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O81770 (MGDG1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monogalactosyldiacylglycerol synthase 1, chloroplastic

Short name=AtMGD1
EC=2.4.1.46
Alternative name(s):
MGDG synthase type A
Gene names
Name:MGD1
Synonyms:MGDA
Ordered Locus Names:At4g31780
ORF Names:F28M20.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the synthesis of the major structural component of photosynthetic membranes. Required for proper thylakoid membrane biogenesis. Does not discriminate between prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the prokaryotic one. Ref.1 Ref.2 Ref.6 Ref.8

Catalytic activity

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast inner membrane Probable Ref.2 Ref.6.

Tissue specificity

Expressed in roots, stems, leaves, flowers, siliques and seeds. Ref.2 Ref.6 Ref.7

Developmental stage

Highly expressed throughout whole developmental stages. Transient increase in embryos at the globular stage. Ref.2 Ref.8

Induction

Induced by illumination and cytokinin treatment in etiolated seedlings. Not induced by phosphate deprivation. Ref.2 Ref.7

Disruption phenotype

Plants show defects in chloroplast biogenesis and a dwarf and albino phenotype. The amount of monogalactosyldiacylglycerol was reduced by 98% in the mutant leaves, indicating that MGD2/MGD3 cannot compensate for loss of MGD1 function. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 28 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O81770-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O81770-2)

The sequence of this isoform differs from the canonical sequence as follows:
     456-504: EAGNVPYVVE...IMSQNALRLA → VSRECTVRGG...DNVTECIEAG
     505-533: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 106106Chloroplast Potential
Chain107 – 533427Monogalactosyldiacylglycerol synthase 1, chloroplastic
PRO_0000349421

Natural variations

Alternative sequence456 – 50449EAGNV…ALRLA → VSRECTVRGGKRMWEILKIT ERDIEDCSGLVWTGIERVGD NVTECIEAG in isoform 2.
VSP_035387
Alternative sequence505 – 53329Missing in isoform 2.
VSP_035388

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E581E67317CB9CC8

FASTA53358,538
        10         20         30         40         50         60 
MQNPSTVTQE SAAPVFDFFP RLRGLTSRNR SPCSNSDGYA LSSSNALYFN GFRTLPSRRM 

        70         80         90        100        110        120 
GKTLASLSFN TKSSAGSSLR RFISDFNSFI RFHCDKVVPE SFASVGGVGL SSDENGIREN 

       130        140        150        160        170        180 
GTGGVLGEEG LPLNGVEADR PKKVLILMSD TGGGHRASAE AIRAAFNQEF GDEYQVFITD 

       190        200        210        220        230        240 
LWTDHTPWPF NQLPRSYNFL VKHGTLWKMT YYGTSPRIVH QSNFAATSTF IAREIAQGLM 

       250        260        270        280        290        300 
KYQPDIIISV HPLMQHVPLR VLRSKGLLKK IVFTTVITDL STCHPTWFHK LVTRCYCPST 

       310        320        330        340        350        360 
EVAKRAQKAG LETSQIKVYG LPVRPSFVKP VRPKVELRRE LGMDENLPAV LLMGGGEGMG 

       370        380        390        400        410        420 
PIEATARALA DALYDKNLGE AVGQVLIICG RNKKLQSKLS SLDWKIPVQV KGFITKMEEC 

       430        440        450        460        470        480 
MGACDCIITK AGPGTIAEAM IRGLPIILNG YIAGQEAGNV PYVVENGCGK FSKSPKEISK 

       490        500        510        520        530 
IVADWFGPAS KELEIMSQNA LRLAKPEAVF KIVHDMHELV RKKNSLPQLS CTA 

« Hide

Isoform 2 [UniParc].

Checksum: 51ED4D5A942E9ACA
Show »

FASTA50455,437

References

« Hide 'large scale' references
[1]"Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant."
Jarvis P., Doermann P., Peto C.A., Lutes J., Benning C., Chory J.
Proc. Natl. Acad. Sci. U.S.A. 97:8175-8179(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[2]"Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"The multigenic family of monogalactosyl diacylglycerol synthases."
Marechal E., Awai K., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
Biochem. Soc. Trans. 28:732-738(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, CHARACTERIZATION, SUBCELLULAR LOCATION, NOMENCLATURE.
[7]"Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
Kobayashi K., Awai K., Takamiya K., Ohta H.
Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT AND CYTOKININ.
[8]"Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis."
Kobayashi K., Kondo M., Fukuda H., Nishimura M., Ohta H.
Proc. Natl. Acad. Sci. U.S.A. 104:17216-17221(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF241797 mRNA. Translation: AAF65066.1.
AB047399 mRNA. Translation: BAB12042.1.
AL031004 Genomic DNA. Translation: CAA19745.1.
AL161579 Genomic DNA. Translation: CAB79896.1.
CP002687 Genomic DNA. Translation: AEE85956.1.
CP002687 Genomic DNA. Translation: AEE85957.1.
AY092965 mRNA. Translation: AAM12964.1.
BT008890 mRNA. Translation: AAP68329.1.
PIRT05092.
RefSeqNP_194906.1. NM_119327.2. [O81770-1]
NP_849482.1. NM_179151.1. [O81770-2]
UniGeneAt.1647.

3D structure databases

ProteinModelPortalO81770.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT28. Glycosyltransferase Family 28.

Proteomic databases

PaxDbO81770.
PRIDEO81770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G31780.2; AT4G31780.2; AT4G31780. [O81770-1]
GeneID829306.
KEGGath:AT4G31780.

Organism-specific databases

TAIRAT4G31780.

Phylogenomic databases

eggNOGCOG0707.
HOGENOMHOG000239591.
InParanoidQ9MU68.
KOK03715.
OMAIATVMTD.
PhylomeDBO81770.

Enzyme and pathway databases

BioCycARA:GQT-2230-MONOMER.
MetaCyc:AT4G31780-MONOMER.

Gene expression databases

GenevestigatorO81770.

Family and domain databases

InterProIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGDG1_ARATH
AccessionPrimary (citable) accession number: O81770
Secondary accession number(s): Q3E9T1, Q9MU68
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names