O81928 (TCMO_CICAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:	CYP73A19
Synonyms:	CYP73

Organism

Cicer arietinum (Chickpea) (Garbanzo)

Taxonomic identifier

3827 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Cicereae › Cicer

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins. Ref.1
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Developmental stage	Detectable 2 hours after elicitation and disappears after 6 hours. Ref.1
Induction	By elicitation. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052244
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O81928 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 453D1858FA996DC0	FASTA	505	57,889
	10 20 30 40 50 60 MDLLLLEKTL LALFIAATIA ITISKLRGKR FKLPPGPIPV PVFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKRFGDIFL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGTLAEMRR IMTVPFFTNK VVQQYRFGWE FEAQSVVDDV KKNPEACSSG IVLRRRLQLM 190 200 210 220 230 240 MYNIMYRIMF DRRFESEEDP LFVKLKALNG ERSRLAQSFE YNYGDFIPIL RPFLKGYLKL 250 260 270 280 290 300 CKEVKDRRLQ LFKDYFVDER KKLGSTKSTT NEGLKCAIDH ILDAQQKGEI NDDNVLYIVE 310 320 330 340 350 360 NINVAAIETT LWSIEWGIAE LVNHQKIQNK VREEIDRVLG PGHQVTEPDL QKLPYLQAVI 370 380 390 400 410 420 KETLRLRMAI PLLVPHMNLH DAKLSGFDIP AESKILVNAW WLANNPAQWK KPEEFRPERF 430 440 450 460 470 480 LEEESHVEAN GNDFRYLPFG VGRRSCPGII LALPILGITL GRLVQNFELL PPPGQSKIDT 490 500 AEKGGQFSLH ILKHSTIVCK PRSFN

References

[1]	"Cloning and characterization of eight cytochrome P450 cDNAs from chickpea (Cicer arietinum L.) cell suspension cultures." Overkamp S., Hein F., Barz W. Plant Sci. 155:101-108(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, DEVELOPMENTAL STAGE. Strain: cv. ILC 3279.
[2]	"Isolation of a full length cDNA encoding trans-cinnamate 4-hydroxylase from chickpea (Cicer arietinum L.)." Overkamp S., Barz W. Plant Gene Register PGR99-086 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. ILC 3279.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ007449 mRNA. Translation: CAA07519.2.
PIR	T09525.
3D structure databases
ProteinModelPortal	O81928.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00825; UER00789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_CICAR

Accession

Primary (citable) accession number: O81928

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 11, 2001
Last modified:	March 6, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program