Villin-2 - Arabidopsis thaliana (Mouse-ear cress)

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O81644 (VILI2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Villin-2

Gene names

Name:	VLN2
Ordered Locus Names:	At2g41740
ORF Names:	T11A7.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	976 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ca²⁺-regulated actin-binding protein.
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Expressed in all tissues examined. Ref.1
Sequence similarities	Belongs to the villin/gelsolin family. Contains 6 gelsolin-like repeats. Contains 1 HP (headpiece) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat
Ligand	Actin-binding Calcium
Molecular function	Actin capping
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament bundle assembly Inferred from genetic interaction PubMed 22209875. Source: UniProtKB actin filament capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 976

976

Villin-2

PRO_0000218733

Regions

Repeat

27 – 77

Gelsolin-like 1

Repeat

148 – 188

Gelsolin-like 2

Repeat

260 – 302

Gelsolin-like 3

Repeat

399 – 450

Gelsolin-like 4

Repeat

531 – 571

Gelsolin-like 5

Repeat

633 – 674

Gelsolin-like 6

Domain

911 – 976

Region

113 – 120

Polyphosphoinositide binding By similarity

Amino acid modifications

Modified residue

846

Phosphoserine Ref.5

Modified residue

849

Phosphothreonine Ref.5

Modified residue

850

Phosphoserine Ref.5

Modified residue

855

Phosphoserine Ref.5

Modified residue

856

Phosphothreonine Ref.5

Modified residue

858

Phosphoserine Ref.5

Experimental info

Sequence conflict

E → V in AAC31606. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O81644 [UniParc].

Last modified November 15, 2002. Version 2.
Checksum: F10BAB629A0A957E
Show »

FASTA

976

107,842

        10         20         30         40         50         60 
MSTKVLDPAF QGAGQKPGTE IWRIENFEAV PVPKSEHGKF YMGDTYIVLQ TTQNKGGAYL 

        70         80         90        100        110        120 
FDIHFWIGKD TSQDEAGTAA VKTVELDAVL GGRAVQHREI QGHESDKFLS YFKPCIIPLE 

       130        140        150        160        170        180 
GGVASGFKTV EEEVFETRLY TCKGKRAIRL KQVPFARSSL NHDDVFILDT EEKIYQFNGA 

       190        200        210        220        230        240 
NSNIQERAKA LEVVQYLKDK YHEGTCDVAI VDDGKLDTES DSGAFWVLFG GFAPIGRKVA 

       250        260        270        280        290        300 
NDDDIVPEST PPKLYCITDG KMEPIDGDLS KSMLENTKCY LLDCGAEIYI WVGRVTQVDE 

       310        320        330        340        350        360 
RKAASQSAEE FLASENRPKA THVTRVIQGY ESHSFKSNFD SWPSGSATPG NEEGRGKVAA 

       370        380        390        400        410        420 
LLKQQGVGLK GIAKSAPVNE DIPPLLESGG KLEVWYVNGK VKTPLPKEDI GKLYSGDCYL 

       430        440        450        460        470        480 
VLYTYHSGER KDEYFLSCWF GKKSIPEDQD TAIRLANTMS NSLKGRPVQG RIYEGKEPPQ 

       490        500        510        520        530        540 
FVALFQPMVV LKGGLSSGYK SSMGESESTD ETYTPESIAL VQVSGTGVHN NKAVQVETVA 

       550        560        570        580        590        600 
TSLNSYECFL LQSGTSMFLW HGNQSTHEQL ELATKVAEFL KPGITLKHAK EGTESSTFWF 

       610        620        630        640        650        660 
ALGGKQNFTS KKASSETIRD PHLFSFAFNR GKFQVEEIYN FAQDDLLTED IYFLDTHAEV 

       670        680        690        700        710        720 
FVWVGQCVEP KEKQTVFEIG QKYIDLAGSL EGLHPKVPIY KINEGNEPCF FTTYFSWDAT 

       730        740        750        760        770        780 
KAIVQGNSFQ KKASLLFGTH HVVEDKSNGG NQGLRQRAEA LAALNSAFNS SSNRPAYSSQ 

       790        800        810        820        830        840 
DRLNESHDGP RQRAEALAAL SSAFNSSSSS TKSPPPPRPV GTSQASQRAA AVAALSQVLV 

       850        860        870        880        890        900 
AENKKSPDTS PTRRSTSSNP ADDIPLTEAK DEEEASEVAG LEAKEEEEVS PAADETEAKQ 

       910        920        930        940        950        960 
ETEEQGDSEI QPSGATFTYE QLRAKSENPV TGIDFKRREA YLSEEEFQSV FGIEKEAFNN 

       970 
LPRWKQDLLK KKFDLF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis." Klahre U., Friederich E., Kost B., Louvard D., Chua N.-H. Plant Physiol. 122:35-47(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-846; THR-849; SER-850; SER-855; THR-856 AND SER-858, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081202 mRNA. Translation: AAC31606.1. AC002339 Genomic DNA. Translation: AAC02774.2. CP002685 Genomic DNA. Translation: AEC10027.1. AY080601 mRNA. Translation: AAL85012.1. AY133782 mRNA. Translation: AAM91716.1.
IPI	IPI00520251.
PIR	E84845. T50669.
RefSeq	NP_565958.1. NM_129738.4.
UniGene	At.26112. At.67830.
3D structure databases
ProteinModelPortal	O81644.
SMR	O81644. Positions 8-721, 926-976.
ModBase	Search...
Protein-protein interaction databases
IntAct	O81644. 1 interaction.
Proteomic databases
PaxDb	O81644.
PRIDE	O81644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G41740.1; AT2G41740.1; AT2G41740.
GeneID	818773.
KEGG	ath:AT2G41740.
Organism-specific databases
TAIR	At2g41740.
Phylogenomic databases
eggNOG	NOG304849.
HOGENOM	HOG000233631.
InParanoid	O81644.
OMA	KFDATSM.
PhylomeDB	O81644.
ProtClustDB	CLSN2685608.
Gene expression databases
ArrayExpress	O81644.
Genevestigator	O81644.
GermOnline	AT2G41740. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.950.10. 1 hit.
InterPro	IPR007123. Gelsolin_dom. IPR007122. Villin/Gelsolin. IPR003128. Villin_headpiece. [Graphical view]
PANTHER	PTHR11977. PTHR11977. 1 hit.
Pfam	PF00626. Gelsolin. 5 hits. PF02209. VHP. 1 hit. [Graphical view]
PRINTS	PR00597. GELSOLIN.
SMART	SM00262. GEL. 6 hits. SM00153. VHP. 1 hit. [Graphical view]
SUPFAM	SSF47050. VHP. 1 hit.
PROSITE	PS51089. HP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VILI2_ARATH

Accession

Primary (citable) accession number: O81644
Secondary accession number(s): O22946, Q8RXZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents