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O81770

- MGDG1_ARATH

UniProt

O81770 - MGDG1_ARATH

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Protein
Monogalactosyldiacylglycerol synthase 1, chloroplastic
Gene
MGD1, MGDA, At4g31780, F28M20.30
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes. Required for proper thylakoid membrane biogenesis. Does not discriminate between prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the prokaryotic one.4 Publications

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

pH dependencei

Optimum pH is 7.5.

GO - Molecular functioni

  1. 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: TAIR
  2. UDP-galactosyltransferase activity Source: TAIR
  3. UDP-glycosyltransferase activity Source: TAIR
  4. carbohydrate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glycolipid biosynthetic process Source: TAIR
  2. lipid glycosylation Source: InterPro
  3. thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:GQT-2230-MONOMER.
MetaCyc:AT4G31780-MONOMER.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Monogalactosyldiacylglycerol synthase 1, chloroplastic (EC:2.4.1.46)
Short name:
AtMGD1
Alternative name(s):
MGDG synthase type A
Gene namesi
Name:MGD1
Synonyms:MGDA
Ordered Locus Names:At4g31780
ORF Names:F28M20.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G31780.

Subcellular locationi

Plastidchloroplast inner membrane Inferred 2 Publications

GO - Cellular componenti

  1. chloroplast envelope Source: TAIR
  2. chloroplast inner membrane Source: TAIR
  3. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Plants show defects in chloroplast biogenesis and a dwarf and albino phenotype. The amount of monogalactosyldiacylglycerol was reduced by 98% in the mutant leaves, indicating that MGD2/MGD3 cannot compensate for loss of MGD1 function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 106106Chloroplast Reviewed prediction
Add
BLAST
Chaini107 – 533427Monogalactosyldiacylglycerol synthase 1, chloroplastic
PRO_0000349421Add
BLAST

Proteomic databases

PaxDbiO81770.
PRIDEiO81770.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers, siliques and seeds.3 Publications

Developmental stagei

Highly expressed throughout whole developmental stages. Transient increase in embryos at the globular stage.2 Publications

Inductioni

Induced by illumination and cytokinin treatment in etiolated seedlings. Not induced by phosphate deprivation.2 Publications

Gene expression databases

GenevestigatoriO81770.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO81770.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0707.
HOGENOMiHOG000239591.
InParanoidiQ9MU68.
KOiK03715.
OMAiIATVMTD.
PhylomeDBiO81770.

Family and domain databases

InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O81770-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQNPSTVTQE SAAPVFDFFP RLRGLTSRNR SPCSNSDGYA LSSSNALYFN    50
GFRTLPSRRM GKTLASLSFN TKSSAGSSLR RFISDFNSFI RFHCDKVVPE 100
SFASVGGVGL SSDENGIREN GTGGVLGEEG LPLNGVEADR PKKVLILMSD 150
TGGGHRASAE AIRAAFNQEF GDEYQVFITD LWTDHTPWPF NQLPRSYNFL 200
VKHGTLWKMT YYGTSPRIVH QSNFAATSTF IAREIAQGLM KYQPDIIISV 250
HPLMQHVPLR VLRSKGLLKK IVFTTVITDL STCHPTWFHK LVTRCYCPST 300
EVAKRAQKAG LETSQIKVYG LPVRPSFVKP VRPKVELRRE LGMDENLPAV 350
LLMGGGEGMG PIEATARALA DALYDKNLGE AVGQVLIICG RNKKLQSKLS 400
SLDWKIPVQV KGFITKMEEC MGACDCIITK AGPGTIAEAM IRGLPIILNG 450
YIAGQEAGNV PYVVENGCGK FSKSPKEISK IVADWFGPAS KELEIMSQNA 500
LRLAKPEAVF KIVHDMHELV RKKNSLPQLS CTA 533
Length:533
Mass (Da):58,538
Last modified:November 1, 1998 - v1
Checksum:iE581E67317CB9CC8
GO
Isoform 2 (identifier: O81770-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     456-504: EAGNVPYVVE...IMSQNALRLA → VSRECTVRGG...DNVTECIEAG
     505-533: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:504
Mass (Da):55,437
Checksum:i51ED4D5A942E9ACA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei456 – 50449EAGNV…ALRLA → VSRECTVRGGKRMWEILKIT ERDIEDCSGLVWTGIERVGD NVTECIEAG in isoform 2.
VSP_035387Add
BLAST
Alternative sequencei505 – 53329Missing in isoform 2.
VSP_035388Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF241797 mRNA. Translation: AAF65066.1.
AB047399 mRNA. Translation: BAB12042.1.
AL031004 Genomic DNA. Translation: CAA19745.1.
AL161579 Genomic DNA. Translation: CAB79896.1.
CP002687 Genomic DNA. Translation: AEE85956.1.
CP002687 Genomic DNA. Translation: AEE85957.1.
AY092965 mRNA. Translation: AAM12964.1.
BT008890 mRNA. Translation: AAP68329.1.
PIRiT05092.
RefSeqiNP_194906.1. NM_119327.2. [O81770-1]
NP_849482.1. NM_179151.1. [O81770-2]
UniGeneiAt.1647.

