       hydroxycinnamate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Phenylpropanoid metabolism; 4-coumaric acid biosynthesis;
CC       4-coumaric acid from trans-cinnamic acid: step 1/1.
CC   -!- DEVELOPMENTAL STAGE: Detectable 2 hours after elicitation and
CC       disappears after 6 hours.
CC   -!- INDUCTION: By elicitation.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AJ007449; CAA07519.2; -; mRNA.
DR   PIR; T09525; T09525.
DR   BRENDA; 1.14.13.11; 475.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0016710; F:trans-cinnamate 4-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    505       Trans-cinnamate 4-monooxygenase.
FT                                /FTId=PRO_0000052244.
FT   METAL       446    446       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   505 AA;  57889 MW;  453D1858FA996DC0 CRC64;
     MDLLLLEKTL LALFIAATIA ITISKLRGKR FKLPPGPIPV PVFGNWLQVG DDLNHRNLTD
     LAKRFGDIFL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT
     VYGTLAEMRR IMTVPFFTNK VVQQYRFGWE FEAQSVVDDV KKNPEACSSG IVLRRRLQLM
     MYNIMYRIMF DRRFESEEDP LFVKLKALNG ERSRLAQSFE YNYGDFIPIL RPFLKGYLKL
     CKEVKDRRLQ LFKDYFVDER KKLGSTKSTT NEGLKCAIDH ILDAQQKGEI NDDNVLYIVE
     NINVAAIETT LWSIEWGIAE LVNHQKIQNK VREEIDRVLG PGHQVTEPDL QKLPYLQAVI
     KETLRLRMAI PLLVPHMNLH DAKLSGFDIP AESKILVNAW WLANNPAQWK KPEEFRPERF
     LEEESHVEAN GNDFRYLPFG VGRRSCPGII LALPILGITL GRLVQNFELL PPPGQSKIDT
     AEKGGQFSLH ILKHSTIVCK PRSFN
//