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Protein

Villin-3

Gene

VLN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds actin and actin filament bundles in a Ca2+-insensitive manner, but severs actin filaments in a calcium-dependent manner, regardless of the presence or not of VLN1 (AC O81643). Acts redundantly with VLN2 (AC O81644) to generate thick actin filament bundles, to regulate directional organ growth (PubMed:22209875) and in sclerenchyma development (PubMed:22563899).4 Publications

GO - Molecular functioni

  • actin filament binding Source: TAIR

GO - Biological processi

  • actin filament bundle assembly Source: TAIR
  • actin filament capping Source: UniProtKB-KW
  • actin filament severing Source: TAIR

Keywordsi

Molecular functionActin capping, Actin-binding
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Villin-31 Publication
Gene namesi
Name:VLN31 Publication
Ordered Locus Names:At3g57410Imported
ORF Names:F28O9.260Imported, T8H10.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G57410
TAIRilocus:2082523 AT3G57410

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Vln2 and vln3 double mutants show absence of thick actin filament bundles in the cells, anomaly in the growth direction of organs (PubMed:22209875) and defects in sclerenchyma development, but no alterations in the secondary cell-wall machinery (PubMed:22563899).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187341 – 965Villin-3Add BLAST965

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei880PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81645
PRIDEiO81645

PTM databases

iPTMnetiO81645

Expressioni

Tissue specificityi

Expressed in all tissues examined, including root hairs.4 Publications

Gene expression databases

ExpressionAtlasiO81645 baseline and differential
GenevisibleiO81645 AT

Interactioni

GO - Molecular functioni

  • actin filament binding Source: TAIR

Protein-protein interaction databases

BioGridi10224, 2 interactors
STRINGi3702.AT3G57410.1

Structurei

3D structure databases

ProteinModelPortaliO81645
SMRiO81645
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 79Gelsolin-like 1Sequence analysisAdd BLAST53
Repeati150 – 190Gelsolin-like 2Sequence analysisAdd BLAST41
Repeati262 – 304Gelsolin-like 3Sequence analysisAdd BLAST43
Repeati401 – 452Gelsolin-like 4Sequence analysisAdd BLAST52
Repeati533 – 573Gelsolin-like 5Sequence analysisAdd BLAST41
Repeati635 – 676Gelsolin-like 6Sequence analysisAdd BLAST42
Domaini900 – 965HPPROSITE-ProRule annotationAdd BLAST66

Domaini

The HP domain is important for the localization to actin filament bundles.1 Publication

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443 Eukaryota
ENOG410XR0A LUCA
HOGENOMiHOG000233631
InParanoidiO81645
OMAiECFILDH
OrthoDBiEOG0936017U

Family and domain databases

Gene3Di1.10.950.10, 1 hit
3.40.20.10, 6 hits
InterProiView protein in InterPro
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR007123 Gelsolin-like_dom
IPR030009 Villin-2/3/4/5_plant
IPR007122 Villin/Gelsolin
IPR003128 Villin_headpiece
IPR036886 Villin_headpiece_dom_sf
PANTHERiPTHR11977 PTHR11977, 1 hit
PTHR11977:SF40 PTHR11977:SF40, 1 hit
PfamiView protein in Pfam
PF00626 Gelsolin, 6 hits
PF02209 VHP, 1 hit
PRINTSiPR00597 GELSOLIN
SMARTiView protein in SMART
SM00262 GEL, 6 hits
SM00153 VHP, 1 hit
SUPFAMiSSF47050 SSF47050, 1 hit
PROSITEiView protein in PROSITE
PS51089 HP, 1 hit

Sequencei

Sequence statusi: Complete.