Genome annotation databases

EnsemblPlantsiAT4G31780.2; AT4G31780.2; AT4G31780. [O81770-1]
GeneIDi829306.
KEGGiath:AT4G31780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF241797 mRNA. Translation: AAF65066.1 .
AB047399 mRNA. Translation: BAB12042.1 .
AL031004 Genomic DNA. Translation: CAA19745.1 .
AL161579 Genomic DNA. Translation: CAB79896.1 .
CP002687 Genomic DNA. Translation: AEE85956.1 .
CP002687 Genomic DNA. Translation: AEE85957.1 .
AY092965 mRNA. Translation: AAM12964.1 .
BT008890 mRNA. Translation: AAP68329.1 .
PIRi T05092.
RefSeqi NP_194906.1. NM_119327.2. [O81770-1 ]
NP_849482.1. NM_179151.1. [O81770-2 ]
UniGenei At.1647.

3D structure databases

ProteinModelPortali O81770.
ModBasei Search...

Protein family/group databases

CAZyi GT28. Glycosyltransferase Family 28.

Proteomic databases

PaxDbi O81770.
PRIDEi O81770.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G31780.2 ; AT4G31780.2 ; AT4G31780 . [O81770-1 ]
GeneIDi 829306.
KEGGi ath:AT4G31780.

Organism-specific databases

TAIRi AT4G31780.

Phylogenomic databases

eggNOGi COG0707.
HOGENOMi HOG000239591.
InParanoidi Q9MU68.
KOi K03715.
OMAi IATVMTD.
PhylomeDBi O81770.

Enzyme and pathway databases

BioCyci ARA:GQT-2230-MONOMER.
MetaCyc:AT4G31780-MONOMER.

Gene expression databases

Genevestigatori O81770.

Family and domain databases

InterProi IPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view ]
Pfami PF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant."
    Jarvis P., Doermann P., Peto C.A., Lutes J., Benning C., Chory J.
    Proc. Natl. Acad. Sci. U.S.A. 97:8175-8179(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  2. "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana."
    Awai K., Marechal E., Block M.A., Brun D., Masuda T., Shimada H., Takamiya K., Ohta H., Joyard J.
    Proc. Natl. Acad. Sci. U.S.A. 98:10960-10965(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. Cited for: FUNCTION, TISSUE SPECIFICITY, CHARACTERIZATION, SUBCELLULAR LOCATION, NOMENCLATURE.
  7. "Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation."
    Kobayashi K., Awai K., Takamiya K., Ohta H.
    Plant Physiol. 134:640-648(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY LIGHT AND CYTOKININ.
  8. "Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis."
    Kobayashi K., Kondo M., Fukuda H., Nishimura M., Ohta H.
    Proc. Natl. Acad. Sci. U.S.A. 104:17216-17221(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMGDG1_ARATH
AccessioniPrimary (citable) accession number: O81770
Secondary accession number(s): Q3E9T1, Q9MU68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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