Reviewed, UniProtKB/Swiss-Prot O81701 (POLC4_ALNGL)

Names and origin

Protein names	Recommended name:     Polcalcin Aln g 4 Alternative name(s):     Calcium-binding pollen allergen Aln g 4     Allergen=Aln g 4
Organism	Alnus glutinosa (Alder)
Taxonomic identifier	3517 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fagales › Betulaceae › Alnus

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Contains 2 EF-hand domains.
Mass spectrometry	Molecular mass is 9318.6 Da from positions 1 - 85. Determined by MALDI. Ref.1

Ontologies

Keywords
Disease	Allergen
Domain	Repeat
Ligand	Calcium
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Polcalcin Aln g 4		PRO_0000073663
Regions
	Domain	7 – 42	36	EF-hand 1
	Domain	42 – 77	36	EF-hand 2
	Calcium binding	20 – 31	12	1 Potential
	Calcium binding	55 – 66	12	2 Potential

Sequences

	Sequence		Length	Mass (Da)
	O81701-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 903774CC1F714FAD	FASTA	85	9,362
	10 20 30 40 50 60 MADDHPQDQA EHERIFKCFD ANGDGKISAS ELGDALKTLG SVTPDEVKHM MAEIDTDGDG 70 80 FISFQEFTNF ARANRGLVKD VAKIF

References

[1]

"Molecular and immunologic characterization of a highly cross-reactive two EF-hand calcium-binding alder pollen allergen, Aln g 4: structural basis for calcium-modulated IgE recognition." Hayek B., Vangelista L., Pastore A., Sperr W.R., Valent P., Vrtala S., Niederberger V., Twardosz A., Kraft D., Valenta R. J. Immunol. 161:7031-7039(1998) [PubMed: 9862740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MASS SPECTROMETRY. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17713 mRNA. Translation: CAA76831.1.
3D structure databases
SMR	O81701. Positions 2-85.
ModBase	Search...
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR018248. EF_Hand_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 2 hits. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLC4_ALNGL

Accession

Primary (citable) accession number: O81701

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1998
Last modified:	November 24, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)