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Protein
Submitted name:

Pullulanase-type starch debranching enzyme

Gene

zpu1

Organism
Zea mays (Maize)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BRENDAi3.2.1.41. 6752.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Pullulanase-type starch debranching enzymeImported
Submitted name:
Uncharacterized proteinImported
Gene namesi
Name:zpu1Imported
Synonyms:Zpu1Imported
OrganismiZea mays (Maize)Imported
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 2

Expressioni

Gene expression databases

ExpressionAtlasiO81638. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G158043_P02.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 29085CBM_48InterPro annotationAdd
BLAST
Domaini790 – 960171DUF3372InterPro annotationAdd
BLAST

Phylogenomic databases

eggNOGiENOG410IN7N. Eukaryota.
COG1523. LUCA.
HOGENOMiHOG000251916.
OMAiSWEGCYY.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 3 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR011839. Pullul_strch.
IPR024561. Pullul_strch_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 5 hits.
PfamiPF02922. CBM_48. 1 hit.
PF11852. DUF3372. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR02103. pullul_strch. 1 hit.

Sequencei

Sequence statusi: Complete.

O81638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLHAGPSFL LAPPPRFAAA PSSASPRRSR TPQSSPPTSH FARPADPVAQ
60 70 80 90 100
RVRPVAPRPP MATAEEGASS DVGVAVAESA QGFLLDARAY WVTKSLIAWN
110 120 130 140 150
ISDQKTSLFL YASRNATMCM SSQDMKGYDS KVELQPENDG LPSSVTQKFP
160 170 180 190 200
FISSYRAFRI PSSVDVATLV KCQLAVASFD AHGNRQDVTG LQLPGVLDDM
210 220 230 240 250
FAYTGPLGTI FSEEAVSMYL WAPTAQDVSV SFYDGPAGPL LETVQLNELN
260 270 280 290 300
GVWSVTGPRN WENRYYLYEV TVYHQTTGNI EKCLAADPYA RGLSANSTRT
310 320 330 340 350
WLVDINNETL KPLAWDGLAA EKPRLDSFSD ISIYELHIRD FSAHDSTVDC
360 370 380 390 400
PFRGGFCAFT FQDSVGIEHL KKLSDAGLTH VHLLPSFQFG GVDDIKSNWK
410 420 430 440 450
CVDEIELSKL PPGSDLQQAA IVAIQEEDPY NWGYNPVVWG VPKGSYASNP
460 470 480 490 500
DGPSRIIEYR LMVQALNRLG LRVVMDVVYN HLYSSGPFAI TSVLDKIVPG
510 520 530 540 550
YYLRRDSNGQ TENSAAVNNT ASEHFMVDRL IVDDLLNWAV NYKVDGFRFD
560 570 580 590 600
LMGHIMKKTM IRAKSALQSL TIDEHGVDGS KIYLYGEGWN FGEVAENQRG
610 620 630 640 650
INGSQLNMSG TGIGSFNDRI RDAINGGSPF GNPLQQGFST GLFLEPNGFY
660 670 680 690 700
QGNETETRLT LATYADHIQI GLAGNLKDYV VISHTGEARK GSEIRTFDGS
710 720 730 740 750
PVGYASSPIE TINYASAHDN ETLFDIISLK TPMDLSIDER CRINHLSTSM
760 770 780 790 800
IALSQGIPFF HAGDEILRSK SLDRDSYDSG DWFNKIDFTY ETNNWGVGLP
810 820 830 840 850
PREKNEGSWP LMKPRLENPS FKPAKHDIIA ALDKFIDILK IRYSSPLFRL
860 870 880 890 900
TTASDIVQRV HFHNTGPSLV PGVIVMSIED ARNDRHDMAQ IDETFSCVVT
910 920 930 940 950
VFNVCPYEVS IEIPDLASLR LQLHPVQVNS SDALARQSAY DTATGRFTVP
960
KRTAAVFVEP RC
Length:962
Mass (Da):106,240
Last modified:November 1, 1998 - v1
Checksum:i5F5AAB7C6EBD5D10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080567 mRNA. Translation: AAD11599.1.
KP872821 mRNA. Translation: AKO22325.1.
PIRiT01688.
RefSeqiNP_001104920.1. NM_001111450.1.
UniGeneiZm.78576.

Genome annotation databases

EnsemblPlantsiGRMZM2G158043_T02; GRMZM2G158043_P02; GRMZM2G158043.
GeneIDi541711.
GrameneiGRMZM2G158043_T02; GRMZM2G158043_P02; GRMZM2G158043.
KEGGizma:541711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080567 mRNA. Translation: AAD11599.1.
KP872821 mRNA. Translation: AKO22325.1.
PIRiT01688.
RefSeqiNP_001104920.1. NM_001111450.1.
UniGeneiZm.78576.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G158043_P02.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM2G158043_T02; GRMZM2G158043_P02; GRMZM2G158043.
GeneIDi541711.
GrameneiGRMZM2G158043_T02; GRMZM2G158043_P02; GRMZM2G158043.
KEGGizma:541711.

Phylogenomic databases

eggNOGiENOG410IN7N. Eukaryota.
COG1523. LUCA.
HOGENOMiHOG000251916.
OMAiSWEGCYY.

Enzyme and pathway databases

BRENDAi3.2.1.41. 6752.

Gene expression databases

ExpressionAtlasiO81638. baseline and differential.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 3 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR011839. Pullul_strch.
IPR024561. Pullul_strch_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 5 hits.
PfamiPF02922. CBM_48. 1 hit.
PF11852. DUF3372. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR02103. pullul_strch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and molecular genetic characterization of ZPU1, a pullulanase-type starch-debranching enzyme from maize."
    Beatty M.K., Rahman A., Cao H., Woodman W., Lee M., Myers A.M., James M.G.
    Plant Physiol. 119:255-266(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: EndospermImported.
  2. "The B73 maize genome: complexity, diversity, and dynamics."
    Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S., Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D., Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K., Fronick C.
    , Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M., Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B., Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E., Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J., Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E., Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A., Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R., Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A., Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E., Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A., Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T., Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L., Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P., Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A., Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C., Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A., Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C., SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W., Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C., Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K., Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K., Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S., Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.
    Science 326:1112-1115(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. B73Imported.
  3. "Pullulanase and Starch Synthase III Are Associated with Formation of Vitreous Endosperm in Quality Protein Maize."
    Wu H., Clay K., Thompson S.S., Hennen-Bierwagen T.A., Andrews B.J., Zechmann B., Gibbon B.C.
    PLoS ONE 10:E0130856-E0130856(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Line W64Ao2Imported.
  4. EnsemblPlants
    Submitted (FEB-2015) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: cv. B73Imported.

Entry informationi

Entry nameiO81638_MAIZE
AccessioniPrimary (citable) accession number: O81638
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.