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Protein

PHD finger protein ALFIN-LIKE 4

Gene

AL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209Histone H3K4me3By similarity1
Binding sitei215Histone H3K4me3By similarity1
Binding sitei219Histone H3K4me3By similarity1
Binding sitei224Histone H3K4me3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 251PHD-typePROSITE-ProRule annotationAdd BLAST53

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • methylated histone binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger protein ALFIN-LIKE 4
Short name:
Protein AL4
Gene namesi
Name:AL4
Ordered Locus Names:At5g26210
ORF Names:F9D12.13, T19G15_60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G26210
TAIRilocus:2179709 AT5G26210

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593361 – 255PHD finger protein ALFIN-LIKE 4Add BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO81488

PTM databases

iPTMnetiO81488

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

ExpressionAtlasiO81488 baseline and differential
GenevisibleiO81488 AT

Interactioni

Subunit structurei

Interacts with H3K4me3 and to a lesser extent with H3K4me2.1 Publication

GO - Molecular functioni

  • methylated histone binding Source: TAIR

Protein-protein interaction databases

BioGridi17965, 1 interactor
STRINGi3702.AT5G26210.1

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi203 – 205Combined sources3
Beta strandi211 – 213Combined sources3
Beta strandi215 – 217Combined sources3
Beta strandi219 – 221Combined sources3
Beta strandi224 – 226Combined sources3
Turni227 – 231Combined sources5
Helixi236 – 239Combined sources4
Helixi246 – 249Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE9NMR-A201-251[»]
ProteinModelPortaliO81488
SMRiO81488
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO81488

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi177 – 199Glu-richAdd BLAST23

Domaini

The PHD-type zinc finger mediates the binding to H3K4me3.1 Publication

Sequence similaritiesi

Belongs to the Alfin family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri199 – 251PHD-typePROSITE-ProRule annotationAdd BLAST53

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1632 Eukaryota
ENOG410YG6P LUCA
HOGENOMiHOG000238550
InParanoidiO81488
OMAiCCDLCEM
OrthoDBiEOG09360O5K
PhylomeDBiO81488

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR021998 Alfin
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF12165 Alfin, 1 hit
PF00628 PHD, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequencei

Sequence statusi: Complete.

O81488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAGGAYNPR TVEEVFRDFK GRRAGMIKAL TTDVQEFFRL CDPEKENLCL
60 70 80 90 100
YGHPNEHWEV NLPAEEVPPE LPEPVLGINF ARDGMAEKDW LSLVAVHSDA
110 120 130 140 150
WLLAVAFFFG ARFGFDKADR KRLFNMVNDL PTIFEVVAGT AKKQGKDKSS
160 170 180 190 200
VSNNSSNRSK SSSKRGSESR AKFSKPEPKD DEEEEEEGVE EEDEDEQGET
210 220 230 240 250
QCGACGESYA ADEFWICCDL CEMWFHGKCV KITPARAEHI KQYKCPSCSN

KRARS
Length:255
Mass (Da):28,779
Last modified:August 30, 2005 - v2
Checksum:i077571EBCB255BB0
GO

Sequence cautioni

The sequence AAC26230 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186E → G in BAE98515 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077407 Genomic DNA Translation: AAC26230.1 Sequence problems.
CP002688 Genomic DNA Translation: AED93537.1
BT024475 mRNA Translation: ABD19656.1
AK226367 mRNA Translation: BAE98515.1
AB493760 mRNA Translation: BAH30598.1
AY087173 mRNA Translation: AAM64729.1
PIRiT01840
RefSeqiNP_197993.1, NM_122522.5
UniGeneiAt.49064
At.68496

Genome annotation databases

EnsemblPlantsiAT5G26210.1; AT5G26210.1; AT5G26210
GeneIDi832690
GrameneiAT5G26210.1; AT5G26210.1; AT5G26210
KEGGiath:AT5G26210

Similar proteinsi

Entry informationi

Entry nameiALFL4_ARATH
AccessioniPrimary (citable) accession number: O81488
Secondary accession number(s): Q0WWI3, Q2HIV6, Q8LBJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health