O81716 (P2C21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 21
Short name=AtPP2C21
EC=3.1.3.16

Alternative name(s):
AtPPC4;2

Gene names

Name:	PPC4-2
Ordered Locus Names:	At2g25070
ORF Names:	F13D4.1, F27C12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro response to abscisic acid stimulus Inferred from direct assay PubMed 20733066. Source: TAIR
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Probable protein phosphatase 2C 21		PRO_0000367951
Regions
	Domain	22 – 322	301	PP2C-like
Sites
	Metal binding	57	1	Manganese 1 By similarity
	Metal binding	57	1	Manganese 2 By similarity
	Metal binding	58	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	320	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O81716 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CAD638796203C746	FASTA	355	39,354
	10 20 30 40 50 60 MGTYLSSPKT EKLSEDGEND KLRFGLSSMQ GWRATMEDAH AAILDLDDKT SFFGVYDGHG 70 80 90 100 110 120 GKVVAKFCAK YLHQQVISNE AYKTGDVETS LRRAFFRMDD MMQGQRGWRE LAVLGDKMNK 130 140 150 160 170 180 FSGMIEGFIW SPRSGDTNNQ PDSWPLEDGP HSDFTGPTSG CTACVALIKD KKLFVANAGD 190 200 210 220 230 240 SRCVISRKSQ AYNLSKDHKP DLEVEKERIL KAGGFIHAGR INGSLNLTRA IGDMEFKQNK 250 260 270 280 290 300 FLPSEKQMVT ADPDINTIDL CDDDDFLVVA CDGIWDCMSS QELVDFIHEQ LKSETKLSTV 310 320 330 340 350 CEKVVDRCLA PDTATGEGCD NMTIILVQFK KPNPSETEPE DSKPEPSEDE PSSSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana." Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB079671 mRNA. Translation: BAB84700.1. AC006585 Genomic DNA. Translation: AAD23006.1. CP002685 Genomic DNA. Translation: AEC07654.1. AY050873 mRNA. Translation: AAK92810.1. AY091209 mRNA. Translation: AAM14148.1.
IPI	IPI00516376.
PIR	H84643.
RefSeq	NP_180079.1. NM_128064.2.
UniGene	At.24404.
3D structure databases
ProteinModelPortal	O81716.
SMR	O81716. Positions 1-330.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8390663.
STRING	3702.AT2G25070.1-P.
Proteomic databases
PaxDb	O81716.
PRIDE	O81716.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G25070.1; AT2G25070.1; AT2G25070.
GeneID	817045.
KEGG	ath:AT2G25070.
Organism-specific databases
TAIR	At2g25070.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	O81716.
KO	K01090.
OMA	HAGRING.
PhylomeDB	O81716.
ProtClustDB	CLSN2683143.
Gene expression databases
Genevestigator	O81716.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C21_ARATH

Accession

Primary (citable) accession number: O81716

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 1, 1998
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program