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Protein

Mitochondrial uncoupling protein 1

Gene

PUMP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration.6 Publications

GO - Molecular functioni

GO - Biological processi

  • mitochondrial transport Source: InterPro
  • photorespiration Source: TAIR
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Stress response, Transport

Enzyme and pathway databases

ReactomeiR-ATH-167826. The fatty acid cycling model.
R-ATH-167827. The proton buffering model.

Protein family/group databases

TCDBi2.A.29.3.3. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial uncoupling protein 1
Short name:
AtPUMP1
Gene namesi
Name:PUMP1
Synonyms:UCP1
Ordered Locus Names:At3g54110
ORF Names:F24B22.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei71 – 9121Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei118 – 13821Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei177 – 19721Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei218 – 23821Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei269 – 28921Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Slight reduction of shoot mass.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281C → A: Reduces proton transport activity 3-fold. 1 Publication
Mutagenesisi83 – 831H → L: Reduces proton transport activity 3-fold. 1 Publication
Mutagenesisi147 – 1471K → H: Reduces proton transport activity 3-fold. 1 Publication
Mutagenesisi155 – 1551R → L: Slightly reduces proton transport activity. 1 Publication
Mutagenesisi269 – 2691Y → F: Reduces proton transport activity 3-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Mitochondrial uncoupling protein 1PRO_0000420255Add
BLAST

Proteomic databases

PaxDbiO81845.
PRIDEiO81845.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Inductioni

By cold stress, high light and chloramphenicol.3 Publications

Gene expression databases

GenevisibleiO81845. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G54110.1.

Structurei

3D structure databases

ProteinModelPortaliO81845.
SMRiO81845. Positions 15-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 10294Solcar 1Add
BLAST
Repeati112 – 20392Solcar 2Add
BLAST
Repeati212 – 29685Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
InParanoidiO81845.
KOiK15103.
OMAiLLKAHLM.
PhylomeDBiO81845.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAAGKSDLS LPKTFACSAF AACVGEVCTI PLDTAKVRLQ LQKSALAGDV
60 70 80 90 100
TLPKYRGLLG TVGTIAREEG LRSLWKGVVP GLHRQCLFGG LRIGMYEPVK
110 120 130 140 150
NLYVGKDFVG DVPLSKKILA GLTTGALGIM VANPTDLVKV RLQAEGKLAA
160 170 180 190 200
GAPRRYSGAL NAYSTIVRQE GVRALWTGLG PNVARNAIIN AAELASYDQV
210 220 230 240 250
KETILKIPGF TDNVVTHILS GLGAGFFAVC IGSPVDVVKS RMMGDSGAYK
260 270 280 290 300
GTIDCFVKTL KSDGPMAFYK GFIPNFGRLG SWNVIMFLTL EQAKKYVREL

DASKRN
Length:306
Mass (Da):32,662
Last modified:November 1, 1998 - v1
Checksum:iACCCB46F42442142
GO

Sequence cautioni

The sequence BAD95028.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781G → V in CAA11757 (PubMed:9662458).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223983 mRNA. Translation: CAA11757.1.
AJ001264 mRNA. Translation: CAA04638.1.
Y18291 Genomic DNA. Translation: CAA77109.1.
AF146226 mRNA. Translation: AAF66705.1.
AL132957 Genomic DNA. Translation: CAB70985.1.
CP002686 Genomic DNA. Translation: AEE79189.1.
AY056272 mRNA. Translation: AAL07121.1.
AY091185 mRNA. Translation: AAM14124.1.
AK221112 mRNA. Translation: BAD95028.1. Different initiation.
PIRiT47570.
T52024.
RefSeqiNP_190979.1. NM_115271.4.
UniGeneiAt.25009.
At.68442.

Genome annotation databases

EnsemblPlantsiAT3G54110.1; AT3G54110.1; AT3G54110.
GeneIDi824578.
GrameneiAT3G54110.1; AT3G54110.1; AT3G54110.
KEGGiath:AT3G54110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223983 mRNA. Translation: CAA11757.1.
AJ001264 mRNA. Translation: CAA04638.1.
Y18291 Genomic DNA. Translation: CAA77109.1.
AF146226 mRNA. Translation: AAF66705.1.
AL132957 Genomic DNA. Translation: CAB70985.1.
CP002686 Genomic DNA. Translation: AEE79189.1.
AY056272 mRNA. Translation: AAL07121.1.
AY091185 mRNA. Translation: AAM14124.1.
AK221112 mRNA. Translation: BAD95028.1. Different initiation.
PIRiT47570.
T52024.
RefSeqiNP_190979.1. NM_115271.4.
UniGeneiAt.25009.
At.68442.

