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Protein

Mitochondrial uncoupling protein 1

Gene

PUMP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration.6 Publications

GO - Molecular functioni

  • oxidative phosphorylation uncoupler activity Source: TAIR

GO - Biological processi

  • mitochondrial transport Source: InterPro
  • photorespiration Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Stress response, Transport

Enzyme and pathway databases

ReactomeiR-ATH-167826. The fatty acid cycling model.
R-ATH-167827. The proton buffering model.

Protein family/group databases

TCDBi2.A.29.3.3. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial uncoupling protein 1
Short name:
AtPUMP1
Gene namesi
Name:PUMP1
Synonyms:UCP1
Ordered Locus Names:At3g54110
ORF Names:F24B22.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54110.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei71 – 91Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei118 – 138Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei177 – 197Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei218 – 238Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei269 – 289Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • chloroplast Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Slight reduction of shoot mass.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28C → A: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi83H → L: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi147K → H: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi155R → L: Slightly reduces proton transport activity. 1 Publication1
Mutagenesisi269Y → F: Reduces proton transport activity 3-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004202551 – 306Mitochondrial uncoupling protein 1Add BLAST306

Proteomic databases

PaxDbiO81845.
PRIDEiO81845.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Inductioni

By cold stress, high light and chloramphenicol.3 Publications

Gene expression databases

GenevisibleiO81845. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G54110.1.

Structurei

3D structure databases

ProteinModelPortaliO81845.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 102Solcar 1Add BLAST94
Repeati112 – 203Solcar 2Add BLAST92
Repeati212 – 296Solcar 3Add BLAST85

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
InParanoidiO81845.
KOiK15103.
OMAiLLKAHLM.
OrthoDBiEOG09360HWE.
PhylomeDBiO81845.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAAGKSDLS LPKTFACSAF AACVGEVCTI PLDTAKVRLQ LQKSALAGDV
60 70 80 90 100
TLPKYRGLLG TVGTIAREEG LRSLWKGVVP GLHRQCLFGG LRIGMYEPVK
110 120 130 140 150
NLYVGKDFVG DVPLSKKILA GLTTGALGIM VANPTDLVKV RLQAEGKLAA
160 170 180 190 200
GAPRRYSGAL NAYSTIVRQE GVRALWTGLG PNVARNAIIN AAELASYDQV
210 220 230 240 250
KETILKIPGF TDNVVTHILS GLGAGFFAVC IGSPVDVVKS RMMGDSGAYK
260 270 280 290 300
GTIDCFVKTL KSDGPMAFYK GFIPNFGRLG SWNVIMFLTL EQAKKYVREL

DASKRN
Length:306
Mass (Da):32,662
Last modified:November 1, 1998 - v1
Checksum:iACCCB46F42442142
GO

Sequence cautioni

The sequence BAD95028 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178G → V in CAA11757 (PubMed:9662458).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223983 mRNA. Translation: CAA11757.1.
AJ001264 mRNA. Translation: CAA04638.1.
Y18291 Genomic DNA. Translation: CAA77109.1.
AF146226 mRNA. Translation: AAF66705.1.
AL132957 Genomic DNA. Translation: CAB70985.1.
CP002686 Genomic DNA. Translation: AEE79189.1.
AY056272 mRNA. Translation: AAL07121.1.
AY091185 mRNA. Translation: AAM14124.1.
AK221112 mRNA. Translation: BAD95028.1. Different initiation.
PIRiT47570.
T52024.
RefSeqiNP_190979.1. NM_115271.5.
UniGeneiAt.25009.
At.68442.

Genome annotation databases

EnsemblPlantsiAT3G54110.1; AT3G54110.1; AT3G54110.
GeneIDi824578.
GrameneiAT3G54110.1; AT3G54110.1; AT3G54110.
KEGGiath:AT3G54110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223983 mRNA. Translation: CAA11757.1.
AJ001264 mRNA. Translation: CAA04638.1.
Y18291 Genomic DNA. Translation: CAA77109.1.
AF146226 mRNA. Translation: AAF66705.1.
AL132957 Genomic DNA. Translation: CAB70985.1.
CP002686 Genomic DNA. Translation: AEE79189.1.
AY056272 mRNA. Translation: AAL07121.1.
AY091185 mRNA. Translation: AAM14124.1.
AK221112 mRNA. Translation: BAD95028.1. Different initiation.
PIRiT47570.
T52024.
RefSeqiNP_190979.1. NM_115271.5.
UniGeneiAt.25009.
At.68442.

3D structure databases

ProteinModelPortaliO81845.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G54110.1.

Protein family/group databases

TCDBi2.A.29.3.3. the mitochondrial carrier (mc) family.

Proteomic databases

PaxDbiO81845.
PRIDEiO81845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54110.1; AT3G54110.1; AT3G54110.
GeneIDi824578.
GrameneiAT3G54110.1; AT3G54110.1; AT3G54110.
KEGGiath:AT3G54110.

Organism-specific databases

TAIRiAT3G54110.

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
InParanoidiO81845.
KOiK15103.
OMAiLLKAHLM.
OrthoDBiEOG09360HWE.
PhylomeDBiO81845.

Enzyme and pathway databases

ReactomeiR-ATH-167826. The fatty acid cycling model.
R-ATH-167827. The proton buffering model.

Miscellaneous databases

PROiO81845.

Gene expression databases

GenevisibleiO81845. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUMP1_ARATH
AccessioniPrimary (citable) accession number: O81845
Secondary accession number(s): O65623, Q56Z57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.