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Protein

Villin-1

Gene

VLN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Binds actin and actin filament bundles in a Ca2+/calmodulin-insensitive manner, but is unable to sever, cap, and nucleate actin filament formation in vitro. Does not protect individual filaments from severing by VLN3 (AC O81645).3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Actin capping

Keywords - Ligandi

Actin-binding, Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Villin-11 Publication
Gene namesi
Name:VLN11 Publication
Ordered Locus Names:At2g29890Imported
ORF Names:F6K5.2Imported, T27A16.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G29890.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 909909Villin-1PRO_0000218732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei780 – 7801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO81643.
PRIDEiO81643.

PTM databases

iPTMnetiO81643.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Mainly detected in the vascular tissue and the pericycle of roots and in the vasculature of leaves. Not expressed in the root cap.2 Publications

Gene expression databases

ExpressionAtlasiO81643. baseline and differential.
GenevisibleiO81643. AT.

Interactioni

Protein-protein interaction databases

BioGridi2889. 1 interaction.
STRINGi3702.AT2G29890.3.

Structurei

3D structure databases

ProteinModelPortaliO81643.
SMRiO81643. Positions 12-719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 7951Gelsolin-like 1Sequence analysisAdd
BLAST
Repeati149 – 18941Gelsolin-like 2Sequence analysisAdd
BLAST
Repeati262 – 30544Gelsolin-like 3Sequence analysisAdd
BLAST
Repeati391 – 44858Gelsolin-like 4Sequence analysisAdd
BLAST
Repeati529 – 56941Gelsolin-like 5Sequence analysisAdd
BLAST
Repeati631 – 67242Gelsolin-like 6Sequence analysisAdd
BLAST
Domaini844 – 90966HPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 6 gelsolin-like repeats.Curated
Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO81643.
PhylomeDBiO81643.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030010. Villin-1_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF25. PTHR11977:SF25. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O81643-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRLSKDIDS AFQGVGTKSG LEIWCVYNKQ LISIPKSSFG KFHSGNAYLV
60 70 80 90 100
LRTFLRKIES PQYDIHYWLG IDANEVDSIL ASDKALDLDA ALGCCTVQYR
110 120 130 140 150
EVQGQETEKF LSYFKPCIIP VEGKYSPKTG IAGETYQVTL LRCKGDHVVR
160 170 180 190 200
VKEVPFLRSS LNHDDVFILD TASKVFLFAG CNSSTQEKAK AMEVVEYIKD
210 220 230 240 250
NKHDGRCEVA TIEDGKFSGD SDAGEFWSFF GGYAPIPKLS SSTTQEQTQT
260 270 280 290 300
PCAELFWIDT KGNLHPTGTS SLDKDMLEKN KCYMLDCHSE VFVWMGRNTS
310 320 330 340 350
LTERKTSISS SEEFLRKEGR STTTSLVLLT EGLENARFRS FFNKWPQTVE
360 370 380 390 400
SSLYNEGREK VAALFKQKGY DVEELPDEED DPLYTNCRDN LKVWRVDGDD
410 420 430 440 450
VSLLSIPDQT KLFTGDCYLV QYKYTYKERT EHLLYVWIGC ESIQQDRADA
460 470 480 490 500
ITNASAIVGT TKGESVLCHI YQGNEPSRFF PMFQSLVVFK GGLSRRYKVL
510 520 530 540 550
LAEKEKIGEE YNENKASLFR VVGTSPRNMQ AIQVNLVATS LNSSYSYILQ
560 570 580 590 600
YGASAFTWIG KLSSDSDHEV LDRMLYFLDT SCQPIYIREG NETDTFWNLL
610 620 630 640 650
GGKSEYPKEK EMRKQIEEPH LFTCSCSSDV LKVKEIYNFV QDDLTTEDVF
660 670 680 690 700
LLDCQSEVYV WIGSNSNIKS KEEALTLGLK FLEMDILEEG LTMRTPVYVV
710 720 730 740 750
TEGHEPPFFT RFFEWVPEKA NMHGNSFERK LASLKGKKTS TKRSSGSQYR
760 770 780 790 800
SQSKDNASRD LQSRSVSSNG SERGVSPCSS EKLLSLSSAE DMTNSSNSTP
810 820 830 840 850
VVKKLFSESL LVDPNDGVAR QESSSKSDIS KQKPRVGINS DLSSLESLAY
860 870 880 890 900
SYEQLRVDSQ KPVTDIDATR REAYLTEKEF EERFGMAKSE FYALPKWKQN

KLKISLHLF
Length:909
Mass (Da):102,950
Last modified:May 15, 2007 - v2
Checksum:i4F04501BC4523FCC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81I → F in AAC31605 (PubMed:10631247).Curated
Sequence conflicti347 – 3537QTVESSL → SDRWSLAF in AAC31605 (PubMed:10631247).Curated
Sequence conflicti585 – 5851I → T in AAC31605 (PubMed:10631247).Curated
Sequence conflicti811 – 8111L → V in AAC31605 (PubMed:10631247).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081201 mRNA. Translation: AAC31605.1.
AC007113 Genomic DNA. Translation: AAD23629.2.
CP002685 Genomic DNA. Translation: AEC08315.1.
PIRiH84701.
T50671.
RefSeqiNP_029567.1. NM_128543.3. [O81643-1]
UniGeneiAt.11948.

Genome annotation databases

EnsemblPlantsiAT2G29890.1; AT2G29890.1; AT2G29890. [O81643-1]
GeneIDi817539.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081201 mRNA. Translation: AAC31605.1.
AC007113 Genomic DNA. Translation: AAD23629.2.
CP002685 Genomic DNA. Translation: AEC08315.1.
PIRiH84701.
T50671.
RefSeqiNP_029567.1. NM_128543.3. [O81643-1]
UniGeneiAt.11948.

3D structure databases

ProteinModelPortaliO81643.
SMRiO81643. Positions 12-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2889. 1 interaction.
STRINGi3702.AT2G29890.3.

PTM databases

iPTMnetiO81643.

Proteomic databases

PaxDbiO81643.
PRIDEiO81643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G29890.1; AT2G29890.1; AT2G29890. [O81643-1]
GeneIDi817539.

Organism-specific databases

TAIRiAT2G29890.

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
HOGENOMiHOG000233631.
InParanoidiO81643.
PhylomeDBiO81643.

Miscellaneous databases

PROiO81643.

Gene expression databases

ExpressionAtlasiO81643. baseline and differential.
GenevisibleiO81643. AT.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 6 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR030010. Villin-1_plant.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 2 hits.
PTHR11977:SF25. PTHR11977:SF25. 2 hits.
PfamiPF00626. Gelsolin. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 6 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis."
    Klahre U., Friederich E., Kost B., Louvard D., Chua N.-H.
    Plant Physiol. 122:35-47(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis VILLIN1 generates actin filament cables that are resistant to depolymerization."
    Huang S., Robinson R.C., Gao L.Y., Matsumoto T., Brunet A., Blanchoin L., Staiger C.J.
    Plant Cell 17:486-501(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover."
    Khurana P., Henty J.L., Huang S., Staiger A.M., Blanchoin L., Staiger C.J.
    Plant Cell 22:2727-2748(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVILI1_ARATH
AccessioniPrimary (citable) accession number: O81643
Secondary accession number(s): O82367, Q9SIK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 15, 2007
Last modified: January 20, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.