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O81770

- MGDG1_ARATH

UniProt

O81770 - MGDG1_ARATH

Protein

Monogalactosyldiacylglycerol synthase 1, chloroplastic

Gene

MGD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the major structural component of photosynthetic membranes. Required for proper thylakoid membrane biogenesis. Does not discriminate between prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the prokaryotic one.4 Publications

    Catalytic activityi

    UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

    pH dependencei

    Optimum pH is 7.5.

    GO - Molecular functioni

    1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: TAIR
    2. carbohydrate binding Source: InterPro
    3. UDP-galactosyltransferase activity Source: TAIR
    4. UDP-glycosyltransferase activity Source: TAIR

    GO - Biological processi

    1. glycolipid biosynthetic process Source: TAIR
    2. lipid glycosylation Source: InterPro
    3. thylakoid membrane organization Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:GQT-2230-MONOMER.
    MetaCyc:AT4G31780-MONOMER.

    Protein family/group databases

    CAZyiGT28. Glycosyltransferase Family 28.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monogalactosyldiacylglycerol synthase 1, chloroplastic (EC:2.4.1.46)
    Short name:
    AtMGD1
    Alternative name(s):
    MGDG synthase type A
    Gene namesi
    Name:MGD1
    Synonyms:MGDA
    Ordered Locus Names:At4g31780
    ORF Names:F28M20.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G31780.

    Subcellular locationi

    Plastidchloroplast inner membrane 2 Publications

    GO - Cellular componenti

    1. chloroplast envelope Source: TAIR
    2. chloroplast inner membrane Source: TAIR
    3. plastid Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Plants show defects in chloroplast biogenesis and a dwarf and albino phenotype. The amount of monogalactosyldiacylglycerol was reduced by 98% in the mutant leaves, indicating that MGD2/MGD3 cannot compensate for loss of MGD1 function.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 106106ChloroplastSequence AnalysisAdd
    BLAST
    Chaini107 – 533427Monogalactosyldiacylglycerol synthase 1, chloroplasticPRO_0000349421Add
    BLAST

    Proteomic databases

    PaxDbiO81770.
    PRIDEiO81770.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers, siliques and seeds.3 Publications

    Developmental stagei

    Highly expressed throughout whole developmental stages. Transient increase in embryos at the globular stage.2 Publications

    Inductioni

    Induced by illumination and cytokinin treatment in etiolated seedlings. Not induced by phosphate deprivation.2 Publications

    Gene expression databases

    GenevestigatoriO81770.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO81770.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 28 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0707.
    HOGENOMiHOG000239591.
    InParanoidiQ9MU68.
    KOiK03715.
    OMAiIATVMTD.
    PhylomeDBiO81770.

    Family and domain databases

    InterProiIPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view]
    PfamiPF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O81770-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQNPSTVTQE SAAPVFDFFP RLRGLTSRNR SPCSNSDGYA LSSSNALYFN    50
    GFRTLPSRRM GKTLASLSFN TKSSAGSSLR RFISDFNSFI RFHCDKVVPE 100
    SFASVGGVGL SSDENGIREN GTGGVLGEEG LPLNGVEADR PKKVLILMSD 150
    TGGGHRASAE AIRAAFNQEF GDEYQVFITD LWTDHTPWPF NQLPRSYNFL 200
    VKHGTLWKMT YYGTSPRIVH QSNFAATSTF IAREIAQGLM KYQPDIIISV 250
    HPLMQHVPLR VLRSKGLLKK IVFTTVITDL STCHPTWFHK LVTRCYCPST 300
    EVAKRAQKAG LETSQIKVYG LPVRPSFVKP VRPKVELRRE LGMDENLPAV 350
    LLMGGGEGMG PIEATARALA DALYDKNLGE AVGQVLIICG RNKKLQSKLS 400
    SLDWKIPVQV KGFITKMEEC MGACDCIITK AGPGTIAEAM IRGLPIILNG 450
    YIAGQEAGNV PYVVENGCGK FSKSPKEISK IVADWFGPAS KELEIMSQNA 500
    LRLAKPEAVF KIVHDMHELV RKKNSLPQLS CTA 533
    Length:533
    Mass (Da):58,538
    Last modified:November 1, 1998 - v1
    Checksum:iE581E67317CB9CC8
    GO
    Isoform 2 (identifier: O81770-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         456-504: EAGNVPYVVE...IMSQNALRLA → VSRECTVRGG...DNVTECIEAG
         505-533: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:504
    Mass (Da):55,437
    Checksum:i51ED4D5A942E9ACA
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei456 – 50449EAGNV…ALRLA → VSRECTVRGGKRMWEILKIT ERDIEDCSGLVWTGIERVGD NVTECIEAG in isoform 2. CuratedVSP_035387Add
    BLAST
    Alternative sequencei505 – 53329Missing in isoform 2. CuratedVSP_035388Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241797 mRNA. Translation: AAF65066.1.
    AB047399 mRNA. Translation: BAB12042.1.
    AL031004 Genomic DNA. Translation: CAA19745.1.
    AL161579 Genomic DNA. Translation: CAB79896.1.
    CP002687 Genomic DNA. Translation: AEE85956.1.
    CP002687 Genomic DNA. Translation: AEE85957.1.
    AY092965 mRNA. Translation: AAM12964.1.
    BT008890 mRNA. Translation: AAP68329.1.
    PIRiT05092.
    RefSeqiNP_194906.1. NM_119327.2. [O81770-1]
    NP_849482.1. NM_179151.1. [O81770-2]
    UniGeneiAt.1647.

