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Protein

Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial

Gene

IDH3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Performs an essential role in the oxidative function of the citric acid cycle.By similarity

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateBy similarity1
Binding sitei148SubstrateBy similarity1
Sitei155Critical for catalysisBy similarity1
Sitei202Critical for catalysisBy similarity1
Metal bindingi235Magnesium or manganeseBy similarity1
Binding sitei235SubstrateBy similarity1

GO - Molecular functioni

  • isocitrate dehydrogenase (NAD+) activity Source: TAIR
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

  • isocitrate metabolic process Source: TAIR
  • tricarboxylic acid cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BRENDAi1.1.1.41. 399.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial (EC:1.1.1.41)
Alternative name(s):
IDH-III
Isocitric dehydrogenase 3
NAD(+)-specific ICDH 3
Gene namesi
Name:IDH3
Ordered Locus Names:At4g35650
ORF Names:F8D20.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G35650.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000027128927 – 368Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrialAdd BLAST342

Proteomic databases

PaxDbiO81796.
PRIDEiO81796.

PTM databases

iPTMnetiO81796.

Expressioni

Tissue specificityi

Mainly expressed at a low level in pollen.1 Publication

Gene expression databases

GenevisibleiO81796. AT.

Interactioni

Subunit structurei

Heterooligomer of catalytic and regulatory subunits. Interacts with 14-3-3-like proteins GRF1 GRF3 and GRF8 (PubMed:22104211).1 Publication

Protein-protein interaction databases

BioGridi14999. 3 interactors.
MINTiMINT-8061753.
STRINGi3702.AT4G35650.1.

Structurei

3D structure databases

ProteinModelPortaliO81796.
SMRiO81796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0784. Eukaryota.
COG0473. LUCA.
HOGENOMiHOG000021113.
InParanoidiO81796.
KOiK00030.
OMAiASLVNCI.
OrthoDBiEOG09360FRN.
PhylomeDBiO81796.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O81796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRSVSIFN RLLANPPSPF TSLSRSITYM PRPGDGAPRT VTLIPGDGIG
60 70 80 90 100
PLVTGAVEQV MEAMHAPVHF ERYEVLGNMR KVPEEVIESV KRNKVCLKGG
110 120 130 140 150
LATPVGGGVS SLNMQLRKEL DIFASLVNCI NVPGLVTRHE NVDIVVIREN
160 170 180 190 200
TEGEYSGLEH EVVPGVVESL KVITKFCSER IARYAFEYAY LNNRKKVTAV
210 220 230 240 250
HKANIMKLAD GLFLESCREV AKHYSGITYN EIIVDNCCMQ LVAKPEQFDV
260 270 280 290 300
MVTPNLYGNL IANTAAGIAG GTGVMPGGNV GAEHAIFEQG ASAGNVGNDK
310 320 330 340 350
MVEQKKANPV ALLLSSAMML RHLRFPTFAD RLETAVKQVI KEGKYRTKDL
360
GGDCTTQEVV DAVIAALE
Length:368
Mass (Da):39,957
Last modified:November 1, 1998 - v1
Checksum:i28EDD697B921650D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL031135 Genomic DNA. Translation: CAA20035.1.
AL161587 Genomic DNA. Translation: CAB80281.1.
CP002687 Genomic DNA. Translation: AEE86544.1.
BT003922 mRNA. Translation: AAO41969.1.
BT006081 mRNA. Translation: AAP04066.1.
PIRiT04670.
RefSeqiNP_195290.1. NM_119730.3.
UniGeneiAt.31403.

Genome annotation databases

EnsemblPlantsiAT4G35650.1; AT4G35650.1; AT4G35650.
GeneIDi829717.
GrameneiAT4G35650.1; AT4G35650.1; AT4G35650.
KEGGiath:AT4G35650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL031135 Genomic DNA. Translation: CAA20035.1.
AL161587 Genomic DNA. Translation: CAB80281.1.
CP002687 Genomic DNA. Translation: AEE86544.1.
BT003922 mRNA. Translation: AAO41969.1.
BT006081 mRNA. Translation: AAP04066.1.
PIRiT04670.
RefSeqiNP_195290.1. NM_119730.3.
UniGeneiAt.31403.

3D structure databases

ProteinModelPortaliO81796.
SMRiO81796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14999. 3 interactors.
MINTiMINT-8061753.
STRINGi3702.AT4G35650.1.

PTM databases

iPTMnetiO81796.

Proteomic databases

PaxDbiO81796.
PRIDEiO81796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G35650.1; AT4G35650.1; AT4G35650.
GeneIDi829717.
GrameneiAT4G35650.1; AT4G35650.1; AT4G35650.
KEGGiath:AT4G35650.

Organism-specific databases

TAIRiAT4G35650.

Phylogenomic databases

eggNOGiKOG0784. Eukaryota.
COG0473. LUCA.
HOGENOMiHOG000021113.
InParanoidiO81796.
KOiK00030.
OMAiASLVNCI.
OrthoDBiEOG09360FRN.
PhylomeDBiO81796.

Enzyme and pathway databases

BRENDAi1.1.1.41. 399.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiO81796.

Gene expression databases

GenevisibleiO81796. AT.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIDH3_ARATH
AccessioniPrimary (citable) accession number: O81796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.