Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Villin-2

Gene

VLN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-regulated actin-binding protein. Involved in actin filaments bundling. Caps the barbed end of actin filaments and is able to sever them in a calcium-dependent manner. Required for the construction of actin collars in pollen tubes. Acts redundantly with VLN5 (AC Q9LVC6) to generate thick actin filament bundles and to regulate polarized pollen tube growth (PubMed:23715472). Acts redundantly with VLN3 (AC O81645) to regulate directional organ growth and in sclerenchyma development (PubMed:22209875, PubMed:22563899, respectively).4 Publications

GO - Biological processi

  • actin filament bundle assembly Source: UniProtKB
  • actin filament capping Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Ligandi

Actin-binding, Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Villin-21 Publication
Gene namesi
Name:VLN21 Publication
Ordered Locus Names:At2g41740Imported
ORF Names:T11A7.16Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41740.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

No visible phenotype and no visible effect on pollen tube growth. Decreased severing frequency of actin filaments. Vln2 and vln5 double mutants have pollen tubes curled and wider at some regions along the tube. They accumulate actin filaments at the tips of pollen tubes (PubMed:23715472). Vln2 and vln3 double mutants show anomaly in the growth direction of organs (PubMed:22209875) and defects in sclerenchyma development, but no alterations in the secondary cell-wall machinery (PubMed:22563899).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187331 – 976Villin-2Add BLAST976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei890PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81644.
PRIDEiO81644.

PTM databases

iPTMnetiO81644.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Mainly detected in the root epidermis and vasculature. Expressed in the root cap.3 Publications

Gene expression databases

GenevisibleiO81644. AT.

Interactioni

Protein-protein interaction databases

BioGridi4110. 1 interactor.
IntActiO81644. 1 interactor.
STRINGi3702.AT2G41740.1.

Structurei

3D structure databases

ProteinModelPortaliO81644.
SMRiO81644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 77Gelsolin-like 1Sequence analysisAdd BLAST51
Repeati148 – 188Gelsolin-like 2Sequence analysisAdd BLAST41
Repeati260 – 302Gelsolin-like 3Sequence analysisAdd BLAST43
Repeati399 – 450Gelsolin-like 4Sequence analysisAdd BLAST52
Repeati531 – 571Gelsolin-like 5Sequence analysisAdd BLAST41
Repeati633 – 674Gelsolin-like 6Sequence analysisAdd BLAST42
Domaini911 – 976HPPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 6 gelsolin-like repeats.Curated
Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO81644.
OMAiCDGSENT.
OrthoDBiEOG0936017U.
PhylomeDBiO81644.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030009. Villin-2/3/4/5_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF46. PTHR11977:SF46. 2 hits.
PfamiPF00626. Gelsolin. 5 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O81644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKVLDPAF QGAGQKPGTE IWRIENFEAV PVPKSEHGKF YMGDTYIVLQ
60 70 80 90 100
TTQNKGGAYL FDIHFWIGKD TSQDEAGTAA VKTVELDAVL GGRAVQHREI
110 120 130 140 150
QGHESDKFLS YFKPCIIPLE GGVASGFKTV EEEVFETRLY TCKGKRAIRL
160 170 180 190 200
KQVPFARSSL NHDDVFILDT EEKIYQFNGA NSNIQERAKA LEVVQYLKDK
210 220 230 240 250
YHEGTCDVAI VDDGKLDTES DSGAFWVLFG GFAPIGRKVA NDDDIVPEST
260 270 280 290 300
PPKLYCITDG KMEPIDGDLS KSMLENTKCY LLDCGAEIYI WVGRVTQVDE
310 320 330 340 350
RKAASQSAEE FLASENRPKA THVTRVIQGY ESHSFKSNFD SWPSGSATPG
360 370 380 390 400
NEEGRGKVAA LLKQQGVGLK GIAKSAPVNE DIPPLLESGG KLEVWYVNGK
410 420 430 440 450
VKTPLPKEDI GKLYSGDCYL VLYTYHSGER KDEYFLSCWF GKKSIPEDQD
460 470 480 490 500
TAIRLANTMS NSLKGRPVQG RIYEGKEPPQ FVALFQPMVV LKGGLSSGYK
510 520 530 540 550
SSMGESESTD ETYTPESIAL VQVSGTGVHN NKAVQVETVA TSLNSYECFL
560 570 580 590 600
LQSGTSMFLW HGNQSTHEQL ELATKVAEFL KPGITLKHAK EGTESSTFWF
610 620 630 640 650
ALGGKQNFTS KKASSETIRD PHLFSFAFNR GKFQVEEIYN FAQDDLLTED
660 670 680 690 700
IYFLDTHAEV FVWVGQCVEP KEKQTVFEIG QKYIDLAGSL EGLHPKVPIY
710 720 730 740 750
KINEGNEPCF FTTYFSWDAT KAIVQGNSFQ KKASLLFGTH HVVEDKSNGG
760 770 780 790 800
NQGLRQRAEA LAALNSAFNS SSNRPAYSSQ DRLNESHDGP RQRAEALAAL
810 820 830 840 850
SSAFNSSSSS TKSPPPPRPV GTSQASQRAA AVAALSQVLV AENKKSPDTS
860 870 880 890 900
PTRRSTSSNP ADDIPLTEAK DEEEASEVAG LEAKEEEEVS PAADETEAKQ
910 920 930 940 950
ETEEQGDSEI QPSGATFTYE QLRAKSENPV TGIDFKRREA YLSEEEFQSV
960 970
FGIEKEAFNN LPRWKQDLLK KKFDLF
Length:976
Mass (Da):107,842
Last modified:November 15, 2002 - v2
Checksum:iF10BAB629A0A957E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99E → V in AAC31606 (PubMed:10631247).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081202 mRNA. Translation: AAC31606.1.
AC002339 Genomic DNA. Translation: AAC02774.2.
CP002685 Genomic DNA. Translation: AEC10027.1.
AY080601 mRNA. Translation: AAL85012.1.
AY133782 mRNA. Translation: AAM91716.1.
AK226882 mRNA. Translation: BAE98961.1.
PIRiE84845.
T50669.
RefSeqiNP_565958.1. NM_129738.5.
UniGeneiAt.26112.
At.67830.

