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Protein

Alpha-amylase

Gene

alpha-Amy2A

Organism
Avena fatua (Wild oats)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031NucleophileUniRule annotation
Active sitei228 – 2281Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationImported, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Name:alpha-Amy2AImported
OrganismiAvena fatua (Wild oats)Imported
Taxonomic identifieri4499 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaePoodaePoeaeAveninaeAvena

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 434411Alpha-amylaseSequence analysisPRO_5004160190Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO81699.
SMRiO81699. Positions 24-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 366342AamyInterPro annotationAdd
BLAST
Domaini367 – 42761Alpha-amyl_C2InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

O81699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKNSHLCCF SLLLLLLAGL ASGHQVLFQG FNWESWKQSG GWYNMMMGKV
60 70 80 90 100
DDIAAAGVTH VWLPPPSHSV SNEGYMPGRL YDIDASKYGN AAELKSLIGA
110 120 130 140 150
FHGKGVHVIA DIVINHRCAD YKDKRGIYCI FEGGTPDSRL DWGPHMICRD
160 170 180 190 200
DTIYSDGTAN LDTGADFAAA PDIDHLNDRV QRELKEWLLW LKTDLGFDAW
210 220 230 240 250
RLDFAKGYSA AMAKVYIDGT SPDLAVAEVW DTMQTGGDGK PEYDQDAHRQ
260 270 280 290 300
NLVNWVDGVG GAASAGMVFD FTTKGILNAA VEGELWRLID PQGKAPGVMG
310 320 330 340 350
WWPAKAVTFV DNHDTGSTQA MWPFPSDKVM QGYAYILTHP GTPCIFYDHF
360 370 380 390 400
FNWGFKEEIA ALVAVRKRNG ITATSELKIL MHDGDAYVAE IDGKVVVKIG
410 420 430
SRYDVGSVIP AGFVTSAHGS DYAVWEKSAT LQRS
Length:434
Mass (Da):47,642
Last modified:November 1, 1998 - v1
Checksum:i499F8C3E9767C1E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010728 Genomic DNA. Translation: CAA09323.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010728 Genomic DNA. Translation: CAA09323.1.

3D structure databases

ProteinModelPortaliO81699.
SMRiO81699. Positions 24-427.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Willmott R.L.
    Thesis (1994), University of Bristol
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "DNase1 footprints suggest the involvement of at least three types of transcription factors in the regulation of alpha-Amy2/A by gibberellin."
    Willmott R.L., Rushton P.J., Hooley R., Lazarus C.M.
    Plant Mol. Biol. 38:817-825(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiO81699_AVEFA
AccessioniPrimary (citable) accession number: O81699
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.