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Protein

Monogalactosyldiacylglycerol synthase 1, chloroplastic

Gene

MGD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the major structural component of photosynthetic membranes. Required for proper thylakoid membrane biogenesis. Does not discriminate between prokaryotic (18:1/16:0) or eukaryotic (18:2/18:2) 1,2-diacylglycerol species, but operates with some preference for the prokaryotic one.4 Publications

Catalytic activityi

UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.

pH dependencei

Optimum pH is 7.5.

GO - Molecular functioni

  • 1,2-diacylglycerol 3-beta-galactosyltransferase activity Source: TAIR
  • UDP-galactosyltransferase activity Source: TAIR
  • UDP-glycosyltransferase activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • glycolipid biosynthetic process Source: TAIR
  • thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:AT4G31780-MONOMER.
BRENDAi2.4.1.46. 399.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Chemistry databases

SwissLipidsiSLP:000001440.

Names & Taxonomyi

Protein namesi
Recommended name:
Monogalactosyldiacylglycerol synthase 1, chloroplastic (EC:2.4.1.46)
Short name:
AtMGD1
Alternative name(s):
MGDG synthase type A
Gene namesi
Name:MGD1
Synonyms:MGDA
Ordered Locus Names:At4g31780
ORF Names:F28M20.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G31780.

Subcellular locationi

GO - Cellular componenti

  • chloroplast envelope Source: TAIR
  • chloroplast inner membrane Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Plants show defects in chloroplast biogenesis and a dwarf and albino phenotype. The amount of monogalactosyldiacylglycerol was reduced by 98% in the mutant leaves, indicating that MGD2/MGD3 cannot compensate for loss of MGD1 function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 106ChloroplastSequence analysisAdd BLAST106
ChainiPRO_0000349421107 – 533Monogalactosyldiacylglycerol synthase 1, chloroplasticAdd BLAST427

Proteomic databases

PaxDbiO81770.

PTM databases

iPTMnetiO81770.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers, siliques and seeds.3 Publications

Developmental stagei

Highly expressed throughout whole developmental stages. Transient increase in embryos at the globular stage.2 Publications

Inductioni

Induced by illumination and cytokinin treatment in etiolated seedlings. Not induced by phosphate deprivation.2 Publications

Gene expression databases

ExpressionAtlasiO81770. baseline and differential.
GenevisibleiO81770. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G31780.2.

Structurei

Secondary structure

1533
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi143 – 147Combined sources5
Turni150 – 153Combined sources4
Helixi156 – 170Combined sources15
Helixi171 – 173Combined sources3
Beta strandi175 – 178Combined sources4
Helixi232 – 242Combined sources11
Beta strandi245 – 249Combined sources5
Turni252 – 255Combined sources4
Helixi256 – 263Combined sources8
Turni264 – 266Combined sources3
Turni268 – 270Combined sources3
Beta strandi271 – 276Combined sources6
Beta strandi280 – 282Combined sources3
Helixi285 – 287Combined sources3
Beta strandi293 – 296Combined sources4
Helixi300 – 309Combined sources10
Helixi313 – 315Combined sources3
Beta strandi316 – 318Combined sources3
Turni325 – 327Combined sources3
Helixi334 – 341Combined sources8
Beta strandi345 – 347Combined sources3
Beta strandi349 – 353Combined sources5
Turni355 – 358Combined sources4
Helixi362 – 372Combined sources11
Turni376 – 379Combined sources4
Beta strandi380 – 382Combined sources3
Beta strandi384 – 388Combined sources5
Helixi393 – 401Combined sources9
Beta strandi408 – 412Combined sources5
Helixi417 – 422Combined sources6
Beta strandi425 – 429Combined sources5
Helixi433 – 441Combined sources9
Beta strandi446 – 448Combined sources3
Turni453 – 458Combined sources6
Helixi459 – 464Combined sources6
Turni465 – 467Combined sources3
Beta strandi468 – 471Combined sources4
Helixi475 – 485Combined sources11
Turni486 – 489Combined sources4
Helixi490 – 503Combined sources14
Helixi508 – 520Combined sources13
Turni521 – 523Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WYIX-ray2.50A137-533[»]
4X1TX-ray2.25A137-533[»]
ProteinModelPortaliO81770.
SMRiO81770.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IG4I. Eukaryota.
COG0707. LUCA.
HOGENOMiHOG000239591.
InParanoidiO81770.
KOiK03715.
OMAiWGEFARF.
OrthoDBiEOG09360AEE.
PhylomeDBiO81770.