O81645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSTKVLDP AFQGVGQKPG TEIWRIENFE PVPVPKSEHG KFYMGDTYIV
60 70 80 90 100
LQTTQNKGGA YLFDIHFWIG KDTSQDEAGT AAVKTVELDA ALGGRAVQYR
110 120 130 140 150
EIQGHESDKF LSYFKPCIIP LEGGVASGFK KPEEEEFETR LYTCKGKRAV
160 170 180 190 200
HLKQVPFARS SLNHDDVFIL DTKEKIYQFN GANSNIQERA KALVVIQYLK
210 220 230 240 250
DKFHEGTSDV AIVDDGKLDT ESDSGEFWVL FGGFAPIARK VASEDEIIPE
260 270 280 290 300
TTPPKLYSIA DGQVESIDGD LSKSMLENNK CYLLDCGSEI FIWVGRVTQV
310 320 330 340 350
EERKTAIQAA EDFVASENRP KATRITRVIQ GYEPHSFKSN FDSWPSGSAT
360 370 380 390 400
PANEEGRGKV AALLKQQGVG LKGLSKSTPV NEDIPPLLEG GGKLEVWYID
410 420 430 440 450
ANSKTVLSKD HVGKLYSGDC YLVLYTYHSG ERKEDYFLCC WFGKNSNQED
460 470 480 490 500
QETAVRLAST MTNSLKGRPV QARIFEGKEP PQFVALFQHM VVLKGGLSSG
510 520 530 540 550
YKNSMTEKGS SGETYTPESI ALIQVSGTGV HNNKALQVEA VATSLNSYDC
560 570 580 590 600
FLLQSGTSMF LWVGNHSTHE QQELAAKVAE FLKPGTTIKH AKEGTESSSF
610 620 630 640 650
WFALGGKQNF TSKKVSSETV RDPHLFSFSF NRGKFQVEEI HNFDQDDLLT
660 670 680 690 700
EEMHLLDTHA EVFVWVGQCV DPKEKQTAFE IGQRYINLAG SLEGLSPKVP
710 720 730 740 750
LYKITEGNEP CFFTTYFSWD STKATVQGNS YQKKAALLLG THHVVEDQSS
760 770 780 790 800
SGNQGPRQRA AALAALTSAF NSSSGRTSSP SRDRSNGSQG GPRQRAEALA
810 820 830 840 850
ALTSAFNSSP SSKSPPRRSG LTSQASQRAA AVAALSQVLT AEKKKSPDTS
860 870 880 890 900
PSAEAKDEKA FSEVEATEEA TEAKEEEEVS PAAEASAEEA KPKQDDSEVE
910 920 930 940 950
TTGVTFTYER LQAKSEKPVT GIDFKRREAY LSEVEFKTVF GMEKESFYKL
960
PGWKQDLLKK KFNLF
Length:965
Mass (Da):106,349
Last modified:January 25, 2012 - v2
Checksum:i1571DB7D6EF1B6A8
GO

Sequence cautioni

The sequence CAB66098 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB68147 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti859 – 860KA → ETS in AAC31607 (PubMed:10631247).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081203 mRNA Translation: AAC31607.1
AL133248 Genomic DNA Translation: CAB66098.1 Sequence problems.
AL137080 Genomic DNA Translation: CAB68147.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE79652.1
CP002686 Genomic DNA Translation: ANM63565.1
CP002686 Genomic DNA Translation: ANM63566.1
CP002686 Genomic DNA Translation: ANM63567.1
CP002686 Genomic DNA Translation: ANM63568.1
CP002686 Genomic DNA Translation: ANM63569.1
CP002686 Genomic DNA Translation: ANM63570.1
CP002686 Genomic DNA Translation: ANM63572.1
AK318626 mRNA Translation: BAH56741.1
PIRiT50668
RefSeqiNP_001319779.1, NM_001339858.1
NP_001325645.1, NM_001339860.1
NP_001325646.1, NM_001339866.1
NP_001325647.1, NM_001339863.1
NP_001325648.1, NM_001339862.1
NP_001325649.1, NM_001339865.1
NP_001325651.1, NM_001339861.1
NP_567048.1, NM_115601.3
UniGeneiAt.22370

Genome annotation databases

EnsemblPlantsiAT3G57410.1; AT3G57410.1; AT3G57410
AT3G57410.2; AT3G57410.2; AT3G57410
AT3G57410.3; AT3G57410.3; AT3G57410
AT3G57410.4; AT3G57410.4; AT3G57410
AT3G57410.5; AT3G57410.5; AT3G57410
AT3G57410.7; AT3G57410.7; AT3G57410
AT3G57410.8; AT3G57410.8; AT3G57410
AT3G57410.9; AT3G57410.9; AT3G57410
GeneIDi824908
GrameneiAT3G57410.1; AT3G57410.1; AT3G57410
AT3G57410.2; AT3G57410.2; AT3G57410
AT3G57410.3; AT3G57410.3; AT3G57410
AT3G57410.4; AT3G57410.4; AT3G57410
AT3G57410.5; AT3G57410.5; AT3G57410
AT3G57410.7; AT3G57410.7; AT3G57410
AT3G57410.8; AT3G57410.8; AT3G57410
AT3G57410.9; AT3G57410.9; AT3G57410
KEGGiath:AT3G57410

Similar proteinsi

Entry informationi

Entry nameiVILI3_ARATH
AccessioniPrimary (citable) accession number: O81645
Secondary accession number(s): A0A1I9LLW4
, C0Z212, Q9LEA4, Q9SCN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: January 25, 2012
Last modified: April 25, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health