Genome annotation databases

EnsemblPlantsiAT2G41740.1; AT2G41740.1; AT2G41740.
GeneIDi818773.
GrameneiAT2G41740.1; AT2G41740.1; AT2G41740.
KEGGiath:AT2G41740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081202 mRNA. Translation: AAC31606.1.
AC002339 Genomic DNA. Translation: AAC02774.2.
CP002685 Genomic DNA. Translation: AEC10027.1.
AY080601 mRNA. Translation: AAL85012.1.
AY133782 mRNA. Translation: AAM91716.1.
AK226882 mRNA. Translation: BAE98961.1.
PIRiE84845.
T50669.
RefSeqiNP_565958.1. NM_129738.5.
UniGeneiAt.26112.
At.67830.

3D structure databases

ProteinModelPortaliO81644.
SMRiO81644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4110. 1 interactor.
IntActiO81644. 1 interactor.
STRINGi3702.AT2G41740.1.

PTM databases

iPTMnetiO81644.

Proteomic databases

PaxDbiO81644.
PRIDEiO81644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41740.1; AT2G41740.1; AT2G41740.
GeneIDi818773.
GrameneiAT2G41740.1; AT2G41740.1; AT2G41740.
KEGGiath:AT2G41740.

Organism-specific databases

TAIRiAT2G41740.

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO81644.
OMAiCDGSENT.
OrthoDBiEOG0936017U.
PhylomeDBiO81644.

Miscellaneous databases

PROiO81644.

Gene expression databases

GenevisibleiO81644. AT.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030009. Villin-2/3/4/5_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF46. PTHR11977:SF46. 2 hits.
PfamiPF00626. Gelsolin. 5 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVILI2_ARATH
AccessioniPrimary (citable) accession number: O81644
Secondary accession number(s): O22946, Q0WV87, Q8RXZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.