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Protein

Mitochondrial uncoupling protein 1

Gene

PUMP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration.6 Publications

Miscellaneous

Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt.1 Publication

GO - Molecular functioni

  • oxidative phosphorylation uncoupler activity Source: TAIR

GO - Biological processi

  • mitochondrial transmembrane transport Source: GO_Central
  • mitochondrial transport Source: GO_Central
  • photorespiration Source: TAIR

Keywordsi

Biological processStress response, Transport

Enzyme and pathway databases

ReactomeiR-ATH-167826 The fatty acid cycling model
R-ATH-167827 The proton buffering model

Protein family/group databases

TCDBi2.A.29.3.3 the mitochondrial carrier (mc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial uncoupling protein 1
Short name:
AtPUMP1
Gene namesi
Name:PUMP1
Synonyms:UCP1
Ordered Locus Names:At3g54110
ORF Names:F24B22.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54110
TAIRilocus:2080300 AT3G54110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei71 – 91Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei118 – 138Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei177 – 197Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei218 – 238Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei269 – 289Helical; Name=6Sequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Slight reduction of shoot mass.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28C → A: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi83H → L: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi147K → H: Reduces proton transport activity 3-fold. 1 Publication1
Mutagenesisi155R → L: Slightly reduces proton transport activity. 1 Publication1
Mutagenesisi269Y → F: Reduces proton transport activity 3-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004202551 – 306Mitochondrial uncoupling protein 1Add BLAST306

Proteomic databases

PaxDbiO81845
PRIDEiO81845

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Inductioni

By cold stress, high light and chloramphenicol.3 Publications

Gene expression databases

ExpressionAtlasiO81845 baseline and differential
GenevisibleiO81845 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G54110.1

Structurei

3D structure databases

ProteinModelPortaliO81845
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 102Solcar 1Add BLAST94
Repeati112 – 203Solcar 2Add BLAST92
Repeati212 – 296Solcar 3Add BLAST85

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0753 Eukaryota
ENOG410XRV1 LUCA
HOGENOMiHOG000165140
InParanoidiO81845
KOiK15103
OMAiSEHAGIG
OrthoDBiEOG09360HWE
PhylomeDBiO81845

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR002030 Mit_uncoupling_UCP-like
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00784 MTUNCOUPLING
SUPFAMiSSF103506 SSF103506, 1 hit
PROSITEiView protein in PROSITE
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

O81845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAAGKSDLS LPKTFACSAF AACVGEVCTI PLDTAKVRLQ LQKSALAGDV
60 70 80 90 100
TLPKYRGLLG TVGTIAREEG LRSLWKGVVP GLHRQCLFGG LRIGMYEPVK
110 120 130 140 150
NLYVGKDFVG DVPLSKKILA GLTTGALGIM VANPTDLVKV RLQAEGKLAA
160 170 180 190 200
GAPRRYSGAL NAYSTIVRQE GVRALWTGLG PNVARNAIIN AAELASYDQV
210 220 230 240 250
KETILKIPGF TDNVVTHILS GLGAGFFAVC IGSPVDVVKS RMMGDSGAYK
260 270 280 290 300
GTIDCFVKTL KSDGPMAFYK GFIPNFGRLG SWNVIMFLTL EQAKKYVREL

DASKRN
Length:306
Mass (Da):32,662
Last modified:November 1, 1998 - v1
Checksum:iACCCB46F42442142
GO

Sequence cautioni

The sequence BAD95028 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178G → V in CAA11757 (PubMed:9662458).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223983 mRNA Translation: CAA11757.1
AJ001264 mRNA Translation: CAA04638.1
Y18291 Genomic DNA Translation: CAA77109.1
AF146226 mRNA Translation: AAF66705.1
AL132957 Genomic DNA Translation: CAB70985.1
CP002686 Genomic DNA Translation: AEE79189.1
AY056272 mRNA Translation: AAL07121.1
AY091185 mRNA Translation: AAM14124.1
AK221112 mRNA Translation: BAD95028.1 Different initiation.
PIRiT47570
T52024
RefSeqiNP_190979.1, NM_115271.5
UniGeneiAt.25009
At.68442

Genome annotation databases

EnsemblPlantsiAT3G54110.1; AT3G54110.1; AT3G54110
GeneIDi824578
GrameneiAT3G54110.1; AT3G54110.1; AT3G54110
KEGGiath:AT3G54110

Similar proteinsi

Entry informationi

Entry nameiPUMP1_ARATH
AccessioniPrimary (citable) accession number: O81845
Secondary accession number(s): O65623, Q56Z57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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