O81701 (POLC4_ALNGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polcalcin Aln g 4 Alternative name(s): Calcium-binding pollen allergen Aln g 4 Allergen=Aln g 4
Organism	Alnus glutinosa (European alder) (Betula alnus var. glutinosa)
Taxonomic identifier	3517 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fagales › Betulaceae › Alnus

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Contains 2 EF-hand domains.
Mass spectrometry	Molecular mass is 9318.6 Da from positions 1 - 85. Determined by MALDI. Ref.1

Ontologies

Keywords
Disease	Allergen
Domain	Repeat
Ligand	Calcium Metal-binding
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Polcalcin Aln g 4		PRO_0000073663
Regions
	Domain	7 – 42	36	EF-hand 1
	Domain	42 – 77	36	EF-hand 2
	Calcium binding	20 – 31	12	1 Potential
	Calcium binding	55 – 66	12	2 Potential

Sequences

	Sequence		Length	Mass (Da)
	O81701 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 903774CC1F714FAD	FASTA	85	9,362
	10 20 30 40 50 60 MADDHPQDQA EHERIFKCFD ANGDGKISAS ELGDALKTLG SVTPDEVKHM MAEIDTDGDG 70 80 FISFQEFTNF ARANRGLVKD VAKIF

References

[1]

"Molecular and immunologic characterization of a highly cross-reactive two EF-hand calcium-binding alder pollen allergen, Aln g 4: structural basis for calcium-modulated IgE recognition." Hayek B., Vangelista L., Pastore A., Sperr W.R., Valent P., Vrtala S., Niederberger V., Twardosz A., Kraft D., Valenta R. J. Immunol. 161:7031-7039(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MASS SPECTROMETRY. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17713 mRNA. Translation: CAA76831.1.
3D structure databases
ProteinModelPortal	O81701.
SMR	O81701. Positions 2-85.
ModBase	Search...
Protein family/group databases
Allergome	3056. Aln g 4.0101. 9. Aln g 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POLC4_ALNGL

Accession

Primary (citable) accession number: O81701

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program