    Genome annotation databases

    EnsemblPlantsiAT4G31780.2; AT4G31780.2; AT4G31780. [O81770-1]
    GeneIDi829306.
    KEGGiath:AT4G31780.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241797 mRNA. Translation: AAF65066.1 .
    AB047399 mRNA. Translation: BAB12042.1 .
    AL031004 Genomic DNA. Translation: CAA19745.1 .
    AL161579 Genomic DNA. Translation: CAB79896.1 .
    CP002687 Genomic DNA. Translation: AEE85956.1 .
    CP002687 Genomic DNA. Translation: AEE85957.1 .
    AY092965 mRNA. Translation: AAM12964.1 .
    BT008890 mRNA. Translation: AAP68329.1 .
    PIRi T05092.
    RefSeqi NP_194906.1. NM_119327.2. [O81770-1 ]
    NP_849482.1. NM_179151.1. [O81770-2 ]
    UniGenei At.1647.

    3D structure databases

    ProteinModelPortali O81770.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT28. Glycosyltransferase Family 28.

    Proteomic databases

    PaxDbi O81770.
    PRIDEi O81770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G31780.2 ; AT4G31780.2 ; AT4G31780 . [O81770-1 ]
    GeneIDi 829306.
    KEGGi ath:AT4G31780.

    Organism-specific databases

    TAIRi AT4G31780.

    Phylogenomic databases

    eggNOGi COG0707.
    HOGENOMi HOG000239591.
    InParanoidi Q9MU68.
    KOi K03715.
    OMAi IATVMTD.
    PhylomeDBi O81770.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2230-MONOMER.
    MetaCyc:AT4G31780-MONOMER.

    Gene expression databases

    Genevestigatori O81770.

    Family and domain databases

    InterProi IPR009695. Diacylglyc_glucosyltr_N.
    IPR007235. Glyco_trans_28_C.
    [Graphical view ]
    Pfami PF04101. Glyco_tran_28_C. 1 hit.
    PF06925. MGDG_synth. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant."
      Jarvis P., Doermann P., Peto C.A., Lutes J., Benning C., Chory J.
      Proc. Natl. Acad. Sci. U.S.A. 97:8175-8179(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    2. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
      Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
      Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. Cited for: FUNCTION, TISSUE SPECIFICITY, CHARACTERIZATION, SUBCELLULAR LOCATION, NOMENCLATURE.
    7. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
      Kobayashi K., Awai K., Takamiya K., Ohta H.
      Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT AND CYTOKININ.
    8. "Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis."
      Kobayashi K., Kondo M., Fukuda H., Nishimura M., Ohta H.
      Proc. Natl. Acad. Sci. U.S.A. 104:17216-17221(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiMGDG1_ARATH
    AccessioniPrimary (citable) accession number: O81770
    Secondary accession number(s): Q3E9T1, Q9MU68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3