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Protein

Inositol-tetrakisphosphate 1-kinase 2

Gene

ITPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 to form InsP5 (PubMed:17698066). This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca2+-activated Cl- channels, while Ins(1,3,4,5,6)P5 is not (By similarity). Also phosphorylates Ins(1,3,4)P3 or a racemic mixture of Ins(1,4,6)P3 and Ins(3,4,6)P3 to form InsP4 (PubMed:17698066). Ins(1,3,4,6)P4 is an essential molecule in the hexakisphosphate (InsP6) pathway (By similarity). Plays a role in seed coat development and lipid polyester barrier formation (PubMed:23595027).By similarity2 Publications

Catalytic activityi

ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.1 Publication
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei901D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei1321D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei167ATPBy similarity1
Binding sitei217ATPBy similarity1
Binding sitei2281D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei2601D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei275ATPBy similarity1
Metal bindingi340Magnesium 1By similarity1
Metal bindingi355Magnesium 1By similarity1
Metal bindingi355Magnesium 2By similarity1
Metal bindingi357Magnesium 2By similarity1
Binding sitei3571D-myo-inositol 1,3,4-trisphosphateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 260ATPBy similarityAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • inositol-1,3,4-trisphosphate 5-kinase activity Source: UniProtKB
  • inositol-1,3,4-trisphosphate 6-kinase activity Source: UniProtKB
  • inositol tetrakisphosphate 1-kinase activity Source: UniProtKB
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • inositol trisphosphate metabolic process Source: InterPro
  • seed coat development Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G33770-MONOMER
BRENDAi2.7.1.159 399
ReactomeiR-ATH-1855167 Synthesis of pyrophosphates in the cytosol
R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol
R-ATH-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-tetrakisphosphate 1-kinase 2 (EC:2.7.1.1341 Publication)
Alternative name(s):
Inositol 1,3,4-trisphosphate 5/6-kinase 2 (EC:2.7.1.1591 Publication)
Short name:
AtItpk-2
Short name:
Inositol-triphosphate 5/6-kinase 2
Short name:
Ins(1,3,4)P(3) 5/6-kinase 2
Gene namesi
Name:ITPK2
Ordered Locus Names:At4g33770
ORF Names:T16L1.260
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G33770
TAIRilocus:2134253 AT4G33770

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Distorted seed coat with reduced mucilage content and decreased suberin and cutin composition. Crumpled columellas.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208411 – 391Inositol-tetrakisphosphate 1-kinase 2Add BLAST391

Proteomic databases

PaxDbiO81893
PRIDEiO81893

Expressioni

Tissue specificityi

Expressed in seedling roots, cotyledons, rosette leaves, cauline leaves, stems, flowers, siliques and seeds.2 Publications

Developmental stagei

Expressed in the seed coat of developing seeds from 2 to 6 days after fertilization.1 Publication

Gene expression databases

ExpressionAtlasiO81893 differential
GenevisibleiO81893 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi14801, 1 interactor
IntActiO81893, 1 interactor
STRINGi3702.AT4G33770.1

Structurei

3D structure databases

ProteinModelPortaliO81893
SMRiO81893
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 384ATP-graspAdd BLAST207

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi50 – 56Poly-Glu7

Sequence similaritiesi

Belongs to the ITPK1 family.Curated

Phylogenomic databases

eggNOGiENOG410IHA6 Eukaryota
ENOG4110KIK LUCA
HOGENOMiHOG000220790
InParanoidiO81893
KOiK00913
OMAiRFEGENM
OrthoDBiEOG09360GWF
PhylomeDBiO81893

Family and domain databases

InterProiView protein in InterPro
IPR008656 Inositol_tetrakis-P_1-kinase
PANTHERiPTHR14217 PTHR14217, 1 hit
PfamiView protein in Pfam
PF05770 Ins134_P3_kin, 1 hit
PIRSFiPIRSF038186 ITPK, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O81893-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGTLASGEI ETARLNRNLG ITSNLGVSCG GFEDFAMRFE GENMVPYKGE
60 70 80 90 100
EQEEEEDQVV VNETTPFQFQ QPLFLQQQQK LVVGYALTSK KKKSFLQPKL
110 120 130 140 150
ELLARRKGIF FVAIDLNRPL SEQGPFDVVL HKLLGKEWEE VIEDYQQKHP
160 170 180 190 200
EVTVLDPPGS IQRIYNRQSM LQGMADLKLS DCSGSLFVPK QMVVLKDSAA
210 220 230 240 250
SADAVVEAGL KFPLVAKPLW IDGTAKSHQL YLAYDRRSLA ELDPPLVLQE
260 270 280 290 300
FVNHGGVMFK VFVVGDVIKV MRRFSLPNVS NCEKAKVDGV FQFPRVSSAA
310 320 330 340 350
ASADNADLDP RVAELPPKPF LEALVKELRS LLGLRLFNID MIREHGSKNV
360 370 380 390
FYVIDINYFP GYGKLPDYEQ VFVDFFQNLA QVKYKKRQHC K
Length:391
Mass (Da):44,172
Last modified:June 26, 2007 - v3
Checksum:i56BFA59FCCF0FC1A
GO

Sequence cautioni

The sequence CAA20590 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80094 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL031394 Genomic DNA Translation: CAA20590.1 Sequence problems.
AL161584 Genomic DNA Translation: CAB80094.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86275.1
BT029235 mRNA Translation: ABJ98567.1
PIRiT04994
RefSeqiNP_195103.3, NM_119535.5 [O81893-1]
UniGeneiAt.31554

Genome annotation databases

EnsemblPlantsiAT4G33770.1; AT4G33770.1; AT4G33770 [O81893-1]
GeneIDi829519
GrameneiAT4G33770.1; AT4G33770.1; AT4G33770 [O81893-1]
KEGGiath:AT4G33770

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiITPK2_ARATH
AccessioniPrimary (citable) accession number: O81893
Secondary accession number(s): Q058I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 26, 2007
Last modified: May 23, 2018
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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