3D structure databases

ProteinModelPortaliO81845.
SMRiO81845. Positions 15-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G54110.1.

Protein family/group databases

TCDBi2.A.29.3.3. the mitochondrial carrier (mc) family.

Proteomic databases

PaxDbiO81845.
PRIDEiO81845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54110.1; AT3G54110.1; AT3G54110.
GeneIDi824578.
GrameneiAT3G54110.1; AT3G54110.1; AT3G54110.
KEGGiath:AT3G54110.

Organism-specific databases

TAIRiAT3G54110.

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
InParanoidiO81845.
KOiK15103.
OMAiLLKAHLM.
PhylomeDBiO81845.

Enzyme and pathway databases

ReactomeiR-ATH-167826. The fatty acid cycling model.
R-ATH-167827. The proton buffering model.

Miscellaneous databases

PROiO81845.

Gene expression databases

GenevisibleiO81845. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "AtPUMP: an Arabidopsis gene encoding a plant uncoupling mitochondrial protein."
    Maia I.G., Benedetti C.E., Leite A., Turcinelli S.R., Vercesi A.E., Arruda P.
    FEBS Lett. 429:403-406(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY COLD.
    Strain: cv. Columbia.
  2. "Arabidopsis thaliana mitochondrial uncoupling protein."
    Laloi M., Klein M., Mueller-Roeber B., Willmitzer L.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia and cv. Landsberg erecta.
  3. "Expression of a plant mitochondrial uncoupling protein in yeast."
    Dombrowski H.D., Cousins S.K., Harris M.A., Hanson M.R.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 123-306.
    Strain: cv. Columbia.
  8. "Functional reconstitution of Arabidopsis thaliana plant uncoupling mitochondrial protein (AtPUMP1) expressed in Escherichia coli."
    Borecky J., Maia I.G., Costa A.D., Jezek P., Chaimovich H., de Andrade P.B., Vercesi A.E., Arruda P.
    FEBS Lett. 505:240-244(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress."
    Brandalise M., Maia I.G., Borecky J., Vercesi A.E., Arruda P.
    J. Bioenerg. Biomembr. 35:203-209(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Stress-induced co-expression of alternative respiratory chain components in Arabidopsis thaliana."
    Clifton R., Lister R., Parker K.L., Sappl P.G., Elhafez D., Millar A.H., Day D.A., Whelan J.
    Plant Mol. Biol. 58:193-212(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CHLORAMPHENICOL.
  11. "The plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots."
    Borecky J., Nogueira F.T., de Oliveira K.A., Maia I.G., Vercesi A.E., Arruda P.
    J. Exp. Bot. 57:849-864(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  12. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  13. "Mutational analysis of Arabidopsis thaliana plant uncoupling mitochondrial protein."
    Favaro R.D., Borecky J., Colombi D., Vercesi A.E., Maia I.G.
    Biochim. Biophys. Acta 1767:1412-1417(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF CYS-28; HIS-83; LYS-147; ARG-155 AND TYR-269.
  14. "Differential gene expression profiles of the mitochondrial respiratory components in illuminated Arabidopsis leaves."
    Yoshida K., Noguchi K.
    Plant Cell Physiol. 50:1449-1462(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HIGH LIGHT.
  15. "Genetically modified Arabidopsis thaliana cells reveal the involvement of the mitochondrial fatty acid composition in membrane basal and uncoupling protein-mediated proton leaks."
    Hourton-Cabassa C., Matos A.R., Arrabaca J., Demandre C., Zachowski A., Moreau F.
    Plant Cell Physiol. 50:2084-2091(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "An Arabidopsis mitochondrial uncoupling protein confers tolerance to drought and salt stress in transgenic tobacco plants."
    Begcy K., Mariano E.D., Mattiello L., Nunes A.V., Mazzafera P., Maia I.G., Menossi M.
    PLoS ONE 6:E23776-E23776(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPUMP1_ARATH
AccessioniPrimary (citable) accession number: O81845
Secondary accession number(s): O65623, Q56Z57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1998
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.