Family and domain databases

InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O81770-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQNPSTVTQE SAAPVFDFFP RLRGLTSRNR SPCSNSDGYA LSSSNALYFN
60 70 80 90 100
GFRTLPSRRM GKTLASLSFN TKSSAGSSLR RFISDFNSFI RFHCDKVVPE
110 120 130 140 150
SFASVGGVGL SSDENGIREN GTGGVLGEEG LPLNGVEADR PKKVLILMSD
160 170 180 190 200
TGGGHRASAE AIRAAFNQEF GDEYQVFITD LWTDHTPWPF NQLPRSYNFL
210 220 230 240 250
VKHGTLWKMT YYGTSPRIVH QSNFAATSTF IAREIAQGLM KYQPDIIISV
260 270 280 290 300
HPLMQHVPLR VLRSKGLLKK IVFTTVITDL STCHPTWFHK LVTRCYCPST
310 320 330 340 350
EVAKRAQKAG LETSQIKVYG LPVRPSFVKP VRPKVELRRE LGMDENLPAV
360 370 380 390 400
LLMGGGEGMG PIEATARALA DALYDKNLGE AVGQVLIICG RNKKLQSKLS
410 420 430 440 450
SLDWKIPVQV KGFITKMEEC MGACDCIITK AGPGTIAEAM IRGLPIILNG
460 470 480 490 500
YIAGQEAGNV PYVVENGCGK FSKSPKEISK IVADWFGPAS KELEIMSQNA
510 520 530
LRLAKPEAVF KIVHDMHELV RKKNSLPQLS CTA
Length:533
Mass (Da):58,538
Last modified:November 1, 1998 - v1
Checksum:iE581E67317CB9CC8
GO
Isoform 2 (identifier: O81770-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     456-504: EAGNVPYVVE...IMSQNALRLA → VSRECTVRGG...DNVTECIEAG
     505-533: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:504
Mass (Da):55,437
Checksum:i51ED4D5A942E9ACA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035387456 – 504EAGNV…ALRLA → VSRECTVRGGKRMWEILKIT ERDIEDCSGLVWTGIERVGD NVTECIEAG in isoform 2. CuratedAdd BLAST49
Alternative sequenceiVSP_035388505 – 533Missing in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241797 mRNA. Translation: AAF65066.1.
AB047399 mRNA. Translation: BAB12042.1.
KJ138730 mRNA. Translation: AHL38670.1.
AL031004 Genomic DNA. Translation: CAA19745.1.
AL161579 Genomic DNA. Translation: CAB79896.1.
CP002687 Genomic DNA. Translation: AEE85956.1.
CP002687 Genomic DNA. Translation: AEE85957.1.
AY092965 mRNA. Translation: AAM12964.1.
BT008890 mRNA. Translation: AAP68329.1.
PIRiT05092.
RefSeqiNP_194906.1. NM_119327.3. [O81770-1]
UniGeneiAt.1647.

Genome annotation databases

EnsemblPlantsiAT4G31780.2; AT4G31780.2; AT4G31780. [O81770-1]
GeneIDi829306.
GrameneiAT4G31780.2; AT4G31780.2; AT4G31780.
KEGGiath:AT4G31780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241797 mRNA. Translation: AAF65066.1.
AB047399 mRNA. Translation: BAB12042.1.
KJ138730 mRNA. Translation: AHL38670.1.
AL031004 Genomic DNA. Translation: CAA19745.1.
AL161579 Genomic DNA. Translation: CAB79896.1.
CP002687 Genomic DNA. Translation: AEE85956.1.
CP002687 Genomic DNA. Translation: AEE85957.1.
AY092965 mRNA. Translation: AAM12964.1.
BT008890 mRNA. Translation: AAP68329.1.
PIRiT05092.
RefSeqiNP_194906.1. NM_119327.3. [O81770-1]
UniGeneiAt.1647.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WYIX-ray2.50A137-533[»]
4X1TX-ray2.25A137-533[»]
ProteinModelPortaliO81770.
SMRiO81770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G31780.2.

Chemistry databases

SwissLipidsiSLP:000001440.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

PTM databases

iPTMnetiO81770.

Proteomic databases

PaxDbiO81770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G31780.2; AT4G31780.2; AT4G31780. [O81770-1]
GeneIDi829306.
GrameneiAT4G31780.2; AT4G31780.2; AT4G31780.
KEGGiath:AT4G31780.

Organism-specific databases

TAIRiAT4G31780.

Phylogenomic databases

eggNOGiENOG410IG4I. Eukaryota.
COG0707. LUCA.
HOGENOMiHOG000239591.
InParanoidiO81770.
KOiK03715.
OMAiWGEFARF.
OrthoDBiEOG09360AEE.
PhylomeDBiO81770.

Enzyme and pathway databases

BioCyciMetaCyc:AT4G31780-MONOMER.
BRENDAi2.4.1.46. 399.

Miscellaneous databases

PROiO81770.

Gene expression databases

ExpressionAtlasiO81770. baseline and differential.
GenevisibleiO81770. AT.

Family and domain databases

InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGDG1_ARATH
AccessioniPrimary (citable) accession number: O81770
Secondary accession number(s): Q3E9T1, Q9MU68, W8Q2X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.